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The map label for this gene is napA [H]

Identifier: 157960452

GI number: 157960452

Start: 765981

End: 768461

Strand: Reverse

Name: napA [H]

Synonym: Spea_0623

Alternate gene names: 157960452

Gene position: 768461-765981 (Counterclockwise)

Preceding gene: 157960453

Following gene: 157960451

Centisome position: 14.85

GC content: 48.49

Gene sequence:

>2481_bases
ATGAGCATTAGCCGTCGCGAGTTTCTAAAAGCCAACGCCGCCGTTGCAGCCGCAACTGCCGTAGGCGCGACTCTGCCAGT
TAAGATAGTCGAAGCTGCAGAGCAAAAAGATAACATCAAGTGGGACAAAGCACCTTGTCGTTTTTGTGGCGTAGGTTGTA
GTGTTCTTGTAGGCACAGATAATGGCAAAGTCGTCGCCACCAAAGGTGACCCTGAAAGCCCTGTGAACAAAGGCCTTAAC
TGTATTAAAGGCTACTTCCTTTCTAAAATCATGTACGGTAAGGATCGCCTAACCACACCGCTTCTACGTATGAAAGACGG
CCAATATGATAAAGAAGGCGAGTTCACCCCAATCAGCTGGGATAGCGCTTTTGACATCATGGCTGACAAATGGAAAAACA
CCCTTAAGACCAAAGGCCCAACTGCCATTGGTATGTTTGGCTCTGGCCAGTGGACTGTTTGGGAAGGTTATGCTGCATCT
AAACTTCACAAAGCCGGTTTCCTAACTAACAACATCGACCCGAATGCGCGTCACTGTATGGCATCTGCGGTTGTTGGCTT
TATGCGTACCTTTGGTATCGATGAGCCAATGGGTTGTTACGACGACTTAGAAGCCGCTGACCAGTTCGTGCTATGGGGCG
CGAACATGGCTGAAATGCACCCAATCCTTTGGGCTCGCCTATCGGATCGTCGCCTAAGTAGCCCGACTAGCCGCGTGCAC
GTGTTATCGACTTTCGAAAACCGTAGCTTCGACTTAGCCGACAACGCCATGGTGTTCCGTCCACAGTCTGACTTAGTGAT
CCTTAACTATATTGCTAACTACATCATTCAAAACGATGCGGTTAACAAAGACTTCGTTAACGAACATACCACCTTTGCAC
TAGGTACTACCGATATCGGTTATGGTCTGCGTCCAGATCACCCGCTTGAGAAAAAAGCTAAGAACCCAGGTAACGGTAAA
TTCGCACCGATCAGCTTCGATGAGTACGCTAAGTTTGTTAGCACATACACGCTAGAATACGCAGCAGAGATGAGTGGTGT
TGAGCCTGAAAAGCTTGAGCTAATGGCTAAGGCTTATGCCGATCCAAACGTGAAGATGATGAGTCTGTGGACCATGGGTA
TCAACCAACACACTCGTGGTGTTTGGGCCAACAACATGCTTTACAACATCCACTTGCTAACCGGTAAAATTGCCACTCCA
GGCAACAGCCCATTCTCGCTAACGGGTCAACCATCTGCTTGTGGTACTGCACGTGAAGTAGGTACGTTCGCGCACCGCCT
ACCAGCAGACATGGTAGTCATGAATCCTAAGCACCGTGCAATTGCTGAGAAGTTATGGCAACTACCAGAAGGTACGATTC
CGCCAAAGCCTGGTTTCCACGCCGTGCTACAGAGCCGCATGCTTAAAGATGGCAAGCTGAACTGTTACTGGACTATGTGT
ACCAATAACATGCAAGCGGGTCCAAACATTAACGATGAAATGTACCCAGGTTTCCGTAACCCAGAAAACTTCATTGTGGT
ATCTGATCCCTACCCAACAGTAACCGCTATGGCTGCTGACTTGATTCTACCAACGGCTATGTGGGTAGAAAAAGAAGGGG
CTTACGGCAACGCAGAGCGTCGCACTCACATGTGGCACCAGCAAGTTAAAGCACCAGAGGGCGCTAAGTCTGACCTATGG
CAGCTTATGGAATTCTCTAAGCGCTTTAAGGTGTCTGAAGTCTGGCCAGCGGAGCTTATTGCTAAGCAACCTGAAGTGGC
TGACAAGACATTATTTGATGTGCTTTACGCCAATGGCGTAGTCAACAAGTTCCCTGCTTCTGATTGCAAAGCTGAATTGA
ACGACGAGTCTGATGCATTTGGTTTCTACGTACAAAAAGGACTATTTGAAGAATACGCCCAGTTTGGTCGTGGTCACGCT
CACGATTTAGCAGACTTCGATACTTACCATGAGACTCGCGGCCTACGCTGGCCGGTAGTTGATGGTAAAGAAACATTACG
TCGCTTCTCGAAAGGCGATCCGTATGTGAAAGGCGACAAGGAATTTGACTTCTACGGTAAGCCTGACGGTAAAGCGGTGA
TTTTCGCCCTACCATTTGAGCCTGCAGCAGAAGAGCCAAATGAAGAATACGATCTATGGTTATCAACCGGCCGTGTTCTT
GAACATTGGCACACAGGTTCGATGACAGCTCGCGTACCTGAGCTTTATCGCGCATACCCTGATGCACAAATCTTTATGCA
CCCAGAAGATGCTAAAGCACGTGGTCTAAAGCGTGGCGATGAAGTGATTGTGGCTTCGCCTCGCGGTGAAGTAAAAACCC
GTGTAGAAACTAAAGGCCGTAACAAGCCGCCAAGAGGCGTGGCGTTTATGCCATTCTTCGATGCGCGCCAGCTGGTCAAC
AAGTTAATACTAGATGCGACTGATCCGCTGTCAAAAGAGACAGACTTCAAGAAGTGTCCAGTTAAAGTGATGAAAGCGTA
A

Upstream 100 bases:

>100_bases
CTCGATAGCGTAGAAGCGATTAACGCCTTAGAAGGCGTGTTAAATAGCAGTCTAATTTATCATCAAGTCGATCCAGTAGA
AGAAAAGAGTGAGGAACACC

Downstream 100 bases:

>100_bases
GCCTTTCACTAACAAATTTAACTTTAATCGCTTAAGCAAAAGCGGAGAAGAGATAAGCAAATGAGTACGGTCGATCAACG
TAAGTTCGCAGCCAAAGGCG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 826; Mature: 825

Protein sequence:

>826_residues
MSISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTDNGKVVATKGDPESPVNKGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISWDSAFDIMADKWKNTLKTKGPTAIGMFGSGQWTVWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
VLSTFENRSFDLADNAMVFRPQSDLVILNYIANYIIQNDAVNKDFVNEHTTFALGTTDIGYGLRPDHPLEKKAKNPGNGK
FAPISFDEYAKFVSTYTLEYAAEMSGVEPEKLELMAKAYADPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNPKHRAIAEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLW
QLMEFSKRFKVSEVWPAELIAKQPEVADKTLFDVLYANGVVNKFPASDCKAELNDESDAFGFYVQKGLFEEYAQFGRGHA
HDLADFDTYHETRGLRWPVVDGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDLWLSTGRVL
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGRNKPPRGVAFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVMKA

Sequences:

>Translated_826_residues
MSISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTDNGKVVATKGDPESPVNKGLN
CIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISWDSAFDIMADKWKNTLKTKGPTAIGMFGSGQWTVWEGYAAS
KLHKAGFLTNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
VLSTFENRSFDLADNAMVFRPQSDLVILNYIANYIIQNDAVNKDFVNEHTTFALGTTDIGYGLRPDHPLEKKAKNPGNGK
FAPISFDEYAKFVSTYTLEYAAEMSGVEPEKLELMAKAYADPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATP
GNSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNPKHRAIAEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLW
QLMEFSKRFKVSEVWPAELIAKQPEVADKTLFDVLYANGVVNKFPASDCKAELNDESDAFGFYVQKGLFEEYAQFGRGHA
HDLADFDTYHETRGLRWPVVDGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDLWLSTGRVL
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGRNKPPRGVAFMPFFDARQLVN
KLILDATDPLSKETDFKKCPVKVMKA
>Mature_825_residues
SISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTDNGKVVATKGDPESPVNKGLNC
IKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISWDSAFDIMADKWKNTLKTKGPTAIGMFGSGQWTVWEGYAASK
LHKAGFLTNNIDPNARHCMASAVVGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVHV
LSTFENRSFDLADNAMVFRPQSDLVILNYIANYIIQNDAVNKDFVNEHTTFALGTTDIGYGLRPDHPLEKKAKNPGNGKF
APISFDEYAKFVSTYTLEYAAEMSGVEPEKLELMAKAYADPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNPKHRAIAEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMCT
NNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAERRTHMWHQQVKAPEGAKSDLWQ
LMEFSKRFKVSEVWPAELIAKQPEVADKTLFDVLYANGVVNKFPASDCKAELNDESDAFGFYVQKGLFEEYAQFGRGHAH
DLADFDTYHETRGLRWPVVDGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDLWLSTGRVLE
HWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGRNKPPRGVAFMPFFDARQLVNK
LILDATDPLSKETDFKKCPVKVMKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=827, Percent_Identity=72.3095525997582, Blast_Score=1265, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=787, Percent_Identity=24.5235069885642, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI3868720, Length=603, Percent_Identity=23.5489220563847, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI3868719, Length=487, Percent_Identity=24.6406570841889, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI87081797, Length=829, Percent_Identity=21.2303980699638, Blast_Score=93, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92364; Mature: 92233

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTD
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECC
NGKVVATKGDPESPVNKGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCCCCC
DSAFDIMADKWKNTLKTKGPTAIGMFGSGQWTVWEGYAASKLHKAGFLTNNIDPNARHCM
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVVGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
VLSTFENRSFDLADNAMVFRPQSDLVILNYIANYIIQNDAVNKDFVNEHTTFALGTTDIG
EEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCHHHCCCCCEEEEECCCCC
YGLRPDHPLEKKAKNPGNGKFAPISFDEYAKFVSTYTLEYAAEMSGVEPEKLELMAKAYA
CCCCCCCCHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMVVMNPKHRAIAEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGAKSDLWQLMEFSKRFKVSEVWPAELIAKQPEVADKTLFDVLYANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
VNKFPASDCKAELNDESDAFGFYVQKGLFEEYAQFGRGHAHDLADFDTYHETRGLRWPVV
CCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
DGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDLWLSTGRVL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCHHH
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGR
HHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCCC
NKPPRGVAFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVMKA
CCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
SISRREFLKANAAVAAATAVGATLPVKIVEAAEQKDNIKWDKAPCRFCGVGCSVLVGTD
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEECC
NGKVVATKGDPESPVNKGLNCIKGYFLSKIMYGKDRLTTPLLRMKDGQYDKEGEFTPISW
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCCCCCCCCCCCCC
DSAFDIMADKWKNTLKTKGPTAIGMFGSGQWTVWEGYAASKLHKAGFLTNNIDPNARHCM
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCEECCCCCCHHHHH
ASAVVGFMRTFGIDEPMGCYDDLEAADQFVLWGANMAEMHPILWARLSDRRLSSPTSRVH
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
VLSTFENRSFDLADNAMVFRPQSDLVILNYIANYIIQNDAVNKDFVNEHTTFALGTTDIG
EEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHCCCCCCCHHHCCCCCEEEEECCCCC
YGLRPDHPLEKKAKNPGNGKFAPISFDEYAKFVSTYTLEYAAEMSGVEPEKLELMAKAYA
CCCCCCCCHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNVKMMSLWTMGINQHTRGVWANNMLYNIHLLTGKIATPGNSPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCCCCCCEEECCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCHHHH
GTFAHRLPADMVVMNPKHRAIAEKLWQLPEGTIPPKPGFHAVLQSRMLKDGKLNCYWTMC
HHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEE
TNNMQAGPNINDEMYPGFRNPENFIVVSDPYPTVTAMAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHH
RTHMWHQQVKAPEGAKSDLWQLMEFSKRFKVSEVWPAELIAKQPEVADKTLFDVLYANGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
VNKFPASDCKAELNDESDAFGFYVQKGLFEEYAQFGRGHAHDLADFDTYHETRGLRWPVV
CCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
DGKETLRRFSKGDPYVKGDKEFDFYGKPDGKAVIFALPFEPAAEEPNEEYDLWLSTGRVL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCHHH
EHWHTGSMTARVPELYRAYPDAQIFMHPEDAKARGLKRGDEVIVASPRGEVKTRVETKGR
HHHCCCCCCHHHHHHHHHCCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCEEEHHHCCC
NKPPRGVAFMPFFDARQLVNKLILDATDPLSKETDFKKCPVKVMKA
CCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA