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The map label for this gene is yafC [C]

Identifier: 157960433

GI number: 157960433

Start: 742513

End: 743424

Strand: Reverse

Name: yafC [C]

Synonym: Spea_0604

Alternate gene names: 157960433

Gene position: 743424-742513 (Counterclockwise)

Preceding gene: 157960435

Following gene: 157960432

Centisome position: 14.37

GC content: 46.93

Gene sequence:

>912_bases
ATGCCAGATTTAAACGGTATGATGCTCTTTGCGGCGGTAGTTAGAGCCAAAGGTTTCTCTCAAGCTGCCCGTGAAATAGG
CATGCCAAAATCAACCATTAGTCGTAAGGTTGCCCAGCTAGAAGAGCAGCTTGGCGTGCGACTATTGCAGCGAGATACCC
GTAATCTAAGTTTGACTCAGGTCGGTGCGCTGTTTTTTCAGCATTGCTCCAGTATAAGCGATGAAATTGAAGCTGCAAAA
GCCACTGTGGAAAATACCCATAACGATGTCTCAGGCCTGTTGCGTATCGCGATTCCAGTCTCTTTCAGTCAAGAGCTAAT
CGCCACCTTGTGTAGCGGTTTTCTGCGCCTCTATCCCAATATCGAGTTAGATGTGCAGTTTACCGATAGTGATGTAGGAC
TCGTTGGGGAAGGCTATGATATCGCTATCAAATACGGTCCACTGCAATCATCGGATCTTGTCGCCAGACTGCTATTTGAA
CGCCAACCCATATTGGTCGCAAGTCCCGGCTACCTGAAAATTCATGGTACACCTGCCACACCTCAAGATTTAGCCGAACA
CAGCGGTATTTTGCTGGGAACCTCTCGCTCGGCGCCAATCTGGCCCCTAGGCAAAGGCGCCAGAAAAACCATGGTTAACT
TCCAGCGTAAGGTTCGAGTCAATAGTGCTGTGATGGTTAAACAGTTGGTGATGGATGATTTTGGCATTGCCATGCTGTCT
AATTCAGTGTGTAAGAACGAACTGGCGAGTGGCTCTTTGGTGCCCATTCTACAAGAGTGGCCGATGGAAGCGTTTAAGGT
ATATGGCGTGTATTCAAGCAGACGTCAACTAGCGACTAATATCAGTGTATTTTTGGATTTCTTTCATAAGCGTTTCACCA
ACCAAGAATCCTTACAATCTATGATGATGTAG

Upstream 100 bases:

>100_bases
CTCTGAAATTTTTAGCCCGTAAATATAACTTTGTTATCCCGTCAATTGCTATTAAACTGCACTAGACGCTTTGTTCCATA
TTTGAGACAGTGGAGATAAC

Downstream 100 bases:

>100_bases
TCTCAATTTACAGCGCGCGGCAGTAACTGCCGAAGCTCTTATAAATTAGCTCCTCGGTATTGGAGCTTTTCAAGCTGACC
TCATTAGTCATGATTACCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MPDLNGMMLFAAVVRAKGFSQAAREIGMPKSTISRKVAQLEEQLGVRLLQRDTRNLSLTQVGALFFQHCSSISDEIEAAK
ATVENTHNDVSGLLRIAIPVSFSQELIATLCSGFLRLYPNIELDVQFTDSDVGLVGEGYDIAIKYGPLQSSDLVARLLFE
RQPILVASPGYLKIHGTPATPQDLAEHSGILLGTSRSAPIWPLGKGARKTMVNFQRKVRVNSAVMVKQLVMDDFGIAMLS
NSVCKNELASGSLVPILQEWPMEAFKVYGVYSSRRQLATNISVFLDFFHKRFTNQESLQSMMM

Sequences:

>Translated_303_residues
MPDLNGMMLFAAVVRAKGFSQAAREIGMPKSTISRKVAQLEEQLGVRLLQRDTRNLSLTQVGALFFQHCSSISDEIEAAK
ATVENTHNDVSGLLRIAIPVSFSQELIATLCSGFLRLYPNIELDVQFTDSDVGLVGEGYDIAIKYGPLQSSDLVARLLFE
RQPILVASPGYLKIHGTPATPQDLAEHSGILLGTSRSAPIWPLGKGARKTMVNFQRKVRVNSAVMVKQLVMDDFGIAMLS
NSVCKNELASGSLVPILQEWPMEAFKVYGVYSSRRQLATNISVFLDFFHKRFTNQESLQSMMM
>Mature_302_residues
PDLNGMMLFAAVVRAKGFSQAAREIGMPKSTISRKVAQLEEQLGVRLLQRDTRNLSLTQVGALFFQHCSSISDEIEAAKA
TVENTHNDVSGLLRIAIPVSFSQELIATLCSGFLRLYPNIELDVQFTDSDVGLVGEGYDIAIKYGPLQSSDLVARLLFER
QPILVASPGYLKIHGTPATPQDLAEHSGILLGTSRSAPIWPLGKGARKTMVNFQRKVRVNSAVMVKQLVMDDFGIAMLSN
SVCKNELASGSLVPILQEWPMEAFKVYGVYSSRRQLATNISVFLDFFHKRFTNQESLQSMMM

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=34.375, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=29.8305084745763, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=28.6195286195286, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=27.3972602739726, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=25.6055363321799, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=26.551724137931, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=25.3472222222222, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=26.7716535433071, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788706, Length=263, Percent_Identity=28.5171102661597, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI157672245, Length=170, Percent_Identity=31.1764705882353, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=25.5244755244755, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=21.4765100671141, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787879, Length=300, Percent_Identity=24.3333333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33460; Mature: 33329

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLNGMMLFAAVVRAKGFSQAAREIGMPKSTISRKVAQLEEQLGVRLLQRDTRNLSLTQ
CCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
VGALFFQHCSSISDEIEAAKATVENTHNDVSGLLRIAIPVSFSQELIATLCSGFLRLYPN
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCC
IELDVQFTDSDVGLVGEGYDIAIKYGPLQSSDLVARLLFERQPILVASPGYLKIHGTPAT
CEEEEEECCCCCCEEECCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCEEEEECCCCC
PQDLAEHSGILLGTSRSAPIWPLGKGARKTMVNFQRKVRVNSAVMVKQLVMDDFGIAMLS
HHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSVCKNELASGSLVPILQEWPMEAFKVYGVYSSRRQLATNISVFLDFFHKRFTNQESLQS
HHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MMM
HCC
>Mature Secondary Structure 
PDLNGMMLFAAVVRAKGFSQAAREIGMPKSTISRKVAQLEEQLGVRLLQRDTRNLSLTQ
CCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
VGALFFQHCSSISDEIEAAKATVENTHNDVSGLLRIAIPVSFSQELIATLCSGFLRLYPN
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCC
IELDVQFTDSDVGLVGEGYDIAIKYGPLQSSDLVARLLFERQPILVASPGYLKIHGTPAT
CEEEEEECCCCCCEEECCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCEEEEECCCCC
PQDLAEHSGILLGTSRSAPIWPLGKGARKTMVNFQRKVRVNSAVMVKQLVMDDFGIAMLS
HHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSVCKNELASGSLVPILQEWPMEAFKVYGVYSSRRQLATNISVFLDFFHKRFTNQESLQS
HHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
MMM
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]