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The map label for this gene is proS [H]

Identifier: 157825653

GI number: 157825653

Start: 521006

End: 522289

Strand: Direct

Name: proS [H]

Synonym: A1C_02875

Alternate gene names: 157825653

Gene position: 521006-522289 (Clockwise)

Preceding gene: 157825652

Following gene: 157825654

Centisome position: 42.32

GC content: 34.19

Gene sequence:

>1284_bases
ATGTTATTATCACAATATTTTTTACCTGTTTTAAAAGAAGAACCAAGTGAAGCTCAAGTAACTTCGCATAAATTAATGCT
TAGAAGCGGAATGATTAGGCAGCAGGCAGCAGGTATTTATACATGGCTTCCGCTTGGATTAAAGGTACTTAAGAATATCG
AGAACATAGTAAGTTTAAATATGAATAAAGCAGGCGCTTTAGAAGTTCTCATGCCTTGCATTCAACCGGCACATTTATGG
ATGGAATCGGGACGTTTTAATAATTACGGTAAAGAAATGCTGAAATTCCAAGATCGTCACGATAATACTTTACTATTCGG
TCCGACTAATGAAGATATGATTACGGATATTTTTCGTCATAATATTAAGTCATATAAGGATTTGCCTAAAAATCTTTATC
ATATCCAGTGGAAATTTCGTGATGAGATTAGACCACGTTTTGGCGTTATGAGAGGACGAGAGTTCCTTATGAAAGATGCT
TATTCTTTTGATATCAACGAAGAAAATGCCGTTAAGACCTATAATCAAATGTATAAGGCTTATATTAATACTTTCAGGGA
TTTAGGCGTGTTTGCTATCCCTGTTATTGCCGATAACGGTCCAATCGGCGGCAAGTTAAGCCATGAATTTCATATAATTG
CTGAGACCGGTGAAAGCACTATTTATTATGATAAAAGATTTAAGACATTAAAAGATAATCCTGACATCGATGTAGACGAA
ATTAAAAGCTGGTACGCAGCAGCTGAAGAAAAGCATGACATAAATAAATTACCTATATCTGAACAAGAAATAACCAGTAG
TAAAGGGATAGAAGTTGGGCATATTTTTTATATTGGATCAAAATATTCAGTTAATATGAAAGCTCTTATTAATGACGAAC
ATGGTAAACTAGCTCCTGTAGAAATGAGTTCATACGGCATAGGTATTTCAAGGCTAGTTGCAGCTATCATAGAAGCAAAT
TGCGATGAGAAAGGTATCATATGGCCTTTTAGCGTTGCTCCTTTTAAAGTTTCTCTAATTAATTTGAATATTCATGATAG
TAAATGTGTAGAGCTTGCGGCAAAAGCTTATAAAGATCTATCAGATAAAAATATAGAAGTGTTATATGACGATACTGAAG
CACGTCCAGGCAGTAAATTCGCAACGCATGACCTTATCGGCTCACCTCATCAAATTATAATTGGTCCTAAAAAAGCTGCA
AATAATATCGTTGAATTAAAAGACCGTAAAAGCGGAAATATAGAGGATATTGAGGTAGAAAATCTTATCAATTATATCAA
ATAA

Upstream 100 bases:

>100_bases
AGCATAATGAATACCATGCATTATACGGGTATATTTGCCGAGCAGCAGAAATATCGCCATGAGTTTTTGAATCTTACCAC
GAAAAGATAAGTGTATTTCT

Downstream 100 bases:

>100_bases
TATACCTATGATATTATCATAGGTATATTAAATATAATACGTAAAATATATGAATATATCTGCAACTGAGTTAAATAAAA
ATCCTGGAAAAATCATTGAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLLSQYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVSLNMNKAGALEVLMPCIQPAHLW
MESGRFNNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDA
YSFDINEENAVKTYNQMYKAYINTFRDLGVFAIPVIADNGPIGGKLSHEFHIIAETGESTIYYDKRFKTLKDNPDIDVDE
IKSWYAAAEEKHDINKLPISEQEITSSKGIEVGHIFYIGSKYSVNMKALINDEHGKLAPVEMSSYGIGISRLVAAIIEAN
CDEKGIIWPFSVAPFKVSLINLNIHDSKCVELAAKAYKDLSDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAA
NNIVELKDRKSGNIEDIEVENLINYIK

Sequences:

>Translated_427_residues
MLLSQYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVSLNMNKAGALEVLMPCIQPAHLW
MESGRFNNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDA
YSFDINEENAVKTYNQMYKAYINTFRDLGVFAIPVIADNGPIGGKLSHEFHIIAETGESTIYYDKRFKTLKDNPDIDVDE
IKSWYAAAEEKHDINKLPISEQEITSSKGIEVGHIFYIGSKYSVNMKALINDEHGKLAPVEMSSYGIGISRLVAAIIEAN
CDEKGIIWPFSVAPFKVSLINLNIHDSKCVELAAKAYKDLSDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAA
NNIVELKDRKSGNIEDIEVENLINYIK
>Mature_427_residues
MLLSQYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVSLNMNKAGALEVLMPCIQPAHLW
MESGRFNNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRHNIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDA
YSFDINEENAVKTYNQMYKAYINTFRDLGVFAIPVIADNGPIGGKLSHEFHIIAETGESTIYYDKRFKTLKDNPDIDVDE
IKSWYAAAEEKHDINKLPISEQEITSSKGIEVGHIFYIGSKYSVNMKALINDEHGKLAPVEMSSYGIGISRLVAAIIEAN
CDEKGIIWPFSVAPFKVSLINLNIHDSKCVELAAKAYKDLSDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAA
NNIVELKDRKSGNIEDIEVENLINYIK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=397, Percent_Identity=35.0125944584383, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1786392, Length=223, Percent_Identity=56.0538116591928, Blast_Score=278, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI115532348, Length=381, Percent_Identity=31.2335958005249, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI193203271, Length=94, Percent_Identity=30.8510638297872, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=202, Percent_Identity=41.5841584158416, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24656200, Length=392, Percent_Identity=31.1224489795918, Blast_Score=202, Evalue=5e-52,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 48650; Mature: 48650

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSQYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVSLN
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
MNKAGALEVLMPCIQPAHLWMESGRFNNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRH
CCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
NIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKA
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
YINTFRDLGVFAIPVIADNGPIGGKLSHEFHIIAETGESTIYYDKRFKTLKDNPDIDVDE
HHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECHHHHCCCCCCCCHHH
IKSWYAAAEEKHDINKLPISEQEITSSKGIEVGHIFYIGSKYSVNMKALINDEHGKLAPV
HHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEEEECCEEEEEEEEEEECCCCCEEEE
EMSSYGIGISRLVAAIIEANCDEKGIIWPFSVAPFKVSLINLNIHDSKCVELAAKAYKDL
ECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHCC
SDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAANNIVELKDRKSGNIEDIEVE
CCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEECCHHHHCCEEEECCCCCCCCCEEEHH
NLINYIK
HHHHHCC
>Mature Secondary Structure
MLLSQYFLPVLKEEPSEAQVTSHKLMLRSGMIRQQAAGIYTWLPLGLKVLKNIENIVSLN
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
MNKAGALEVLMPCIQPAHLWMESGRFNNYGKEMLKFQDRHDNTLLFGPTNEDMITDIFRH
CCCCCHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHH
NIKSYKDLPKNLYHIQWKFRDEIRPRFGVMRGREFLMKDAYSFDINEENAVKTYNQMYKA
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHH
YINTFRDLGVFAIPVIADNGPIGGKLSHEFHIIAETGESTIYYDKRFKTLKDNPDIDVDE
HHHHHHHCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECHHHHCCCCCCCCHHH
IKSWYAAAEEKHDINKLPISEQEITSSKGIEVGHIFYIGSKYSVNMKALINDEHGKLAPV
HHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEEEEECCEEEEEEEEEEECCCCCEEEE
EMSSYGIGISRLVAAIIEANCDEKGIIWPFSVAPFKVSLINLNIHDSKCVELAAKAYKDL
ECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHHHHCC
SDKNIEVLYDDTEARPGSKFATHDLIGSPHQIIIGPKKAANNIVELKDRKSGNIEDIEVE
CCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEECCHHHHCCEEEECCCCCCCCCEEEHH
NLINYIK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA