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The map label for this gene is eptB [H]

Identifier: 157825580

GI number: 157825580

Start: 449549

End: 451117

Strand: Reverse

Name: eptB [H]

Synonym: A1C_02465

Alternate gene names: 157825580

Gene position: 451117-449549 (Counterclockwise)

Preceding gene: 157825590

Following gene: 157825579

Centisome position: 36.64

GC content: 29.7

Gene sequence:

>1569_bases
ATGCTCAAAACTATTCAAAAACATATAGATATCAAATTAATTAAATTATCAGCGGTTTTAGCGTTTGTTTATTGTTTGTT
ATTTAATACCGCAATATTGATTTATAAATTTGATTATTATAAAGCAATAATCTTTAAAGGGATATTAGAATTATCAAAAG
ATTGTTGTTATATTTATATATTTTCTTTTATAACATTTTTCGGTCTTAGCGTACATAGATTAGTACTAAAAGCCGGCGTA
GGGTTTTTATTTATGACATCCGCTATTGCTAGTTATTACATTTATTTCTTCAAAATCAATCCTACTAAACAAGTAATAGG
TAGTTTTTTCTCAACAGATTTAAACGAAGTCTATGAATTAACCAGCATTAAGTTAGTAATATGGATAATTTTTTGCCTTT
TTACCTGTTTTTATATTCTCAAATCATTTGCAGCCGAAAATTCTAAATCATGCGTAACAAAATTATTATCTGCCGCTTGC
TTACTTATTTTTGTATATAATATTATTACTCCTTCATTTAAGATACTAAAAAATTATTTTCCCATTCAGTATTTACATAA
TAGCTACCTTAATTTTGCTGGAACTTTCGGTGATAAAAATTATGCATATATAGATATTAGCAAACAATATAATTTTATAG
ATAACTCCGACGCTGATATTATAGGAGTTTTAGTTATAGGCGAATCGGCAAGATTTGACCATTTCGGTATTAACGGTTAT
GAACGAGATACAACCCCTTACTTAAAAACCACCCCAAATCTAACTTCCTTCAAAGCTAAATCCTCTTCTAACCTTACTTA
TCTTTCCGTACCGTCGTTACTTTCACGCTATCCTGCAAGTCAAATTGAAAAGACTAGACAAGAAAACAGCTTTTTATCAA
TTTTAACAAATCTTGGCTTTCATACTACTTGGATTGGTACCCAAACTTTAATGAGGAGTTTTGCAAATTTTGACCTCAGT
AATATATATAATGACATTAATTTTACTATAGTACCGGGCGGCTCTGCCCTCTTTTCTTTAAATGATCATGATGAAAAAAT
ATTACCTTTCATAAAAGAAATAATAACAAACTCAGAGAAGCAATTTTTAGTAGTTCACACTTCCGGCAGCCATTGGAACT
ATAATGCAAGATATCCAAAAGAGTTTGAGTATTTTACCCCTACATGTCCTATTAAGGTTAAGGGAGATGCAAGCGATTGC
GATAAGCTTGAGTTAGTGAACAGTTATGATAATTCTATTTTATATACCGATTTTTTTTTATCTAATTTAATAGATTTGCT
TAAAGATAAAAATGCATTTTTATTATATGTATCCGATCACGGCGAGTCCCTCGGAGAAAATGGTTATTACGGTCACGGCG
GTCCACTACTTGCAGAACAAATAACCGTACCTCTTATAGTTTGGGTATCAGATGATTTTCAAACTAAATATCCTAAATCA
GTATCTTCAATTAAAAATTATGCTAATACCGAGATTAGCCATGATTATGTATTTCATTCAATCCTTGACTGTTTAAATAT
AGATTCTGACATAATAGATAAAAACTTAAGTATATGTAAATCTAGTTAA

Upstream 100 bases:

>100_bases
ATGCTTGAAGGTTTTTTATTATCATGGTAGTTATTAAGTCTACTTATATTATACTCGTTGAACTTCGTATCACTACTATT
TATTTCATATTACTTAATTT

Downstream 100 bases:

>100_bases
AAATTATTGCATTATTAAAAAATTTATATATAAATTGAGAACTCAAGAATTATATGGTTTATTATTATGTCTGATATACC
TAAACTAGACGACTATTATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MLKTIQKHIDIKLIKLSAVLAFVYCLLFNTAILIYKFDYYKAIIFKGILELSKDCCYIYIFSFITFFGLSVHRLVLKAGV
GFLFMTSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYELTSIKLVIWIIFCLFTCFYILKSFAAENSKSCVTKLLSAAC
LLIFVYNIITPSFKILKNYFPIQYLHNSYLNFAGTFGDKNYAYIDISKQYNFIDNSDADIIGVLVIGESARFDHFGINGY
ERDTTPYLKTTPNLTSFKAKSSSNLTYLSVPSLLSRYPASQIEKTRQENSFLSILTNLGFHTTWIGTQTLMRSFANFDLS
NIYNDINFTIVPGGSALFSLNDHDEKILPFIKEIITNSEKQFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDC
DKLELVNSYDNSILYTDFFLSNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQTKYPKS
VSSIKNYANTEISHDYVFHSILDCLNIDSDIIDKNLSICKSS

Sequences:

>Translated_522_residues
MLKTIQKHIDIKLIKLSAVLAFVYCLLFNTAILIYKFDYYKAIIFKGILELSKDCCYIYIFSFITFFGLSVHRLVLKAGV
GFLFMTSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYELTSIKLVIWIIFCLFTCFYILKSFAAENSKSCVTKLLSAAC
LLIFVYNIITPSFKILKNYFPIQYLHNSYLNFAGTFGDKNYAYIDISKQYNFIDNSDADIIGVLVIGESARFDHFGINGY
ERDTTPYLKTTPNLTSFKAKSSSNLTYLSVPSLLSRYPASQIEKTRQENSFLSILTNLGFHTTWIGTQTLMRSFANFDLS
NIYNDINFTIVPGGSALFSLNDHDEKILPFIKEIITNSEKQFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDC
DKLELVNSYDNSILYTDFFLSNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQTKYPKS
VSSIKNYANTEISHDYVFHSILDCLNIDSDIIDKNLSICKSS
>Mature_522_residues
MLKTIQKHIDIKLIKLSAVLAFVYCLLFNTAILIYKFDYYKAIIFKGILELSKDCCYIYIFSFITFFGLSVHRLVLKAGV
GFLFMTSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYELTSIKLVIWIIFCLFTCFYILKSFAAENSKSCVTKLLSAAC
LLIFVYNIITPSFKILKNYFPIQYLHNSYLNFAGTFGDKNYAYIDISKQYNFIDNSDADIIGVLVIGESARFDHFGINGY
ERDTTPYLKTTPNLTSFKAKSSSNLTYLSVPSLLSRYPASQIEKTRQENSFLSILTNLGFHTTWIGTQTLMRSFANFDLS
NIYNDINFTIVPGGSALFSLNDHDEKILPFIKEIITNSEKQFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDC
DKLELVNSYDNSILYTDFFLSNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQTKYPKS
VSSIKNYANTEISHDYVFHSILDCLNIDSDIIDKNLSICKSS

Specific function: Catalyzes the addition of a phosphoethanolamine (pEtN) moiety to the outer 3-deoxy-D-manno-octulosonic acid (KDO) residue of a KDO(2)-lipid A. Phosphatidylethanolamines with one unsaturated acyl group functions as pEtN donors and the reaction releases dia

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082286, Length=542, Percent_Identity=27.3062730627306, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082372, Length=325, Percent_Identity=30.4615384615385, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082223, Length=362, Percent_Identity=27.9005524861878, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1790392, Length=307, Percent_Identity=28.0130293159609, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787035, Length=275, Percent_Identity=25.8181818181818, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59530; Mature: 59530

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTIQKHIDIKLIKLSAVLAFVYCLLFNTAILIYKFDYYKAIIFKGILELSKDCCYIYI
CCCHHHHHCCEEEEHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCHHHHHH
FSFITFFGLSVHRLVLKAGVGFLFMTSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYEL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHCCHHHHHHH
TSIKLVIWIIFCLFTCFYILKSFAAENSKSCVTKLLSAACLLIFVYNIITPSFKILKNYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
PIQYLHNSYLNFAGTFGDKNYAYIDISKQYNFIDNSDADIIGVLVIGESARFDHFGINGY
CHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
ERDTTPYLKTTPNLTSFKAKSSSNLTYLSVPSLLSRYPASQIEKTRQENSFLSILTNLGF
CCCCCCCEEECCCCCEEECCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
HTTWIGTQTLMRSFANFDLSNIYNDINFTIVPGGSALFSLNDHDEKILPFIKEIITNSEK
CEEEHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCC
QFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDCDKLELVNSYDNSILYTDFFL
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEHHHHH
SNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQTKYPKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHH
VSSIKNYANTEISHDYVFHSILDCLNIDSDIIDKNLSICKSS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHCCC
>Mature Secondary Structure
MLKTIQKHIDIKLIKLSAVLAFVYCLLFNTAILIYKFDYYKAIIFKGILELSKDCCYIYI
CCCHHHHHCCEEEEHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCHHHHHH
FSFITFFGLSVHRLVLKAGVGFLFMTSAIASYYIYFFKINPTKQVIGSFFSTDLNEVYEL
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHCCHHHHHHH
TSIKLVIWIIFCLFTCFYILKSFAAENSKSCVTKLLSAACLLIFVYNIITPSFKILKNYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
PIQYLHNSYLNFAGTFGDKNYAYIDISKQYNFIDNSDADIIGVLVIGESARFDHFGINGY
CHHHHHHHHHCCEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCC
ERDTTPYLKTTPNLTSFKAKSSSNLTYLSVPSLLSRYPASQIEKTRQENSFLSILTNLGF
CCCCCCCEEECCCCCEEECCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
HTTWIGTQTLMRSFANFDLSNIYNDINFTIVPGGSALFSLNDHDEKILPFIKEIITNSEK
CEEEHHHHHHHHHHHCCCHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCC
QFLVVHTSGSHWNYNARYPKEFEYFTPTCPIKVKGDASDCDKLELVNSYDNSILYTDFFL
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEHHHHH
SNLIDLLKDKNAFLLYVSDHGESLGENGYYGHGGPLLAEQITVPLIVWVSDDFQTKYPKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCHH
VSSIKNYANTEISHDYVFHSILDCLNIDSDIIDKNLSICKSS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 7721701 [H]