Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 157825556

GI number: 157825556

Start: 427268

End: 428641

Strand: Reverse

Name: proP [H]

Synonym: A1C_02335

Alternate gene names: 157825556

Gene position: 428641-427268 (Counterclockwise)

Preceding gene: 157825557

Following gene: 157825554

Centisome position: 34.82

GC content: 30.93

Gene sequence:

>1374_bases
TTGACTTTTAACCAAAAAAGTGTAGCTTATCTTGAATCTGAATTATATATGAGTGTTATGTTAGGCTACGAAAAAGAACA
AAGAAGTTTAAATAAAGAACAAAAACAAGCTGTAGGATTACTTTCTATAGGTACATTTCTGGAGTATTTTGACCTTATGC
TTTATGTGCATATGGCTGTGCTTTTAAATAATTTATTTTTTCCGGAATATGATCAATTCACTTTTTCGCTGCTTACTGCC
TTTTCTTTCTGTTCTACCTATTTGCTAAGACCTATAGGAGCGTTAATGTTTGGGTATATAGGCGATCATTTTGGACGTAA
GATTGTAGTTGTTTTAACTACCCTATTAATGGCTATTACGTGTGTGATTATAGGTAGTATGCCAACATATGCACAAATAG
GTATAACAGCCTCTTGGACTCTTACTATATGTCGTATAATTCAAGGTATGTCGGCAACTGCAGAGGCACGAGGTGCAGAG
CTATACTTAACCGAAAATTCATCTCCTCCTATACAATATCCATTAGTAGCAATTATAACCGTATTTTCTGCCGTAGGCAC
GACGGTAGCACTAGGTATTGCATCAATTTTTACTAATCACAATATTTATCAGCATGAACCAAGTTGGCGTATAGCATTTT
TTGTTGGTGCAACCATTGCTTTTGTAGGTACTATTGCGAGGACTAGTTTAAAAGAAGCAGAGGCATTTTCAAATAAAAAA
AATAAATTAAAACTAAGACTTCAAGAAAATAATATTACGCTTGAAGAAATTGACAAAGATATTATAGATCAACAAGTTCC
AAACGCCACTTCTATTTGGTATTTCTTTATCCAATGTGCTAGACCTCCGTGTTTTTATTTCGTGTATATATATTGTGCTG
ATATATTAAAAAGAGAATTCGGATTTACTCCATATCAAATTATAAATCAAAATTTTTGGGTATCTATAATAGATTTACTC
GGTATTATAGGTCTTGCATTTCTTAGCTATAAAATCCATCCTTTAAAAATCCTAAAGGTAAAATTATATCTATTTTTTAC
CTCTCTCATTTTTTTCCCTATAATGTTAAATTATAACCCTACTCCTTTATATATTTTTATTTTTCAATGTTTAGCTGCAT
TATTCGTATTTGATCACGTACCGGCAGCTCCTATATTTTATAAATATTTTCCAGTTTTTAAGAGATTTACATATACTAGT
ATGCTAAGTGCAATAGCAAAATTATTTACTTATATTATAACCTCATTTGGTTTAGTTTATCTTACCAATTATCTAGGATA
TTGGGGATTATTTTTAATTTTCATTCCGGTAGGTACAGCTTTCTTCATGGGAGTAACTTATTTTGAGAAAATGGAAAAGA
ATCAAAATTTATAA

Upstream 100 bases:

>100_bases
CTTTCAGAGAAAAATAGATAATTTAAAAATTTTATGTTAGATCACTTTAGATAATCTAGATATTTTGTAACAATATTTAT
TAAAGATTCTTAATCCTTTA

Downstream 100 bases:

>100_bases
TTTTATTAAGAGTAGAATTTATATTAAACTTTAAATTATTTTTATAGCATTCAGTATATATAAGCTCTGAAACTGAAGGT
ATCTCAAGGATTTCGCTTAA

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTFNQKSVAYLESELYMSVMLGYEKEQRSLNKEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDQFTFSLLTA
FSFCSTYLLRPIGALMFGYIGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASWTLTICRIIQGMSATAEARGAE
LYLTENSSPPIQYPLVAIITVFSAVGTTVALGIASIFTNHNIYQHEPSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKK
NKLKLRLQENNITLEEIDKDIIDQQVPNATSIWYFFIQCARPPCFYFVYIYCADILKREFGFTPYQIINQNFWVSIIDLL
GIIGLAFLSYKIHPLKILKVKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTS
MLSAIAKLFTYIITSFGLVYLTNYLGYWGLFLIFIPVGTAFFMGVTYFEKMEKNQNL

Sequences:

>Translated_457_residues
MTFNQKSVAYLESELYMSVMLGYEKEQRSLNKEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDQFTFSLLTA
FSFCSTYLLRPIGALMFGYIGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASWTLTICRIIQGMSATAEARGAE
LYLTENSSPPIQYPLVAIITVFSAVGTTVALGIASIFTNHNIYQHEPSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKK
NKLKLRLQENNITLEEIDKDIIDQQVPNATSIWYFFIQCARPPCFYFVYIYCADILKREFGFTPYQIINQNFWVSIIDLL
GIIGLAFLSYKIHPLKILKVKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTS
MLSAIAKLFTYIITSFGLVYLTNYLGYWGLFLIFIPVGTAFFMGVTYFEKMEKNQNL
>Mature_456_residues
TFNQKSVAYLESELYMSVMLGYEKEQRSLNKEQKQAVGLLSIGTFLEYFDLMLYVHMAVLLNNLFFPEYDQFTFSLLTAF
SFCSTYLLRPIGALMFGYIGDHFGRKIVVVLTTLLMAITCVIIGSMPTYAQIGITASWTLTICRIIQGMSATAEARGAEL
YLTENSSPPIQYPLVAIITVFSAVGTTVALGIASIFTNHNIYQHEPSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKKN
KLKLRLQENNITLEEIDKDIIDQQVPNATSIWYFFIQCARPPCFYFVYIYCADILKREFGFTPYQIINQNFWVSIIDLLG
IIGLAFLSYKIHPLKILKVKLYLFFTSLIFFPIMLNYNPTPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTSM
LSAIAKLFTYIITSFGLVYLTNYLGYWGLFLIFIPVGTAFFMGVTYFEKMEKNQNL

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=259, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789941, Length=223, Percent_Identity=35.4260089686099, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788292, Length=221, Percent_Identity=26.2443438914027, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788942, Length=211, Percent_Identity=28.436018957346, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082231, Length=253, Percent_Identity=24.5059288537549, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 52350; Mature: 52219

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNQKSVAYLESELYMSVMLGYEKEQRSLNKEQKQAVGLLSIGTFLEYFDLMLYVHMAV
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNNLFFPEYDQFTFSLLTAFSFCSTYLLRPIGALMFGYIGDHFGRKIVVVLTTLLMAIT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CVIIGSMPTYAQIGITASWTLTICRIIQGMSATAEARGAELYLTENSSPPIQYPLVAIIT
HHHHCCCCCHHEECCCHHHHHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHH
VFSAVGTTVALGIASIFTNHNIYQHEPSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKK
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NKLKLRLQENNITLEEIDKDIIDQQVPNATSIWYFFIQCARPPCFYFVYIYCADILKREF
CEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GFTPYQIINQNFWVSIIDLLGIIGLAFLSYKIHPLKILKVKLYLFFTSLIFFPIMLNYNP
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTSMLSAIAKLFTYIITSFGLVY
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTNYLGYWGLFLIFIPVGTAFFMGVTYFEKMEKNQNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TFNQKSVAYLESELYMSVMLGYEKEQRSLNKEQKQAVGLLSIGTFLEYFDLMLYVHMAV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLNNLFFPEYDQFTFSLLTAFSFCSTYLLRPIGALMFGYIGDHFGRKIVVVLTTLLMAIT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CVIIGSMPTYAQIGITASWTLTICRIIQGMSATAEARGAELYLTENSSPPIQYPLVAIIT
HHHHCCCCCHHEECCCHHHHHHHHHHHCCCCCCHHCCCCEEEEECCCCCCCHHHHHHHHH
VFSAVGTTVALGIASIFTNHNIYQHEPSWRIAFFVGATIAFVGTIARTSLKEAEAFSNKK
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NKLKLRLQENNITLEEIDKDIIDQQVPNATSIWYFFIQCARPPCFYFVYIYCADILKREF
CEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
GFTPYQIINQNFWVSIIDLLGIIGLAFLSYKIHPLKILKVKLYLFFTSLIFFPIMLNYNP
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
TPLYIFIFQCLAALFVFDHVPAAPIFYKYFPVFKRFTYTSMLSAIAKLFTYIITSFGLVY
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTNYLGYWGLFLIFIPVGTAFFMGVTYFEKMEKNQNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]