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The map label for this gene is rpoH [H]

Identifier: 157825543

GI number: 157825543

Start: 414458

End: 415339

Strand: Reverse

Name: rpoH [H]

Synonym: A1C_02270

Alternate gene names: 157825543

Gene position: 415339-414458 (Counterclockwise)

Preceding gene: 157825545

Following gene: 157825537

Centisome position: 33.74

GC content: 32.54

Gene sequence:

>882_bases
ATGACGAATAATATTAATGCATTAGCAATCTCTAGCGAGTCAGGGTTTTATAACTACTTACAGAAAATTAATAACATTCC
TTCACTTACGCAAGAAGAAGAGTTTTTATTAGCGAAATCCTATTTAGAAGAGAATGATTTACAAGCTGCACATAAATTAG
TCACGAGTCATCTTAAGCTTGTGGCAAAAATTGCAAGCGGCTATAGGACTTACGGCATTCCTATTACCGAATTGGTTTCG
GAAGGTAATATAGGTTTAATGCAGGCAGTAAAAAAATTCAATCCCGAACTTGGTTTCCGCCTGTCAACATATGCGATGTG
GTGGATTAAAGCAGCCATACAAGAATATATATTAAAATCTTGGTCATTAGTAAAGATGGGAACAACGGCTGCACAAAAAA
AATTATTTTTTAGTCTTAATAAAGTTAAGCATAAAATTACTAATTTATACTCAAGGGCTATTACTACCGATGATTTTGCA
AAAATAGCAGATGAATTAGGCGTTTCGGTTAATGAAGTGTCCGAGATGAATACTAGAATTTCAGGTCCTGATTTATCATT
AAATAACTCTATAAATAGTGATGATGCAGCATCAGGAGAACTGATAGAACTATTACCAGAAACACGCCCTACTCCAGAAG
CTATGGCTATTAATAAGCAAAATTTTACCAGTAAAAGGAAGTTATTAGCTAATGCTATGAAGATTTTAAACGATAGGGAA
CTTCGTATTTTAACAGAGCGTAAATTAACGGATACTCCTACAACTTTAGATATTTTAAGCAATGAGTATAATATTTCTAA
GGAACGTATTAGACAAATAGAAAATACTGCTTTTGAAAAAATAAAGAAGTTTATTTTAAACCATAGTAGAGAAGTAGCTT
AA

Upstream 100 bases:

>100_bases
GGATTTTTTAGACATTATGTGATTTTATTTAAAAATACTCTAGACAAAGTTTATTTTAGATCATATATGTGTTAGTATAA
AGAAAAATAGTAAGGTTGGT

Downstream 100 bases:

>100_bases
GCCTTATTGGATTGTAATCCAGTGTCTTGATATGCCATTGGGCTGCATAGATCGAAAAACCTACTTTATGTCATCCCCGC
GGAAGCGGGAATGACATAAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTNNINALAISSESGFYNYLQKINNIPSLTQEEEFLLAKSYLEENDLQAAHKLVTSHLKLVAKIASGYRTYGIPITELVS
EGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKSWSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFA
KIADELGVSVNEVSEMNTRISGPDLSLNNSINSDDAASGELIELLPETRPTPEAMAINKQNFTSKRKLLANAMKILNDRE
LRILTERKLTDTPTTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREVA

Sequences:

>Translated_293_residues
MTNNINALAISSESGFYNYLQKINNIPSLTQEEEFLLAKSYLEENDLQAAHKLVTSHLKLVAKIASGYRTYGIPITELVS
EGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKSWSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFA
KIADELGVSVNEVSEMNTRISGPDLSLNNSINSDDAASGELIELLPETRPTPEAMAINKQNFTSKRKLLANAMKILNDRE
LRILTERKLTDTPTTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREVA
>Mature_292_residues
TNNINALAISSESGFYNYLQKINNIPSLTQEEEFLLAKSYLEENDLQAAHKLVTSHLKLVAKIASGYRTYGIPITELVSE
GNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKSWSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFAK
IADELGVSVNEVSEMNTRISGPDLSLNNSINSDDAASGELIELLPETRPTPEAMAINKQNFTSKRKLLANAMKILNDREL
RILTERKLTDTPTTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREVA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=36.9337979094077, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=29.9270072992701, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=27.7310924369748, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33123; Mature: 32992

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNINALAISSESGFYNYLQKINNIPSLTQEEEFLLAKSYLEENDLQAAHKLVTSHLKL
CCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIASGYRTYGIPITELVSEGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKS
HHHHHHCCEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFAKIADELGVSVNEVSEMNTRI
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCC
SGPDLSLNNSINSDDAASGELIELLPETRPTPEAMAINKQNFTSKRKLLANAMKILNDRE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCH
LRILTERKLTDTPTTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREVA
HEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNNINALAISSESGFYNYLQKINNIPSLTQEEEFLLAKSYLEENDLQAAHKLVTSHLKL
CCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIASGYRTYGIPITELVSEGNIGLMQAVKKFNPELGFRLSTYAMWWIKAAIQEYILKS
HHHHHHCCEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTAAQKKLFFSLNKVKHKITNLYSRAITTDDFAKIADELGVSVNEVSEMNTRI
CCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCC
SGPDLSLNNSINSDDAASGELIELLPETRPTPEAMAINKQNFTSKRKLLANAMKILNDRE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCH
LRILTERKLTDTPTTLDILSNEYNISKERIRQIENTAFEKIKKFILNHSREVA
HEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]