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The map label for this gene is tolB [H]

Identifier: 157825542

GI number: 157825542

Start: 412988

End: 414322

Strand: Direct

Name: tolB [H]

Synonym: A1C_02265

Alternate gene names: 157825542

Gene position: 412988-414322 (Clockwise)

Preceding gene: 157825541

Following gene: 157825544

Centisome position: 33.55

GC content: 36.33

Gene sequence:

>1335_bases
GTGAGAAATATTATATATTTTATACTATTACTATTATTTAGCTTTAAAGGTTATGCACTTGAAACAATAAATATTGAGCA
TGGGCAAGCTGCTCCTACGCCTATAGCAGTTAATAAATTTAATATCGATAGTTCTGCTGATTACGTACTGGGTAATGATG
TGGTTAAAGTTATCTCTAATGATTTAAAGCTTTCGGGGGTATTCTGTCCTATTTCTTCTGCATCTTTTATAGAAGAGAGG
ACAGGAATAGAATATAAACCGCTTTTTGCTGCATGGCGTCAAATTAATGCTAGCCTTTTAGTAAATGGTGAAATTAAAAA
ACTAGAAAGCGGTAAGCTTAAAATTAGCTTTATATTATGGGATACATTGCTTGAAAAACAGCTTGCCGGCGAAATTCTTG
AAGTACCAGAAAATTTATGGCGAAGAGCAGCACATAAAATTGCTGATAAAATTTATGAAAAAATTACCGGTGATGCCGGT
TATTTTGACACCAAAATAGTGTATGTATCAGAAAGCACTTCTTTACCAAAAATAAAAAGAATTGCTTTGATGGATTATGA
CGGTGCTAATAATAAATATCTGACCAATGGAAGGTCACTAGTATTAACCCCAAGATTTGCTCGTTCAGCGGATAAGATTT
TTTATGTTTCATACGCAACTAAAAGAAGAGCTCTTGTTTATGAAAAGGATTTAAAAACAGGTAAAGAAAGCGTAGTAGGT
GACTTTTCTGGTATATCTTTTGCTCCTAGATTCTCACCGGACGGTAGAAAAGCCGTAATGTCAATAGCTAAAAACGGCTC
AACTCATATTTACGAAATTGACCTTGCTACTAAACGGCTGCATAAATTAACTGACGGATTCGGTATTAATACCTCTCCTA
GCTATTCACCGGACGGTAAAAAAATTGTATATAACTCCGATAGAAACGGTGTGCCTCAATTATATATCATGAATTCGGAC
GGAAGTGACGTTCAGCGTATTAGTTTCGGTGGAGGTTCTTACACTGCTCCTAGCTGGTCGCCTAGAGGAGACTATATAGC
ATTTACTAAGATTATTAGAGGAGCTGAAGGAAAAACTTTTAATATTGGAATTATGAAAGCATACCCCCAAGATGATGAGA
ATCGTGAAAGGATAATAACAAGCGGATATTTAGTCGAAAGCCCTTGTTGGTCACCTAACGGACGAGTCATTATGTTTGCT
AAAGGTTGGCCGTCTGGAGCTAAAGCTCCGGGGAAAAACAAAATTTTTGCAATTGATCTAACAGGTCATAATGAAAGAGA
AATTATAACACCGGAGGATGCTTCTGATCCTGAATGGTCAGGAGTGCTGAATTGA

Upstream 100 bases:

>100_bases
TGATTTATTCACGGGGTGACAACAAAAAATTTGGATTTCTGCTCCCGCAGGAATGATATATAATAACCATAACGGAATTT
GATAAAAAGGAGTATTTTGA

Downstream 100 bases:

>100_bases
TGTTACTTCTGTGAAAGCATTGTTACGTGGATCATTTTATGTCATTCCCGCTTCCGCGGGGATGACATAAAGTAGGTTTT
TCGATCTATGCAGCCCAATG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRNIIYFILLLLFSFKGYALETINIEHGQAAPTPIAVNKFNIDSSADYVLGNDVVKVISNDLKLSGVFCPISSASFIEER
TGIEYKPLFAAWRQINASLLVNGEIKKLESGKLKISFILWDTLLEKQLAGEILEVPENLWRRAAHKIADKIYEKITGDAG
YFDTKIVYVSESTSLPKIKRIALMDYDGANNKYLTNGRSLVLTPRFARSADKIFYVSYATKRRALVYEKDLKTGKESVVG
DFSGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGKKIVYNSDRNGVPQLYIMNSD
GSDVQRISFGGGSYTAPSWSPRGDYIAFTKIIRGAEGKTFNIGIMKAYPQDDENRERIITSGYLVESPCWSPNGRVIMFA
KGWPSGAKAPGKNKIFAIDLTGHNEREIITPEDASDPEWSGVLN

Sequences:

>Translated_444_residues
MRNIIYFILLLLFSFKGYALETINIEHGQAAPTPIAVNKFNIDSSADYVLGNDVVKVISNDLKLSGVFCPISSASFIEER
TGIEYKPLFAAWRQINASLLVNGEIKKLESGKLKISFILWDTLLEKQLAGEILEVPENLWRRAAHKIADKIYEKITGDAG
YFDTKIVYVSESTSLPKIKRIALMDYDGANNKYLTNGRSLVLTPRFARSADKIFYVSYATKRRALVYEKDLKTGKESVVG
DFSGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGKKIVYNSDRNGVPQLYIMNSD
GSDVQRISFGGGSYTAPSWSPRGDYIAFTKIIRGAEGKTFNIGIMKAYPQDDENRERIITSGYLVESPCWSPNGRVIMFA
KGWPSGAKAPGKNKIFAIDLTGHNEREIITPEDASDPEWSGVLN
>Mature_444_residues
MRNIIYFILLLLFSFKGYALETINIEHGQAAPTPIAVNKFNIDSSADYVLGNDVVKVISNDLKLSGVFCPISSASFIEER
TGIEYKPLFAAWRQINASLLVNGEIKKLESGKLKISFILWDTLLEKQLAGEILEVPENLWRRAAHKIADKIYEKITGDAG
YFDTKIVYVSESTSLPKIKRIALMDYDGANNKYLTNGRSLVLTPRFARSADKIFYVSYATKRRALVYEKDLKTGKESVVG
DFSGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGKKIVYNSDRNGVPQLYIMNSD
GSDVQRISFGGGSYTAPSWSPRGDYIAFTKIIRGAEGKTFNIGIMKAYPQDDENRERIITSGYLVESPCWSPNGRVIMFA
KGWPSGAKAPGKNKIFAIDLTGHNEREIITPEDASDPEWSGVLN

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=400, Percent_Identity=28, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 49354; Mature: 49354

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIIYFILLLLFSFKGYALETINIEHGQAAPTPIAVNKFNIDSSADYVLGNDVVKVISN
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCEEECHHHHHHHHC
DLKLSGVFCPISSASFIEERTGIEYKPLFAAWRQINASLLVNGEIKKLESGKLKISFILW
CCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEECCCCEEEEEEEH
DTLLEKQLAGEILEVPENLWRRAAHKIADKIYEKITGDAGYFDTKIVYVSESTSLPKIKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEE
IALMDYDGANNKYLTNGRSLVLTPRFARSADKIFYVSYATKRRALVYEKDLKTGKESVVG
EEEEECCCCCCCEEECCCEEEEECHHHCCCCEEEEEEECCCCCEEEEEHHHCCCCHHHCC
DFSGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGK
CCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCC
KIVYNSDRNGVPQLYIMNSDGSDVQRISFGGGSYTAPSWSPRGDYIAFTKIIRGAEGKTF
EEEECCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHCCCCCCEE
NIGIMKAYPQDDENRERIITSGYLVESPCWSPNGRVIMFAKGWPSGAKAPGKNKIFAIDL
EEEEEEECCCCCCCCCEEEEECEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEE
TGHNEREIITPEDASDPEWSGVLN
CCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNIIYFILLLLFSFKGYALETINIEHGQAAPTPIAVNKFNIDSSADYVLGNDVVKVISN
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCEEECHHHHHHHHC
DLKLSGVFCPISSASFIEERTGIEYKPLFAAWRQINASLLVNGEIKKLESGKLKISFILW
CCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCEEECCCCEEEEEEEH
DTLLEKQLAGEILEVPENLWRRAAHKIADKIYEKITGDAGYFDTKIVYVSESTSLPKIKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEE
IALMDYDGANNKYLTNGRSLVLTPRFARSADKIFYVSYATKRRALVYEKDLKTGKESVVG
EEEEECCCCCCCEEECCCEEEEECHHHCCCCEEEEEEECCCCCEEEEEHHHCCCCHHHCC
DFSGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGK
CCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCC
KIVYNSDRNGVPQLYIMNSDGSDVQRISFGGGSYTAPSWSPRGDYIAFTKIIRGAEGKTF
EEEECCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEHHHCCCCCCEE
NIGIMKAYPQDDENRERIITSGYLVESPCWSPNGRVIMFAKGWPSGAKAPGKNKIFAIDL
EEEEEEECCCCCCCCCEEEEECEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEE
TGHNEREIITPEDASDPEWSGVLN
CCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA