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The map label for this gene is prfB [H]

Identifier: 157825493

GI number: 157825493

Start: 368895

End: 369752

Strand: Reverse

Name: prfB [H]

Synonym: A1C_01970

Alternate gene names: 157825493

Gene position: 369752-368895 (Counterclockwise)

Preceding gene: 157825496

Following gene: 157825478

Centisome position: 30.04

GC content: 41.14

Gene sequence:

>858_bases
ATGGCTGCATCCGAAAACGATTTAGAAACACTTTCACAAATTGAACAGGACTTAAAAAATTTAAGCATCCTTGCTGCCAA
ATTTGAAACCGAATGTTTATTTTCAGGTGAAGCGGACGGTAATAACTGTTTTTTAGAGATTAATGCTGGCGCAGGAGGAA
CTGAAAGCCACGACTGGGCATCTATTATGATGCGTATGTATTTGCGTTTTGCAGAAAGACTCGGCTTTAAAACCGAAATA
ATCAATATGCTTAACGGCGAAGAAGTAGGCATTAAATCATGCACGATTAGGATAATAGGCAAGAGGGCTTACGGCTGGTT
TAAAACCGAAGCAGGAGTTCATAGGCTGGTACGTATTTCCCCGTTTAATGCTGCAGGTAAAAGAATGACTAGCTTTGCAA
GTAGCTGGGTATACCCTGCAATTGACGATAATATAGCAATTACTATTGAGGATAAAGATTTAAGGATCGATACTTTTAGA
GCATCGGGAGCAGGCGGACAGCACGTTAATACTACTGATTCTGCCGTACGTATCACCCATATACCGACCGGTACGGTAAC
ACAATGTCAAAGCGATAGATCGCAACATAAAAATAAAGCTCAAGCTATGAAGATGCTTCAAGCAAAACTCTATGAGCTTG
AAATGCAAAAACGCACAGACAGCGTTAACGAGCAAAATGCTGCTAAAACTGATAGTAGCTGGGGATATCAAATTAGATCA
TACATCCTACATCCTTACCAAATGGTGAAAGATTTACGCACCGATTACGAAACTTCCGACACTAAAGGAGTGCTTGACGG
TGACCTTGAAGAATTCGTCTCAGCAAACCTTGCTATGAATGCGGGCGGTAAGAGTTAG

Upstream 100 bases:

>100_bases
CGCACAAACATTACTGCGAGAAAAAAGTAATCTAGAAGAAAAACTAAATGCTTTTAATAAACTCAAATCCAATTTAAAAG
ATGCTTTAGAACTTGAAGAA

Downstream 100 bases:

>100_bases
TGACAATCAATGAGCAATTAATGAATCATATACTGACTTAATTAAAAATTTTAAACAAGAAATCAGTAAAGCTAGTATTA
GAGCCCATTTAGCAGTTAAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAASENDLETLSQIEQDLKNLSILAAKFETECLFSGEADGNNCFLEINAGAGGTESHDWASIMMRMYLRFAERLGFKTEI
INMLNGEEVGIKSCTIRIIGKRAYGWFKTEAGVHRLVRISPFNAAGKRMTSFASSWVYPAIDDNIAITIEDKDLRIDTFR
ASGAGGQHVNTTDSAVRITHIPTGTVTQCQSDRSQHKNKAQAMKMLQAKLYELEMQKRTDSVNEQNAAKTDSSWGYQIRS
YILHPYQMVKDLRTDYETSDTKGVLDGDLEEFVSANLAMNAGGKS

Sequences:

>Translated_285_residues
MAASENDLETLSQIEQDLKNLSILAAKFETECLFSGEADGNNCFLEINAGAGGTESHDWASIMMRMYLRFAERLGFKTEI
INMLNGEEVGIKSCTIRIIGKRAYGWFKTEAGVHRLVRISPFNAAGKRMTSFASSWVYPAIDDNIAITIEDKDLRIDTFR
ASGAGGQHVNTTDSAVRITHIPTGTVTQCQSDRSQHKNKAQAMKMLQAKLYELEMQKRTDSVNEQNAAKTDSSWGYQIRS
YILHPYQMVKDLRTDYETSDTKGVLDGDLEEFVSANLAMNAGGKS
>Mature_284_residues
AASENDLETLSQIEQDLKNLSILAAKFETECLFSGEADGNNCFLEINAGAGGTESHDWASIMMRMYLRFAERLGFKTEII
NMLNGEEVGIKSCTIRIIGKRAYGWFKTEAGVHRLVRISPFNAAGKRMTSFASSWVYPAIDDNIAITIEDKDLRIDTFRA
SGAGGQHVNTTDSAVRITHIPTGTVTQCQSDRSQHKNKAQAMKMLQAKLYELEMQKRTDSVNEQNAAKTDSSWGYQIRSY
ILHPYQMVKDLRTDYETSDTKGVLDGDLEEFVSANLAMNAGGKS

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=39.3501805054152, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI34577120, Length=238, Percent_Identity=38.655462184874, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI166795305, Length=179, Percent_Identity=40.2234636871508, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI2367172, Length=277, Percent_Identity=57.7617328519856, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1787462, Length=180, Percent_Identity=48.3333333333333, Blast_Score=174, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=255, Percent_Identity=31.3725490196078, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=243, Percent_Identity=37.037037037037, Blast_Score=135, Evalue=7e-33,
Organism=Drosophila melanogaster, GI19921226, Length=210, Percent_Identity=40.4761904761905, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 31704; Mature: 31573

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASENDLETLSQIEQDLKNLSILAAKFETECLFSGEADGNNCFLEINAGAGGTESHDWA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCCCCHHHH
SIMMRMYLRFAERLGFKTEIINMLNGEEVGIKSCTIRIIGKRAYGWFKTEAGVHRLVRIS
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEHHHHHHEEEEEEC
PFNAAGKRMTSFASSWVYPAIDDNIAITIEDKDLRIDTFRASGAGGQHVNTTDSAVRITH
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEE
IPTGTVTQCQSDRSQHKNKAQAMKMLQAKLYELEMQKRTDSVNEQNAAKTDSSWGYQIRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHH
YILHPYQMVKDLRTDYETSDTKGVLDGDLEEFVSANLAMNAGGKS
HHHCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCC
>Mature Secondary Structure 
AASENDLETLSQIEQDLKNLSILAAKFETECLFSGEADGNNCFLEINAGAGGTESHDWA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCCCCHHHH
SIMMRMYLRFAERLGFKTEIINMLNGEEVGIKSCTIRIIGKRAYGWFKTEAGVHRLVRIS
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEHHHHHHEEEEEEC
PFNAAGKRMTSFASSWVYPAIDDNIAITIEDKDLRIDTFRASGAGGQHVNTTDSAVRITH
CCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEEEEE
IPTGTVTQCQSDRSQHKNKAQAMKMLQAKLYELEMQKRTDSVNEQNAAKTDSSWGYQIRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHH
YILHPYQMVKDLRTDYETSDTKGVLDGDLEEFVSANLAMNAGGKS
HHHCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA