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The map label for this gene is yafC [C]

Identifier: 157373096

GI number: 157373096

Start: 5385882

End: 5386775

Strand: Reverse

Name: yafC [C]

Synonym: Spro_4864

Alternate gene names: 157373096

Gene position: 5386775-5385882 (Counterclockwise)

Preceding gene: 157373103

Following gene: 157373094

Centisome position: 98.86

GC content: 57.05

Gene sequence:

>894_bases
ATGGACAGTCTCAGCGGCTTATTGGCTTTTGTGCGCGCTGCCGAGCTACACAGTTTTGTCGCCGCAGGTGAACGGCTGGG
TATATCTGCTTCCGCCGTAGGGAAAAGCGTAGCCAGGCTGGAGGAATCATTGGGTGTCAGGCTGTTTAACCGCAGCACCC
GGCGCATCAATTTAACCGAGGAAGGCGCACTGTTTTTTGAACGTTGCCAACGCATCGTCAGCGAAATTGAAAATGCAGAA
GCCGAAATGGCGAGGATCTCTGATGCGCCGCGCGGCAAGCTACGCGTGAGCATGCCGGCTATTGGCTATCGAATGTTGGT
CCCGCTGCTGGCTGATTTCAGTGAGCTTTATCCCGAGGTTGAGCTGGATGTGGATTTTAATGACCGGATGGTGGATCTGA
TCGCCGAAGGTATTGACGTGGCGGTGCGCAGCGGTGATCTGGCGGACTCCAGGCTGATGGCTCGGCGGCTGGGGCCGTTC
CGGTTGATACTGGCGGGAGCGCCGGAGTATTTCCAGCGGCGGGGCATGCCGCAGGTACCGGAAGATTTACTCAGCCATGC
CTGCTTACGCTATCGCTATCCAGGGGGGCAGCAACTGCAGGAGTGGACATTATTTGCTGATGGGGAACCGGTTTCCCTTC
GAGTCCCGGTGACGCTGAGTTGCAACAGTATCGAAGCGTTGATCCACGCCACCCAAGAAGGGTTGGGTATCGCCTACCTG
CCGGATTTTACGCTGTGTCAGGCTGCTAACGCCGGACGGCTGACGGCGGTGTTGGATCAATACACGCAGGAGCAGGGCTT
TTTCTCTGCCCTGTGGCCATCAAGCCGCCATATGCTGCCAAAGGTGAGGGTCTTTGTAGACTACCTGGCGCAGCATATGT
TGCCCTTGAGATGA

Upstream 100 bases:

>100_bases
AGTATTGTTGGCTGGATTGGATGATAAAGAGGATTTAATTGGCCAAAAATGGGGCTTTACTCCACTCTTTAAGGACAATA
ACTCTCTAATTGGAGCGCAG

Downstream 100 bases:

>100_bases
ACCATTATTAATGATCCGGGTGCTGGTAATTAAATACCGGCAGGCCCAGACGAAAGCGCAGGGCGAGCAAGCGGGCGCTG
AGGCCGACGACCAGCGTGAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MDSLSGLLAFVRAAELHSFVAAGERLGISASAVGKSVARLEESLGVRLFNRSTRRINLTEEGALFFERCQRIVSEIENAE
AEMARISDAPRGKLRVSMPAIGYRMLVPLLADFSELYPEVELDVDFNDRMVDLIAEGIDVAVRSGDLADSRLMARRLGPF
RLILAGAPEYFQRRGMPQVPEDLLSHACLRYRYPGGQQLQEWTLFADGEPVSLRVPVTLSCNSIEALIHATQEGLGIAYL
PDFTLCQAANAGRLTAVLDQYTQEQGFFSALWPSSRHMLPKVRVFVDYLAQHMLPLR

Sequences:

>Translated_297_residues
MDSLSGLLAFVRAAELHSFVAAGERLGISASAVGKSVARLEESLGVRLFNRSTRRINLTEEGALFFERCQRIVSEIENAE
AEMARISDAPRGKLRVSMPAIGYRMLVPLLADFSELYPEVELDVDFNDRMVDLIAEGIDVAVRSGDLADSRLMARRLGPF
RLILAGAPEYFQRRGMPQVPEDLLSHACLRYRYPGGQQLQEWTLFADGEPVSLRVPVTLSCNSIEALIHATQEGLGIAYL
PDFTLCQAANAGRLTAVLDQYTQEQGFFSALWPSSRHMLPKVRVFVDYLAQHMLPLR
>Mature_297_residues
MDSLSGLLAFVRAAELHSFVAAGERLGISASAVGKSVARLEESLGVRLFNRSTRRINLTEEGALFFERCQRIVSEIENAE
AEMARISDAPRGKLRVSMPAIGYRMLVPLLADFSELYPEVELDVDFNDRMVDLIAEGIDVAVRSGDLADSRLMARRLGPF
RLILAGAPEYFQRRGMPQVPEDLLSHACLRYRYPGGQQLQEWTLFADGEPVSLRVPVTLSCNSIEALIHATQEGLGIAYL
PDFTLCQAANAGRLTAVLDQYTQEQGFFSALWPSSRHMLPKVRVFVDYLAQHMLPLR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=38.013698630137, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=32.5259515570934, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI145693193, Length=304, Percent_Identity=32.2368421052632, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=30, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=31.3131313131313, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=32.9931972789116, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1789440, Length=275, Percent_Identity=30.1818181818182, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=27.7397260273973, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=27.1777003484321, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=24.6575342465753, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786508, Length=172, Percent_Identity=28.4883720930233, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=32.1167883211679, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788748, Length=146, Percent_Identity=34.9315068493151, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=153, Percent_Identity=25.4901960784314, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=23.7931034482759, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=249, Percent_Identity=24.8995983935743, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33058; Mature: 33058

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLSGLLAFVRAAELHSFVAAGERLGISASAVGKSVARLEESLGVRLFNRSTRRINLTE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEECCH
EGALFFERCQRIVSEIENAEAEMARISDAPRGKLRVSMPAIGYRMLVPLLADFSELYPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCE
ELDVDFNDRMVDLIAEGIDVAVRSGDLADSRLMARRLGPFRLILAGAPEYFQRRGMPQVP
EEECCCCHHHHHHHHHCHHEEEECCCCHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCH
EDLLSHACLRYRYPGGQQLQEWTLFADGEPVSLRVPVTLSCNSIEALIHATQEGLGIAYL
HHHHHHHHHHCCCCCCCCHHHEEEEECCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEC
PDFTLCQAANAGRLTAVLDQYTQEQGFFSALWPSSRHMLPKVRVFVDYLAQHMLPLR
CCCHHHHCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDSLSGLLAFVRAAELHSFVAAGERLGISASAVGKSVARLEESLGVRLFNRSTRRINLTE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEEEECCCCCEEECCH
EGALFFERCQRIVSEIENAEAEMARISDAPRGKLRVSMPAIGYRMLVPLLADFSELYPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCE
ELDVDFNDRMVDLIAEGIDVAVRSGDLADSRLMARRLGPFRLILAGAPEYFQRRGMPQVP
EEECCCCHHHHHHHHHCHHEEEECCCCHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCH
EDLLSHACLRYRYPGGQQLQEWTLFADGEPVSLRVPVTLSCNSIEALIHATQEGLGIAYL
HHHHHHHHHHCCCCCCCCHHHEEEEECCCCEEEEEEEEECCCHHHHHHHHHHCCCCEEEC
PDFTLCQAANAGRLTAVLDQYTQEQGFFSALWPSSRHMLPKVRVFVDYLAQHMLPLR
CCCHHHHCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]