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The map label for this gene is lcfB [H]

Identifier: 157373091

GI number: 157373091

Start: 5380578

End: 5382116

Strand: Reverse

Name: lcfB [H]

Synonym: Spro_4859

Alternate gene names: 157373091

Gene position: 5382116-5380578 (Counterclockwise)

Preceding gene: 157373092

Following gene: 157373090

Centisome position: 98.78

GC content: 48.02

Gene sequence:

>1539_bases
ATGGAAACAATTCATCTTGGTAAAAGTAATTTTTCATCTATATCTGAGTTGATCTCGATTCATGCTATCGAACGTGCTGA
CACTGTCGCTTTACGGTACAGGGATACAGTTGTTACTTATAAAGAATTGCAGCGTTATTCTGAACAGATCTTGGCAGCGA
TACAGTATAATGATATCCCCCCGCGCACGGCTATTGCTATTGCTTCGTCCAATCCATTGCTATCCACTTTGGCCCTTCTC
GGCGTTATTAGTGCCGGTTGTGTCGCTGTACCCGTCGCTTTATCGTCGTCTCCCGAACAAATAGCATCGGTAATTGCAGA
TGCATGTATTCCTTTGGTTTTTTTGGATAGCGAGTTTCTTGAGGGTTTGCCATCGATTGGTGCCAGGGTCGCTAATCTTG
ACGAATTCGATAGCTGGTTAGCACCTGAAGGGTTTGCTGCCACGCATGTTGAGCTGTCGCCGGCAGACCCCTTTAACATT
ATCTATTCATCAGGGACCACGGGTACGCCAAAGGGAATTATCCATACGCATGGTATGCGTTGGAGTCAAATAGCGGCATA
TGGGCAGATTGTTCCGCAATGTGACTCTGCCGTCACTTTGATCGCCACGCCGATCTATTCCAATACGACAATAGTCAGTT
TGTTGCCGTCGCTTGCCTACGGAGGGACTGCAGTGCTGATGGGTAAATTCGATGCTTATGAGTATTTAGTCGAAGCGCAG
AATTCACGGGCTACCCACACCTTGATGGTCCCTGTGCAGTACCAACGTATTATGGCTTTAACAAAATTCGACGATTTTGA
TCTTAGTAGTTTCATTTTCAAATCATGCACGGGCGCACCTTTTTCTCCCGAGTTGAAGGCCGATGTAATCCGTCGCTGGC
CAGGAGCCTTGCTCGAAATTTACGGGATGACAGAAGGAGGGGGGACCTGTGTTCTCAGGGCAAATGAATGTCCTGAGAAA
CTCCACACGGTAGGTCAGCCTGTGCCTGGCTGTGAAATTCGCTTGATTGATGCACAAGGGAATGAAGTCAATTCTGGCGA
GATCGGTGAGATCGTGGGGCGCTCAAATATGATGATGACTGGTTATCATGGGCGCCCGGACACGACCCGTGAAGCCACCT
GGATTGATGCTAATGGCAATAGATATATACGGCACGGGGACCTTGGGCGTTTCGATGAAGAGGGTTTTTTGACACTCCTT
GGGCGCATTAAAGACATGATCATTAGCGGTGGTTTTAATATATATCCATCAGATATAGAAGCAGTTTTGCGGTTACATCC
TTCCATTGCAGATTGCGCGGTTATAGGTATTCCCTCGGTTACCTGGGGGGAAACTCCCTTCGCCTTCTACGTCCCCAAAA
ACGACTCGCTCAGCCCTAACGAAGTCATCACTTGGGTTAATCAGCGTATGGGTAAGACACAGCGTTTATCCGACGCACAG
GCCATTGCTCAATTGCCACTTAGCGCTACGGGAAAAGTACTGAAACGTGAACTGCGCGCGCTCTTTCTGACCGAACACCC
TACCCCATTTGCATTATAA

Upstream 100 bases:

>100_bases
TTTATGGTTTCATTTTTGAAACGACTGGTTTCATTGCGGTGGCTAGTCAAAATTTAAATGCATGTCGATAGTGAGTGAAT
TGATAATGAGGATTCTTTTC

Downstream 100 bases:

>100_bases
GGAACTGAATTATGAAACCCGACCTGCAGGATCGTGTTGCGATTATTACCGGAGCCGGAGGCGGGCTTGGCCGAGCACAC
GCACATCTGCTAGCCCGTTA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
METIHLGKSNFSSISELISIHAIERADTVALRYRDTVVTYKELQRYSEQILAAIQYNDIPPRTAIAIASSNPLLSTLALL
GVISAGCVAVPVALSSSPEQIASVIADACIPLVFLDSEFLEGLPSIGARVANLDEFDSWLAPEGFAATHVELSPADPFNI
IYSSGTTGTPKGIIHTHGMRWSQIAAYGQIVPQCDSAVTLIATPIYSNTTIVSLLPSLAYGGTAVLMGKFDAYEYLVEAQ
NSRATHTLMVPVQYQRIMALTKFDDFDLSSFIFKSCTGAPFSPELKADVIRRWPGALLEIYGMTEGGGTCVLRANECPEK
LHTVGQPVPGCEIRLIDAQGNEVNSGEIGEIVGRSNMMMTGYHGRPDTTREATWIDANGNRYIRHGDLGRFDEEGFLTLL
GRIKDMIISGGFNIYPSDIEAVLRLHPSIADCAVIGIPSVTWGETPFAFYVPKNDSLSPNEVITWVNQRMGKTQRLSDAQ
AIAQLPLSATGKVLKRELRALFLTEHPTPFAL

Sequences:

>Translated_512_residues
METIHLGKSNFSSISELISIHAIERADTVALRYRDTVVTYKELQRYSEQILAAIQYNDIPPRTAIAIASSNPLLSTLALL
GVISAGCVAVPVALSSSPEQIASVIADACIPLVFLDSEFLEGLPSIGARVANLDEFDSWLAPEGFAATHVELSPADPFNI
IYSSGTTGTPKGIIHTHGMRWSQIAAYGQIVPQCDSAVTLIATPIYSNTTIVSLLPSLAYGGTAVLMGKFDAYEYLVEAQ
NSRATHTLMVPVQYQRIMALTKFDDFDLSSFIFKSCTGAPFSPELKADVIRRWPGALLEIYGMTEGGGTCVLRANECPEK
LHTVGQPVPGCEIRLIDAQGNEVNSGEIGEIVGRSNMMMTGYHGRPDTTREATWIDANGNRYIRHGDLGRFDEEGFLTLL
GRIKDMIISGGFNIYPSDIEAVLRLHPSIADCAVIGIPSVTWGETPFAFYVPKNDSLSPNEVITWVNQRMGKTQRLSDAQ
AIAQLPLSATGKVLKRELRALFLTEHPTPFAL
>Mature_512_residues
METIHLGKSNFSSISELISIHAIERADTVALRYRDTVVTYKELQRYSEQILAAIQYNDIPPRTAIAIASSNPLLSTLALL
GVISAGCVAVPVALSSSPEQIASVIADACIPLVFLDSEFLEGLPSIGARVANLDEFDSWLAPEGFAATHVELSPADPFNI
IYSSGTTGTPKGIIHTHGMRWSQIAAYGQIVPQCDSAVTLIATPIYSNTTIVSLLPSLAYGGTAVLMGKFDAYEYLVEAQ
NSRATHTLMVPVQYQRIMALTKFDDFDLSSFIFKSCTGAPFSPELKADVIRRWPGALLEIYGMTEGGGTCVLRANECPEK
LHTVGQPVPGCEIRLIDAQGNEVNSGEIGEIVGRSNMMMTGYHGRPDTTREATWIDANGNRYIRHGDLGRFDEEGFLTLL
GRIKDMIISGGFNIYPSDIEAVLRLHPSIADCAVIGIPSVTWGETPFAFYVPKNDSLSPNEVITWVNQRMGKTQRLSDAQ
AIAQLPLSATGKVLKRELRALFLTEHPTPFAL

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=427, Percent_Identity=25.9953161592506, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI187761345, Length=557, Percent_Identity=25.673249551167, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI187761343, Length=557, Percent_Identity=25.673249551167, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI42544132, Length=366, Percent_Identity=25.9562841530055, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157311624, Length=460, Percent_Identity=24.3478260869565, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157311622, Length=460, Percent_Identity=24.3478260869565, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI58082049, Length=462, Percent_Identity=24.4588744588745, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI115511026, Length=465, Percent_Identity=25.8064516129032, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI38505220, Length=368, Percent_Identity=24.7282608695652, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI122937307, Length=375, Percent_Identity=26.1333333333333, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI13375727, Length=376, Percent_Identity=25.2659574468085, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI27477105, Length=140, Percent_Identity=35.7142857142857, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI8923896, Length=381, Percent_Identity=24.9343832020997, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI253970489, Length=381, Percent_Identity=24.9343832020997, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI195947378, Length=296, Percent_Identity=22.972972972973, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=26.865671641791, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI145693145, Length=346, Percent_Identity=29.4797687861272, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786810, Length=514, Percent_Identity=27.6264591439689, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI221142682, Length=356, Percent_Identity=26.685393258427, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1790505, Length=548, Percent_Identity=23.9051094890511, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786801, Length=444, Percent_Identity=24.0990990990991, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788595, Length=460, Percent_Identity=21.304347826087, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=380, Percent_Identity=28.6842105263158, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17559526, Length=353, Percent_Identity=30.5949008498584, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538037, Length=526, Percent_Identity=25.0950570342205, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17558820, Length=361, Percent_Identity=31.3019390581717, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=478, Percent_Identity=27.8242677824268, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=525, Percent_Identity=24.1904761904762, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71985884, Length=366, Percent_Identity=28.1420765027322, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=362, Percent_Identity=27.0718232044199, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=371, Percent_Identity=25.0673854447439, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=371, Percent_Identity=25.0673854447439, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=371, Percent_Identity=25.0673854447439, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=345, Percent_Identity=26.0869565217391, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17564090, Length=496, Percent_Identity=24.1935483870968, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=516, Percent_Identity=24.031007751938, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323182, Length=568, Percent_Identity=22.3591549295775, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21355181, Length=479, Percent_Identity=26.0960334029228, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24581924, Length=501, Percent_Identity=24.3512974051896, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21356947, Length=367, Percent_Identity=26.4305177111717, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648253, Length=418, Percent_Identity=25.8373205741627, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648255, Length=418, Percent_Identity=25.8373205741627, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18859661, Length=467, Percent_Identity=25.0535331905782, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653035, Length=206, Percent_Identity=31.5533980582524, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281365686, Length=362, Percent_Identity=25.9668508287293, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356441, Length=495, Percent_Identity=23.030303030303, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648260, Length=349, Percent_Identity=25.5014326647564, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161076582, Length=212, Percent_Identity=31.1320754716981, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358303, Length=349, Percent_Identity=24.3553008595989, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648257, Length=355, Percent_Identity=25.9154929577465, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24656500, Length=367, Percent_Identity=25.0681198910082, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI62472339, Length=375, Percent_Identity=25.6, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24667955, Length=379, Percent_Identity=25.3298153034301, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24667959, Length=375, Percent_Identity=25.6, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922652, Length=363, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355225, Length=374, Percent_Identity=22.4598930481283, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20130357, Length=361, Percent_Identity=24.3767313019391, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921316, Length=161, Percent_Identity=34.1614906832298, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221329755, Length=363, Percent_Identity=25.3443526170799, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 55669; Mature: 55669

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIHLGKSNFSSISELISIHAIERADTVALRYRDTVVTYKELQRYSEQILAAIQYNDIP
CCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
PRTAIAIASSNPLLSTLALLGVISAGCVAVPVALSSSPEQIASVIADACIPLVFLDSEFL
CCEEEEEECCCHHHHHHHHHHHHHCCHHEEEEECCCCHHHHHHHHHHHHHHEEEECCHHH
EGLPSIGARVANLDEFDSWLAPEGFAATHVELSPADPFNIIYSSGTTGTPKGIIHTHGMR
CCCHHHHHHHCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCEEEECCCC
WSQIAAYGQIVPQCDSAVTLIATPIYSNTTIVSLLPSLAYGGTAVLMGKFDAYEYLVEAQ
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCC
NSRATHTLMVPVQYQRIMALTKFDDFDLSSFIFKSCTGAPFSPELKADVIRRWPGALLEI
CCCCEEEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEE
YGMTEGGGTCVLRANECPEKLHTVGQPVPGCEIRLIDAQGNEVNSGEIGEIVGRSNMMMT
EEECCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEE
GYHGRPDTTREATWIDANGNRYIRHGDLGRFDEEGFLTLLGRIKDMIISGGFNIYPSDIE
ECCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
AVLRLHPSIADCAVIGIPSVTWGETPFAFYVPKNDSLSPNEVITWVNQRMGKTQRLSDAQ
HHHHHCCCHHHEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHH
AIAQLPLSATGKVLKRELRALFLTEHPTPFAL
HHHHCCCHHHHHHHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure
METIHLGKSNFSSISELISIHAIERADTVALRYRDTVVTYKELQRYSEQILAAIQYNDIP
CCEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCC
PRTAIAIASSNPLLSTLALLGVISAGCVAVPVALSSSPEQIASVIADACIPLVFLDSEFL
CCEEEEEECCCHHHHHHHHHHHHHCCHHEEEEECCCCHHHHHHHHHHHHHHEEEECCHHH
EGLPSIGARVANLDEFDSWLAPEGFAATHVELSPADPFNIIYSSGTTGTPKGIIHTHGMR
CCCHHHHHHHCCCHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCEEEECCCC
WSQIAAYGQIVPQCDSAVTLIATPIYSNTTIVSLLPSLAYGGTAVLMGKFDAYEYLVEAQ
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCC
NSRATHTLMVPVQYQRIMALTKFDDFDLSSFIFKSCTGAPFSPELKADVIRRWPGALLEI
CCCCEEEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEE
YGMTEGGGTCVLRANECPEKLHTVGQPVPGCEIRLIDAQGNEVNSGEIGEIVGRSNMMMT
EEECCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEE
GYHGRPDTTREATWIDANGNRYIRHGDLGRFDEEGFLTLLGRIKDMIISGGFNIYPSDIE
ECCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
AVLRLHPSIADCAVIGIPSVTWGETPFAFYVPKNDSLSPNEVITWVNQRMGKTQRLSDAQ
HHHHHCCCHHHEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHH
AIAQLPLSATGKVLKRELRALFLTEHPTPFAL
HHHHCCCHHHHHHHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]