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The map label for this gene is rph [H]

Identifier: 157373079

GI number: 157373079

Start: 5365179

End: 5365895

Strand: Reverse

Name: rph [H]

Synonym: Spro_4847

Alternate gene names: 157373079

Gene position: 5365895-5365179 (Counterclockwise)

Preceding gene: 157373081

Following gene: 157373078

Centisome position: 98.48

GC content: 58.58

Gene sequence:

>717_bases
ATGCGTCCTGCAGGCAGAGCACCACAACAAGTTCGCCCACTGACACTGACCCGTCACTACACCAAACATGCAGAAGGCTC
AGTGCTGGTTGAGTTTGGCGATACCAAGGTTTTGTGCACCGCCACGGTAGAAGAGGGCGTTCCGCGCTTCCTGAAAGGTC
AGGGCCAGGGTTGGATCACCGCTGAATACGGCATGCTGCCGCGTTCTACCCACAGCCGTAACGCCCGCGAAGCCGCAAAA
GGCAAGCAGGGTGGCCGTACGCTGGAAATTCAGCGTCTGATCGCCCGTTCACTACGTGCAGCGGTAGATCTGAAAAAGCT
TGGTGAGTTCACCATCACGCTCGACTGCGACGTCTTGCAGGCCGATGGTGGCACCCGTACCGCGTCTATTTCCGGTGCCT
GTGTTGCGTTGGCCGATGCGTTGAATGCGCTGGTGGCTAACGGCAAGCTGAAAGCCAACCCGATGAAAGGGCTGGTCGCT
GCAGTTTCCGTCGGCATTGTTAACGGCGAAGCGCTGTGTGATTTGGAGTACGTCGAAGACTCTGCAGCAGAAACCGATAT
GAATGTGGTGATGATGGAAGATGGCCGCATGATCGAGGTGCAGGGCACCGCCGAGGGCGAGCCGTTCAGCCACGATGAGC
TATTGGCGCTGTTGGCGCTGGCCCGAGGGGGCATCGATACCATCTTCCAGGCGCAGAAAGCGGCGCTGGCAGACTAA

Upstream 100 bases:

>100_bases
TGAAGCGATTATAGCGTTGGAGATGCGGGCAGGATAGGCATTACGTCACTAAGCCCGTATAATGCGCAGCCAATCGTTGA
TTAGAAGCCGGAGAGAGCCC

Downstream 100 bases:

>100_bases
TTATTAAGGCGACTCAAGGGTCGCCTTTTTTACGCCCGGCGATCGGGCGGCAGTATTAACAGATCTAACGACCAGACGAG
GAGAAGCAGTAATGAAAGCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPAGRAPQQVRPLTLTRHYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWITAEYGMLPRSTHSRNAREAAK
GKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQADGGTRTASISGACVALADALNALVANGKLKANPMKGLVA
AVSVGIVNGEALCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHDELLALLALARGGIDTIFQAQKAALAD

Sequences:

>Translated_238_residues
MRPAGRAPQQVRPLTLTRHYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWITAEYGMLPRSTHSRNAREAAK
GKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQADGGTRTASISGACVALADALNALVANGKLKANPMKGLVA
AVSVGIVNGEALCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHDELLALLALARGGIDTIFQAQKAALAD
>Mature_238_residues
MRPAGRAPQQVRPLTLTRHYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWITAEYGMLPRSTHSRNAREAAK
GKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQADGGTRTASISGACVALADALNALVANGKLKANPMKGLVA
AVSVGIVNGEALCDLEYVEDSAAETDMNVVMMEDGRMIEVQGTAEGEPFSHDELLALLALARGGIDTIFQAQKAALAD

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=223, Percent_Identity=89.237668161435, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=27.807486631016, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115534063, Length=256, Percent_Identity=26.5625, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25260; Mature: 25260

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAGRAPQQVRPLTLTRHYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWIT
CCCCCCCCCCCCCEEEEHHHHCCCCCCEEEEECCCEEEEEEEHHHCCHHHHCCCCCEEEE
AEYGMLPRSTHSRNAREAAKGKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGACVALADALNALVANGKLKANPMKGLVAAVSVGIVNGEALCDLEYVED
CCCCCEEEECCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHEEEECCCEEEEHHHHCC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHDELLALLALARGGIDTIFQAQKAALAD
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPAGRAPQQVRPLTLTRHYTKHAEGSVLVEFGDTKVLCTATVEEGVPRFLKGQGQGWIT
CCCCCCCCCCCCCEEEEHHHHCCCCCCEEEEECCCEEEEEEEHHHCCHHHHCCCCCEEEE
AEYGMLPRSTHSRNAREAAKGKQGGRTLEIQRLIARSLRAAVDLKKLGEFTITLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGACVALADALNALVANGKLKANPMKGLVAAVSVGIVNGEALCDLEYVED
CCCCCEEEECCCHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHEEEECCCEEEEHHHHCC
SAAETDMNVVMMEDGRMIEVQGTAEGEPFSHDELLALLALARGGIDTIFQAQKAALAD
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA