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The map label for this gene is kbl [C]

Identifier: 157373056

GI number: 157373056

Start: 5343650

End: 5344846

Strand: Reverse

Name: kbl [C]

Synonym: Spro_4824

Alternate gene names: 157373056

Gene position: 5344846-5343650 (Counterclockwise)

Preceding gene: 157373062

Following gene: 157373055

Centisome position: 98.09

GC content: 57.31

Gene sequence:

>1197_bases
ATGTCCGCGTCTTTTTATCAGCAGTTGGAACAACAACTTGAGACCACCCGCAGCGAAGGTTTGTTCAAAGAAGAACGCAT
TATTACCAGCGCTCAGCAGGCGGATATTGCCGTTGCTGACGGCCGCCATGTGATCAACTTCTGCGCCAACAACTATCTTG
GCCTGGCCAACCACCCGGCAATGATTGCGGCAGCCAAGGCCGGTATGGACAGCCACGGTTTCGGCATGGCGTCGGTACGC
TTTATCTGCGGCACCCAGGACAGCCATAAACAGCTGGAGCAAAAGCTGGCCGATTTTCTCGGTATGGAAGACGCCATTTT
GTACTCCTCCTGCTTTGATGCCAACGGCGGGCTGTTCGAAACCCTGTTGGGCCCGGAAGACGCGATTATCTCCGATGCGT
TAAACCATGCGTCGATCATTGACGGCGTTCGCCTGTGCAAGGCGAAGCGCTACCGTTACGCCAACAACGACATGACCGAA
CTGGCTGCGCAGCTTGAGCAGGCGAAAGCCGACGGCGCACGCCACATTATGATCGCCACCGACGGCGTGTTCTCAATGGA
CGGTGTGATCGCCAACCTGAAAGGGGTGTGTGACCTGGCAGACCAATATCAGGCGCTGGTGATGGTCGATGACTCCCACG
CGGTCGGTTTTGTCGGGGCCAACGGGCGCGGCACCCACGAATATTGTGAAGTCATGGGTCGCGTCGACATCATTACCGGC
ACCCTGGGCAAGGCATTGGGCGGCGCTTCCGGCGGATATACCGCAGCGAAAAAGGAAGTGGTGGAGTGGCTGCGTCAGCG
TTCGCGCCCATATCTGTTCTCCAACTCGTTGGCACCGGCGATTGTCGCCGCTTCGATTAAAGTCCTGGAACTGCTGGAGC
AGGGTGACGCGTTGCGCGACCGCCTGTGGGCCAATGCCAGCCTGTTCCGTGAAAAAATGACCGCGGCGGGCTTTACGCTG
GCCGGGGCCGATCACGCCATCATCCCGGTGATGTTGGGTGAAGCAAAACTGGCGCAGGAATTTGCCAATGCGTTGCTCAA
GGAAGGCATTTATGTAACGGGTTTCTTCTATCCGGTGGTGCCGAAAGGCCAGGCGCGCATCCGTACCCAGATGTCCGCCG
ATCACACCCCGGAGCAAATTGAACGTGCGATTGCGGCGTTCATTCGTATCGGTAAAGATCTTGGCGTTATTGCATAA

Upstream 100 bases:

>100_bases
TATCTGCTGCAATTTTCATATTCTCTCCCGCAGATTCTCGTCAGTTTGGTTACAAATCAGTAAAATATGTTGAAAACAGA
CCAATCCTGGAGATAACCGA

Downstream 100 bases:

>100_bases
GGTAAAACCATGAAAGCATTGTCAAAACTGAAAGCGGAAGAAGGGATTTGGATGACCGATGTGCCTCAGCCGGAGCTGGG
CCATAACGACATCATGATTA

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSASFYQQLEQQLETTRSEGLFKEERIITSAQQADIAVADGRHVINFCANNYLGLANHPAMIAAAKAGMDSHGFGMASVR
FICGTQDSHKQLEQKLADFLGMEDAILYSSCFDANGGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANNDMTE
LAAQLEQAKADGARHIMIATDGVFSMDGVIANLKGVCDLADQYQALVMVDDSHAVGFVGANGRGTHEYCEVMGRVDIITG
TLGKALGGASGGYTAAKKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLELLEQGDALRDRLWANASLFREKMTAAGFTL
AGADHAIIPVMLGEAKLAQEFANALLKEGIYVTGFFYPVVPKGQARIRTQMSADHTPEQIERAIAAFIRIGKDLGVIA

Sequences:

>Translated_398_residues
MSASFYQQLEQQLETTRSEGLFKEERIITSAQQADIAVADGRHVINFCANNYLGLANHPAMIAAAKAGMDSHGFGMASVR
FICGTQDSHKQLEQKLADFLGMEDAILYSSCFDANGGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANNDMTE
LAAQLEQAKADGARHIMIATDGVFSMDGVIANLKGVCDLADQYQALVMVDDSHAVGFVGANGRGTHEYCEVMGRVDIITG
TLGKALGGASGGYTAAKKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLELLEQGDALRDRLWANASLFREKMTAAGFTL
AGADHAIIPVMLGEAKLAQEFANALLKEGIYVTGFFYPVVPKGQARIRTQMSADHTPEQIERAIAAFIRIGKDLGVIA
>Mature_397_residues
SASFYQQLEQQLETTRSEGLFKEERIITSAQQADIAVADGRHVINFCANNYLGLANHPAMIAAAKAGMDSHGFGMASVRF
ICGTQDSHKQLEQKLADFLGMEDAILYSSCFDANGGLFETLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANNDMTEL
AAQLEQAKADGARHIMIATDGVFSMDGVIANLKGVCDLADQYQALVMVDDSHAVGFVGANGRGTHEYCEVMGRVDIITGT
LGKALGGASGGYTAAKKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLELLEQGDALRDRLWANASLFREKMTAAGFTLA
GADHAIIPVMLGEAKLAQEFANALLKEGIYVTGFFYPVVPKGQARIRTQMSADHTPEQIERAIAAFIRIGKDLGVIA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=391, Percent_Identity=55.7544757033248, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI284448556, Length=417, Percent_Identity=52.2781774580336, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI83977442, Length=373, Percent_Identity=35.9249329758713, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI83977440, Length=373, Percent_Identity=35.9249329758713, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI83977444, Length=373, Percent_Identity=35.9249329758713, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI4502025, Length=360, Percent_Identity=34.1666666666667, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI40316939, Length=360, Percent_Identity=34.1666666666667, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI119220554, Length=366, Percent_Identity=32.5136612021858, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4758668, Length=364, Percent_Identity=32.967032967033, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI5454084, Length=389, Percent_Identity=24.9357326478149, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1790046, Length=398, Percent_Identity=86.6834170854271, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI1786993, Length=350, Percent_Identity=35.1428571428571, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71994529, Length=396, Percent_Identity=54.7979797979798, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32566772, Length=368, Percent_Identity=30.1630434782609, Blast_Score=180, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17560914, Length=366, Percent_Identity=31.9672131147541, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17560912, Length=366, Percent_Identity=31.9672131147541, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71982625, Length=378, Percent_Identity=24.6031746031746, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71982617, Length=378, Percent_Identity=24.6031746031746, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320438, Length=359, Percent_Identity=32.3119777158774, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6320267, Length=371, Percent_Identity=30.9973045822102, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323954, Length=266, Percent_Identity=29.6992481203008, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24662918, Length=396, Percent_Identity=58.0808080808081, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17137420, Length=349, Percent_Identity=32.9512893982808, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17136286, Length=369, Percent_Identity=32.2493224932249, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24653280, Length=348, Percent_Identity=25.5747126436782, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653276, Length=348, Percent_Identity=25.5747126436782, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653278, Length=348, Percent_Identity=25.5747126436782, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 42959; Mature: 42827

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASFYQQLEQQLETTRSEGLFKEERIITSAQQADIAVADGRHVINFCANNYLGLANHPA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCCCCCCCH
MIAAAKAGMDSHGFGMASVRFICGTQDSHKQLEQKLADFLGMEDAILYSSCFDANGGLFE
HHHHHHCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHH
TLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANNDMTELAAQLEQAKADGARHIMIAT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEE
DGVFSMDGVIANLKGVCDLADQYQALVMVDDSHAVGFVGANGRGTHEYCEVMGRVDIITG
CCCEEHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCCHHHHHHHHCCHHEEHH
TLGKALGGASGGYTAAKKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLELLEQGDALRD
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCHHHH
RLWANASLFREKMTAAGFTLAGADHAIIPVMLGEAKLAQEFANALLKEGIYVTGFFYPVV
HHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEECC
PKGQARIRTQMSADHTPEQIERAIAAFIRIGKDLGVIA
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SASFYQQLEQQLETTRSEGLFKEERIITSAQQADIAVADGRHVINFCANNYLGLANHPA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCCCCCCCH
MIAAAKAGMDSHGFGMASVRFICGTQDSHKQLEQKLADFLGMEDAILYSSCFDANGGLFE
HHHHHHCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHH
TLLGPEDAIISDALNHASIIDGVRLCKAKRYRYANNDMTELAAQLEQAKADGARHIMIAT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEE
DGVFSMDGVIANLKGVCDLADQYQALVMVDDSHAVGFVGANGRGTHEYCEVMGRVDIITG
CCCEEHHHHHHHHHHHHHHHHCCEEEEEEECCCEEEEEECCCCCHHHHHHHHCCHHEEHH
TLGKALGGASGGYTAAKKEVVEWLRQRSRPYLFSNSLAPAIVAASIKVLELLEQGDALRD
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHCCHHHH
RLWANASLFREKMTAAGFTLAGADHAIIPVMLGEAKLAQEFANALLKEGIYVTGFFYPVV
HHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEECC
PKGQARIRTQMSADHTPEQIERAIAAFIRIGKDLGVIA
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]