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The map label for this gene is metF [H]

Identifier: 157373016

GI number: 157373016

Start: 5301631

End: 5302524

Strand: Reverse

Name: metF [H]

Synonym: Spro_4784

Alternate gene names: 157373016

Gene position: 5302524-5301631 (Counterclockwise)

Preceding gene: 157373017

Following gene: 157373013

Centisome position: 97.31

GC content: 56.04

Gene sequence:

>894_bases
ATGAGTTTTTTTCACGCAAACCAGCGGGAAGCGCTGAATCAGAGTCTGGCAGAGTTAAACGGCCAGATTAACGTATCATT
CGAGTTCTTCCCGCCGCGCACCAGCGAGATGGAAGACACCCTGTGGCAGTCCATCGATCGTCTGAGCAGCCTGAAACCTA
AATTTGTCTCTGTGACCTATGGTGCCAATTCCGGCGAGCGCGATCGTACCCACAGCATTATCAAAGGGATTAAGGAGCGT
ACCGGTCTGGAGGCAGCACCGCACCTGACCTGCATCGACGCCAGCCCGGCTCAGCTGCGTGAGATTGCTACCGACTACTG
GAACAGCGGCATCCGTCATATCGTGGCGCTGCGTGGCGACCTGCCACCGGGCAGCGGTAAGCCCGATATGTACGCTACCG
ATTTGGTGGCCTTGCTCAAAGACGTCGGCGATTTTGATATCTCGGTGGCGGCCTACCCTGAAGTGCACCCGGAAGCCAAA
AGTCCGCAGGCGGATCTGATTAACCTGAAGCGTAAAATCGATGCCGGTGCCAACCGCGCGATTACCCAGTTCTTCTTTGA
TGTGGAAAGCTACCTGCGTTTTCGTGACCGCTGCGTGGCGACCGGTATTGACGTGGAAATCGTGCCGGGCATTCTGCCGG
TGTCCAACTTCAAGCAGTTGCAGCGTTTTGCCACCATGACTAACGTGCGTGTGCCTAGCTGGATGACCAGCATGTTTGAA
GGGCTGGACGACGATGCCGAAACCCGCAAAATGGTCGGTGCCAATATCGCGATGGATATGGTGAAAATTCTCAGCCGCGA
AGGGGTGAAAGACTTCCACTTCTATACGCTGAACCGCGCCGAGATGAGTTACGCCATTTGCCATACGTTGGGCGTGCGCC
CGGCGGCGGCATAA

Upstream 100 bases:

>100_bases
ACTCTTTAGTCAACATACGGCATTTTCTATCTAGACGTCTAAACGTATAGACGCTTAATAAGATAAAGCAAAACAGCGAT
CGATAAGAGGCGGACAGGGT

Downstream 100 bases:

>100_bases
TCTACTCTATTTGTCATTGAGAAGGGCGCTGGGTAAAGACGGCGCCCTTTTTGTTGGATAAAAAAACGGCCCCTGTGAAC
AGGAGCCGTTTTTTACAACG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSFFHANQREALNQSLAELNGQINVSFEFFPPRTSEMEDTLWQSIDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKER
TGLEAAPHLTCIDASPAQLREIATDYWNSGIRHIVALRGDLPPGSGKPDMYATDLVALLKDVGDFDISVAAYPEVHPEAK
SPQADLINLKRKIDAGANRAITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPSWMTSMFE
GLDDDAETRKMVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRPAAA

Sequences:

>Translated_297_residues
MSFFHANQREALNQSLAELNGQINVSFEFFPPRTSEMEDTLWQSIDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKER
TGLEAAPHLTCIDASPAQLREIATDYWNSGIRHIVALRGDLPPGSGKPDMYATDLVALLKDVGDFDISVAAYPEVHPEAK
SPQADLINLKRKIDAGANRAITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPSWMTSMFE
GLDDDAETRKMVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRPAAA
>Mature_296_residues
SFFHANQREALNQSLAELNGQINVSFEFFPPRTSEMEDTLWQSIDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKERT
GLEAAPHLTCIDASPAQLREIATDYWNSGIRHIVALRGDLPPGSGKPDMYATDLVALLKDVGDFDISVAAYPEVHPEAKS
PQADLINLKRKIDAGANRAITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPSWMTSMFEG
LDDDAETRKMVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRPAAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=303, Percent_Identity=34.6534653465347, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1790377, Length=295, Percent_Identity=88.135593220339, Blast_Score=559, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=32.3741007194245, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=32.3741007194245, Blast_Score=148, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6325234, Length=278, Percent_Identity=34.1726618705036, Blast_Score=153, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321313, Length=294, Percent_Identity=30.952380952381, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33150; Mature: 33019

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFHANQREALNQSLAELNGQINVSFEFFPPRTSEMEDTLWQSIDRLSSLKPKFVSVTY
CCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSIIKGIKERTGLEAAPHLTCIDASPAQLREIATDYWNSGIRHIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEHHEECC
LPPGSGKPDMYATDLVALLKDVGDFDISVAAYPEVHPEAKSPQADLINLKRKIDAGANRA
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPSWMTSMFE
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
GLDDDAETRKMVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRPAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SFFHANQREALNQSLAELNGQINVSFEFFPPRTSEMEDTLWQSIDRLSSLKPKFVSVTY
CCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSIIKGIKERTGLEAAPHLTCIDASPAQLREIATDYWNSGIRHIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHEEHHEECC
LPPGSGKPDMYATDLVALLKDVGDFDISVAAYPEVHPEAKSPQADLINLKRKIDAGANRA
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCVATGIDVEIVPGILPVSNFKQLQRFATMTNVRVPSWMTSMFE
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
GLDDDAETRKMVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRPAAA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA