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The map label for this gene is trmA

Identifier: 157373003

GI number: 157373003

Start: 5285613

End: 5286716

Strand: Direct

Name: trmA

Synonym: Spro_4771

Alternate gene names: 157373003

Gene position: 5285613-5286716 (Clockwise)

Preceding gene: 157372996

Following gene: 157373006

Centisome position: 97.0

GC content: 54.26

Gene sequence:

>1104_bases
ATGACGCCCGAGAATTTGCCTATTGAACGTTACGATGACCAACTGGCGGAGAAAACTGCCCGGCTGAAAACGCTGATGTC
TCCGTTCACGGCACCCGAGCCGGAAGTGTTCCGCTCGCCGGTAAGCCACTACCGCATGCGCGCCGAATTCCGCATCTGGC
ATGATGAAGACGAGATGTACCACATCATGTTTGATCAGCAGACCAAACAACGCATCCGCGTCGACAAGTTTCCTGCCGCC
AGTGAATTGATTAACCGCCTGATGAGCGCCCTGATCGCTGCCATCAAGCCGGAGCCGATTCTGCGCCGTAAGCTTTTCCA
GATTGACTACCTGTCGACCCGCAGCGGCAAAATCATTGCCTCACTGCTGTATCACCGCAAGCTGGACGACGAATGGCAGC
AGCAGGCAGAAAAGCTGCGTGATGACCTGCGCGCGCAGGGCTTTGACCTGCAGCTGATTGGCCGCGCGTCTAAAATGAAA
ATCATGCTGGATCAGGATTATGTAGACGAAGTGCTGCCGGTTGCCGGCCGCGACATGATCTATCGCCAGGTGGAAAACAG
CTTCACCCAGCCGAATGCGGCGCTGAATATACAGATGCTGGAATGGGCGCTGGACGTCACCACCGGTTCTAAAGGCGATT
TGCTGGAGTTGTATTGCGGTAACGGTAACTTCTCTTTGGCATTGGCGCGTAACTTCGAGCGGGTTCTGGCTACCGAAATC
GCCAAACCTTCGGTCGCTGCGGCACAGTACAATATTGCGGCCAACCAGATCGATAACGTGCAGATTATCCGTATGGCAGC
GGAAGACTTTACCCAGGCGATGAACGGAGTGCGTGAGTTTAATCGGTTGAAGGGTATCGATTTAAGCAGCTATAACTGCG
AAACCATTTTCGTCGATCCGCCACGCAGTGGGCTGGACGATGAAACGGTGAAAATGGTGCAGGCCTATCCGCGTATTTTG
TATATCTCATGCAACCCGGAAACGCTGTGTGCCAATCTGGAGACCTTGCAGCAAACCCACAGCATCAGCCGCCTGGCGCT
GTTCGATCAGTTCCCGTACACCCATCACATGGAATGCGGCGTGCTGTTGGAAAAACGCGTCTAA

Upstream 100 bases:

>100_bases
ACTGCTATCAATGTTACAAATAATAAACACGACAAAACTGGACTTCACGGACTGATTCCCTACAATCCCCGCTGATCAAT
TTTGCCTGACGAGAAGAAAC

Downstream 100 bases:

>100_bases
TCTGACAGGGGCGCCATTATTGCTGCGCCCCTGCTTTACGCCCATCAGGCCGGCGTATTCTCATCCAGCGCCTTGCGCGC
CTTCAGCTTGATACCAATCC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTPENLPIERYDDQLAEKTARLKTLMSPFTAPEPEVFRSPVSHYRMRAEFRIWHDEDEMYHIMFDQQTKQRIRVDKFPAA
SELINRLMSALIAAIKPEPILRRKLFQIDYLSTRSGKIIASLLYHRKLDDEWQQQAEKLRDDLRAQGFDLQLIGRASKMK
IMLDQDYVDEVLPVAGRDMIYRQVENSFTQPNAALNIQMLEWALDVTTGSKGDLLELYCGNGNFSLALARNFERVLATEI
AKPSVAAAQYNIAANQIDNVQIIRMAAEDFTQAMNGVREFNRLKGIDLSSYNCETIFVDPPRSGLDDETVKMVQAYPRIL
YISCNPETLCANLETLQQTHSISRLALFDQFPYTHHMECGVLLEKRV

Sequences:

>Translated_367_residues
MTPENLPIERYDDQLAEKTARLKTLMSPFTAPEPEVFRSPVSHYRMRAEFRIWHDEDEMYHIMFDQQTKQRIRVDKFPAA
SELINRLMSALIAAIKPEPILRRKLFQIDYLSTRSGKIIASLLYHRKLDDEWQQQAEKLRDDLRAQGFDLQLIGRASKMK
IMLDQDYVDEVLPVAGRDMIYRQVENSFTQPNAALNIQMLEWALDVTTGSKGDLLELYCGNGNFSLALARNFERVLATEI
AKPSVAAAQYNIAANQIDNVQIIRMAAEDFTQAMNGVREFNRLKGIDLSSYNCETIFVDPPRSGLDDETVKMVQAYPRIL
YISCNPETLCANLETLQQTHSISRLALFDQFPYTHHMECGVLLEKRV
>Mature_366_residues
TPENLPIERYDDQLAEKTARLKTLMSPFTAPEPEVFRSPVSHYRMRAEFRIWHDEDEMYHIMFDQQTKQRIRVDKFPAAS
ELINRLMSALIAAIKPEPILRRKLFQIDYLSTRSGKIIASLLYHRKLDDEWQQQAEKLRDDLRAQGFDLQLIGRASKMKI
MLDQDYVDEVLPVAGRDMIYRQVENSFTQPNAALNIQMLEWALDVTTGSKGDLLELYCGNGNFSLALARNFERVLATEIA
KPSVAAAQYNIAANQIDNVQIIRMAAEDFTQAMNGVREFNRLKGIDLSSYNCETIFVDPPRSGLDDETVKMVQAYPRILY
ISCNPETLCANLETLQQTHSISRLALFDQFPYTHHMECGVLLEKRV

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=203, Percent_Identity=28.0788177339901, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI51173878, Length=203, Percent_Identity=28.0788177339901, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790403, Length=366, Percent_Identity=80.0546448087432, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789148, Length=188, Percent_Identity=34.0425531914894, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787083, Length=372, Percent_Identity=26.3440860215054, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666579, Length=197, Percent_Identity=29.9492385786802, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_SERP5 (A8GL74)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480992.1
- ProteinModelPortal:   A8GL74
- SMR:   A8GL74
- STRING:   A8GL74
- GeneID:   5604631
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4771
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- BioCyc:   SPRO399741:SPRO_4771-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42276; Mature: 42145

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 324-324 BINDING 190-190 BINDING 218-218 BINDING 223-223 BINDING 239-239 BINDING 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPENLPIERYDDQLAEKTARLKTLMSPFTAPEPEVFRSPVSHYRMRAEFRIWHDEDEMY
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHEEEEEEEEECCCCEE
HIMFDQQTKQRIRVDKFPAASELINRLMSALIAAIKPEPILRRKLFQIDYLSTRSGKIIA
EEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCHHHH
SLLYHRKLDDEWQQQAEKLRDDLRAQGFDLQLIGRASKMKIMLDQDYVDEVLPVAGRDMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECHHHHHHHHCCCCHHHH
YRQVENSFTQPNAALNIQMLEWALDVTTGSKGDLLELYCGNGNFSLALARNFERVLATEI
HHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHH
AKPSVAAAQYNIAANQIDNVQIIRMAAEDFTQAMNGVREFNRLKGIDLSSYNCETIFVDP
CCCCHHHHEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PRSGLDDETVKMVQAYPRILYISCNPETLCANLETLQQTHSISRLALFDQFPYTHHMECG
CCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLEKRV
CEEECCC
>Mature Secondary Structure 
TPENLPIERYDDQLAEKTARLKTLMSPFTAPEPEVFRSPVSHYRMRAEFRIWHDEDEMY
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHEEEEEEEEECCCCEE
HIMFDQQTKQRIRVDKFPAASELINRLMSALIAAIKPEPILRRKLFQIDYLSTRSGKIIA
EEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCHHHH
SLLYHRKLDDEWQQQAEKLRDDLRAQGFDLQLIGRASKMKIMLDQDYVDEVLPVAGRDMI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECHHHHHHHHCCCCHHHH
YRQVENSFTQPNAALNIQMLEWALDVTTGSKGDLLELYCGNGNFSLALARNFERVLATEI
HHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHH
AKPSVAAAQYNIAANQIDNVQIIRMAAEDFTQAMNGVREFNRLKGIDLSSYNCETIFVDP
CCCCHHHHEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PRSGLDDETVKMVQAYPRILYISCNPETLCANLETLQQTHSISRLALFDQFPYTHHMECG
CCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VLLEKRV
CEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA