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The map label for this gene is yiaO [H]

Identifier: 157372937

GI number: 157372937

Start: 5199855

End: 5200841

Strand: Reverse

Name: yiaO [H]

Synonym: Spro_4705

Alternate gene names: 157372937

Gene position: 5200841-5199855 (Counterclockwise)

Preceding gene: 157372938

Following gene: 157372936

Centisome position: 95.45

GC content: 47.72

Gene sequence:

>987_bases
ATGAAAGGACAACTTAAGCGCTGGGTCATATTGACCTGCGCATTATTTTCTTTACTTGTTCTCAGCACGGAATCAATTTC
AGCAAAATATACTATACGGGTTTCTTATGAAAATAACCCGGGTGAGCCTTTTGATCTGGCGGTAAGAAAGTGGGCTGAAC
TCTTTAAAGAAAAAACTAACGGCCAAGGCGAACTTATTTTGTTCCCCAGCTCTTCATTAGGATCAAAAAAAGACGTGATG
GAGCAAATCAAGCTGGGGAGTGCGATGATCACCCTGGCAGATGGGGCCTTTTTTGCCGACTATATACCCGATTTCGGCAT
TATGATGGGGCCGTATCTGGGGCGCGATTACAACGATATTTTCACCTTGACCAAAACGCCCTGGTTTACCGACCTGTCAC
GCCAACTGGACGACAAAGGTTTTCATATCCTGGCGGCTAACTGGCTGTACGGTATCCGCCAGATGGTGGTGAACAAACCC
ATCCATACCCCGGCGGACCTGAAAGGCGTAAAAATCCGCGTCGCCAACAGCCGTATCTTTATTGAAGCCATCAAGGCGAT
GGGGGCAACCCCAACCCCCATGCCATTGGGCGAAGTGTATACCGCGTTGACTACCGGAGTGATCGACGGTGCGGAAAACC
CAATCCCGGTACTGTACGGCCAAAAGCTTTTTGAGCCGGCCAGGTACCTGATCATGACCGGGCATATTGATAATGTCACC
CAACTGATCATTGGTCAGAAATATTACAGCCAACTTCCTGATGAGATTAAAAAAGCGTTAACGCTATCCTGTGAGGAAGC
CGGTAATTATATGACCAGGTTAATTATCCAGGCTGATAAGCAAGATCGGGAAAAAATGAAAGCGGCGGGCGTGACGGTGA
TTGAGGTTGACCGTGAATTATTCCGGCAGGCGTCAAAGTCGGCCTATCAGAAATTCCCTGAATGGACGCCGGGATTATAT
GAAAAGCTTCAGGGCTATTTAGAATAA

Upstream 100 bases:

>100_bases
CAATTTGAACAACTCAATCAGCCAGGCAATGTGAAGTCGGTCATTCTATTTTAAGCATCCGCAGTCGGTTACCTGAATAA
TTGAACTGGAGATTATTATT

Downstream 100 bases:

>100_bases
TTGACTGCCATTTTTCGACAACTTCCGCTGCATCGCACCGGGGGAGTAATAATCATGATCCGAAAGTTATTGGTTAATCT
TGACGATATTCTCGCCTGCC

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKGQLKRWVILTCALFSLLVLSTESISAKYTIRVSYENNPGEPFDLAVRKWAELFKEKTNGQGELILFPSSSLGSKKDVM
EQIKLGSAMITLADGAFFADYIPDFGIMMGPYLGRDYNDIFTLTKTPWFTDLSRQLDDKGFHILAANWLYGIRQMVVNKP
IHTPADLKGVKIRVANSRIFIEAIKAMGATPTPMPLGEVYTALTTGVIDGAENPIPVLYGQKLFEPARYLIMTGHIDNVT
QLIIGQKYYSQLPDEIKKALTLSCEEAGNYMTRLIIQADKQDREKMKAAGVTVIEVDRELFRQASKSAYQKFPEWTPGLY
EKLQGYLE

Sequences:

>Translated_328_residues
MKGQLKRWVILTCALFSLLVLSTESISAKYTIRVSYENNPGEPFDLAVRKWAELFKEKTNGQGELILFPSSSLGSKKDVM
EQIKLGSAMITLADGAFFADYIPDFGIMMGPYLGRDYNDIFTLTKTPWFTDLSRQLDDKGFHILAANWLYGIRQMVVNKP
IHTPADLKGVKIRVANSRIFIEAIKAMGATPTPMPLGEVYTALTTGVIDGAENPIPVLYGQKLFEPARYLIMTGHIDNVT
QLIIGQKYYSQLPDEIKKALTLSCEEAGNYMTRLIIQADKQDREKMKAAGVTVIEVDRELFRQASKSAYQKFPEWTPGLY
EKLQGYLE
>Mature_328_residues
MKGQLKRWVILTCALFSLLVLSTESISAKYTIRVSYENNPGEPFDLAVRKWAELFKEKTNGQGELILFPSSSLGSKKDVM
EQIKLGSAMITLADGAFFADYIPDFGIMMGPYLGRDYNDIFTLTKTPWFTDLSRQLDDKGFHILAANWLYGIRQMVVNKP
IHTPADLKGVKIRVANSRIFIEAIKAMGATPTPMPLGEVYTALTTGVIDGAENPIPVLYGQKLFEPARYLIMTGHIDNVT
QLIIGQKYYSQLPDEIKKALTLSCEEAGNYMTRLIIQADKQDREKMKAAGVTVIEVDRELFRQASKSAYQKFPEWTPGLY
EKLQGYLE

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=298, Percent_Identity=35.5704697986577, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36916; Mature: 36916

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGQLKRWVILTCALFSLLVLSTESISAKYTIRVSYENNPGEPFDLAVRKWAELFKEKTN
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
GQGELILFPSSSLGSKKDVMEQIKLGSAMITLADGAFFADYIPDFGIMMGPYLGRDYNDI
CCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHCCCCHHHHCCCCCCCCCCE
FTLTKTPWFTDLSRQLDDKGFHILAANWLYGIRQMVVNKPIHTPADLKGVKIRVANSRIF
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHH
IEAIKAMGATPTPMPLGEVYTALTTGVIDGAENPIPVLYGQKLFEPARYLIMTGHIDNVT
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHCCCEEEEEECCHHHHH
QLIIGQKYYSQLPDEIKKALTLSCEEAGNYMTRLIIQADKQDREKMKAAGVTVIEVDREL
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHEECCHHHHHHHHHCCCEEEEHHHHH
FRQASKSAYQKFPEWTPGLYEKLQGYLE
HHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKGQLKRWVILTCALFSLLVLSTESISAKYTIRVSYENNPGEPFDLAVRKWAELFKEKTN
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
GQGELILFPSSSLGSKKDVMEQIKLGSAMITLADGAFFADYIPDFGIMMGPYLGRDYNDI
CCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHCCCCHHHHCCCCCCCCCCE
FTLTKTPWFTDLSRQLDDKGFHILAANWLYGIRQMVVNKPIHTPADLKGVKIRVANSRIF
EEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHH
IEAIKAMGATPTPMPLGEVYTALTTGVIDGAENPIPVLYGQKLFEPARYLIMTGHIDNVT
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCHHHHCCCEEEEEECCHHHHH
QLIIGQKYYSQLPDEIKKALTLSCEEAGNYMTRLIIQADKQDREKMKAAGVTVIEVDREL
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHEECCHHHHHHHHHCCCEEEEHHHHH
FRQASKSAYQKFPEWTPGLYEKLQGYLE
HHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]