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The map label for this gene is yfkN [H]

Identifier: 157372931

GI number: 157372931

Start: 5192384

End: 5194234

Strand: Reverse

Name: yfkN [H]

Synonym: Spro_4699

Alternate gene names: 157372931

Gene position: 5194234-5192384 (Counterclockwise)

Preceding gene: 157372932

Following gene: 157372922

Centisome position: 95.33

GC content: 55.97

Gene sequence:

>1851_bases
ATGGCCTCTGAACCTGTCAATATCACCATTCTTGGCACTTCTGATCTGCACGGCACCTTTGTGCCCTGGGATTATTCTAC
CGATACCGCCAATCTGGCCGGCAGCCTGAGCCAAATTGCCACTCAGGTGCATCAGGTGCGCGCCCAGCAGCCGAACGTGA
TCCTGGTCGATGCCGGTGACACCATTCAGGGCAACTTTGTCGAGACCTTTAAAAACGACAAAACCAGCCCGATGATCCTC
GGCTTTAACGCGCTGGACTATGACGTCTGGGTGATGGGTAATCACGAATTCGACTTCGGCCTCAAACCGCTTTCCACTTC
ACTCAATCAATTCAAAGGCACGGCATTGGCCGGCAACATTCTCTGGGACAGCGGTAAGCCCTACCTGCCGGCGTATAAAA
TCATTGAACGCCAGGGAGTGAAAATTGGCATTATCGGCATGGATACGCCGATGACTGCCGAATTCGCCAAAGGCACCGAC
CGGGTGAAAGGACTGAACTTTACCGATCCGGTGCAGGCGGTGAAGCAGGTGATCCAGCAGATCCAGGGGCAAGTGGACGC
CATCGTGCTGGTGGCACACATGGGGATCGACAACGAGAACCAGCGGCCGGGGACCGGCGTGGGCGATATTGCCAGGGCCA
ACCCGGAACTGGCGGCGATTGTCGCCGGGCATATGCACGTAAAAGTCGATAAAGAAGTGATTAACGGCGTGATCGTCACC
GAACCGGACAAATATGGCCGCGCGCTGTCACGCATCGATCTGCAGTTTGAGCAGCACAACGGCAAGTACGTGCTGATCAA
CAAAGACAGTTACACCTACCCGATCAAAGGCATCGCCTCGGATAAAAAACTCGAAGAGATTTATCAGCCGTACCACAAGA
TTTTGCGCGCTAACGCCAACCGACCGATTGCCCAGCTTAGCGGCCAGGATCTGGTACCGCCGGATGCGGTGAAGGGCATT
CCGCAGGTGCACGTGCAGGATACCGGTATCAGCAAGCTGTATCAGGAAGCCGGGCATTACTATGCACCGCAGGCGCAGGT
GATCGCGTTGCAGATAGATAACGACCGCCCGAAGCTGAACGTCGGTACCATCACCGCCAAAGACATTGCCTTCAACTACC
AATATGCCGGTGGCGAGATCACCGTTTATCAGCTCAGCGGCAAAGAATTGAAGAAGTATATGGAATGGTCGGCGGGCTAC
TTCAATCAGCTGCATGACGGCGACGTGACCTACAGTTTTAACCCGCAGCGTCGCGCGTCCAAATATTCCACCAATGATTT
CTTCGATGGCGTGACCTACAAAATCGACCTGCGCCAACCGGCCGGGCAGCGCATCCGCGACCTGAAAATGGCTGACGGCA
CGCCGGTGACGGACAGCACGCCGATCCGTCTGGGAATGAACAGCTACCGCATGGGGCACCTGACGCAAAAAGGCGGTGCG
CTGGAAGGCATGCAGTTCCCGGTGCTGTCTGACAGCAAGGTGGAATACGGTGAAGAGGCCGGTACCATCCGCAATCTGAC
CATTCGCTATCTGACCGAGGTGAAAAAGGGCCAGTATCAGGGGCAGGCGGTGCAGCGCTGGCAGTTGGTCGGGCTGGAAG
GCTACGAACGTGAGCGTAAAATCGTCGAAAAACTGCTGAACGACGGCAAAATCAGCGTACCTACCAGTGAAGATGGGCGT
TACGGCAACGTGGCGTCATTCAACGTGAAAGCGCTGTTGATCGGCGAACCGCAGGCCCGTCAGCAGCAACAGGCCGCGCT
GAAGGCACAGTTGAGCGCCGCCAGCGATCCGCTGGTGAAAAAGCGTTTGAGTGACCAGTTGGTGATTATCGAGGCGATTA
ACAAAGCCTGA

Upstream 100 bases:

>100_bases
CCAACCAGATCGCATGATTCCTGTTCATTTCCACTCCTATAATTAGATGATAATGAAGAAGACACTCCTTGCCGTTTTGC
TTTGCCTGGGTACCTCTGCG

Downstream 100 bases:

>100_bases
GGGTGATAACGGGCTCGGCATGTGAATGCTGAGCCCTATCGGCACTAATTCCCCTGCCGTAAACGCTTGCCGACGTCACC
GCCCGGGTGTACCGCCAGCA

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MASEPVNITILGTSDLHGTFVPWDYSTDTANLAGSLSQIATQVHQVRAQQPNVILVDAGDTIQGNFVETFKNDKTSPMIL
GFNALDYDVWVMGNHEFDFGLKPLSTSLNQFKGTALAGNILWDSGKPYLPAYKIIERQGVKIGIIGMDTPMTAEFAKGTD
RVKGLNFTDPVQAVKQVIQQIQGQVDAIVLVAHMGIDNENQRPGTGVGDIARANPELAAIVAGHMHVKVDKEVINGVIVT
EPDKYGRALSRIDLQFEQHNGKYVLINKDSYTYPIKGIASDKKLEEIYQPYHKILRANANRPIAQLSGQDLVPPDAVKGI
PQVHVQDTGISKLYQEAGHYYAPQAQVIALQIDNDRPKLNVGTITAKDIAFNYQYAGGEITVYQLSGKELKKYMEWSAGY
FNQLHDGDVTYSFNPQRRASKYSTNDFFDGVTYKIDLRQPAGQRIRDLKMADGTPVTDSTPIRLGMNSYRMGHLTQKGGA
LEGMQFPVLSDSKVEYGEEAGTIRNLTIRYLTEVKKGQYQGQAVQRWQLVGLEGYERERKIVEKLLNDGKISVPTSEDGR
YGNVASFNVKALLIGEPQARQQQQAALKAQLSAASDPLVKKRLSDQLVIIEAINKA

Sequences:

>Translated_616_residues
MASEPVNITILGTSDLHGTFVPWDYSTDTANLAGSLSQIATQVHQVRAQQPNVILVDAGDTIQGNFVETFKNDKTSPMIL
GFNALDYDVWVMGNHEFDFGLKPLSTSLNQFKGTALAGNILWDSGKPYLPAYKIIERQGVKIGIIGMDTPMTAEFAKGTD
RVKGLNFTDPVQAVKQVIQQIQGQVDAIVLVAHMGIDNENQRPGTGVGDIARANPELAAIVAGHMHVKVDKEVINGVIVT
EPDKYGRALSRIDLQFEQHNGKYVLINKDSYTYPIKGIASDKKLEEIYQPYHKILRANANRPIAQLSGQDLVPPDAVKGI
PQVHVQDTGISKLYQEAGHYYAPQAQVIALQIDNDRPKLNVGTITAKDIAFNYQYAGGEITVYQLSGKELKKYMEWSAGY
FNQLHDGDVTYSFNPQRRASKYSTNDFFDGVTYKIDLRQPAGQRIRDLKMADGTPVTDSTPIRLGMNSYRMGHLTQKGGA
LEGMQFPVLSDSKVEYGEEAGTIRNLTIRYLTEVKKGQYQGQAVQRWQLVGLEGYERERKIVEKLLNDGKISVPTSEDGR
YGNVASFNVKALLIGEPQARQQQQAALKAQLSAASDPLVKKRLSDQLVIIEAINKA
>Mature_615_residues
ASEPVNITILGTSDLHGTFVPWDYSTDTANLAGSLSQIATQVHQVRAQQPNVILVDAGDTIQGNFVETFKNDKTSPMILG
FNALDYDVWVMGNHEFDFGLKPLSTSLNQFKGTALAGNILWDSGKPYLPAYKIIERQGVKIGIIGMDTPMTAEFAKGTDR
VKGLNFTDPVQAVKQVIQQIQGQVDAIVLVAHMGIDNENQRPGTGVGDIARANPELAAIVAGHMHVKVDKEVINGVIVTE
PDKYGRALSRIDLQFEQHNGKYVLINKDSYTYPIKGIASDKKLEEIYQPYHKILRANANRPIAQLSGQDLVPPDAVKGIP
QVHVQDTGISKLYQEAGHYYAPQAQVIALQIDNDRPKLNVGTITAKDIAFNYQYAGGEITVYQLSGKELKKYMEWSAGYF
NQLHDGDVTYSFNPQRRASKYSTNDFFDGVTYKIDLRQPAGQRIRDLKMADGTPVTDSTPIRLGMNSYRMGHLTQKGGAL
EGMQFPVLSDSKVEYGEEAGTIRNLTIRYLTEVKKGQYQGQAVQRWQLVGLEGYERERKIVEKLLNDGKISVPTSEDGRY
GNVASFNVKALLIGEPQARQQQQAALKAQLSAASDPLVKKRLSDQLVIIEAINKA

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=206, Percent_Identity=29.6116504854369, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790658, Length=608, Percent_Identity=25.6578947368421, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1786687, Length=285, Percent_Identity=29.1228070175439, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922446, Length=456, Percent_Identity=24.1228070175439, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24654424, Length=456, Percent_Identity=24.1228070175439, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24641187, Length=470, Percent_Identity=24.2553191489362, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922444, Length=247, Percent_Identity=27.5303643724696, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 68073; Mature: 67942

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEPVNITILGTSDLHGTFVPWDYSTDTANLAGSLSQIATQVHQVRAQQPNVILVDAGD
CCCCCEEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
TIQGNFVETFKNDKTSPMILGFNALDYDVWVMGNHEFDFGLKPLSTSLNQFKGTALAGNI
CCCCCHHHHHCCCCCCCEEEEEEEECEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEE
LWDSGKPYLPAYKIIERQGVKIGIIGMDTPMTAEFAKGTDRVKGLNFTDPVQAVKQVIQQ
EECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHCCCHHHCCCCCCCHHHHHHHHHHH
IQGQVDAIVLVAHMGIDNENQRPGTGVGDIARANPELAAIVAGHMHVKVDKEVINGVIVT
HCCCCCEEEEEEEECCCCCCCCCCCCCCCHHCCCCCEEEEEECCEEEEEEHHHHCCEEEE
EPDKYGRALSRIDLQFEQHNGKYVLINKDSYTYPIKGIASDKKLEEIYQPYHKILRANAN
CCHHHHHHHHHEEEEEEECCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCC
RPIAQLSGQDLVPPDAVKGIPQVHVQDTGISKLYQEAGHYYAPQAQVIALQIDNDRPKLN
CCHHHCCCCCCCCCHHHCCCCEEEEECCCHHHHHHHCCCEECCCEEEEEEEECCCCCEEE
VGTITAKDIAFNYQYAGGEITVYQLSGKELKKYMEWSAGYFNQLHDGDVTYSFNPQRRAS
EEEEEEEHEEEEEEECCCEEEEEEECCHHHHHHHHHCCHHHHCCCCCCEEEEECCHHHHC
KYSTNDFFDGVTYKIDLRQPAGQRIRDLKMADGTPVTDSTPIRLGMNSYRMGHLTQKGGA
CCCCCCCCCCEEEEEECCCCCCCHHHHEECCCCCCCCCCCCEEECCCCCEECCEECCCCC
LEGMQFPVLSDSKVEYGEEAGTIRNLTIRYLTEVKKGQYQGQAVQRWQLVGLEGYERERK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHH
IVEKLLNDGKISVPTSEDGRYGNVASFNVKALLIGEPQARQQQQAALKAQLSAASDPLVK
HHHHHHCCCEEECCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHH
KRLSDQLVIIEAINKA
HHCCCCEEEEEEECCC
>Mature Secondary Structure 
ASEPVNITILGTSDLHGTFVPWDYSTDTANLAGSLSQIATQVHQVRAQQPNVILVDAGD
CCCCEEEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
TIQGNFVETFKNDKTSPMILGFNALDYDVWVMGNHEFDFGLKPLSTSLNQFKGTALAGNI
CCCCCHHHHHCCCCCCCEEEEEEEECEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEE
LWDSGKPYLPAYKIIERQGVKIGIIGMDTPMTAEFAKGTDRVKGLNFTDPVQAVKQVIQQ
EECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHCCCHHHCCCCCCCHHHHHHHHHHH
IQGQVDAIVLVAHMGIDNENQRPGTGVGDIARANPELAAIVAGHMHVKVDKEVINGVIVT
HCCCCCEEEEEEEECCCCCCCCCCCCCCCHHCCCCCEEEEEECCEEEEEEHHHHCCEEEE
EPDKYGRALSRIDLQFEQHNGKYVLINKDSYTYPIKGIASDKKLEEIYQPYHKILRANAN
CCHHHHHHHHHEEEEEEECCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCC
RPIAQLSGQDLVPPDAVKGIPQVHVQDTGISKLYQEAGHYYAPQAQVIALQIDNDRPKLN
CCHHHCCCCCCCCCHHHCCCCEEEEECCCHHHHHHHCCCEECCCEEEEEEEECCCCCEEE
VGTITAKDIAFNYQYAGGEITVYQLSGKELKKYMEWSAGYFNQLHDGDVTYSFNPQRRAS
EEEEEEEHEEEEEEECCCEEEEEEECCHHHHHHHHHCCHHHHCCCCCCEEEEECCHHHHC
KYSTNDFFDGVTYKIDLRQPAGQRIRDLKMADGTPVTDSTPIRLGMNSYRMGHLTQKGGA
CCCCCCCCCCEEEEEECCCCCCCHHHHEECCCCCCCCCCCCEEECCCCCEECCEECCCCC
LEGMQFPVLSDSKVEYGEEAGTIRNLTIRYLTEVKKGQYQGQAVQRWQLVGLEGYERERK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHH
IVEKLLNDGKISVPTSEDGRYGNVASFNVKALLIGEPQARQQQQAALKAQLSAASDPLVK
HHHHHHCCCEEECCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHH
KRLSDQLVIIEAINKA
HHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]