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The map label for this gene is cysG

Identifier: 157372830

GI number: 157372830

Start: 5073061

End: 5074464

Strand: Direct

Name: cysG

Synonym: Spro_4598

Alternate gene names: 157372830

Gene position: 5073061-5074464 (Clockwise)

Preceding gene: 157372829

Following gene: 157372843

Centisome position: 93.1

GC content: 60.9

Gene sequence:

>1404_bases
ATGGATTACCTGCCGATTTTCTGCCAACTGCAACACAAAGCCTGCCTGCTGGTCGGTGGCGGCGAGATTGCCGAGCGCAA
GGCTCGCCTGCTGCTGGACGCCGGTGCGGCACTGACCGTTAACGCCTGTAGCTTCACCCCACAGTTCCACGAATGGGCAG
CTCTTGGCCGCCTCACGCTGGCGGCCGGTGAATTCAGTGCAGAGTTACTGGCAGAAAAGTGGCTGGTGATCGCCGCCACC
GATCGAGTTGAGGTCAATGCGCTGGTGTACCAATGTGCCAACCAGCAGCGGGTATTTTGCAACGTGGTCGACGATCCGAA
GCGCGCCAGCTTTATCATGCCGTCGATTATCGATCGCTCGCCGATTATGGTCGCAGTGTCCTCCGGCGGTAAAGCCCCAG
TGCTGGCACGACTGCTGCGCGAAAAGCTGGAAGCCGTACTGCCCCAACATCTTGGCAAGTTGGCACAGTTGGGCGGTTCA
CTGCGTCAGCGGGTGAAAAAACACTTCAGCGACATCGGCTCACGCCGCCGTTTTTGGGAAAAGCTGTTTGCCCACGATCG
GCTGGCACAGTCACTGGCCAATAATGACGTAGCGCTGGCGGAGCGGCAAATTGAGCAACTGTTTAGCCATCAACCGCAAG
AGTGCGGCGAAGTGGTGCTGGTGGGGGCCGGGCCGGGCGATGCCGGGTTGCTGACGCTGAAAGGCCTGCAGCAAATCCAG
CAGGCTGACGTGGTGGTTTACGACCGGCTGGTGTCTGATGAAATCATGACACTGGTGCGCCGCGATGCCGAACGCATTTT
TGTCGGCAAACGGGCCGGCCACCATTGCGTGCCGCAGGAGCAGATCAACCAAATCCTGCTGCAGCAGGCGCAGCTCGGTA
AACGCGTGGTGCGGCTGAAAGGCGGTGACCCCTTTATCTTCGGCCGCGGTGGTGAAGAGCTGGAAACCCTGGCCGACGCC
AATATTCCGTTCTCGGTGGTGCCGGGCATTACCGCCGCCTCTGGTTGTTCGGCCTACAGCGGCATTCCCCTCACCCATCG
CGACCACGCCCAGAGCGTGCGGCTGGTGACCGGCCACGCCAAGAGTGACGGTGGGTTGGACTGGTCAACGCTGGCTGCCG
GGCAGCAGACGCTGGTGTTTTACATGGGCCTGACGCAGGCGGCGGACATCCAACGCCAACTGATCGCCCACGGCATGCCT
GCCGCTACGCCGGTGGCGTTGGTAGAAAACGGCACTTCCTGCCGCCAGCGAGTGATCGAAGGGGAATTAAGCCAATTGGG
GACGCTGGCCTTACAGGCTGCCAGCCCAAGTTTAATCATCGTCGGTAGCGTAGTCAGCCTGCGCAGCAAGCTGAACTGGT
TCTCAAGCCAGGAGCCGTCGCAACCACTGGCGCAAATGGCCTGA

Upstream 100 bases:

>100_bases
CGCGCCGAACGGCCACAGGCTGAGAAAACCGTAGCACGCCAGACCGCATAATATTCCGGGGCGCCTTGCGCCCCACTGCT
GATTGCCCCGAGGATTGCCG

Downstream 100 bases:

>100_bases
TACAGACGTAAAAAAAGCGGCCAGTGGCCGCTTTTCTTTTTGCTCGGAGCTTCACTGCGGGGAAACAAAGCCCACCGCCT
GGTAAACCTTCGCCAGCGTT

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MDYLPIFCQLQHKACLLVGGGEIAERKARLLLDAGAALTVNACSFTPQFHEWAALGRLTLAAGEFSAELLAEKWLVIAAT
DRVEVNALVYQCANQQRVFCNVVDDPKRASFIMPSIIDRSPIMVAVSSGGKAPVLARLLREKLEAVLPQHLGKLAQLGGS
LRQRVKKHFSDIGSRRRFWEKLFAHDRLAQSLANNDVALAERQIEQLFSHQPQECGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDEIMTLVRRDAERIFVGKRAGHHCVPQEQINQILLQQAQLGKRVVRLKGGDPFIFGRGGEELETLADA
NIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKSDGGLDWSTLAAGQQTLVFYMGLTQAADIQRQLIAHGMP
AATPVALVENGTSCRQRVIEGELSQLGTLALQAASPSLIIVGSVVSLRSKLNWFSSQEPSQPLAQMA

Sequences:

>Translated_467_residues
MDYLPIFCQLQHKACLLVGGGEIAERKARLLLDAGAALTVNACSFTPQFHEWAALGRLTLAAGEFSAELLAEKWLVIAAT
DRVEVNALVYQCANQQRVFCNVVDDPKRASFIMPSIIDRSPIMVAVSSGGKAPVLARLLREKLEAVLPQHLGKLAQLGGS
LRQRVKKHFSDIGSRRRFWEKLFAHDRLAQSLANNDVALAERQIEQLFSHQPQECGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDEIMTLVRRDAERIFVGKRAGHHCVPQEQINQILLQQAQLGKRVVRLKGGDPFIFGRGGEELETLADA
NIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKSDGGLDWSTLAAGQQTLVFYMGLTQAADIQRQLIAHGMP
AATPVALVENGTSCRQRVIEGELSQLGTLALQAASPSLIIVGSVVSLRSKLNWFSSQEPSQPLAQMA
>Mature_467_residues
MDYLPIFCQLQHKACLLVGGGEIAERKARLLLDAGAALTVNACSFTPQFHEWAALGRLTLAAGEFSAELLAEKWLVIAAT
DRVEVNALVYQCANQQRVFCNVVDDPKRASFIMPSIIDRSPIMVAVSSGGKAPVLARLLREKLEAVLPQHLGKLAQLGGS
LRQRVKKHFSDIGSRRRFWEKLFAHDRLAQSLANNDVALAERQIEQLFSHQPQECGEVVLVGAGPGDAGLLTLKGLQQIQ
QADVVVYDRLVSDEIMTLVRRDAERIFVGKRAGHHCVPQEQINQILLQQAQLGKRVVRLKGGDPFIFGRGGEELETLADA
NIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHAKSDGGLDWSTLAAGQQTLVFYMGLTQAADIQRQLIAHGMP
AATPVALVENGTSCRQRVIEGELSQLGTLALQAASPSLIIVGSVVSLRSKLNWFSSQEPSQPLAQMA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=457, Percent_Identity=72.4288840262582, Blast_Score=672, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322922, Length=237, Percent_Identity=34.5991561181435, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG2_SERP5 (A8GKQ1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480819.1
- ProteinModelPortal:   A8GKQ1
- SMR:   A8GKQ1
- STRING:   A8GKQ1
- GeneID:   5606867
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4598
- eggNOG:   COG0007
- HOGENOM:   HBG730212
- OMA:   MCRRDAE
- ProtClustDB:   PRK10637
- BioCyc:   SPRO399741:SPRO_4598-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 50575; Mature: 50575

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLPIFCQLQHKACLLVGGGEIAERKARLLLDAGAALTVNACSFTPQFHEWAALGRLTL
CCCCCEEEEECCCEEEEECCCHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHEE
AAGEFSAELLAEKWLVIAATDRVEVNALVYQCANQQRVFCNVVDDPKRASFIMPSIIDRS
ECCHHHHHHHHHCEEEEEECCCEEHHHHHHHHCCCCEEEEEECCCCCHHHHHCHHHCCCC
PIMVAVSSGGKAPVLARLLREKLEAVLPQHLGKLAQLGGSLRQRVKKHFSDIGSRRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFAHDRLAQSLANNDVALAERQIEQLFSHQPQECGEVVLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVVVYDRLVSDEIMTLVRRDAERIFVGKRAGHHCVPQEQINQILLQQAQLGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
GGDPFIFGRGGEELETLADANIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHA
CCCEEEECCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KSDGGLDWSTLAAGQQTLVFYMGLTQAADIQRQLIAHGMPAATPVALVENGTSCRQRVIE
CCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHEECCCHHHHHHHHH
GELSQLGTLALQAASPSLIIVGSVVSLRSKLNWFSSQEPSQPLAQMA
HHHHHHHHHHEECCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure
MDYLPIFCQLQHKACLLVGGGEIAERKARLLLDAGAALTVNACSFTPQFHEWAALGRLTL
CCCCCEEEEECCCEEEEECCCHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHEE
AAGEFSAELLAEKWLVIAATDRVEVNALVYQCANQQRVFCNVVDDPKRASFIMPSIIDRS
ECCHHHHHHHHHCEEEEEECCCEEHHHHHHHHCCCCEEEEEECCCCCHHHHHCHHHCCCC
PIMVAVSSGGKAPVLARLLREKLEAVLPQHLGKLAQLGGSLRQRVKKHFSDIGSRRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLFAHDRLAQSLANNDVALAERQIEQLFSHQPQECGEVVLVGAGPGDAGLLTLKGLQQIQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEHHHHHHHH
QADVVVYDRLVSDEIMTLVRRDAERIFVGKRAGHHCVPQEQINQILLQQAQLGKRVVRLK
HCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
GGDPFIFGRGGEELETLADANIPFSVVPGITAASGCSAYSGIPLTHRDHAQSVRLVTGHA
CCCEEEECCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
KSDGGLDWSTLAAGQQTLVFYMGLTQAADIQRQLIAHGMPAATPVALVENGTSCRQRVIE
CCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHEECCCHHHHHHHHH
GELSQLGTLALQAASPSLIIVGSVVSLRSKLNWFSSQEPSQPLAQMA
HHHHHHHHHHEECCCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA