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The map label for this gene is tusD

Identifier: 157372786

GI number: 157372786

Start: 5029752

End: 5030141

Strand: Reverse

Name: tusD

Synonym: Spro_4554

Alternate gene names: 157372786

Gene position: 5030141-5029752 (Counterclockwise)

Preceding gene: 157372787

Following gene: 157372785

Centisome position: 92.32

GC content: 60.0

Gene sequence:

>390_bases
ATGCTGGATTACTGCCTTGTGGTCACCGGTCCGGCGTATGGCACCCAGCAGGCCAGTAGCGCCTATCAGTTTGCTCAGGC
TCTATTGGCGAAAGGACACCGCCTCTCCAGCGTATTTTTTTACCGTGAAGGGGTGTTGAACGCCAACCAGTTGACGGCAC
CAGCCGGTGATGAGTTTGATTTGGTCCGTGGCTGGGTACAATTGGCGCGGCAGCATGGCATCGAGCTCAATGTCTGCGTT
GCCGCTGCGTTGCGACGTGGGGTGACCGATGAGCAGGAGGCGGCACAACAGGGGTTGCCCGCGGCAAACCTCCAACCGGG
TTTTATCCTCAGCGGGTTGGGCTCACTGGCCGAGGCTGCGCTGAGCAGTGACCGCCTGGTGCAGTTCTGA

Upstream 100 bases:

>100_bases
CGATTAATCAGGTGGCGGATCGTCTGAATATCTCCAAGCACACCGTCTATCTGTATATCCGCCAGTTCAAAAGCGGCGAT
CTGTTGGGAACTGAACGCTG

Downstream 100 bases:

>100_bases
GAGAATTTATGAAACGAGTTGCTTTTGTTTTTACCCAGGGACCTCACGGCAACGCCGGCGGCAGGGAAGGGCTGGATGCG
TTACTGGCCACCTCGGCGCT

Product: sulfur transfer complex subunit TusD

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MLDYCLVVTGPAYGTQQASSAYQFAQALLAKGHRLSSVFFYREGVLNANQLTAPAGDEFDLVRGWVQLARQHGIELNVCV
AAALRRGVTDEQEAAQQGLPAANLQPGFILSGLGSLAEAALSSDRLVQF

Sequences:

>Translated_129_residues
MLDYCLVVTGPAYGTQQASSAYQFAQALLAKGHRLSSVFFYREGVLNANQLTAPAGDEFDLVRGWVQLARQHGIELNVCV
AAALRRGVTDEQEAAQQGLPAANLQPGFILSGLGSLAEAALSSDRLVQF
>Mature_129_residues
MLDYCLVVTGPAYGTQQASSAYQFAQALLAKGHRLSSVFFYREGVLNANQLTAPAGDEFDLVRGWVQLARQHGIELNVCV
AAALRRGVTDEQEAAQQGLPAANLQPGFILSGLGSLAEAALSSDRLVQF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family

Homologues:

Organism=Escherichia coli, GI1789743, Length=128, Percent_Identity=73.4375, Blast_Score=196, Evalue=5e-52,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): TUSD_SERP5 (A8GKK7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480775.1
- ProteinModelPortal:   A8GKK7
- SMR:   A8GKK7
- STRING:   A8GKK7
- GeneID:   5604662
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4554
- eggNOG:   COG1553
- HOGENOM:   HBG538485
- OMA:   QDGVYNS
- ProtClustDB:   PRK00207
- BioCyc:   SPRO399741:SPRO_4554-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00390
- InterPro:   IPR003787
- InterPro:   IPR017463
- TIGRFAMs:   TIGR03012

Pfam domain/function: PF02635 DrsE

EC number: NA

Molecular weight: Translated: 13768; Mature: 13768

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: ACT_SITE 79-79

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYCLVVTGPAYGTQQASSAYQFAQALLAKGHRLSSVFFYREGVLNANQLTAPAGDEFD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHH
LVRGWVQLARQHGIELNVCVAAALRRGVTDEQEAAQQGLPAANLQPGFILSGLGSLAEAA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LSSDRLVQF
HCCCCCCCC
>Mature Secondary Structure
MLDYCLVVTGPAYGTQQASSAYQFAQALLAKGHRLSSVFFYREGVLNANQLTAPAGDEFD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHH
LVRGWVQLARQHGIELNVCVAAALRRGVTDEQEAAQQGLPAANLQPGFILSGLGSLAEAA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
LSSDRLVQF
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA