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The map label for this gene is tusB

Identifier: 157372784

GI number: 157372784

Start: 5029087

End: 5029374

Strand: Reverse

Name: tusB

Synonym: Spro_4552

Alternate gene names: 157372784

Gene position: 5029374-5029087 (Counterclockwise)

Preceding gene: 157372785

Following gene: 157372783

Centisome position: 92.3

GC content: 52.78

Gene sequence:

>288_bases
ATGCTGTATACCCTTAGCCGTTCCCCCACTCAATGCGATTTGCCTGCACTGCTGCGCCTGACGGCAGCGGGCGACGACCT
GCTGCTGCTACAGGATGGTGTGCTGGCCGGCCTGGCCGGTAGTGCGCATCTTGAATTGCTGCTTAATGCCCCCATATCCC
TTTATGCACTGCAAGATGACCTGGAAGCCAGAGGCTTGACTGGTCATTTTTCACACAAAATCACTGTCATCGGCTATAAT
CACTTCGTTGAATTAACCGAAAAGCATCGCAGTCAAATGTCCTGGTGA

Upstream 100 bases:

>100_bases
GGTAACTGACTGGGTGCTGGATGCCGAGGTATTGGCACCGGACGCGCTGGGCCAACTATTGGCCGGCTATGATGCGGTGA
TGACTTTTTAGGAAATCCTG

Downstream 100 bases:

>100_bases
TGAGAGTAATGTTGTATATTTCTTGACACCTCTACTGCTCAGCCATAAAATTCTGCGTCCCGTACTTTGCCTGTAGTGCA
TACGGGGGGATTTATCACAT

Product: sulfur transfer complex subunit TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MLYTLSRSPTQCDLPALLRLTAAGDDLLLLQDGVLAGLAGSAHLELLLNAPISLYALQDDLEARGLTGHFSHKITVIGYN
HFVELTEKHRSQMSW

Sequences:

>Translated_95_residues
MLYTLSRSPTQCDLPALLRLTAAGDDLLLLQDGVLAGLAGSAHLELLLNAPISLYALQDDLEARGLTGHFSHKITVIGYN
HFVELTEKHRSQMSW
>Mature_95_residues
MLYTLSRSPTQCDLPALLRLTAAGDDLLLLQDGVLAGLAGSAHLELLLNAPISLYALQDDLEARGLTGHFSHKITVIGYN
HFVELTEKHRSQMSW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family

Homologues:

Organism=Escherichia coli, GI1789741, Length=95, Percent_Identity=60, Blast_Score=109, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSB_SERP5 (A8GKK5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480773.1
- ProteinModelPortal:   A8GKK5
- SMR:   A8GKK5
- STRING:   A8GKK5
- GeneID:   5604660
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_4552
- eggNOG:   COG2168
- HOGENOM:   HBG682569
- OMA:   SPWHADI
- ProtClustDB:   PRK13510
- BioCyc:   SPRO399741:SPRO_4552-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01564
- InterPro:   IPR007215
- TIGRFAMs:   TIGR03011

Pfam domain/function: PF04077 DsrH

EC number: NA

Molecular weight: Translated: 10428; Mature: 10428

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYTLSRSPTQCDLPALLRLTAAGDDLLLLQDGVLAGLAGSAHLELLLNAPISLYALQDD
CEEECCCCCCCCCCHHHEEEECCCCCEEEEECCEEEECCCCCEEEEEECCCEEEEEECCC
LEARGLTGHFSHKITVIGYNHFVELTEKHRSQMSW
CCCCCCCCCCCCEEEEEECCHHEEHHHHHHHCCCC
>Mature Secondary Structure
MLYTLSRSPTQCDLPALLRLTAAGDDLLLLQDGVLAGLAGSAHLELLLNAPISLYALQDD
CEEECCCCCCCCCCHHHEEEECCCCCEEEEECCEEEECCCCCEEEEEECCCEEEEEECCC
LEARGLTGHFSHKITVIGYNHFVELTEKHRSQMSW
CCCCCCCCCCCCEEEEEECCHHEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA