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The map label for this gene is mltF [H]

Identifier: 157372671

GI number: 157372671

Start: 4910068

End: 4911618

Strand: Direct

Name: mltF [H]

Synonym: Spro_4438

Alternate gene names: 157372671

Gene position: 4910068-4911618 (Clockwise)

Preceding gene: 157372670

Following gene: 157372673

Centisome position: 90.11

GC content: 54.55

Gene sequence:

>1551_bases
ATGAATGCCACCTCTTCCCAAAGATTGCTAGTCGCCTGCCTGGCGTGTCTCACAGCCCTGCTGTTCACCGGTTCCGTATA
TGCGGCCCCAATGAAGGACAAGACGCAGGATCAGGAAGAACCAGAGACGCTCCAGGTCAATATGGACGACATCATGCGCC
CCTGGCTGGGGGATTTGCCAGGCATGCTCGACCGACGGGTGATTCGGGTATTGACGACTTACAGCAAAACCTTTTTCTTT
ATCACCAAGGGCACCCAGCGCGGAGCCACCCATGACATATTTATGGAGTTCGAGCACGACCTTAATCTCAAGTTGATGAA
GGATAAAAAGCTCAAACAGCGCCATCTTAAGGTGCGCGTCATCTTCGTGCCGGTAGCTCGTGACCAGTTACTCAATGCCC
TCAATGAAGGCCGGGGCGACATTGCTGCCTCCAACCTCACCATTACCCCGGCGCGTCAGGAGCAAGTGACGTTCACCACC
GCCCTTTATCCGAAAGTGAAGGAACTGCTGATTTCTGGCCCCTCCTCACCCAAAGTGGAAAACCTGGAACAACTTTCCGG
AAAAACGGTATTCGTGCGCCTCTCATCGAGCTATCACGAAAGTCTGGTGGCCCTGAATAAGCGCTTTGCCCAGGAATCAC
GTCCTCCCGTTATCCTGGAAACAGCCCCCGAAGCGCTCGAGGATGAGGATCTGATCGAGATGCTGAGTGCCGGCCTCATT
CCACTTATCGTGGTTGATCGCCATAAGGCTCTGTTCTGGAAACAGGTTTTCCCTAAAATTCAGATACACGAAAATGTGGT
GCTGCGTGACGATGCCGACATCGCCTGGGCGGTGCGCAAGGATAACCCGCAGTTACTCGCTTTGCTCAACGGCTTCGTAG
AGAAGAATCGCCAGGGCAGCAAGTTGGGTAACACCCTTCTGTTGCGCTACCTGAAAAATGCCAAATATGTCAAAAACGCG
GCCTCGGAGAAAGAGCGCGCCAAATTTCTGGCGATGGTGGACGTGTTCAGGAAATATGGCGATCGCTATAACGTGGACTG
GCTACTGATGGTGGCCCAGGGCTATCAGGAGTCGCGCCTCAACCAGTCGGTCCGCAGCCCGGTGGGTGCTATCGGTATTA
TGCAGGTGATGCCGGGCACCGGCAAAGAGCTGAAAGTCGGCGACATCCGCAAACTGGATCCCAATATCAATGCCGGGGTG
AAATACATGCGCTGGATGATTGACCGCTACTACGCAGACGAGCCGATGACGCCCCTCGACAAAGTGCTGTTCTCATTCGC
CTCTTACAACGCAGGCCCAGCCCGCATCGCTCGTTTGCGAGCGGAAACGGAGAAACGTGGCTTTAGCCCCAATATCTGGT
TTGGCAATGTGGAATACCTGGCCGCCGAACGGATTGGGGCTGAGACGGTGACCTATGTCAGCAATATCTACAAATACTAC
GTGGCTTATCGGCTGATCATGGATCAGCGAACTCGCAAGCAGCAGGCCATGGACCAATCCAAAAGTGCGCCAGCAGCAGA
GAAGCCGCAACAAGCCCCAGTGGGGCAGTGA

Upstream 100 bases:

>100_bases
AAGGAGAGGGAACAATATCACCTTAACTGACCAGCATTGCCCTCTCGGGGCTTTTTTAGTTTGACTACAATGAGAAGACG
CACCATTCGGAGTGTCTTCC

Downstream 100 bases:

>100_bases
GTTTGGCGTGAAGCAGCCTCCTTCAGAAACAAAAAAGCCCCTTTCGGGGCTTTTTCATCGGCTTTAGACAACCTGATATC
AGAAAGGAATATCGTCGTCG

Product: lytic transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MNATSSQRLLVACLACLTALLFTGSVYAAPMKDKTQDQEEPETLQVNMDDIMRPWLGDLPGMLDRRVIRVLTTYSKTFFF
ITKGTQRGATHDIFMEFEHDLNLKLMKDKKLKQRHLKVRVIFVPVARDQLLNALNEGRGDIAASNLTITPARQEQVTFTT
ALYPKVKELLISGPSSPKVENLEQLSGKTVFVRLSSSYHESLVALNKRFAQESRPPVILETAPEALEDEDLIEMLSAGLI
PLIVVDRHKALFWKQVFPKIQIHENVVLRDDADIAWAVRKDNPQLLALLNGFVEKNRQGSKLGNTLLLRYLKNAKYVKNA
ASEKERAKFLAMVDVFRKYGDRYNVDWLLMVAQGYQESRLNQSVRSPVGAIGIMQVMPGTGKELKVGDIRKLDPNINAGV
KYMRWMIDRYYADEPMTPLDKVLFSFASYNAGPARIARLRAETEKRGFSPNIWFGNVEYLAAERIGAETVTYVSNIYKYY
VAYRLIMDQRTRKQQAMDQSKSAPAAEKPQQAPVGQ

Sequences:

>Translated_516_residues
MNATSSQRLLVACLACLTALLFTGSVYAAPMKDKTQDQEEPETLQVNMDDIMRPWLGDLPGMLDRRVIRVLTTYSKTFFF
ITKGTQRGATHDIFMEFEHDLNLKLMKDKKLKQRHLKVRVIFVPVARDQLLNALNEGRGDIAASNLTITPARQEQVTFTT
ALYPKVKELLISGPSSPKVENLEQLSGKTVFVRLSSSYHESLVALNKRFAQESRPPVILETAPEALEDEDLIEMLSAGLI
PLIVVDRHKALFWKQVFPKIQIHENVVLRDDADIAWAVRKDNPQLLALLNGFVEKNRQGSKLGNTLLLRYLKNAKYVKNA
ASEKERAKFLAMVDVFRKYGDRYNVDWLLMVAQGYQESRLNQSVRSPVGAIGIMQVMPGTGKELKVGDIRKLDPNINAGV
KYMRWMIDRYYADEPMTPLDKVLFSFASYNAGPARIARLRAETEKRGFSPNIWFGNVEYLAAERIGAETVTYVSNIYKYY
VAYRLIMDQRTRKQQAMDQSKSAPAAEKPQQAPVGQ
>Mature_516_residues
MNATSSQRLLVACLACLTALLFTGSVYAAPMKDKTQDQEEPETLQVNMDDIMRPWLGDLPGMLDRRVIRVLTTYSKTFFF
ITKGTQRGATHDIFMEFEHDLNLKLMKDKKLKQRHLKVRVIFVPVARDQLLNALNEGRGDIAASNLTITPARQEQVTFTT
ALYPKVKELLISGPSSPKVENLEQLSGKTVFVRLSSSYHESLVALNKRFAQESRPPVILETAPEALEDEDLIEMLSAGLI
PLIVVDRHKALFWKQVFPKIQIHENVVLRDDADIAWAVRKDNPQLLALLNGFVEKNRQGSKLGNTLLLRYLKNAKYVKNA
ASEKERAKFLAMVDVFRKYGDRYNVDWLLMVAQGYQESRLNQSVRSPVGAIGIMQVMPGTGKELKVGDIRKLDPNINAGV
KYMRWMIDRYYADEPMTPLDKVLFSFASYNAGPARIARLRAETEKRGFSPNIWFGNVEYLAAERIGAETVTYVSNIYKYY
VAYRLIMDQRTRKQQAMDQSKSAPAAEKPQQAPVGQ

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=483, Percent_Identity=21.9461697722567, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082441, Length=178, Percent_Identity=32.5842696629214, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 58558; Mature: 58558

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATSSQRLLVACLACLTALLFTGSVYAAPMKDKTQDQEEPETLQVNMDDIMRPWLGDLP
CCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHCCC
GMLDRRVIRVLTTYSKTFFFITKGTQRGATHDIFMEFEHDLNLKLMKDKKLKQRHLKVRV
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHCEEEEE
IFVPVARDQLLNALNEGRGDIAASNLTITPARQEQVTFTTALYPKVKELLISGPSSPKVE
EEEECCHHHHHHHHHCCCCCEEECCEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCHH
NLEQLSGKTVFVRLSSSYHESLVALNKRFAQESRPPVILETAPEALEDEDLIEMLSAGLI
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCE
PLIVVDRHKALFWKQVFPKIQIHENVVLRDDADIAWAVRKDNPQLLALLNGFVEKNRQGS
EEEEECCHHHHHHHHHCCCEEECCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCH
KLGNTLLLRYLKNAKYVKNAASEKERAKFLAMVDVFRKYGDRYNVDWLLMVAQGYQESRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
NQSVRSPVGAIGIMQVMPGTGKELKVGDIRKLDPNINAGVKYMRWMIDRYYADEPMTPLD
HHHHHCCHHHHHHHHHCCCCCCEEECCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHH
KVLFSFASYNAGPARIARLRAETEKRGFSPNIWFGNVEYLAAERIGAETVTYVSNIYKYY
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHH
VAYRLIMDQRTRKQQAMDQSKSAPAAEKPQQAPVGQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNATSSQRLLVACLACLTALLFTGSVYAAPMKDKTQDQEEPETLQVNMDDIMRPWLGDLP
CCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHCCC
GMLDRRVIRVLTTYSKTFFFITKGTQRGATHDIFMEFEHDLNLKLMKDKKLKQRHLKVRV
HHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHCEEEEE
IFVPVARDQLLNALNEGRGDIAASNLTITPARQEQVTFTTALYPKVKELLISGPSSPKVE
EEEECCHHHHHHHHHCCCCCEEECCEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCHH
NLEQLSGKTVFVRLSSSYHESLVALNKRFAQESRPPVILETAPEALEDEDLIEMLSAGLI
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCE
PLIVVDRHKALFWKQVFPKIQIHENVVLRDDADIAWAVRKDNPQLLALLNGFVEKNRQGS
EEEEECCHHHHHHHHHCCCEEECCCEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCH
KLGNTLLLRYLKNAKYVKNAASEKERAKFLAMVDVFRKYGDRYNVDWLLMVAQGYQESRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
NQSVRSPVGAIGIMQVMPGTGKELKVGDIRKLDPNINAGVKYMRWMIDRYYADEPMTPLD
HHHHHCCHHHHHHHHHCCCCCCEEECCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHH
KVLFSFASYNAGPARIARLRAETEKRGFSPNIWFGNVEYLAAERIGAETVTYVSNIYKYY
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHH
VAYRLIMDQRTRKQQAMDQSKSAPAAEKPQQAPVGQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA