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The map label for this gene is dusB [H]

Identifier: 157372656

GI number: 157372656

Start: 4896787

End: 4897752

Strand: Direct

Name: dusB [H]

Synonym: Spro_4423

Alternate gene names: 157372656

Gene position: 4896787-4897752 (Clockwise)

Preceding gene: 157372655

Following gene: 157372657

Centisome position: 89.87

GC content: 55.59

Gene sequence:

>966_bases
ATGCGCATTGGACACCACCAGCTTACAAATTGCTTGATTGCGGCCCCGATGGCCGGCATTACAGATCGCCCATTTAGAGC
CCTATGTCATGCAATGGGTGCTGGGATGGCTGTATCCGAAATGCTCTCCTCCAATCCGGAGGTGTGGCGGACGGATAAGT
CGCGTCTGCGTATGGTCCATAGCGATGAACCAGGGATCCGTTCCGTGCAGATTGCCGGATGCGATCCGGATGATATGGCC
GCCGCGGCACGTATTAACGTGGCTGGTGGCGCACAGATCATCGACATCAATATGGGGTGCCCGGCCAAGAAAGTGAACCG
GAAGCTTGCAGGGTCTGCATTGCTGCAGTACCCGGATCTGGTTAAACAGATCCTCCACGCGGTAGTTAACGCTGTGGATG
TGCCGGTAACACTTAAGATCCGTACCGGCTGGGAACCCGAACACCGTAACTGTGTAGAGATTGCCCAACTGGCCGAAGAC
TGTGGTATACAGGCCCTGACTATTCATGGCCGAACCCGCGCCTGCCTGTTCAACGGCAACGCGGAGTACGACAGCATTCG
GGCAGTTAAGCAGAGTGTTTCCATTCCGATTATCGCGAATGGCGACATTACTGACCCGCATAAAGCCAGAGCAGTGCTCG
ACTACACTGGGGCTGATGCCCTGATGATAGGCCGCGCTGCTCAGGGGAGACCCTGGATCTTCCGGGAAATCCAGCATTAT
CTGGACACTGGGGAACTGCTGCCCCCTCCGCCACTTGGCGAGGTTAAGCGTTTGTTGATAGGGCATATTCGGGAATTGCA
CGGCTTTTACGGCCAAGGCAAGGGATTCCGGATTGCTCGTAAGCACGTATCCTGGTATCTCCAGGAGCACGCCCCGAATG
ACCAGTTTCGGCGCACATTCAACGCCATAGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATATTTCGAAAATCTT
GCGTAA

Upstream 100 bases:

>100_bases
GCCGAATAGCGCCTGATTGGTCAAAGTTTGGCCTTTCATCTCAACGCAAAAATGCGTAATATACGCGCCCTTGCGGACAC
AGTATGGTTACTCTTTGTCT

Downstream 100 bases:

>100_bases
CAAAAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTGAACTCACAGGA
CCAAGTGACTCAAAAGCCTC

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRIGHHQLTNCLIAAPMAGITDRPFRALCHAMGAGMAVSEMLSSNPEVWRTDKSRLRMVHSDEPGIRSVQIAGCDPDDMA
AAARINVAGGAQIIDINMGCPAKKVNRKLAGSALLQYPDLVKQILHAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAED
CGIQALTIHGRTRACLFNGNAEYDSIRAVKQSVSIPIIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPPPLGEVKRLLIGHIRELHGFYGQGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENL
A

Sequences:

>Translated_321_residues
MRIGHHQLTNCLIAAPMAGITDRPFRALCHAMGAGMAVSEMLSSNPEVWRTDKSRLRMVHSDEPGIRSVQIAGCDPDDMA
AAARINVAGGAQIIDINMGCPAKKVNRKLAGSALLQYPDLVKQILHAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAED
CGIQALTIHGRTRACLFNGNAEYDSIRAVKQSVSIPIIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPPPLGEVKRLLIGHIRELHGFYGQGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENL
A
>Mature_321_residues
MRIGHHQLTNCLIAAPMAGITDRPFRALCHAMGAGMAVSEMLSSNPEVWRTDKSRLRMVHSDEPGIRSVQIAGCDPDDMA
AAARINVAGGAQIIDINMGCPAKKVNRKLAGSALLQYPDLVKQILHAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAED
CGIQALTIHGRTRACLFNGNAEYDSIRAVKQSVSIPIIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPPPLGEVKRLLIGHIRELHGFYGQGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENL
A

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=235, Percent_Identity=32.3404255319149, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI239788462, Length=235, Percent_Identity=32.3404255319149, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI31742496, Length=318, Percent_Identity=28.3018867924528, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=30.7017543859649, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI8923374, Length=254, Percent_Identity=31.1023622047244, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=87.2274143302181, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1788462, Length=253, Percent_Identity=31.6205533596838, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693211, Length=297, Percent_Identity=23.5690235690236, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=244, Percent_Identity=29.9180327868852, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=33.1838565022422, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=234, Percent_Identity=32.0512820512821, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=31.1740890688259, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=262, Percent_Identity=29.7709923664122, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.4372294372294, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=29.8245614035088, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=28.2051282051282, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24580595, Length=268, Percent_Identity=30.2238805970149, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920448, Length=268, Percent_Identity=30.2238805970149, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=254, Percent_Identity=31.1023622047244, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921524, Length=312, Percent_Identity=25.6410256410256, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 35388; Mature: 35388

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHHQLTNCLIAAPMAGITDRPFRALCHAMGAGMAVSEMLSSNPEVWRTDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHEEEEE
SDEPGIRSVQIAGCDPDDMAAAARINVAGGAQIIDINMGCPAKKVNRKLAGSALLQYPDL
CCCCCCCEEEEECCCCHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VKQILHAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAEDCGIQALTIHGRTRACLFNGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCC
AEYDSIRAVKQSVSIPIIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCHHHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPPPLGEVKRLLIGHIRELHGFYGQGKGFRIARKHVSWYLQEHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENLA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGHHQLTNCLIAAPMAGITDRPFRALCHAMGAGMAVSEMLSSNPEVWRTDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEECCHHHEEEEE
SDEPGIRSVQIAGCDPDDMAAAARINVAGGAQIIDINMGCPAKKVNRKLAGSALLQYPDL
CCCCCCCEEEEECCCCHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VKQILHAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAEDCGIQALTIHGRTRACLFNGN
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEEEEECCC
AEYDSIRAVKQSVSIPIIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCHHHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPPPLGEVKRLLIGHIRELHGFYGQGKGFRIARKHVSWYLQEHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENLA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9811652 [H]