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The map label for this gene is rihA [H]

Identifier: 157372629

GI number: 157372629

Start: 4859112

End: 4860056

Strand: Direct

Name: rihA [H]

Synonym: Spro_4396

Alternate gene names: 157372629

Gene position: 4859112-4860056 (Clockwise)

Preceding gene: 157372626

Following gene: 157372646

Centisome position: 89.18

GC content: 60.63

Gene sequence:

>945_bases
ATGAAAAAAATCATACTGGACTGCGACCCGGGACATGACGATGCGATCGCCATGCTGCTGGCCTGGGGCAACCCGCAGAT
CGATCTGCTGGCCGTGACCACCGTGGTGGGCAACCAAACGCTGGAGAAGGTCACCCGTAACGCGCTGGCCGTCGCACGCA
TCGCCAATATTACCGGCGTACCCTTTGCCGCCGGCTGCCCGCGCCCGTTGGTTCGCCAGATAGAAGTCGCACCCGATATC
CATGGCGAATCCGGCCTGGATGGCCCGGTATTACCGGAACCAACGCTGACGCTGGAACCCTGCCACGCGGTAGATCTGAT
CATCGATACCATCATGGCTCACCCGCCGGGCAGCGTGACGCTGGTTCCGACCGGCGGCCTGACCAACATCGCCATGGCGG
TGCGCAAAGAACCACGCATTGCCGAACGAGTGAAAGAAGTGGTGCTGATGGGCGGCGGCTACCACGTGGGCAACTGGAGC
GCGGTGGCGGAATTTAATATCAAGATCGATCCGGAAGCCGCGCACATCGTGTTCAATGAAAAGTGGCCGCTGACCATGGT
CGGGCTGGATCTGACCCATCAGGCGCTCGCCACGCCAGAAGTCTGTGCCCGCATCGCCGCTATCCAAACCGGACCAGCCA
GGTTTGTCGGCGAACTGCTTGAGTTCTTTGGCAAGATGTATCAACAGGCGCAGGGCTTTAGCGCCCCGCCGGTACACGAT
CCTTGCGCGGTGGCCTATGTGATCGACCCCGAAGTGATGACGGTGCGTAAAGTCCCGGTGGATATCGAACTGACCGGCAC
CCTGACGCTGGGCATGACGGTAGCCGACTTCCGCGCCGCACCGCCGCCGGACTGCCACACTCAGGTGGCGGTGACGCTGG
ATCAGGATAAGTTTTGGGATTTGATAGTGGATGCGCTGGAGAGAATTGGGGAAGTGAAGGTTTAG

Upstream 100 bases:

>100_bases
CAACGCTTTGATGTGGTTAGCAAAAAGCATTATCGGTCATTACCTCGCCCACCATCGGTGCGCTATGCTGCGGGGACCTC
TCCCTGCATCAGGAATAACA

Downstream 100 bases:

>100_bases
GCTGCGGCTTGTAGGACAGGTAATGGTAAGGCGGTGGGCCGGGGGTGTTTGGATAGCCACCCGGCCTGGGTTTATCGGGG
GATAAACTAGCGTTTTAGTA

Product: purine nucleosidase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKIILDCDPGHDDAIAMLLAWGNPQIDLLAVTTVVGNQTLEKVTRNALAVARIANITGVPFAAGCPRPLVRQIEVAPDI
HGESGLDGPVLPEPTLTLEPCHAVDLIIDTIMAHPPGSVTLVPTGGLTNIAMAVRKEPRIAERVKEVVLMGGGYHVGNWS
AVAEFNIKIDPEAAHIVFNEKWPLTMVGLDLTHQALATPEVCARIAAIQTGPARFVGELLEFFGKMYQQAQGFSAPPVHD
PCAVAYVIDPEVMTVRKVPVDIELTGTLTLGMTVADFRAAPPPDCHTQVAVTLDQDKFWDLIVDALERIGEVKV

Sequences:

>Translated_314_residues
MKKIILDCDPGHDDAIAMLLAWGNPQIDLLAVTTVVGNQTLEKVTRNALAVARIANITGVPFAAGCPRPLVRQIEVAPDI
HGESGLDGPVLPEPTLTLEPCHAVDLIIDTIMAHPPGSVTLVPTGGLTNIAMAVRKEPRIAERVKEVVLMGGGYHVGNWS
AVAEFNIKIDPEAAHIVFNEKWPLTMVGLDLTHQALATPEVCARIAAIQTGPARFVGELLEFFGKMYQQAQGFSAPPVHD
PCAVAYVIDPEVMTVRKVPVDIELTGTLTLGMTVADFRAAPPPDCHTQVAVTLDQDKFWDLIVDALERIGEVKV
>Mature_314_residues
MKKIILDCDPGHDDAIAMLLAWGNPQIDLLAVTTVVGNQTLEKVTRNALAVARIANITGVPFAAGCPRPLVRQIEVAPDI
HGESGLDGPVLPEPTLTLEPCHAVDLIIDTIMAHPPGSVTLVPTGGLTNIAMAVRKEPRIAERVKEVVLMGGGYHVGNWS
AVAEFNIKIDPEAAHIVFNEKWPLTMVGLDLTHQALATPEVCARIAAIQTGPARFVGELLEFFGKMYQQAQGFSAPPVHD
PCAVAYVIDPEVMTVRKVPVDIELTGTLTLGMTVADFRAAPPPDCHTQVAVTLDQDKFWDLIVDALERIGEVKV

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=301, Percent_Identity=45.1827242524917, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1786871, Length=307, Percent_Identity=43.3224755700326, Blast_Score=253, Evalue=9e-69,
Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=36.6336633663366, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32565474, Length=265, Percent_Identity=33.5849056603774, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17565698, Length=316, Percent_Identity=27.8481012658228, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=326, Percent_Identity=36.1963190184049, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24641839, Length=306, Percent_Identity=29.7385620915033, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24641837, Length=198, Percent_Identity=33.8383838383838, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583915, Length=189, Percent_Identity=30.6878306878307, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 33741; Mature: 33741

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIILDCDPGHDDAIAMLLAWGNPQIDLLAVTTVVGNQTLEKVTRNALAVARIANITGV
CCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCC
PFAAGCPRPLVRQIEVAPDIHGESGLDGPVLPEPTLTLEPCHAVDLIIDTIMAHPPGSVT
CCCCCCCHHHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEE
LVPTGGLTNIAMAVRKEPRIAERVKEVVLMGGGYHVGNWSAVAEFNIKIDPEAAHIVFNE
EEECCCHHHHHHHHHCCCHHHHHHHHHEEECCCEEECCCEEEEEEEEEECCCEEEEEECC
KWPLTMVGLDLTHQALATPEVCARIAAIQTGPARFVGELLEFFGKMYQQAQGFSAPPVHD
CCCEEEEECCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PCAVAYVIDPEVMTVRKVPVDIELTGTLTLGMTVADFRAAPPPDCHTQVAVTLDQDKFWD
CEEEEEEECCCEEEEEECCEEEEEEEEEEECEEEECCCCCCCCCCCCEEEEEECCHHHHH
LIVDALERIGEVKV
HHHHHHHHHCCCCC
>Mature Secondary Structure
MKKIILDCDPGHDDAIAMLLAWGNPQIDLLAVTTVVGNQTLEKVTRNALAVARIANITGV
CCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEEHHCCHHHHHHHHHHHHHHHHHHCCCC
PFAAGCPRPLVRQIEVAPDIHGESGLDGPVLPEPTLTLEPCHAVDLIIDTIMAHPPGSVT
CCCCCCCHHHHHHEECCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEE
LVPTGGLTNIAMAVRKEPRIAERVKEVVLMGGGYHVGNWSAVAEFNIKIDPEAAHIVFNE
EEECCCHHHHHHHHHCCCHHHHHHHHHEEECCCEEECCCEEEEEEEEEECCCEEEEEECC
KWPLTMVGLDLTHQALATPEVCARIAAIQTGPARFVGELLEFFGKMYQQAQGFSAPPVHD
CCCEEEEECCHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PCAVAYVIDPEVMTVRKVPVDIELTGTLTLGMTVADFRAAPPPDCHTQVAVTLDQDKFWD
CEEEEEEECCCEEEEEECCEEEEEEEEEEECEEEECCCCCCCCCCCCEEEEEECCHHHHH
LIVDALERIGEVKV
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA