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The map label for this gene is gltD [H]

Identifier: 157372578

GI number: 157372578

Start: 4817818

End: 4819236

Strand: Direct

Name: gltD [H]

Synonym: Spro_4345

Alternate gene names: 157372578

Gene position: 4817818-4819236 (Clockwise)

Preceding gene: 157372577

Following gene: 157372584

Centisome position: 88.42

GC content: 58.28

Gene sequence:

>1419_bases
ATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTCAAGATCCGTAAAATTGA
GTTTGTAGAGATTTACGAGCCGTTTTCGGAAAGTCAGGCGAAGGCGCAGGCGGACCGTTGTCTGTCCTGCGGTAACCCTT
ATTGCGAATGGAAATGCCCGGTTCACAACTACATCCCGAACTGGCTGAAGCTGGCGAATGAAGGCCGCATCATGGAGGCA
GCGGATCTGGCGCACCAGACCAACAGCCTGCCGGAAGTGTGCGGCCGCGTCTGCCCGCAGGATCGTCTGTGTGAAGGTTC
CTGTACCCTGAACGACGAGTTCGGTGCGGTGACCATCGGCAACATCGAACGCTATATCAGCGATAAAGCGATTGAGATGG
GCTGGAAACCGGACATGTCTCACGTGCTGCCTACCGGTAAACGCGTGGCGGTGATCGGGGCCGGCCCGGCAGGCCTGGCC
TGTGCCGATGTGCTGACCCGTAACGGCGTGAAAGCGGTGGTTTACGATCGTCACCCGGAGATCGGCGGCCTGCTGACCTT
CGGGATCCCGGCCTTCAAGCTGGAAAAAGAAGTGATGGTGAAGCGCCGTGGCATCTTCAGCGAAATGGGCATTGAGTTCC
AGCTCAATACCGAAGTGGGCAAAGACATCACCATGGAGACGCTGCTGAGTGATTATGACGCGGTGTTCCTCGGCGTCGGT
ACCTATCAGTCAATGCGCGGTGGCCTGGAGAACGAGGATGCGCCGGGGGTCTATGACGCCCTGCCGTTCCTGATCGCCAA
CACCAAGCAGTTGATGGGCTATCAGGCCGAGCAGCACGAGCCTTATGTCAGCATGGAAGGCAAACGTGTGGTGGTGCTGG
GCGGTGGTGACACCGCGATGGACTGCGTGCGTACCTCTATTCGCCAGGGGGCAACCGACGTTATCTGCGCCTACCGCCGT
GATGAAGCCAACATGCCAGGCTCCAAACGCGAAGTGAAAAACGCGCGTGAGGAAGGGGTTGATTTCAAATTCAACCTGCA
GCCACTGAGCATCGAACTCAACAGCGCCGGTCGCGTGGCCGGAGTGAAAATGGTTCGTACCCAGTTGGGCGCACCGGATG
CCAACGGCCGCCAGATTGCTGAGCAGGTGCCAGGTTCCGAGCACATCATTGATGCCGACGCGGTGGTTATGGCCTTTGGT
TTCCGTCCGCACCAGATGGACTGGCTGGCCGCGCACGACGTGGAGCTCGACAAACAGGGCCGCATTGTGGCACCGGAAAG
CACCGATAATGCCTTCCAGACCAGCAACCCGAAAATCTTCGCCGGTGGCGATGCGGTGCGCGGGTCGGATCTGGTGGTAA
CGGCGATTGCCGAAGGTCGTAAAGCTGCCGATGGCATCATGAGCTACCTGGAAGTCTAA

Upstream 100 bases:

>100_bases
GCTCTGGTCAAGCCGAAGTCCAGTGATGTCAAAGCATTGTTGGGTCACCGTAGTCGAACCGCTGCCGAGCTGCGGGTTCA
GGCGCAGTAAGAGGTCATTA

Downstream 100 bases:

>100_bases
TCTTCCGCCGGCTTTTCCCCAAAGGGCCCGCAGGTGATCTGCCGGGCCCTTTGTTTTTTCCCGGATCGCAGGCAATAAAA
AAGGTCGCCTAAGCGACCTT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSESQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEA
ADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIEMGWKPDMSHVLPTGKRVAVIGAGPAGLA
CADVLTRNGVKAVVYDRHPEIGGLLTFGIPAFKLEKEVMVKRRGIFSEMGIEFQLNTEVGKDITMETLLSDYDAVFLGVG
TYQSMRGGLENEDAPGVYDALPFLIANTKQLMGYQAEQHEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATDVICAYRR
DEANMPGSKREVKNAREEGVDFKFNLQPLSIELNSAGRVAGVKMVRTQLGAPDANGRQIAEQVPGSEHIIDADAVVMAFG
FRPHQMDWLAAHDVELDKQGRIVAPESTDNAFQTSNPKIFAGGDAVRGSDLVVTAIAEGRKAADGIMSYLEV

Sequences:

>Translated_472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSESQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEA
ADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIEMGWKPDMSHVLPTGKRVAVIGAGPAGLA
CADVLTRNGVKAVVYDRHPEIGGLLTFGIPAFKLEKEVMVKRRGIFSEMGIEFQLNTEVGKDITMETLLSDYDAVFLGVG
TYQSMRGGLENEDAPGVYDALPFLIANTKQLMGYQAEQHEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATDVICAYRR
DEANMPGSKREVKNAREEGVDFKFNLQPLSIELNSAGRVAGVKMVRTQLGAPDANGRQIAEQVPGSEHIIDADAVVMAFG
FRPHQMDWLAAHDVELDKQGRIVAPESTDNAFQTSNPKIFAGGDAVRGSDLVVTAIAEGRKAADGIMSYLEV
>Mature_471_residues
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSESQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIMEAA
DLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYISDKAIEMGWKPDMSHVLPTGKRVAVIGAGPAGLAC
ADVLTRNGVKAVVYDRHPEIGGLLTFGIPAFKLEKEVMVKRRGIFSEMGIEFQLNTEVGKDITMETLLSDYDAVFLGVGT
YQSMRGGLENEDAPGVYDALPFLIANTKQLMGYQAEQHEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATDVICAYRRD
EANMPGSKREVKNAREEGVDFKFNLQPLSIELNSAGRVAGVKMVRTQLGAPDANGRQIAEQVPGSEHIIDADAVVMAFGF
RPHQMDWLAAHDVELDKQGRIVAPESTDNAFQTSNPKIFAGGDAVRGSDLVVTAIAEGRKAADGIMSYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=479, Percent_Identity=28.1837160751566, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=84.9576271186441, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=65.0759219088937, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=57.3333333333333, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=30.0904977375566, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789243, Length=422, Percent_Identity=30.5687203791469, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=32.5910931174089, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71984108, Length=468, Percent_Identity=30.7692307692308, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=169, Percent_Identity=28.4023668639053, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=484, Percent_Identity=34.0909090909091, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=37.8600823045267, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=37.8600823045267, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24665547, Length=486, Percent_Identity=37.8600823045267, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24665543, Length=486, Percent_Identity=37.8600823045267, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24640763, Length=460, Percent_Identity=28.0434782608696, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI18858217, Length=460, Percent_Identity=28.0434782608696, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 51768; Mature: 51636

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSESQAKAQADRCLSCGNPYCEWKCP
CCCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIMEAADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYISDKAIEMGWKPDMSHVLPTGKRVAVIGAGPAGLACADVLTRNGVKAVVYDRHPE
CHHHHHCCHHHCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMVKRRGIFSEMGIEFQLNTEVGKDITMETLLSDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCHHHHHHHCCCCEEEEECC
TYQSMRGGLENEDAPGVYDALPFLIANTKQLMGYQAEQHEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCHHCCCEEEECCCEEEEECCCCHHH
DCVRTSIRQGATDVICAYRRDEANMPGSKREVKNAREEGVDFKFNLQPLSIELNSAGRVA
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCHH
GVKMVRTQLGAPDANGRQIAEQVPGSEHIIDADAVVMAFGFRPHQMDWLAAHDVELDKQG
HHHHHHHHCCCCCCCCHHHHHHCCCCCEEECCCCEEEEECCCCCCCCEEEECCCCCCCCC
RIVAPESTDNAFQTSNPKIFAGGDAVRGSDLVVTAIAEGRKAADGIMSYLEV
CEECCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSESQAKAQADRCLSCGNPYCEWKCP
CCCHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIMEAADLAHQTNSLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYISDKAIEMGWKPDMSHVLPTGKRVAVIGAGPAGLACADVLTRNGVKAVVYDRHPE
CHHHHHCCHHHCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMVKRRGIFSEMGIEFQLNTEVGKDITMETLLSDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCHHHHHHHCCCCEEEEECC
TYQSMRGGLENEDAPGVYDALPFLIANTKQLMGYQAEQHEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCHHCCCEEEECCCEEEEECCCCHHH
DCVRTSIRQGATDVICAYRRDEANMPGSKREVKNAREEGVDFKFNLQPLSIELNSAGRVA
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEECCCCCHH
GVKMVRTQLGAPDANGRQIAEQVPGSEHIIDADAVVMAFGFRPHQMDWLAAHDVELDKQG
HHHHHHHHCCCCCCCCHHHHHHCCCCCEEECCCCEEEEECCCCCCCCEEEECCCCCCCCC
RIVAPESTDNAFQTSNPKIFAGGDAVRGSDLVVTAIAEGRKAADGIMSYLEV
CEECCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]