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The map label for this gene is ilvB [H]

Identifier: 157372190

GI number: 157372190

Start: 4384429

End: 4386072

Strand: Reverse

Name: ilvB [H]

Synonym: Spro_3956

Alternate gene names: 157372190

Gene position: 4386072-4384429 (Counterclockwise)

Preceding gene: 157372191

Following gene: 157372188

Centisome position: 80.5

GC content: 61.5

Gene sequence:

>1644_bases
ATGATGAGCGAGAAAATAACGGTTGGCGAGGCGATAGCCCGGACTCTGGAACAGTACGAGGTGTCGGCGATGTACGGCAT
CATTTCGATTCATAATCTGCCGATTGCCGATGCGGTGGGGCAGCGCGGCAACATTCGCTTTGTGCCGGCACGCGGTGAGG
CGGGGGCCGTGACCATGGCCGATGCCCACGGCCGTTTCTCCGGTCTGGGCGTGGCCTTGACCAGTACCGGTGCCGGTGCC
GGCAATGCGGTCGGTGCGATGATCGAGGCGATGAACGCCAATACGCCGTTGCTGCACATTACCGGTCAGGTGGAAAAAGC
CTGGCTGGATGCCGACGCCGGGTTCATTCATGAAACCCGCGATCAATTGGGTTTCCTGCGCGCCTGCTCCAAACGCGCGT
ATCGGGTTAACTCTGCGGAACAGGCGGTGGCAGTGATCCAACGGGCCATTCTCGACGCGCAGACGGTGCCTTGCGGCCCG
GTGGCGGTAGAGATCCCGATCGATATTCAAAGCAGCCTGGTTTCCAGCGCGGTGCTGAGCCAGCCGGTGATGCCTGCGCC
ACTGCCGCCAGCCAGTGACGACGTGGTTGAACGGTTGTATCAGCGGCTGAAACAGGCCAAACGCCCACTGTTATGGCTGG
GCGGCGGTGCGCTGGGCTGCGCTGAGGCGGTGCGTAAACTGGCGGATGCCGGTGTGGCAGTGATCTCCAGCACTCACGGG
CGCGGCATTCTGCCTGATAGCCACCCGCGCAGCCTGCGTGCGTTCCATAATTCGCCGAGCATTGAGGCGATCCTGACGCA
GTGCGATCTGACGCTGGTGGCCGGTTCACGCCTGCGCAGCAATGAAACACGTACCTGGACGCTGCCGCTGCCGCGCCCAT
TGGTACAGATTGATATTGATCCGGCGGCGGCCAATCGCAACTATTTGGCCGACGAACAAATCAACGGCGACTGTGGTGCC
TTGCTTAAAGCACTGGCTGCCCGAGTTAGCTCAGGTGAAAAGGTCAATGCCGAGTGGGATAGCGAAATCGCCCACGCGGT
GCAGCAGGCGGAAAGCGCGCTGCGCCAACAGTCGGGGGAATATGCCAAATTAAACGACGCCATTGCCGCCGCGCTGCCGC
AGGATGGGCTGCTGGTGCGTGATATCACCGTCTCCGGCAGCGTTTGGGGCAGCCGTTTGTTCCGCGCGATTTCGCCGCTG
TGTAACATTCACTCGCTGGCCGGGGCGATTGGCATGGGCTTGCCGATGGCGATTGGTACCGCGATCGCTAACCCGCAGCG
CAAGGTGGTCGGGCTGGTGGGCGACGGCGGGTTGGCGTTAGGCCTGGGAGAGCTGGCGACCATGGCGCAGGAGCAGGCCA
ATATCACTTTGCTGATTATGAACGACGGCGGTTACGGTGTGATGCGCGGCATTCAGGATAAATACTTTGCCGGTCGTCAG
TACTATAACGAACTGCACACTCCGGCCTTTACGCTGGTGGCCGAAGCTATGGGGCTGAAAGCCTGGAAGGTCGACAGCGC
GGCGCAGTTCAGCGGCGTCTTGGCAGAGGCGATTAACTATCCGGGGCCGTCGGTGGTGGAGGTGGACATGAACAGCATTG
GGCCGTTGACCTTCGCCGGACCACCGCAGAAAACCCTGTACTGA

Upstream 100 bases:

>100_bases
CAAAAAAGCCTGTATCTGGCGCTTAGCCATCAGGTGAATCGCTGGAGCGATTAGTTTTGAGCGACGGTGCATACCCTACA
ACGAACATATAAGAAGGCTG

Downstream 100 bases:

>100_bases
GTCGTGACAGATTGAACGACACCTTAATCAGTAAGGTGTCGTTCAAATGAAGTTAATTTTTATTAAATATTCCCAGGGCG
TCGACTATCAGTGAAATGGA

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MMSEKITVGEAIARTLEQYEVSAMYGIISIHNLPIADAVGQRGNIRFVPARGEAGAVTMADAHGRFSGLGVALTSTGAGA
GNAVGAMIEAMNANTPLLHITGQVEKAWLDADAGFIHETRDQLGFLRACSKRAYRVNSAEQAVAVIQRAILDAQTVPCGP
VAVEIPIDIQSSLVSSAVLSQPVMPAPLPPASDDVVERLYQRLKQAKRPLLWLGGGALGCAEAVRKLADAGVAVISSTHG
RGILPDSHPRSLRAFHNSPSIEAILTQCDLTLVAGSRLRSNETRTWTLPLPRPLVQIDIDPAAANRNYLADEQINGDCGA
LLKALAARVSSGEKVNAEWDSEIAHAVQQAESALRQQSGEYAKLNDAIAAALPQDGLLVRDITVSGSVWGSRLFRAISPL
CNIHSLAGAIGMGLPMAIGTAIANPQRKVVGLVGDGGLALGLGELATMAQEQANITLLIMNDGGYGVMRGIQDKYFAGRQ
YYNELHTPAFTLVAEAMGLKAWKVDSAAQFSGVLAEAINYPGPSVVEVDMNSIGPLTFAGPPQKTLY

Sequences:

>Translated_547_residues
MMSEKITVGEAIARTLEQYEVSAMYGIISIHNLPIADAVGQRGNIRFVPARGEAGAVTMADAHGRFSGLGVALTSTGAGA
GNAVGAMIEAMNANTPLLHITGQVEKAWLDADAGFIHETRDQLGFLRACSKRAYRVNSAEQAVAVIQRAILDAQTVPCGP
VAVEIPIDIQSSLVSSAVLSQPVMPAPLPPASDDVVERLYQRLKQAKRPLLWLGGGALGCAEAVRKLADAGVAVISSTHG
RGILPDSHPRSLRAFHNSPSIEAILTQCDLTLVAGSRLRSNETRTWTLPLPRPLVQIDIDPAAANRNYLADEQINGDCGA
LLKALAARVSSGEKVNAEWDSEIAHAVQQAESALRQQSGEYAKLNDAIAAALPQDGLLVRDITVSGSVWGSRLFRAISPL
CNIHSLAGAIGMGLPMAIGTAIANPQRKVVGLVGDGGLALGLGELATMAQEQANITLLIMNDGGYGVMRGIQDKYFAGRQ
YYNELHTPAFTLVAEAMGLKAWKVDSAAQFSGVLAEAINYPGPSVVEVDMNSIGPLTFAGPPQKTLY
>Mature_547_residues
MMSEKITVGEAIARTLEQYEVSAMYGIISIHNLPIADAVGQRGNIRFVPARGEAGAVTMADAHGRFSGLGVALTSTGAGA
GNAVGAMIEAMNANTPLLHITGQVEKAWLDADAGFIHETRDQLGFLRACSKRAYRVNSAEQAVAVIQRAILDAQTVPCGP
VAVEIPIDIQSSLVSSAVLSQPVMPAPLPPASDDVVERLYQRLKQAKRPLLWLGGGALGCAEAVRKLADAGVAVISSTHG
RGILPDSHPRSLRAFHNSPSIEAILTQCDLTLVAGSRLRSNETRTWTLPLPRPLVQIDIDPAAANRNYLADEQINGDCGA
LLKALAARVSSGEKVNAEWDSEIAHAVQQAESALRQQSGEYAKLNDAIAAALPQDGLLVRDITVSGSVWGSRLFRAISPL
CNIHSLAGAIGMGLPMAIGTAIANPQRKVVGLVGDGGLALGLGELATMAQEQANITLLIMNDGGYGVMRGIQDKYFAGRQ
YYNELHTPAFTLVAEAMGLKAWKVDSAAQFSGVLAEAINYPGPSVVEVDMNSIGPLTFAGPPQKTLY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=511, Percent_Identity=27.0058708414873, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI93004078, Length=486, Percent_Identity=24.2798353909465, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1790104, Length=541, Percent_Identity=28.2809611829945, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081685, Length=541, Percent_Identity=28.0961182994455, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=25.4867256637168, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1787096, Length=563, Percent_Identity=23.4458259325044, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=22.9802513464991, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17542570, Length=512, Percent_Identity=25.9765625, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=513, Percent_Identity=23.3918128654971, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17531301, Length=513, Percent_Identity=23.3918128654971, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=24.822695035461, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6320816, Length=561, Percent_Identity=22.9946524064171, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=517, Percent_Identity=22.4371373307544, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=517, Percent_Identity=21.4700193423598, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922626, Length=488, Percent_Identity=24.3852459016393, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57642; Mature: 57642

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSEKITVGEAIARTLEQYEVSAMYGIISIHNLPIADAVGQRGNIRFVPARGEAGAVTMA
CCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCCCCEEEEECCCCCCEEEEE
DAHGRFSGLGVALTSTGAGAGNAVGAMIEAMNANTPLLHITGQVEKAWLDADAGFIHETR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCHHHHHH
DQLGFLRACSKRAYRVNSAEQAVAVIQRAILDAQTVPCGPVAVEIPIDIQSSLVSSAVLS
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHC
QPVMPAPLPPASDDVVERLYQRLKQAKRPLLWLGGGALGCAEAVRKLADAGVAVISSTHG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCEEEEECCCC
RGILPDSHPRSLRAFHNSPSIEAILTQCDLTLVAGSRLRSNETRTWTLPLPRPLVQIDID
CCCCCCCCCHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCEEEEECC
PAAANRNYLADEQINGDCGALLKALAARVSSGEKVNAEWDSEIAHAVQQAESALRQQSGE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCC
YAKLNDAIAAALPQDGLLVRDITVSGSVWGSRLFRAISPLCNIHSLAGAIGMGLPMAIGT
CCHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AIANPQRKVVGLVGDGGLALGLGELATMAQEQANITLLIMNDGGYGVMRGIQDKYFAGRQ
HHCCCCEEEEEEECCCCCEECHHHHHHHHHHHCCEEEEEECCCCCHHHHCCHHHHHHHHH
YYNELHTPAFTLVAEAMGLKAWKVDSAAQFSGVLAEAINYPGPSVVEVDMNSIGPLTFAG
HHHHHCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECC
PPQKTLY
CCCCCCC
>Mature Secondary Structure
MMSEKITVGEAIARTLEQYEVSAMYGIISIHNLPIADAVGQRGNIRFVPARGEAGAVTMA
CCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCCCCEEEEECCCCCCEEEEE
DAHGRFSGLGVALTSTGAGAGNAVGAMIEAMNANTPLLHITGQVEKAWLDADAGFIHETR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCHHHHHH
DQLGFLRACSKRAYRVNSAEQAVAVIQRAILDAQTVPCGPVAVEIPIDIQSSLVSSAVLS
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHC
QPVMPAPLPPASDDVVERLYQRLKQAKRPLLWLGGGALGCAEAVRKLADAGVAVISSTHG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCEEEEECCCC
RGILPDSHPRSLRAFHNSPSIEAILTQCDLTLVAGSRLRSNETRTWTLPLPRPLVQIDID
CCCCCCCCCHHHHHHCCCCCHHHHHHHCCEEEEECCCCCCCCCEEEECCCCCCEEEEECC
PAAANRNYLADEQINGDCGALLKALAARVSSGEKVNAEWDSEIAHAVQQAESALRQQSGE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHCCCC
YAKLNDAIAAALPQDGLLVRDITVSGSVWGSRLFRAISPLCNIHSLAGAIGMGLPMAIGT
CCHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AIANPQRKVVGLVGDGGLALGLGELATMAQEQANITLLIMNDGGYGVMRGIQDKYFAGRQ
HHCCCCEEEEEEECCCCCEECHHHHHHHHHHHCCEEEEEECCCCCHHHHCCHHHHHHHHH
YYNELHTPAFTLVAEAMGLKAWKVDSAAQFSGVLAEAINYPGPSVVEVDMNSIGPLTFAG
HHHHHCCHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECC
PPQKTLY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]