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The map label for this gene is gcvT

Identifier: 157372150

GI number: 157372150

Start: 4338728

End: 4339825

Strand: Reverse

Name: gcvT

Synonym: Spro_3916

Alternate gene names: 157372150

Gene position: 4339825-4338728 (Counterclockwise)

Preceding gene: 157372151

Following gene: 157372149

Centisome position: 79.65

GC content: 58.38

Gene sequence:

>1098_bases
ATGGCAAAGCAAACCCCACTGTACGACCAGCACGTGGCGTGCGGTGCGCGCATGGTAGATTTTCATGGCTGGATGATGCC
GCTGCATTACGGCTCCCAGATCGATGAGCACCACGCCGTGCGTCAGGATGCCGGTATGTTCGACGTCTCCCACATGACCA
TTGTGGATCTGCGCGGTGCCCGCACCCGCGAGTTCCTGCGTTACCTGCTGGCGAATGACGTCGCCAAGCTCACTCAACCT
GGCAAAGCGTTATACACCGGTATGCTGAATGCGTCCGGCGGTGTGATTGACGACCTGATTGTTTACTTCCTTACTGAAGA
CTATTTCCGCCTGGTGGTGAACTCCGCCACACGCGAAAAAGACCTGGCCTGGATCGAAGAGCACGCAGCCCCTTACGGCG
TAGCCCTGACGGTGCGTGACGATCTGGCGCTGGTTGCGGTGCAGGGCCCACAGGCCAAAGAGCGCGCCGCCACCTTATTT
ACCCCGGAACAAAAAAGCGCGGTTGAAGGCATGAAGCCATTCTTCGGCGTACAGGCCGGGGATCTGTTTATTGCCACCAC
CGGTTATACCGGCGAAGCGGGCTATGAAATCGCCCTGCCGAAAGAGCAGGTGGTCGATTTCTGGCAAAAACTGCTGGCAG
CAGGTGTGAAGCCTGCCGGCCTGGGCGCACGCGACACGCTGCGTCTGGAGGCAGGCATGAACCTGTACGGGCAAGAAATG
GACGAAGGCGTTTCGCCGCTGGCCGCCAACATGGGCTGGACCATTGCCTGGCTGCCGGAAGACCGTCAGTTTATTGGCCG
TGAAGCGCTGGAAAAACAGCGTGAAATGGGTACCGAGCAACTGGTCGGCTTGATCATGACGGAAAAAGGCGTATTACGTA
ATGAGCTGCCGGTGCGATTCACCGATGAAGCAGGTCAGACGCATGAAGGGATTATCACCAGCGGTTCATTCTCACCGACC
CTGGGCTTCAGTATTGCCCTGGCCCGCGTGCCCGCTGGTATCGGCGAGCAGGCCATCGTGCAGATCCGCAACCGTGAAAT
GCCGGTCAAGGTCACCAAACCTGGATTCGTGCGTGCCGGTAAGCCACTGACTAATTAA

Upstream 100 bases:

>100_bases
TTGGGCAGTCAGTGCGCCACGCAATCCGGCCGCGAGCCATTATCTGATGTATTGCGTAGCAGCACCCTATCTTCAGCTTC
TGAGTGAAATGAGGGAAAAG

Downstream 100 bases:

>100_bases
TTTTTTGTTTATTGATTCTTTATTTCTTCGAAGGAGTAGCTGGCGATGAGCAATGTGCCAACAGAATTAAAGTATGCATC
TTCCCATGAGTGGGTTCGTT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MAKQTPLYDQHVACGARMVDFHGWMMPLHYGSQIDEHHAVRQDAGMFDVSHMTIVDLRGARTREFLRYLLANDVAKLTQP
GKALYTGMLNASGGVIDDLIVYFLTEDYFRLVVNSATREKDLAWIEEHAAPYGVALTVRDDLALVAVQGPQAKERAATLF
TPEQKSAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPKEQVVDFWQKLLAAGVKPAGLGARDTLRLEAGMNLYGQEM
DEGVSPLAANMGWTIAWLPEDRQFIGREALEKQREMGTEQLVGLIMTEKGVLRNELPVRFTDEAGQTHEGIITSGSFSPT
LGFSIALARVPAGIGEQAIVQIRNREMPVKVTKPGFVRAGKPLTN

Sequences:

>Translated_365_residues
MAKQTPLYDQHVACGARMVDFHGWMMPLHYGSQIDEHHAVRQDAGMFDVSHMTIVDLRGARTREFLRYLLANDVAKLTQP
GKALYTGMLNASGGVIDDLIVYFLTEDYFRLVVNSATREKDLAWIEEHAAPYGVALTVRDDLALVAVQGPQAKERAATLF
TPEQKSAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPKEQVVDFWQKLLAAGVKPAGLGARDTLRLEAGMNLYGQEM
DEGVSPLAANMGWTIAWLPEDRQFIGREALEKQREMGTEQLVGLIMTEKGVLRNELPVRFTDEAGQTHEGIITSGSFSPT
LGFSIALARVPAGIGEQAIVQIRNREMPVKVTKPGFVRAGKPLTN
>Mature_364_residues
AKQTPLYDQHVACGARMVDFHGWMMPLHYGSQIDEHHAVRQDAGMFDVSHMTIVDLRGARTREFLRYLLANDVAKLTQPG
KALYTGMLNASGGVIDDLIVYFLTEDYFRLVVNSATREKDLAWIEEHAAPYGVALTVRDDLALVAVQGPQAKERAATLFT
PEQKSAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPKEQVVDFWQKLLAAGVKPAGLGARDTLRLEAGMNLYGQEMD
EGVSPLAANMGWTIAWLPEDRQFIGREALEKQREMGTEQLVGLIMTEKGVLRNELPVRFTDEAGQTHEGIITSGSFSPTL
GFSIALARVPAGIGEQAIVQIRNREMPVKVTKPGFVRAGKPLTN

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=377, Percent_Identity=32.3607427055703, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI257796258, Length=349, Percent_Identity=32.9512893982808, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI257796254, Length=372, Percent_Identity=29.3010752688172, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI257796256, Length=320, Percent_Identity=30, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI24797151, Length=354, Percent_Identity=27.4011299435028, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI197927446, Length=318, Percent_Identity=27.3584905660377, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI21361378, Length=318, Percent_Identity=27.3584905660377, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1789272, Length=363, Percent_Identity=82.9201101928375, Blast_Score=638, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17560118, Length=379, Percent_Identity=34.5646437994723, Blast_Score=170, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI32563613, Length=346, Percent_Identity=24.8554913294798, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71994052, Length=340, Percent_Identity=22.3529411764706, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994045, Length=340, Percent_Identity=22.3529411764706, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320222, Length=376, Percent_Identity=35.1063829787234, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=33.6870026525199, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI28571104, Length=275, Percent_Identity=28.7272727272727, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI20130091, Length=362, Percent_Identity=25.1381215469613, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_SERP5 (A8GIS1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480139.1
- ProteinModelPortal:   A8GIS1
- SMR:   A8GIS1
- STRING:   A8GIS1
- GeneID:   5604356
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3916
- eggNOG:   COG0404
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- BioCyc:   SPRO399741:SPRO_3916-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40082; Mature: 39951

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQTPLYDQHVACGARMVDFHGWMMPLHYGSQIDEHHAVRQDAGMFDVSHMTIVDLRGA
CCCCCCCCHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHHCCCCEEECCEEEEECCCC
RTREFLRYLLANDVAKLTQPGKALYTGMLNASGGVIDDLIVYFLTEDYFRLVVNSATREK
HHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
DLAWIEEHAAPYGVALTVRDDLALVAVQGPQAKERAATLFTPEQKSAVEGMKPFFGVQAG
HHHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHHEEECCCHHHHHHCCCHHHCCCCC
DLFIATTGYTGEAGYEIALPKEQVVDFWQKLLAAGVKPAGLGARDTLRLEAGMNLYGQEM
CEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHH
DEGVSPLAANMGWTIAWLPEDRQFIGREALEKQREMGTEQLVGLIMTEKGVLRNELPVRF
HCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCEEE
TDEAGQTHEGIITSGSFSPTLGFSIALARVPAGIGEQAIVQIRNREMPVKVTKPGFVRAG
CCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCEECC
KPLTN
CCCCC
>Mature Secondary Structure 
AKQTPLYDQHVACGARMVDFHGWMMPLHYGSQIDEHHAVRQDAGMFDVSHMTIVDLRGA
CCCCCCCHHHHHCCCEEEECCCEEEEECCCCCCHHHHHHHHCCCCEEECCEEEEECCCC
RTREFLRYLLANDVAKLTQPGKALYTGMLNASGGVIDDLIVYFLTEDYFRLVVNSATREK
HHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
DLAWIEEHAAPYGVALTVRDDLALVAVQGPQAKERAATLFTPEQKSAVEGMKPFFGVQAG
HHHHHHHHCCCCEEEEEEECCEEEEEECCCCCHHHHHEEECCCHHHHHHCCCHHHCCCCC
DLFIATTGYTGEAGYEIALPKEQVVDFWQKLLAAGVKPAGLGARDTLRLEAGMNLYGQEM
CEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHH
DEGVSPLAANMGWTIAWLPEDRQFIGREALEKQREMGTEQLVGLIMTEKGVLRNELPVRF
HCCCCCHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCCCEEE
TDEAGQTHEGIITSGSFSPTLGFSIALARVPAGIGEQAIVQIRNREMPVKVTKPGFVRAG
CCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCEECC
KPLTN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA