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The map label for this gene is gcvH

Identifier: 157372149

GI number: 157372149

Start: 4338296

End: 4338682

Strand: Reverse

Name: gcvH

Synonym: Spro_3915

Alternate gene names: 157372149

Gene position: 4338682-4338296 (Counterclockwise)

Preceding gene: 157372150

Following gene: 157372148

Centisome position: 79.63

GC content: 56.07

Gene sequence:

>387_bases
ATGAGCAATGTGCCAACAGAATTAAAGTATGCATCTTCCCATGAGTGGGTTCGTTCAGAAGGTAACGGCGTTTACACCGT
GGGCATCACCGAACACGCGCAGGAACTGCTGGGCGATATGGTATTTGTCGATCTGCCGGAAGTAGGCCGCACCGTTGCCG
CCGGTGAAGACTGCGCCGTGGCGGAGTCCGTCAAGGCGGCCTCTGACATTTACGCGCCAATCAGCGGCGAAATCGTTGCG
GTAAACGGCGAACTGGAAAGTTCTCCGGAGCTGGTCAACAGCGGGCCATACGCTGAAGGCTTCCTGTTCCAGATTAAAGC
CTCAGACGAAGGCGAACTGGCGAAGCTGCTGGACGCCACGGCTTATCAGGCTTCTATCGACGAGTAA

Upstream 100 bases:

>100_bases
GGTCAAGGTCACCAAACCTGGATTCGTGCGTGCCGGTAAGCCACTGACTAATTAATTTTTTGTTTATTGATTCTTTATTT
CTTCGAAGGAGTAGCTGGCG

Downstream 100 bases:

>100_bases
CCGTTGCAACGCCCCAGGCCTATTGGTCGGGGCGTTTTAGTTACAGAACAGGCTATGTCCCGTACCCCGTAAGCATTCAG
GAATTTGTAGCAATGACTCA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSNVPTELKYASSHEWVRSEGNGVYTVGITEHAQELLGDMVFVDLPEVGRTVAAGEDCAVAESVKAASDIYAPISGEIVA
VNGELESSPELVNSGPYAEGFLFQIKASDEGELAKLLDATAYQASIDE

Sequences:

>Translated_128_residues
MSNVPTELKYASSHEWVRSEGNGVYTVGITEHAQELLGDMVFVDLPEVGRTVAAGEDCAVAESVKAASDIYAPISGEIVA
VNGELESSPELVNSGPYAEGFLFQIKASDEGELAKLLDATAYQASIDE
>Mature_127_residues
SNVPTELKYASSHEWVRSEGNGVYTVGITEHAQELLGDMVFVDLPEVGRTVAAGEDCAVAESVKAASDIYAPISGEIVAV
NGELESSPELVNSGPYAEGFLFQIKASDEGELAKLLDATAYQASIDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=43.6974789915966, Blast_Score=103, Evalue=5e-23,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=42.8571428571429, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=76.5625, Blast_Score=203, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=48.6725663716814, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=114, Percent_Identity=42.9824561403509, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=102, Percent_Identity=41.1764705882353, Blast_Score=91, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=42.3728813559322, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_SERP5 (A8GIS0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001480138.1
- ProteinModelPortal:   A8GIS0
- SMR:   A8GIS0
- STRING:   A8GIS0
- GeneID:   5604355
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3915
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   SASEYEQ
- BioCyc:   SPRO399741:SPRO_3915-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13539; Mature: 13408

Theoretical pI: Translated: 3.80; Mature: 3.80

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPTELKYASSHEWVRSEGNGVYTVGITEHAQELLGDMVFVDLPEVGRTVAAGEDCAV
CCCCCCCEEECCCCCHHHCCCCEEEEEECHHHHHHHHHCEEEEECCCCCCEECCCCCCHH
AESVKAASDIYAPISGEIVAVNGELESSPELVNSGPYAEGFLFQIKASDEGELAKLLDAT
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
AYQASIDE
HHHCCCCC
>Mature Secondary Structure 
SNVPTELKYASSHEWVRSEGNGVYTVGITEHAQELLGDMVFVDLPEVGRTVAAGEDCAV
CCCCCCEEECCCCCHHHCCCCEEEEEECHHHHHHHHHCEEEEECCCCCCEECCCCCCHH
AESVKAASDIYAPISGEIVAVNGELESSPELVNSGPYAEGFLFQIKASDEGELAKLLDAT
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
AYQASIDE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA