Click here to switch to the map view.

The map label for this gene is dmlR [H]

Identifier: 157372099

GI number: 157372099

Start: 4276937

End: 4277851

Strand: Reverse

Name: dmlR [H]

Synonym: Spro_3864

Alternate gene names: 157372099

Gene position: 4277851-4276937 (Counterclockwise)

Preceding gene: 157372110

Following gene: 157372098

Centisome position: 78.51

GC content: 49.51

Gene sequence:

>915_bases
ATGCAAAATCGGCTGGAGGCATTGCAAATTTTTTGTGCCGCTGCGGAAGAAAGCAGTTTTAAACAGGTGGCGATAAGGCT
GGCAATCTCACCGCAGAAGGTGACCCGTGCGATAAAAGAACTGGAGCGGTTACGAGGGGAGCAACTGTTCCACCGTAACA
CCCGGCAAATAAAAATCACCCAATTTGGCGCTGATTTTGCGGAGCAGGCGAAACAGGTCATTAGCCAGTTTGAACAGCTA
TTTCCTGATAAGCATAAAATGCGAGAAGACATTCGTGGCATTGTGCGTATCACGGCGCCCAATACCTTTGGCCGTGAGGT
GGTCATGCCTGCCTTAACCGAGCTGGCCGTTCTTTATCCGGCGATTACACTGGAATTTCATCTCGCCAATACTCACAGCA
ATCTAGTGGATGAGGAAATTGATATTGGCGTACGCATTGGCTTCTTGCATGACCAAACCTGCGTCGTCCGGCAGCCGAAA
ATGATGCCTTTTTATTTGGTGGCGGCGCCGTCATTGCTTGCACGTACGGGCTTGCCGAAGAGCATTGAGGAATTATCGTC
TTTACCGATCATTGCGTTGATAAACCGTAAAACCGGAAGATATTGGCCGTGGGAATTCAGTAACAAACGTCGTTTTCATC
CAGAACAGGCCGCTTTTATCACTGACGATCCGGATATCGAACGCAATGCAGCCCTGGCAGGAATAGGCTTTGGTCATATG
GCGGGCTACCTGGTTGAAAAATATCTGGCCAGTGGGCAGTTGATCTCGGTATTGCCAAATGATGCACCGGAACCTTGGCC
GCTGAATTTATATCGGCCATATCAGGAGCCGGTACCGGCCAGAATTAGACTGGTTTTTGACTATCTCTATGAGAAGTTAA
GCCAGCCTGCGTATGGACTCGATGACGCTGGCTAG

Upstream 100 bases:

>100_bases
TTGCAATCGAGGCTCATATACTGACGTTTTAACGCTGCCGGTAAATGGAAATGTTATACTTTCAAAATTCCAAATTATGG
AATAGTGATGAGGTGACAGG

Downstream 100 bases:

>100_bases
CGTGAGCTGGGCCAGTTTGGTATTAGATTGAAGGCAGCCGTAATCTCCGGTGCATTCCAGAATATTATCTGCCTGCAAAT
ACCGTGTAATTTACGCTAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MQNRLEALQIFCAAAEESSFKQVAIRLAISPQKVTRAIKELERLRGEQLFHRNTRQIKITQFGADFAEQAKQVISQFEQL
FPDKHKMREDIRGIVRITAPNTFGREVVMPALTELAVLYPAITLEFHLANTHSNLVDEEIDIGVRIGFLHDQTCVVRQPK
MMPFYLVAAPSLLARTGLPKSIEELSSLPIIALINRKTGRYWPWEFSNKRRFHPEQAAFITDDPDIERNAALAGIGFGHM
AGYLVEKYLASGQLISVLPNDAPEPWPLNLYRPYQEPVPARIRLVFDYLYEKLSQPAYGLDDAG

Sequences:

>Translated_304_residues
MQNRLEALQIFCAAAEESSFKQVAIRLAISPQKVTRAIKELERLRGEQLFHRNTRQIKITQFGADFAEQAKQVISQFEQL
FPDKHKMREDIRGIVRITAPNTFGREVVMPALTELAVLYPAITLEFHLANTHSNLVDEEIDIGVRIGFLHDQTCVVRQPK
MMPFYLVAAPSLLARTGLPKSIEELSSLPIIALINRKTGRYWPWEFSNKRRFHPEQAAFITDDPDIERNAALAGIGFGHM
AGYLVEKYLASGQLISVLPNDAPEPWPLNLYRPYQEPVPARIRLVFDYLYEKLSQPAYGLDDAG
>Mature_304_residues
MQNRLEALQIFCAAAEESSFKQVAIRLAISPQKVTRAIKELERLRGEQLFHRNTRQIKITQFGADFAEQAKQVISQFEQL
FPDKHKMREDIRGIVRITAPNTFGREVVMPALTELAVLYPAITLEFHLANTHSNLVDEEIDIGVRIGFLHDQTCVVRQPK
MMPFYLVAAPSLLARTGLPKSIEELSSLPIIALINRKTGRYWPWEFSNKRRFHPEQAAFITDDPDIERNAALAGIGFGHM
AGYLVEKYLASGQLISVLPNDAPEPWPLNLYRPYQEPVPARIRLVFDYLYEKLSQPAYGLDDAG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=305, Percent_Identity=28.8524590163934, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=24.6666666666667, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=27.3356401384083, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=26.6666666666667, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=23.6486486486486, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789639, Length=305, Percent_Identity=23.6065573770492, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=23.1833910034602, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788748, Length=127, Percent_Identity=27.5590551181102, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1786448, Length=265, Percent_Identity=23.0188679245283, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693105, Length=156, Percent_Identity=27.5641025641026, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34587; Mature: 34587

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNRLEALQIFCAAAEESSFKQVAIRLAISPQKVTRAIKELERLRGEQLFHRNTRQIKIT
CCHHHHHHHHHHHHHCCCHHHEEHEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
QFGADFAEQAKQVISQFEQLFPDKHKMREDIRGIVRITAPNTFGREVVMPALTELAVLYP
ECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHH
AITLEFHLANTHSNLVDEEIDIGVRIGFLHDQTCVVRQPKMMPFYLVAAPSLLARTGLPK
HEEEEEEEECCCCHHCCHHCCCEEEEEEEECCEEEEECCCCCCEEEECCHHHHHHCCCCH
SIEELSSLPIIALINRKTGRYWPWEFSNKRRFHPEQAAFITDDPDIERNAALAGIGFGHM
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCHHHH
AGYLVEKYLASGQLISVLPNDAPEPWPLNLYRPYQEPVPARIRLVFDYLYEKLSQPAYGL
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
DDAG
CCCC
>Mature Secondary Structure
MQNRLEALQIFCAAAEESSFKQVAIRLAISPQKVTRAIKELERLRGEQLFHRNTRQIKIT
CCHHHHHHHHHHHHHCCCHHHEEHEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
QFGADFAEQAKQVISQFEQLFPDKHKMREDIRGIVRITAPNTFGREVVMPALTELAVLYP
ECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHH
AITLEFHLANTHSNLVDEEIDIGVRIGFLHDQTCVVRQPKMMPFYLVAAPSLLARTGLPK
HEEEEEEEECCCCHHCCHHCCCEEEEEEEECCEEEEECCCCCCEEEECCHHHHHHCCCCH
SIEELSSLPIIALINRKTGRYWPWEFSNKRRFHPEQAAFITDDPDIERNAALAGIGFGHM
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCHHHH
AGYLVEKYLASGQLISVLPNDAPEPWPLNLYRPYQEPVPARIRLVFDYLYEKLSQPAYGL
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
DDAG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]