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The map label for this gene is dcuB [H]

Identifier: 157372098

GI number: 157372098

Start: 4275274

End: 4276614

Strand: Reverse

Name: dcuB [H]

Synonym: Spro_3863

Alternate gene names: 157372098

Gene position: 4276614-4275274 (Counterclockwise)

Preceding gene: 157372099

Following gene: 157372093

Centisome position: 78.49

GC content: 49.37

Gene sequence:

>1341_bases
ATGTTATTTACCATTCAACTTATTGTCATACTGATCTGTCTATTTTATGGAGCCAGAAAAGGTGGGATTGCACTGGGGCT
GCTCGGCGGTATTGGATTAATCATCCTGGTTTTCGTTTTTCATTTACCGCCCGGAAAACCGCCGGTTGACGTTATGCTGG
TTATCATCGCCGTGGTGGCCGCATCGGCGACGTTGCAGGCATCCGGTGGGCTTGACGTGATGCTGCAGATTGCCGAGCGT
ATGCTGCGCCGCAATCCTAAATATGTCTCCATCGTCGCTCCCTTTGTGACCTGCATCCTGACCATTCTTTGCGGTACCGG
GCATGTGGTATACACCATTTTGCCGATTATTTATGATGTGGCAATCAAGAATAACATTCGCCCGGAAAGGCCGATGGCAG
CCAGTTCTATCGGGGCACAGATGGGCATTATAGCCAGCCCGGTATCCGTCGCTGTGGTGTCGCTGGTCGCCATGTTGGGT
AATTTTACCTTCAATGGCAAGCACCTCGAGTTTCTCGATCTGCTGGCCATTACTATTCCCTCGACCCTGTGCGGCATTCT
GGCTATTGGTATATTCAGTTGGTTCCGGGGTAAGGATCTGGATAAGGATGAAAAGTTCCAAAACTTCATTTCAGACCCAG
AAAACTATAAATACGTTTATGGCGAAACGGCAACATTACTGGATAAGAAATTGCCCACCAGCAACTGGGTGGCTATGTGG
ATTTTTCTGGCTACCATAGCTATTGTTGCGCTATTGGGCGCTTTTTCTGAACTACGGCCATTAATTAATGGAAAACCACT
TTCCATGGTATTGGTCATCCAGATGTTTATGCTGTGTGCAGGTGCAGTAATTATTATCGCGACCAAAACGAACCCGGCCA
ATATCTCAAAAAACGAGGTTTTCCGTTCAGGCATGATTGCCATAGTGGCGGTTTACGGTATTGCCTGGATGGCCGAAACC
ATGTTTGGTGCGCATTTGGAAGAGATAAAGTCCGTACTGGGAACCCTGGTTAAAGAGTATCCCTGGGCCTACGCTATCAT
TTTGCTGTTGGTTTCCAAGTTTGTTAACTCCCAGGCCGCTGCACTTGCAGCCATCGTCCCTGTCGCATTGGCTATCGGTG
TTGATCCGGCCTATATCGTAGCTTCTGCTCCCGCCTGCTATGGCTACTATATTTTACCCACCTACCCAAGCGATTTGGCG
GCTATTCAGTTCGATCGCTCCGGGACAACCAACATCGGACGGTTCGTCATCAACCACAGTTTCATCATGGTGGGTTTGAT
CGGGGTTTCCGTATCCTGCGTGATGGGTTGGGTGCTGGCCGCGGCCTACGGGTTCTTGTAA

Upstream 100 bases:

>100_bases
TTTTTTAACTTAAAAAACCATCTGTTTGTGATCGCTATCCCTCAATGGAAATGAAAATAAGTTAACTATTAACCTTCTTA
ACAATAATAATGAGGTTAGC

Downstream 100 bases:

>100_bases
CGAGGGGGGCGTAGCGCTGGATAATACTGTTTGTCGATGAGTTACTATTTTTCTTAGATGTGAAATGCAAAAAACCCGCT
CAACGCGGGTTTTTTATCAC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLFTIQLIVILICLFYGARKGGIALGLLGGIGLIILVFVFHLPPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAER
MLRRNPKYVSIVAPFVTCILTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NFTFNGKHLEFLDLLAITIPSTLCGILAIGIFSWFRGKDLDKDEKFQNFISDPENYKYVYGETATLLDKKLPTSNWVAMW
IFLATIAIVALLGAFSELRPLINGKPLSMVLVIQMFMLCAGAVIIIATKTNPANISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHLEEIKSVLGTLVKEYPWAYAIILLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTNIGRFVINHSFIMVGLIGVSVSCVMGWVLAAAYGFL

Sequences:

>Translated_446_residues
MLFTIQLIVILICLFYGARKGGIALGLLGGIGLIILVFVFHLPPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAER
MLRRNPKYVSIVAPFVTCILTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NFTFNGKHLEFLDLLAITIPSTLCGILAIGIFSWFRGKDLDKDEKFQNFISDPENYKYVYGETATLLDKKLPTSNWVAMW
IFLATIAIVALLGAFSELRPLINGKPLSMVLVIQMFMLCAGAVIIIATKTNPANISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHLEEIKSVLGTLVKEYPWAYAIILLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTNIGRFVINHSFIMVGLIGVSVSCVMGWVLAAAYGFL
>Mature_446_residues
MLFTIQLIVILICLFYGARKGGIALGLLGGIGLIILVFVFHLPPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAER
MLRRNPKYVSIVAPFVTCILTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NFTFNGKHLEFLDLLAITIPSTLCGILAIGIFSWFRGKDLDKDEKFQNFISDPENYKYVYGETATLLDKKLPTSNWVAMW
IFLATIAIVALLGAFSELRPLINGKPLSMVLVIQMFMLCAGAVIIIATKTNPANISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHLEEIKSVLGTLVKEYPWAYAIILLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTNIGRFVINHSFIMVGLIGVSVSCVMGWVLAAAYGFL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=446, Percent_Identity=89.9103139013453, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1790580, Length=442, Percent_Identity=35.0678733031674, Blast_Score=255, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 47931; Mature: 47931

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTIQLIVILICLFYGARKGGIALGLLGGIGLIILVFVFHLPPGKPPVDVMLVIIAVVA
CEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ASATLQASGGLDVMLQIAERMLRRNPKYVSIVAPFVTCILTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNFTFNGKHLEFLDLLAITIP
HHHCCCCCCCCCHHHHHCHHHCHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
STLCGILAIGIFSWFRGKDLDKDEKFQNFISDPENYKYVYGETATLLDKKLPTSNWVAMW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCHHHHHH
IFLATIAIVALLGAFSELRPLINGKPLSMVLVIQMFMLCAGAVIIIATKTNPANISKNEV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHLEEIKSVLGTLVKEYPWAYAIILLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTNIGRFVINHS
HHHHHHHHHHHHCCCHHHHEECCCCHHCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHH
FIMVGLIGVSVSCVMGWVLAAAYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFTIQLIVILICLFYGARKGGIALGLLGGIGLIILVFVFHLPPGKPPVDVMLVIIAVVA
CEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ASATLQASGGLDVMLQIAERMLRRNPKYVSIVAPFVTCILTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNFTFNGKHLEFLDLLAITIP
HHHCCCCCCCCCHHHHHCHHHCHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
STLCGILAIGIFSWFRGKDLDKDEKFQNFISDPENYKYVYGETATLLDKKLPTSNWVAMW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCHHHHHH
IFLATIAIVALLGAFSELRPLINGKPLSMVLVIQMFMLCAGAVIIIATKTNPANISKNEV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHLEEIKSVLGTLVKEYPWAYAIILLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTNIGRFVINHS
HHHHHHHHHHHHCCCHHHHEECCCCHHCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHH
FIMVGLIGVSVSCVMGWVLAAAYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]