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The map label for this gene is gcvA [H]

Identifier: 157372040

GI number: 157372040

Start: 4205115

End: 4206032

Strand: Reverse

Name: gcvA [H]

Synonym: Spro_3805

Alternate gene names: 157372040

Gene position: 4206032-4205115 (Counterclockwise)

Preceding gene: 157372041

Following gene: 157372039

Centisome position: 77.19

GC content: 54.25

Gene sequence:

>918_bases
ATGTCTAAACGCTTACCACCTCTTAATGCACTGCGGGTTTTTGACGCGGCTGCCCGTCACCTGAGTTTTACCAAAGCGGC
AGAAGAACTGTTTGTCACCCAGGCCGCGGTGAGCCACCAGATCAAGTCGCTGGAGGACTTCCTCGGCCTGAAGCTGTTTC
GGCGCCGCAATCGCTCGCTGTTGCTGACGGAAGAAGGGCAAAGTTATTACCTGGATATCAAAGAAATTTTCACCTCAATC
AATGAGGCCACCCGCAAGCTGCAGGCCCGCAGCGCCAAGGGGGCATTAACCGTCAGTTTGCCCCCTAGTTTTGCTATTCA
GTGGCTGGTGCCGCGCCTGTCCGGCTTTAACTCAGCTTATCCGGGAATTGACGTGCGTATTCAGGCCGTGGACCGCGAAG
AAGACAAGCTGGCGGATGATGTCGACGTGGCTATTTTCTATGGACGCGGCAACTGGACCGGATTGCGAGCCGAACGTTTA
TACGCGGAATATCTGTTGCCGGTGTGCTCACCCAGCCTGCTGACCGGGGAACATGCGTTAAAAATGACGAGCGATCTGGC
TTATCACACGCTACTGCATGATACTTCACGCCGCGATTGGCTGGCCTATACCCGTCAGTTGGGGTTGCAGCACATTAATG
TGCAGCAGGGGCCTATTTTCAGCCATAGCGCGATGGTGGTTCAGGCGGCGGTGCATGGGCAGGGGATTGCGCTGGTGAAT
AACGTGATGGCGCAGACCGAGATTGAAGCCGGGCGTTTGGTCTGTCCGTTTAACGAGGTTTTAGTCAGTAAAAATGCTTT
TTATCTGGTATGTCATGACAGCCAGGCAGAACTGGGTAAAATAGCCGCCTTTCGTCAGTGGATCCTGGCTCGGGCAGCCA
GCGAACAAGAAAAGTTCCGCTTTCGCTACGAACAATGA

Upstream 100 bases:

>100_bases
GTAGCGCTTTGCTGTCTGGTGCCTATCGCACGGCAGATAACGCCGCCTGATTTTTTATGCCGAATCACTCGGATTATTGT
TACACAAAAGTAGCTAATAA

Downstream 100 bases:

>100_bases
GGCCGGTCGCCGCGATGGCGCAGCCGGTTTTCTTCAATAAAGGTAAATAACGATGAGCAGTCGTTCAATGCTGATTTTTG
CCGCTATCAGTGGCTTTGTA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKRLPPLNALRVFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFTSI
NEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNSAYPGIDVRIQAVDREEDKLADDVDVAIFYGRGNWTGLRAERL
YAEYLLPVCSPSLLTGEHALKMTSDLAYHTLLHDTSRRDWLAYTRQLGLQHINVQQGPIFSHSAMVVQAAVHGQGIALVN
NVMAQTEIEAGRLVCPFNEVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKFRFRYEQ

Sequences:

>Translated_305_residues
MSKRLPPLNALRVFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFTSI
NEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNSAYPGIDVRIQAVDREEDKLADDVDVAIFYGRGNWTGLRAERL
YAEYLLPVCSPSLLTGEHALKMTSDLAYHTLLHDTSRRDWLAYTRQLGLQHINVQQGPIFSHSAMVVQAAVHGQGIALVN
NVMAQTEIEAGRLVCPFNEVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKFRFRYEQ
>Mature_304_residues
SKRLPPLNALRVFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYYLDIKEIFTSIN
EATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNSAYPGIDVRIQAVDREEDKLADDVDVAIFYGRGNWTGLRAERLY
AEYLLPVCSPSLLTGEHALKMTSDLAYHTLLHDTSRRDWLAYTRQLGLQHINVQQGPIFSHSAMVVQAAVHGQGIALVNN
VMAQTEIEAGRLVCPFNEVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKFRFRYEQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=89.5081967213115, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=31.8339100346021, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=33.9160839160839, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=28.3333333333333, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=27.8169014084507, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=31.9066147859922, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=33.1210191082803, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=27.6, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=31.8518518518519, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=30.327868852459, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790783, Length=251, Percent_Identity=27.8884462151394, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=30.7692307692308, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34395; Mature: 34264

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLPPLNALRVFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYYLDIKEIFTSINEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNSAY
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
PGIDVRIQAVDREEDKLADDVDVAIFYGRGNWTGLRAERLYAEYLLPVCSPSLLTGEHAL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHH
KMTSDLAYHTLLHDTSRRDWLAYTRQLGLQHINVQQGPIFSHSAMVVQAAVHGQGIALVN
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEECCCCCCCCCCCHHHEEHHHCCCCHHHHH
NVMAQTEIEAGRLVCPFNEVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKFR
HHHHHHECCCCCEECCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHH
FRYEQ
CCCCC
>Mature Secondary Structure 
SKRLPPLNALRVFDAAARHLSFTKAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYYLDIKEIFTSINEATRKLQARSAKGALTVSLPPSFAIQWLVPRLSGFNSAY
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
PGIDVRIQAVDREEDKLADDVDVAIFYGRGNWTGLRAERLYAEYLLPVCSPSLLTGEHAL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHH
KMTSDLAYHTLLHDTSRRDWLAYTRQLGLQHINVQQGPIFSHSAMVVQAAVHGQGIALVN
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEECCCCCCCCCCCHHHEEHHHCCCCHHHHH
NVMAQTEIEAGRLVCPFNEVLVSKNAFYLVCHDSQAELGKIAAFRQWILARAASEQEKFR
HHHHHHECCCCCEECCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHH
FRYEQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]