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The map label for this gene is ypfH [H]

Identifier: 157371741

GI number: 157371741

Start: 3879100

End: 3879789

Strand: Reverse

Name: ypfH [H]

Synonym: Spro_3504

Alternate gene names: 157371741

Gene position: 3879789-3879100 (Counterclockwise)

Preceding gene: 157371742

Following gene: 157371736

Centisome position: 71.2

GC content: 57.83

Gene sequence:

>690_bases
ATGAAACATGAGCATTTTGTTGTACAGAGCCCGGCAACCCCGGCTGCGCAGCTGATCCTGCTGTTTCACGGTGTTGGCGA
TACTCCGGTGGCGATGGGCGAGATTGGCAGCTATTTCGCCAAAGACTTCCCGCAGGCGCAGGTAGTCAGCATTGGTGGCC
CTTTTGCCTTCGGTAACGGTGGCGGCCGCCAGTGGTTTTCGGTGCAGGGTGTGACCGAAGAAAACCGTGCCGAGCGCATT
GCTGAAGTGATGCCGAAGTTTATCGAAACCGTGCGTTACTGGCAGCAACAAAGCGGCGTGAATGACGCCGGTACCGCGCT
GGTCGGTTTTTCTCAGGGCTCAATCATGGCGCTGGAAGCCTTGAAGGTGGAACCCCGGCTGGCCGGTCGCATCGTGGCTT
TCAGCGGGCGTTTTGCCGGTTTGCCTGATAAAGCGTTCGGTGACAGCGTGGTGCATTTGATCCACGGCGAACAGGATGCG
GTAATTAGGGTGCAACATGCCAGGGCGGCGGCTGAGCGGCTGCAGGCTATCGGCGTCGATTTCACGCTGGACGTGGAAGA
AGACGTGGGGCACGCCATCAATCAGGGCATGATGAATGATGCCCTGGAACGGTTGCATTACTACGTCCCGCAACGCTACT
GGGACGAAGCGATGTTTGGCAAGCGCGGCGATTTGGTGGCCTTTAAATAA

Upstream 100 bases:

>100_bases
ATAAAACCCGATTTTCCTGAATTCTCAGCGGCGTATAGTAGCTGGCAGGAACCGGGGCATCTGGCGCCGGGCAGAATTTG
ATCAACACGAGGAGTCACCG

Downstream 100 bases:

>100_bases
ACTTCAGGGCGCTTATACCCGTCGCCTTTCAAGTTGCAGCGTTGTTACCTGCACTCGCTCACCCCAGTTACTTACTCGAG
TAAGCGCCTGGGGATGAGCG

Product: esterase YpfH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKHEHFVVQSPATPAAQLILLFHGVGDTPVAMGEIGSYFAKDFPQAQVVSIGGPFAFGNGGGRQWFSVQGVTEENRAERI
AEVMPKFIETVRYWQQQSGVNDAGTALVGFSQGSIMALEALKVEPRLAGRIVAFSGRFAGLPDKAFGDSVVHLIHGEQDA
VIRVQHARAAAERLQAIGVDFTLDVEEDVGHAINQGMMNDALERLHYYVPQRYWDEAMFGKRGDLVAFK

Sequences:

>Translated_229_residues
MKHEHFVVQSPATPAAQLILLFHGVGDTPVAMGEIGSYFAKDFPQAQVVSIGGPFAFGNGGGRQWFSVQGVTEENRAERI
AEVMPKFIETVRYWQQQSGVNDAGTALVGFSQGSIMALEALKVEPRLAGRIVAFSGRFAGLPDKAFGDSVVHLIHGEQDA
VIRVQHARAAAERLQAIGVDFTLDVEEDVGHAINQGMMNDALERLHYYVPQRYWDEAMFGKRGDLVAFK
>Mature_229_residues
MKHEHFVVQSPATPAAQLILLFHGVGDTPVAMGEIGSYFAKDFPQAQVVSIGGPFAFGNGGGRQWFSVQGVTEENRAERI
AEVMPKFIETVRYWQQQSGVNDAGTALVGFSQGSIMALEALKVEPRLAGRIVAFSGRFAGLPDKAFGDSVVHLIHGEQDA
VIRVQHARAAAERLQAIGVDFTLDVEEDVGHAINQGMMNDALERLHYYVPQRYWDEAMFGKRGDLVAFK

Specific function: Displays esterase activity toward palmitoyl-CoA and pNP- butyrate [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI87082111, Length=225, Percent_Identity=60.4444444444444, Blast_Score=277, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 25073; Mature: 25073

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHEHFVVQSPATPAAQLILLFHGVGDTPVAMGEIGSYFAKDFPQAQVVSIGGPFAFGNG
CCCCCEEEECCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEECCC
GGRQWFSVQGVTEENRAERIAEVMPKFIETVRYWQQQSGVNDAGTALVGFSQGSIMALEA
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEE
LKVEPRLAGRIVAFSGRFAGLPDKAFGDSVVHLIHGEQDAVIRVQHARAAAERLQAIGVD
EECCCHHHCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHCCC
FTLDVEEDVGHAINQGMMNDALERLHYYVPQRYWDEAMFGKRGDLVAFK
EEEECHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MKHEHFVVQSPATPAAQLILLFHGVGDTPVAMGEIGSYFAKDFPQAQVVSIGGPFAFGNG
CCCCCEEEECCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEECCC
GGRQWFSVQGVTEENRAERIAEVMPKFIETVRYWQQQSGVNDAGTALVGFSQGSIMALEA
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEE
LKVEPRLAGRIVAFSGRFAGLPDKAFGDSVVHLIHGEQDAVIRVQHARAAAERLQAIGVD
EECCCHHHCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHCCC
FTLDVEEDVGHAINQGMMNDALERLHYYVPQRYWDEAMFGKRGDLVAFK
EEEECHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]