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The map label for this gene is napA

Identifier: 157371723

GI number: 157371723

Start: 3858709

End: 3861195

Strand: Reverse

Name: napA

Synonym: Spro_3486

Alternate gene names: 157371723

Gene position: 3861195-3858709 (Counterclockwise)

Preceding gene: 157371724

Following gene: 157371722

Centisome position: 70.86

GC content: 58.83

Gene sequence:

>2487_bases
ATGAAACTCAGTCGTCGAGACTTCATGAAAGCAAACGCCGCGGTCGCCGCTGCGGCAGCCGCCGGGCTGACTATTCCTAC
GGTGGTGCAGGCGGCGGCAGGCAGCGCCGACTCTATAAAATGGGACAAAGCGCCCTGTCGTTTCTGCGGCACCGGTTGTG
GCGTGCTGGTCGGTACCCAGAACGGCCGCATTGTGGCCTCACAGGGCGATCCGGAAGCGGCGGTCAACCGTGGGTTGAGC
TGCATCAAAGGCTATTTCCTGCCAAAGATCATGTACGGCAAAGATCGTCTGAATCAACCACTGCTGCGCATGAAAGACGG
CCAGTACCACAAGGAAGGCGAGTTCACGCCGGTCAGTTGGCAGCAGGCTTTCGACATCATGGCCGAAAAGTTCAAGCAGG
CCCTGAAAGAGAAAGGCCCAGAGGCCGTTGGCATGTTCGGTTCCGGTCAGTGGACGGTGTGGGAAGGCTATGCCGCCGCC
AAATTGCTCAAAGCCGGGCTGCGCTCCAATAACCTGGATCCGAATGCCCGTCACTGTATGGCGTCAGCGGTAGTGGGCTT
TATGCGCACCTTCGGCATGGACGAGCCGATGGGCTGCTATGACGATATCGAGCAGGCCGATGCCTTTGTGCTGTGGGGCT
CCAACATGGCGGAGATGCACCCGATCCTGTGGTCGCGCATCACTGACCGCCGCCTGAGCAATGAGAAGGTCAACGTATCG
GTCCTGTCGACTTTTGAGCATCGCAGCTTCGAACTGGCGGACAACGGCATGGTGTTCACGCCGCAGACCGATCTGGCGAT
CATGAACTACATCGCTAATTACATCATTCAAAACAACGCGGTAGATCAGGACTTCCTTAATCGGCACGTCAACTTCCGCC
GTGGCGCGACGGATATCGGCTATGGCCTGCGCCCAACCGACCCATTGGAAAAAGCGGCGAAAAACCCCGGCAGCGACGCT
TCGGACCCGATGAGCTTCGAAGAATACAAAGCCTTTGTCGCGGACTACACGCTGGAAAAAACGGCCAAAATGAGCGGCGT
GCCGGAAGACCAACTGGAAGCGCTGGCAAAACTGTATGCCGATCCGAACATCAAGGTGGTGTCGTACTGGACCATGGGCT
TTAACCAGCATACGCGCGGCGTATGGGCCAACAACCTGTGCTACAACCTGCATCTGTTGACCGGCAAAATCTCCAAACCG
GGCTGTGGGCCGTTCTCACTGACCGGCCAGCCTTCCGCCTGCGGCACCGCCCGTGAAGTGGGCACCTTTGCCCACCGTCT
GCCGGCCGACATGGTGGTCACCAACGAAAAACATCGGCAGACCGCCGAGCAGAAGTGGCAGCTGCCGGCCGGTACCATTC
CAGCCAAAGTCGGGTTGCATGCGGTAGCGCAGGATCGCGCGCTGAAAGACGGCACGCTGAACGCTTACTGGGTGATGTGC
AACAACAACATGCAGGCCGGGCCGAACATCAATGAAGACCGGATGCCGGGCTGGCGTGATGCACGCAACTTTGTGGTGGT
GTCGGATCCTTACCCGACCGTCAGCGCCCTGGCAGCCGATCTGATCCTGCCGACCGCCATGTGGGTGGAAAAAGAGGGGG
CCTACGGTAATGCGGAGCGCCGTACTCAGTTCTGGCGCCAGCAGGTGAAAGCGCCGGGCGAAGCCAAATCCGATCTGTGG
CAACTGGTGGAGTTCTCCAAACGCTTTACGGTGGAAGAAGTGTGGCCGGCCGAATTACTGGCACAGAAACCGGAATATCG
CGGTAAAACGCTGTACGACGTGCTGTTTGCCAACGGTGAGGTCAATAAATACCCGCTGACGGAGATCCCGGCAGATCAGC
TTAACGATGAAGCTCGCGACTTCGGCTTCTATCTGCAAAAAGGGCTGTTTGAAGAATATGCCGGCTTTGGTCGCGGTCAT
GGGCACGATCTGGCGCCGTTCGATAGCTATCATCAGGCTCGAGGTCTGCGCTGGCCGGTGGTGGAGGGCAAAGAGACGTT
GTGGCGCTACCGCGAAGGTACTGACCCCTACGTTAAAGCCGGAGAAGAAGTGCGTTTCTACGGCAAGCCGGACGGCAAGG
CGGTGATCTTCGCGCTGCCATTCGAACCGGCGGCGGAAAGCCCGGATAAAGAATATGACCTTTGGTTATCCACCGGTCGG
GTGCTGGAACATTGGCATACCGGTTCGATGACGCGCCGGGTACCCGAGCTGCACCGTGCCTTCCCGCAGGCGGTGGTGTT
TATCCATCCGCTGGACGCCAAAAGCCGCAACCTGCGCCGTGGCGAGAAGGTCAGGGTGCTGTCGCGCCGTGGCGAATTGA
TCAGCACGGTCGAAACTCGCGGCCGCAACCGTCCGCCAAGAGGGCTGGTGTATATGCCGTTCTTTGATGCGGCGCAGCTG
GTCAATAACCTGACGCTGGACGCCACCGATCCGCTTTCCAAAGAGGTCGATTTCAAGAAATGCGCGGTGAAGTTGGAGAA
GGTGTGA

Upstream 100 bases:

>100_bases
ATCAGCTGCTGGAAAAAATTGAGTCGGTACGCAATTTGGATGGCGTGCTGGCGGTATCGCTGGTTTATCACCAGCAGGAC
ACTCAAGGTGAGGTAACGCC

Downstream 100 bases:

>100_bases
GGCTGACGAATTGCGCGATGAACACCTTATTTAATGACCCGGGAGCCAAATAAAAATGAAAAGCCCTGTTGTGAAAAAGA
CGTTTGCCCTGTGGGTGACC

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 828; Mature: 828

Protein sequence:

>828_residues
MKLSRRDFMKANAAVAAAAAAGLTIPTVVQAAAGSADSIKWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPEAAVNRGLS
CIKGYFLPKIMYGKDRLNQPLLRMKDGQYHKEGEFTPVSWQQAFDIMAEKFKQALKEKGPEAVGMFGSGQWTVWEGYAAA
KLLKAGLRSNNLDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITDRRLSNEKVNVS
VLSTFEHRSFELADNGMVFTPQTDLAIMNYIANYIIQNNAVDQDFLNRHVNFRRGATDIGYGLRPTDPLEKAAKNPGSDA
SDPMSFEEYKAFVADYTLEKTAKMSGVPEDQLEALAKLYADPNIKVVSYWTMGFNQHTRGVWANNLCYNLHLLTGKISKP
GCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRQTAEQKWQLPAGTIPAKVGLHAVAQDRALKDGTLNAYWVMC
NNNMQAGPNINEDRMPGWRDARNFVVVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVKAPGEAKSDLW
QLVEFSKRFTVEEVWPAELLAQKPEYRGKTLYDVLFANGEVNKYPLTEIPADQLNDEARDFGFYLQKGLFEEYAGFGRGH
GHDLAPFDSYHQARGLRWPVVEGKETLWRYREGTDPYVKAGEEVRFYGKPDGKAVIFALPFEPAAESPDKEYDLWLSTGR
VLEHWHTGSMTRRVPELHRAFPQAVVFIHPLDAKSRNLRRGEKVRVLSRRGELISTVETRGRNRPPRGLVYMPFFDAAQL
VNNLTLDATDPLSKEVDFKKCAVKLEKV

Sequences:

>Translated_828_residues
MKLSRRDFMKANAAVAAAAAAGLTIPTVVQAAAGSADSIKWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPEAAVNRGLS
CIKGYFLPKIMYGKDRLNQPLLRMKDGQYHKEGEFTPVSWQQAFDIMAEKFKQALKEKGPEAVGMFGSGQWTVWEGYAAA
KLLKAGLRSNNLDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITDRRLSNEKVNVS
VLSTFEHRSFELADNGMVFTPQTDLAIMNYIANYIIQNNAVDQDFLNRHVNFRRGATDIGYGLRPTDPLEKAAKNPGSDA
SDPMSFEEYKAFVADYTLEKTAKMSGVPEDQLEALAKLYADPNIKVVSYWTMGFNQHTRGVWANNLCYNLHLLTGKISKP
GCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRQTAEQKWQLPAGTIPAKVGLHAVAQDRALKDGTLNAYWVMC
NNNMQAGPNINEDRMPGWRDARNFVVVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVKAPGEAKSDLW
QLVEFSKRFTVEEVWPAELLAQKPEYRGKTLYDVLFANGEVNKYPLTEIPADQLNDEARDFGFYLQKGLFEEYAGFGRGH
GHDLAPFDSYHQARGLRWPVVEGKETLWRYREGTDPYVKAGEEVRFYGKPDGKAVIFALPFEPAAESPDKEYDLWLSTGR
VLEHWHTGSMTRRVPELHRAFPQAVVFIHPLDAKSRNLRRGEKVRVLSRRGELISTVETRGRNRPPRGLVYMPFFDAAQL
VNNLTLDATDPLSKEVDFKKCAVKLEKV
>Mature_828_residues
MKLSRRDFMKANAAVAAAAAAGLTIPTVVQAAAGSADSIKWDKAPCRFCGTGCGVLVGTQNGRIVASQGDPEAAVNRGLS
CIKGYFLPKIMYGKDRLNQPLLRMKDGQYHKEGEFTPVSWQQAFDIMAEKFKQALKEKGPEAVGMFGSGQWTVWEGYAAA
KLLKAGLRSNNLDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITDRRLSNEKVNVS
VLSTFEHRSFELADNGMVFTPQTDLAIMNYIANYIIQNNAVDQDFLNRHVNFRRGATDIGYGLRPTDPLEKAAKNPGSDA
SDPMSFEEYKAFVADYTLEKTAKMSGVPEDQLEALAKLYADPNIKVVSYWTMGFNQHTRGVWANNLCYNLHLLTGKISKP
GCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRQTAEQKWQLPAGTIPAKVGLHAVAQDRALKDGTLNAYWVMC
NNNMQAGPNINEDRMPGWRDARNFVVVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVKAPGEAKSDLW
QLVEFSKRFTVEEVWPAELLAQKPEYRGKTLYDVLFANGEVNKYPLTEIPADQLNDEARDFGFYLQKGLFEEYAGFGRGH
GHDLAPFDSYHQARGLRWPVVEGKETLWRYREGTDPYVKAGEEVRFYGKPDGKAVIFALPFEPAAESPDKEYDLWLSTGR
VLEHWHTGSMTRRVPELHRAFPQAVVFIHPLDAKSRNLRRGEKVRVLSRRGELISTVETRGRNRPPRGLVYMPFFDAAQL
VNNLTLDATDPLSKEVDFKKCAVKLEKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=828, Percent_Identity=83.9371980676328, Blast_Score=1494, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=559, Percent_Identity=26.4758497316637, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI87081797, Length=888, Percent_Identity=24.0990990990991, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI3868720, Length=661, Percent_Identity=22.9954614220877, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1787870, Length=879, Percent_Identity=22.8668941979522, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI3868719, Length=488, Percent_Identity=23.3606557377049, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI171474008, Length=888, Percent_Identity=22.0720720720721, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_SERP5 (A8GHJ4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479712.1
- ProteinModelPortal:   A8GHJ4
- SMR:   A8GHJ4
- STRING:   A8GHJ4
- GeneID:   5604924
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3486
- eggNOG:   COG0243
- HOGENOM:   HBG715033
- OMA:   NMLYNIH
- ProtClustDB:   PRK13532
- BioCyc:   SPRO399741:SPRO_3486-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 92600; Mature: 92600

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRRDFMKANAAVAAAAAAGLTIPTVVQAAAGSADSIKWDKAPCRFCGTGCGVLVGTQ
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEECC
NGRIVASQGDPEAAVNRGLSCIKGYFLPKIMYGKDRLNQPLLRMKDGQYHKEGEFTPVSW
CCEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCCCCCCCCCCCCH
QQAFDIMAEKFKQALKEKGPEAVGMFGSGQWTVWEGYAAAKLLKAGLRSNNLDPNARHCM
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHH
ASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITDRRLSNEKVNVS
HHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEE
VLSTFEHRSFELADNGMVFTPQTDLAIMNYIANYIIQNNAVDQDFLNRHVNFRRGATDIG
EEECCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCC
YGLRPTDPLEKAAKNPGSDASDPMSFEEYKAFVADYTLEKTAKMSGVPEDQLEALAKLYA
CCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNIKVVSYWTMGFNQHTRGVWANNLCYNLHLLTGKISKPGCGPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCCCCCCCEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
GTFAHRLPADMVVTNEKHRQTAEQKWQLPAGTIPAKVGLHAVAQDRALKDGTLNAYWVMC
HHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEE
NNNMQAGPNINEDRMPGWRDARNFVVVSDPYPTVSALAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHH
RTQFWRQQVKAPGEAKSDLWQLVEFSKRFTVEEVWPAELLAQKPEYRGKTLYDVLFANGE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCCHHHHHCCCCCCCCEEEEEEEECCC
VNKYPLTEIPADQLNDEARDFGFYLQKGLFEEYAGFGRGHGHDLAPFDSYHQARGLRWPV
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCC
VEGKETLWRYREGTDPYVKAGEEVRFYGKPDGKAVIFALPFEPAAESPDKEYDLWLSTGR
CCCHHHHHHHHCCCCHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCH
VLEHWHTGSMTRRVPELHRAFPQAVVFIHPLDAKSRNLRRGEKVRVLSRRGELISTVETR
HHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHC
GRNRPPRGLVYMPFFDAAQLVNNLTLDATDPLSKEVDFKKCAVKLEKV
CCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKLSRRDFMKANAAVAAAAAAGLTIPTVVQAAAGSADSIKWDKAPCRFCGTGCGVLVGTQ
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCEEEEECC
NGRIVASQGDPEAAVNRGLSCIKGYFLPKIMYGKDRLNQPLLRMKDGQYHKEGEFTPVSW
CCEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCCCCCCCCCCCCH
QQAFDIMAEKFKQALKEKGPEAVGMFGSGQWTVWEGYAAAKLLKAGLRSNNLDPNARHCM
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHH
ASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAEMHPILWSRITDRRLSNEKVNVS
HHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEE
VLSTFEHRSFELADNGMVFTPQTDLAIMNYIANYIIQNNAVDQDFLNRHVNFRRGATDIG
EEECCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCC
YGLRPTDPLEKAAKNPGSDASDPMSFEEYKAFVADYTLEKTAKMSGVPEDQLEALAKLYA
CCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
DPNIKVVSYWTMGFNQHTRGVWANNLCYNLHLLTGKISKPGCGPFSLTGQPSACGTAREV
CCCEEEEEEEEECCCCCCCCCEECCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
GTFAHRLPADMVVTNEKHRQTAEQKWQLPAGTIPAKVGLHAVAQDRALKDGTLNAYWVMC
HHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEE
NNNMQAGPNINEDRMPGWRDARNFVVVSDPYPTVSALAADLILPTAMWVEKEGAYGNAER
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHH
RTQFWRQQVKAPGEAKSDLWQLVEFSKRFTVEEVWPAELLAQKPEYRGKTLYDVLFANGE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCCHHHHHCCCCCCCCEEEEEEEECCC
VNKYPLTEIPADQLNDEARDFGFYLQKGLFEEYAGFGRGHGHDLAPFDSYHQARGLRWPV
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCC
VEGKETLWRYREGTDPYVKAGEEVRFYGKPDGKAVIFALPFEPAAESPDKEYDLWLSTGR
CCCHHHHHHHHCCCCHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCH
VLEHWHTGSMTRRVPELHRAFPQAVVFIHPLDAKSRNLRRGEKVRVLSRRGELISTVETR
HHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHC
GRNRPPRGLVYMPFFDAAQLVNNLTLDATDPLSKEVDFKKCAVKLEKV
CCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA