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The map label for this gene is budB [H]

Identifier: 157371669

GI number: 157371669

Start: 3802451

End: 3804136

Strand: Reverse

Name: budB [H]

Synonym: Spro_3432

Alternate gene names: 157371669

Gene position: 3804136-3802451 (Counterclockwise)

Preceding gene: 157371670

Following gene: 157371668

Centisome position: 69.82

GC content: 58.78

Gene sequence:

>1686_bases
ATGAACAAGGCAAAAACAGACAATAACTGGGCATGTGGCGCAGATTTAGTGGTGAAAAATCTGGAGGCGCAGGGGGTTAA
GCACGTATTCGGCATCCCGGGGGCCAAGATTGACCGGGTATTTGATTCTCTGGAGGATGCGCCGTCGATAGAAACGGTGG
TGGTGCGTCACGAAGCCAACGCGGCATTTATGGCGGCGGCGGTCGGGCGCCTGACCGGCAAGGCCGGGGTGGCGCTGGTG
ACCTCCGGGCCGGGCAGCTCTAACCTGATCACCGGGCTGGCGACCGCCACCTCGGAAGGGGATGCGGTAGTGGCTTTTGG
CGGCGCGGTGAAACGAGCCGATAACCTGAAACAGACTCACCAGAGCATGGACACCGTCAGCATGTTCCGGCCAGTGACCA
AATACTGTGCCGAGGTGCATGCCGGTTCAGCGATTTCAGAAGTGATCGCCAATGCCTTCCGCCACGCCGAGTTCGGCCGT
CCGGGGGCCTCTTTTATCAGTCTGCCAATGGATATCGTCAATGAGCCGGTCAGTGCGCCGGTGCTGGCGGGATGCCGGTT
GCCACGCATGGGGGCGGCGGCGGCCGAGGATATTCGCGCCGCGGTCAAATTGATCCAGCAGGCAAAATGCCCGGTATTGC
TGTTGGGTTTGCAGGCCAGCCGTCCGGAAAACAGCGAGTCGGTACGTCAGTTGCTGCATCGCACCCATATGCCGGTGGTG
GGCACTTATCAGGCGGCGGGGGTGATAGACGTTAACCACTTTGCGCGCTTTGCCGGGCGCGTTGGCTTGTTCAATAACCA
ACCCGCCGACCAGCTATTGCAAAAGGCCGATCTGGTGGTCAGCGTCGGTTATGGCCCAATTGAATACGATCCCTGCATGT
GGAATAGCCAGGGCCGGTTGAAACTGGTGCATATCGACGTGCTGCCGGCGGATATAGACACCTGCTATCGCCCGGACGTC
GAGCTGGTGGGCAATATTAGCGCCACTCTCGACATGATGACCGAAGCCTTTAGCGGAGCGGTGAGCGTACCGACGGAGGT
GGAATTGATCCTCAGCGATCTGGGCCGTCAGCGCCATGAACTGGCGGAACGGGCAGCCCGGCGTGGCGGCATGCCAATTC
ACCCGTTGCGCATCGTGAAAGAGCTGCAGGATATCGTCAGCGACGACGTGACGCTGTGCGTGGACATGGGCAGTTTCCAC
ATCTGGATTGCACGTTATCTTTACAGTTTCCGCGCGCGCCAGTTACTGATTTCCAACGGCCAACAGACCATGGGGGTAGC
GTTGCCGTGGGCGATCGGTGCGGCGCTGGTGCGCCCGGGGGACAAAGTGGTCTCTATCTCCGGCGACGGCGGTTTTATGC
AGTCGAGCATGGAGCTGGAAACCGCAGTGCGCCTGAAAAATAACATCGTGCACGTCATTTGGGTGGATAACGCCTACAAC
ATGGTGGAAATGCAGGAGGTGAACAAATACCGGCGCAAATCCGGGGTGGAATTCGGCCCGATTGATTTTAAAGCCTATGC
TGAAGCCTGCGGCGCGGTCGGGTTTGCCGTGCAGTCGGTGGAAGACTTGCGACCGATGCTGCGTAAGGCAATGGAAATTC
AGGGGCCGGTGGTGGTGGCTATTCCGGTGGATTACGCCGACAACTACAAACTGATGGCGCAGATGAATTTCAGCCAGATG
ATTTAA

Upstream 100 bases:

>100_bases
TCTGCGCGCCGACCTCTGTCCAGAGGATTTGGATCGCGCAATTCGTTCTGCCGAGGGTTAATTCCGCGACAGTCTTGGGT
TAGTTTTCAGGAGGTATTCC

Downstream 100 bases:

>100_bases
TTTCAGCAAGTCGCTAAACCACGGGGAATGCCGCATGAGGCACTCCCCGTTTTTCATGCTCAGAATTTGTGCTTTACATC
CCTAACTGCATGATTTATTG

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MNKAKTDNNWACGADLVVKNLEAQGVKHVFGIPGAKIDRVFDSLEDAPSIETVVVRHEANAAFMAAAVGRLTGKAGVALV
TSGPGSSNLITGLATATSEGDAVVAFGGAVKRADNLKQTHQSMDTVSMFRPVTKYCAEVHAGSAISEVIANAFRHAEFGR
PGASFISLPMDIVNEPVSAPVLAGCRLPRMGAAAAEDIRAAVKLIQQAKCPVLLLGLQASRPENSESVRQLLHRTHMPVV
GTYQAAGVIDVNHFARFAGRVGLFNNQPADQLLQKADLVVSVGYGPIEYDPCMWNSQGRLKLVHIDVLPADIDTCYRPDV
ELVGNISATLDMMTEAFSGAVSVPTEVELILSDLGRQRHELAERAARRGGMPIHPLRIVKELQDIVSDDVTLCVDMGSFH
IWIARYLYSFRARQLLISNGQQTMGVALPWAIGAALVRPGDKVVSISGDGGFMQSSMELETAVRLKNNIVHVIWVDNAYN
MVEMQEVNKYRRKSGVEFGPIDFKAYAEACGAVGFAVQSVEDLRPMLRKAMEIQGPVVVAIPVDYADNYKLMAQMNFSQM
I

Sequences:

>Translated_561_residues
MNKAKTDNNWACGADLVVKNLEAQGVKHVFGIPGAKIDRVFDSLEDAPSIETVVVRHEANAAFMAAAVGRLTGKAGVALV
TSGPGSSNLITGLATATSEGDAVVAFGGAVKRADNLKQTHQSMDTVSMFRPVTKYCAEVHAGSAISEVIANAFRHAEFGR
PGASFISLPMDIVNEPVSAPVLAGCRLPRMGAAAAEDIRAAVKLIQQAKCPVLLLGLQASRPENSESVRQLLHRTHMPVV
GTYQAAGVIDVNHFARFAGRVGLFNNQPADQLLQKADLVVSVGYGPIEYDPCMWNSQGRLKLVHIDVLPADIDTCYRPDV
ELVGNISATLDMMTEAFSGAVSVPTEVELILSDLGRQRHELAERAARRGGMPIHPLRIVKELQDIVSDDVTLCVDMGSFH
IWIARYLYSFRARQLLISNGQQTMGVALPWAIGAALVRPGDKVVSISGDGGFMQSSMELETAVRLKNNIVHVIWVDNAYN
MVEMQEVNKYRRKSGVEFGPIDFKAYAEACGAVGFAVQSVEDLRPMLRKAMEIQGPVVVAIPVDYADNYKLMAQMNFSQM
I
>Mature_561_residues
MNKAKTDNNWACGADLVVKNLEAQGVKHVFGIPGAKIDRVFDSLEDAPSIETVVVRHEANAAFMAAAVGRLTGKAGVALV
TSGPGSSNLITGLATATSEGDAVVAFGGAVKRADNLKQTHQSMDTVSMFRPVTKYCAEVHAGSAISEVIANAFRHAEFGR
PGASFISLPMDIVNEPVSAPVLAGCRLPRMGAAAAEDIRAAVKLIQQAKCPVLLLGLQASRPENSESVRQLLHRTHMPVV
GTYQAAGVIDVNHFARFAGRVGLFNNQPADQLLQKADLVVSVGYGPIEYDPCMWNSQGRLKLVHIDVLPADIDTCYRPDV
ELVGNISATLDMMTEAFSGAVSVPTEVELILSDLGRQRHELAERAARRGGMPIHPLRIVKELQDIVSDDVTLCVDMGSFH
IWIARYLYSFRARQLLISNGQQTMGVALPWAIGAALVRPGDKVVSISGDGGFMQSSMELETAVRLKNNIVHVIWVDNAYN
MVEMQEVNKYRRKSGVEFGPIDFKAYAEACGAVGFAVQSVEDLRPMLRKAMEIQGPVVVAIPVDYADNYKLMAQMNFSQM
I

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=26.7379679144385, Blast_Score=176, Evalue=8e-44,
Organism=Homo sapiens, GI21361361, Length=548, Percent_Identity=25.3649635036496, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI87081685, Length=536, Percent_Identity=29.4776119402985, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1790104, Length=544, Percent_Identity=29.0441176470588, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786717, Length=571, Percent_Identity=26.6199649737303, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787096, Length=532, Percent_Identity=26.3157894736842, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=23.3928571428571, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=556, Percent_Identity=26.7985611510791, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=556, Percent_Identity=26.7985611510791, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=579, Percent_Identity=25.9067357512953, Blast_Score=129, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=561, Percent_Identity=27.0944741532977, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320816, Length=552, Percent_Identity=24.2753623188406, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=474, Percent_Identity=22.9957805907173, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=456, Percent_Identity=23.4649122807018, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922626, Length=477, Percent_Identity=25.5765199161426, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60656; Mature: 60656

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAKTDNNWACGADLVVKNLEAQGVKHVFGIPGAKIDRVFDSLEDAPSIETVVVRHEAN
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCC
AAFMAAAVGRLTGKAGVALVTSGPGSSNLITGLATATSEGDAVVAFGGAVKRADNLKQTH
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHH
QSMDTVSMFRPVTKYCAEVHAGSAISEVIANAFRHAEFGRPGASFISLPMDIVNEPVSAP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCCC
VLAGCRLPRMGAAAAEDIRAAVKLIQQAKCPVLLLGLQASRPENSESVRQLLHRTHMPVV
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEE
GTYQAAGVIDVNHFARFAGRVGLFNNQPADQLLQKADLVVSVGYGPIEYDPCMWNSQGRL
EEECCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEECCCCCE
KLVHIDVLPADIDTCYRPDVELVGNISATLDMMTEAFSGAVSVPTEVELILSDLGRQRHE
EEEEEEEECCCHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHH
LAERAARRGGMPIHPLRIVKELQDIVSDDVTLCVDMGSFHIWIARYLYSFRARQLLISNG
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
QQTMGVALPWAIGAALVRPGDKVVSISGDGGFMQSSMELETAVRLKNNIVHVIWVDNAYN
CHHEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
MVEMQEVNKYRRKSGVEFGPIDFKAYAEACGAVGFAVQSVEDLRPMLRKAMEIQGPVVVA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IPVDYADNYKLMAQMNFSQMI
EECCCCCCEEEEEECCHHHCC
>Mature Secondary Structure
MNKAKTDNNWACGADLVVKNLEAQGVKHVFGIPGAKIDRVFDSLEDAPSIETVVVRHEAN
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCC
AAFMAAAVGRLTGKAGVALVTSGPGSSNLITGLATATSEGDAVVAFGGAVKRADNLKQTH
HHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHH
QSMDTVSMFRPVTKYCAEVHAGSAISEVIANAFRHAEFGRPGASFISLPMDIVNEPVSAP
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCCC
VLAGCRLPRMGAAAAEDIRAAVKLIQQAKCPVLLLGLQASRPENSESVRQLLHRTHMPVV
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEE
GTYQAAGVIDVNHFARFAGRVGLFNNQPADQLLQKADLVVSVGYGPIEYDPCMWNSQGRL
EEECCCCEEEHHHHHHHHCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCEECCCCCE
KLVHIDVLPADIDTCYRPDVELVGNISATLDMMTEAFSGAVSVPTEVELILSDLGRQRHE
EEEEEEEECCCHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHH
LAERAARRGGMPIHPLRIVKELQDIVSDDVTLCVDMGSFHIWIARYLYSFRARQLLISNG
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
QQTMGVALPWAIGAALVRPGDKVVSISGDGGFMQSSMELETAVRLKNNIVHVIWVDNAYN
CHHEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCC
MVEMQEVNKYRRKSGVEFGPIDFKAYAEACGAVGFAVQSVEDLRPMLRKAMEIQGPVVVA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
IPVDYADNYKLMAQMNFSQMI
EECCCCCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]