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The map label for this gene is fadI

Identifier: 157371616

GI number: 157371616

Start: 3739916

End: 3741226

Strand: Reverse

Name: fadI

Synonym: Spro_3379

Alternate gene names: 157371616

Gene position: 3741226-3739916 (Counterclockwise)

Preceding gene: 157371617

Following gene: 157371615

Centisome position: 68.66

GC content: 59.65

Gene sequence:

>1311_bases
ATGAGTAAGGCACTGCCGTTGGTGACCCGTCACGGTGACCGTATTGCGATTGTAAATGGACTGCGCACCCCCTTTGCCAA
ACAGGCGACCGCTTATCACGGTATTCCGGCGGTAGATTTGGGTAAAACGGCGGTGAGTGAACTGTTGGCCCGCACTGGCA
TCGATCCTGCGTTGATCGAGCAACTGGTGTTCGGCCAGGTGGTGCAAATGCCTGAAGCGCCGAATATTGCGCGTGAAATC
GTGCTCGGTACCGGCATGAGCGTGCATACCGATGCCTACAGCGTGTCGCGTGCCTGCGCCACCAGTTTCCAGGCGATTGC
CAACGTGGCGGAAAGCATCATGGCCGGTAGCATCAGCATCGGTATTGCCGGCGGCGCTGACTCTTCTTCCGTTTTGCCTA
TTGGCGTCAGCAAAGCGCTGGCACGCACCCTGGTGGACGTTAACAAGGCCCGAACCCTGTCACAGCGCCTCAAATTGTTC
AGCCGACTGAAGTTCCGTGACCTGATGCCGGTTCCCCCGGCGGTAGCGGAATACTCTACGGGTCTGCGAATGGGCGATAC
CGCCGAACAAATGGCGAAAAGCCACGGCATCACCCGTGAAGAGCAGGATGCGTTGGCGCACCGCTCGCATCAGTTAGCGG
CCAAAGCCTGGGAACAGGGGCTGCTGCACGATGAGGTGATGACCGCCTACGTGCCGCCGTATCGCACCCAAATTAGCGAA
GACAACAACGTCCGCAAAGACTCCAGCCTGGCTTCCTACGCGAAGCTGAAACCGGCATTTGACCGCAAGCATGGCAGCGT
TACCGCAGCCAACAGCACCCCCCTGACCGACGGTGCGGCGGCGGTACTGATGATGAGTGAATCGCGGGCGAAAGAGCTGG
GCTTACAACCGCTGGGCTACCTGCGCAGCTTTGCCTTCTCCGCCATCGACGTGTGGGAAGATATGTTGCTTGGGCCGTCC
TACGCCACGCCGCTGGCGTTGGATCGCGCCGGTATCGGCCTGGCGGATCTGACGCTTATCGACATGCATGAAGCTTTCGC
CGCACAAACCCTGGCCAACCTGAAGATGTTTGCCAGTGACGAGTTTGCCCAGCAAAAATTGGGTCGCAGCCGGGCGATTG
GTGAAGTGGATATGGATAAATTCAACGTATTGGGCGGCTCGATCGCCTATGGCCACCCGTTTGCCGCTACCGGCGCGCGC
ATGATCACCCAAACGCTGCACGAGCTGAAACGCCGTGGTGGCGGGCTGGGGTTGACCACCGCCTGCGCGGCCGGAGGGTT
AGGGGCCGCAATGATCGTAGAGGTGGAATAA

Upstream 100 bases:

>100_bases
TCTGACGGTTTGCCAAATGTAAACATTTGGTTAACAATGGCCCCATCAGGTCAGACCTCTTTTGGCCGTTGTGCTGACGT
TATTCTCTTCAGGAGCGTTT

Downstream 100 bases:

>100_bases
TGAGTTTTGAAAACGCATTGCACGAACAGCGAGCCAAACCCTCAGCCTTTCAGCTGACGATACGTCCGGACAACATAGGC
GTGATCACCATTGATGTCCC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSKALPLVTRHGDRIAIVNGLRTPFAKQATAYHGIPAVDLGKTAVSELLARTGIDPALIEQLVFGQVVQMPEAPNIAREI
VLGTGMSVHTDAYSVSRACATSFQAIANVAESIMAGSISIGIAGGADSSSVLPIGVSKALARTLVDVNKARTLSQRLKLF
SRLKFRDLMPVPPAVAEYSTGLRMGDTAEQMAKSHGITREEQDALAHRSHQLAAKAWEQGLLHDEVMTAYVPPYRTQISE
DNNVRKDSSLASYAKLKPAFDRKHGSVTAANSTPLTDGAAAVLMMSESRAKELGLQPLGYLRSFAFSAIDVWEDMLLGPS
YATPLALDRAGIGLADLTLIDMHEAFAAQTLANLKMFASDEFAQQKLGRSRAIGEVDMDKFNVLGGSIAYGHPFAATGAR
MITQTLHELKRRGGGLGLTTACAAGGLGAAMIVEVE

Sequences:

>Translated_436_residues
MSKALPLVTRHGDRIAIVNGLRTPFAKQATAYHGIPAVDLGKTAVSELLARTGIDPALIEQLVFGQVVQMPEAPNIAREI
VLGTGMSVHTDAYSVSRACATSFQAIANVAESIMAGSISIGIAGGADSSSVLPIGVSKALARTLVDVNKARTLSQRLKLF
SRLKFRDLMPVPPAVAEYSTGLRMGDTAEQMAKSHGITREEQDALAHRSHQLAAKAWEQGLLHDEVMTAYVPPYRTQISE
DNNVRKDSSLASYAKLKPAFDRKHGSVTAANSTPLTDGAAAVLMMSESRAKELGLQPLGYLRSFAFSAIDVWEDMLLGPS
YATPLALDRAGIGLADLTLIDMHEAFAAQTLANLKMFASDEFAQQKLGRSRAIGEVDMDKFNVLGGSIAYGHPFAATGAR
MITQTLHELKRRGGGLGLTTACAAGGLGAAMIVEVE
>Mature_435_residues
SKALPLVTRHGDRIAIVNGLRTPFAKQATAYHGIPAVDLGKTAVSELLARTGIDPALIEQLVFGQVVQMPEAPNIAREIV
LGTGMSVHTDAYSVSRACATSFQAIANVAESIMAGSISIGIAGGADSSSVLPIGVSKALARTLVDVNKARTLSQRLKLFS
RLKFRDLMPVPPAVAEYSTGLRMGDTAEQMAKSHGITREEQDALAHRSHQLAAKAWEQGLLHDEVMTAYVPPYRTQISED
NNVRKDSSLASYAKLKPAFDRKHGSVTAANSTPLTDGAAAVLMMSESRAKELGLQPLGYLRSFAFSAIDVWEDMLLGPSY
ATPLALDRAGIGLADLTLIDMHEAFAAQTLANLKMFASDEFAQQKLGRSRAIGEVDMDKFNVLGGSIAYGHPFAATGARM
ITQTLHELKRRGGGLGLTTACAAGGLGAAMIVEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=42.6190476190476, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI4557237, Length=423, Percent_Identity=30.0236406619385, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI4501853, Length=437, Percent_Identity=32.2654462242563, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI167614485, Length=423, Percent_Identity=30.9692671394799, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI148539872, Length=427, Percent_Identity=30.4449648711944, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=80.2752293577982, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=427, Percent_Identity=34.192037470726, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=34.9881796690307, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI48994986, Length=432, Percent_Identity=33.7962962962963, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787663, Length=424, Percent_Identity=32.5471698113208, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=38.9548693586698, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=31.924882629108, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25147385, Length=421, Percent_Identity=29.2161520190024, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17535921, Length=426, Percent_Identity=29.3427230046948, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=420, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325229, Length=436, Percent_Identity=31.1926605504587, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322031, Length=425, Percent_Identity=29.4117647058824, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=41.7249417249417, Blast_Score=327, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=29.3838862559242, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=28.4037558685446, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17648125, Length=429, Percent_Identity=30.3030303030303, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADI_SERP5 (A8GH87)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479605.1
- STRING:   A8GH87
- GeneID:   5603341
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3379
- eggNOG:   COG0183
- HOGENOM:   HBG370930
- OMA:   RDEVMTA
- ProtClustDB:   PRK08963
- BioCyc:   SPRO399741:SPRO_3379-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01618
- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930
- TIGRFAMs:   TIGR02446

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.16

Molecular weight: Translated: 46314; Mature: 46183

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 99-99 ACT_SITE 392-392 ACT_SITE 422-422

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALPLVTRHGDRIAIVNGLRTPFAKQATAYHGIPAVDLGKTAVSELLARTGIDPALIE
CCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMSVHTDAYSVSRACATSFQAIANVAESIMAGSISI
HHHHHHHHCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
GIAGGADSSSVLPIGVSKALARTLVDVNKARTLSQRLKLFSRLKFRDLMPVPPAVAEYST
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
GLRMGDTAEQMAKSHGITREEQDALAHRSHQLAAKAWEQGLLHDEVMTAYVPPYRTQISE
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCC
DNNVRKDSSLASYAKLKPAFDRKHGSVTAANSTPLTDGAAAVLMMSESRAKELGLQPLGY
CCCCCCHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCCEEEEECHHHHHHCCCCHHHH
LRSFAFSAIDVWEDMLLGPSYATPLALDRAGIGLADLTLIDMHEAFAAQTLANLKMFASD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFAQQKLGRSRAIGEVDMDKFNVLGGSIAYGHPFAATGARMITQTLHELKRRGGGLGLTT
HHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMIVEVE
HHHCCCCCEEEEEEEC
>Mature Secondary Structure 
SKALPLVTRHGDRIAIVNGLRTPFAKQATAYHGIPAVDLGKTAVSELLARTGIDPALIE
CCCCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMSVHTDAYSVSRACATSFQAIANVAESIMAGSISI
HHHHHHHHCCCCCCCHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
GIAGGADSSSVLPIGVSKALARTLVDVNKARTLSQRLKLFSRLKFRDLMPVPPAVAEYST
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHC
GLRMGDTAEQMAKSHGITREEQDALAHRSHQLAAKAWEQGLLHDEVMTAYVPPYRTQISE
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCC
DNNVRKDSSLASYAKLKPAFDRKHGSVTAANSTPLTDGAAAVLMMSESRAKELGLQPLGY
CCCCCCHHHHHHHHHHCHHHHCCCCCEEECCCCCCCCCCCEEEEECHHHHHHCCCCHHHH
LRSFAFSAIDVWEDMLLGPSYATPLALDRAGIGLADLTLIDMHEAFAAQTLANLKMFASD
HHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFAQQKLGRSRAIGEVDMDKFNVLGGSIAYGHPFAATGARMITQTLHELKRRGGGLGLTT
HHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMIVEVE
HHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA