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The map label for this gene is mepA [H]

Identifier: 157371610

GI number: 157371610

Start: 3733183

End: 3734010

Strand: Reverse

Name: mepA [H]

Synonym: Spro_3373

Alternate gene names: 157371610

Gene position: 3734010-3733183 (Counterclockwise)

Preceding gene: 157371611

Following gene: 157371609

Centisome position: 68.53

GC content: 59.66

Gene sequence:

>828_bases
ATGAAAAACTGGATGTTGGGCATCCTTGCCCTGATGGCTTCGGGCTCCGCCATGGCGTTGACGCCGTGGCAGAAAATTGA
GAACCCGGTGGCCGGTTCACCGCAGGCGGTGGGCGGTTTTGCCAATGGTTGCATTATCGGTGCCCAGCCGCTGCCGTTGA
ACTCGCCGGATTATCAGGTGATGCGCCCCGATCAACGCCGCTACTTCGGCCATCCGGATCTGCTGGCGTTTATCCAGCGC
CTGAGCAGTAAGGCGAACCAAAAAAACCTCGGTACGGTACTGATCGGCGATATGGCGATGCCGGCCGGTGGGCGCTTCAG
CAGTGGCCATGCCAGCCATCAGTCAGGGCTTGATGTCGACATCTGGTTACAGTTGCCGCGTCAACGCTGGAGTGCACAGC
AATTGCTGAAGCCGCAACCGATCGATTTGGTTTCCAGTGACGGCAAGCAGGTGGTCGCACGCCAGTGGCAGCCGCAGATT
GAAACCTTGATCAAAGCGGCGGCGCAGGATCAGGAAGTGACGCGTATTTTCGTCAACCCGGCGATCAAGCAGCGCCTGTG
CCTGGATGCCGGTGCCGATCGTGACTGGTTGCACAAGGTGCGGCCGTGGTTTGGCCATCGTGCGCACATGCATGTGCGCC
TGCGTTGTCCGGCCGGTAGCCTGGAGTGTAAAGAACAGGATACCCCACCGCCGGGCGACGGCTGTGGTGCCGAACTGGCA
AGTTGGTTCATTCCGCATCAGCCGAGTGCCAAGCCGGGTAAACCGGTACCGCCGCCATTACCGCCTACCTGTCAGGCTTT
GCTGAATAACCATTTCGCTGCGGAATAA

Upstream 100 bases:

>100_bases
TTGCCGAAGCAATGATGGCGATTGTGTTGATGGATCACCTGTTGCGCCAACGTGCGCAGAACGGTGATGTGGTTTCTAAC
GTTCCACGCTGGTAATCACC

Downstream 100 bases:

>100_bases
TAAATGGATTGGTTCGTCGTTGGCCCCGAGATGCTCGGGGTTTTGTTTGTGGTGGCATTGCTGGCGGGCTTTATCGACTC
CATTGCCGGTGGGGGGGGGT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKNWMLGILALMASGSAMALTPWQKIENPVAGSPQAVGGFANGCIIGAQPLPLNSPDYQVMRPDQRRYFGHPDLLAFIQR
LSSKANQKNLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPRQRWSAQQLLKPQPIDLVSSDGKQVVARQWQPQI
ETLIKAAAQDQEVTRIFVNPAIKQRLCLDAGADRDWLHKVRPWFGHRAHMHVRLRCPAGSLECKEQDTPPPGDGCGAELA
SWFIPHQPSAKPGKPVPPPLPPTCQALLNNHFAAE

Sequences:

>Translated_275_residues
MKNWMLGILALMASGSAMALTPWQKIENPVAGSPQAVGGFANGCIIGAQPLPLNSPDYQVMRPDQRRYFGHPDLLAFIQR
LSSKANQKNLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPRQRWSAQQLLKPQPIDLVSSDGKQVVARQWQPQI
ETLIKAAAQDQEVTRIFVNPAIKQRLCLDAGADRDWLHKVRPWFGHRAHMHVRLRCPAGSLECKEQDTPPPGDGCGAELA
SWFIPHQPSAKPGKPVPPPLPPTCQALLNNHFAAE
>Mature_275_residues
MKNWMLGILALMASGSAMALTPWQKIENPVAGSPQAVGGFANGCIIGAQPLPLNSPDYQVMRPDQRRYFGHPDLLAFIQR
LSSKANQKNLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPRQRWSAQQLLKPQPIDLVSSDGKQVVARQWQPQI
ETLIKAAAQDQEVTRIFVNPAIKQRLCLDAGADRDWLHKVRPWFGHRAHMHVRLRCPAGSLECKEQDTPPPGDGCGAELA
SWFIPHQPSAKPGKPVPPPLPPTCQALLNNHFAAE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=71.031746031746, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 30135; Mature: 30135

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNWMLGILALMASGSAMALTPWQKIENPVAGSPQAVGGFANGCIIGAQPLPLNSPDYQV
CCHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCCEE
MRPDQRRYFGHPDLLAFIQRLSSKANQKNLGTVLIGDMAMPAGGRFSSGHASHQSGLDVD
ECCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPRQRWSAQQLLKPQPIDLVSSDGKQVVARQWQPQIETLIKAAAQDQEVTRIFVNP
EEEECCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHEEHCCH
AIKQRLCLDAGADRDWLHKVRPWFGHRAHMHVRLRCPAGSLECKEQDTPPPGDGCGAELA
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
SWFIPHQPSAKPGKPVPPPLPPTCQALLNNHFAAE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNWMLGILALMASGSAMALTPWQKIENPVAGSPQAVGGFANGCIIGAQPLPLNSPDYQV
CCHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCCEE
MRPDQRRYFGHPDLLAFIQRLSSKANQKNLGTVLIGDMAMPAGGRFSSGHASHQSGLDVD
ECCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPRQRWSAQQLLKPQPIDLVSSDGKQVVARQWQPQIETLIKAAAQDQEVTRIFVNP
EEEECCHHHHHHHHHCCCCCCHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCHHHEEHCCH
AIKQRLCLDAGADRDWLHKVRPWFGHRAHMHVRLRCPAGSLECKEQDTPPPGDGCGAELA
HHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
SWFIPHQPSAKPGKPVPPPLPPTCQALLNNHFAAE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA