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The map label for this gene is ilvB [H]

Identifier: 157371531

GI number: 157371531

Start: 3645290

End: 3647119

Strand: Reverse

Name: ilvB [H]

Synonym: Spro_3294

Alternate gene names: 157371531

Gene position: 3647119-3645290 (Counterclockwise)

Preceding gene: 157371533

Following gene: 157371530

Centisome position: 66.93

GC content: 58.58

Gene sequence:

>1830_bases
ATGGAGTCTCACGCAGAGAAGTTATACACCCCCGTAGCACTGGCAACTGCGGATGGTGTCAGGGTTATTGCCACGCCCGG
CACGAAAGCGGCGGAGCAAACAAACGCTGATGTGATTATCCAGGGTCTTGAAGCTCGGGGCATTCAGCACATATTCCTGG
TACCCGGCAAGCTGGTGTACCCACTGATTAAATCCATTGAACATTCAGCCATTAGCGGCATCGTCGGCGCTCATGAAACC
GCCTGCGGCTTTATGGCCGACGGCTACGCCCGCGCCAGCCGCAAGTTTGGCGTTTGTCTGGGCATCTCCGGCCCCGGCAC
CATGAATTTCCTGCCGGCGATGGCCGCGGCCCAGGCCGATAAAATCCCGGTGCTTTATCTGGCCGGCGGTATCGCCACTT
ATCATGAAGCGCAGGGCGCTTTCCAGGACGGCAGCAATAGCGGCATTGATGAGCTGACTATCGTCAAACCGCTGCTGTCC
GCCGCAATAGAAGTGAAAAATAATCTTACGTTGCAGCACGAGCTGCGGCGCAGTTTGTCCTGCCTGAACACCCAGCGTAA
AGGCCGCGCTTATTTAAGCGTACCGGTCGATATGCAAAAGAAAACGGTAGCCGGCAATTATGAAGCCAGCCCACAGCGTA
CGCCACAGTGCCGTGAAGCGGCGATCGACCAACAGGCGCTGGAGCAGGTGTTGGATGATTATCTGCTGCGCGGGCTGAAC
GTTGCCTGCCTGGTGGGTAACCGCATGAATAATCCGTTGGATGCCGCACTGCTGCTGCGCCTGGCGGAGAAATACCGCTT
GCCGGTTGCCACTACGCTGTCCGGCAAGGGCGCTTTTCCCGAAGGGCACGAGCTGGCGCTGGGGCTGTATGGTTTCGCCG
GTCATACCCGGGCGGTGGAAACCATCAATGGCGACGAGGTCGACGTGCTGCTGGTGCTGGGCTGCGATCTCAGCCAGCGC
GACAGCCTGAACTGGAATTCACGGCTGCATGGCACCAAAACGCTGGTGGTGATTGATGAAGATTTCGACAAGGTCAGCTC
GCATTATCAGCCGGACATGCAGATTTTCTCCAGCCTGACCGATACGCTGCGGCACCTGCTGGATGCGGTCAGCGATGAAG
ATCAGCATCTGGTCGCCATCAAGAGCCTGCGCAATGGCTGGCTTGAACAGGTGCGCCAACTGCCGTTGGAGCAACAGCAG
CCCGATTTACAGGCGCGCCTGGCCCTGGGTGAAGCCAATATGTACCCCGGCGACGCCATTAAACGCATTCGCAGCCGGAT
GGGCCCGGACACCAACGTAGTGGTCGACTCCGGCGCGCACCGCATCTTTATGGCGCACCATTGGCTGGCCAGCGGGCGTG
GTGATTACCATACCTCAAGTTCCCTGGCACCGATGGGCTGGGCTATTTGTGCCGGTATTGGCATCAAGCTTGCGGCACCG
CACCGCGACTGCGTGGTGGTCACCGGCGACGGATGCATGTTGATGCACGGTATGGAGATCCAGACCGCCGCCCGTTATCA
GGTCAAAATGACCTTTGTGGTGATGAACAACTGCGCCCACGGGGCGATGCATATCGACACCTTGCAGAACCGCGGCGTCT
CGGCGGACTACACGGCGTTGCCAAACCACGACTGGAAGGCCTTCGCCAACAGTTTGGGAGTTTCTTCTGCCAGGGCGGCG
ACGCTGGAAGAGCTGGATGAGGCACTGGTCGCGGCGGCTGAGCACGAAGGCCCGTTCCTGATAGAAGTGTTGGTAGGAAA
TCATGTGGCACCTAATCGCTACTATGCCGAGAGCATCGTAGAATACGAGCAGCGTATCAAAGGCTTGTAA

Upstream 100 bases:

>100_bases
AACGCGAAAGCAGATGCGCATGAGAGCCTGTCTCTGTTGGCTAACAGCCTGGTGGGATCGACTCTGAATCAGGTTGGCCA
ACCACGACTGGACATTAAAA

Downstream 100 bases:

>100_bases
CGACGTAGCCCTGTTATTGCGGGAGTCGTCATTATTGTCCTTTGTATCACGCCATTCTATTTTACTTACAGTAGTCATGG
AGCGATAACGATGTATAAAC

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MESHAEKLYTPVALATADGVRVIATPGTKAAEQTNADVIIQGLEARGIQHIFLVPGKLVYPLIKSIEHSAISGIVGAHET
ACGFMADGYARASRKFGVCLGISGPGTMNFLPAMAAAQADKIPVLYLAGGIATYHEAQGAFQDGSNSGIDELTIVKPLLS
AAIEVKNNLTLQHELRRSLSCLNTQRKGRAYLSVPVDMQKKTVAGNYEASPQRTPQCREAAIDQQALEQVLDDYLLRGLN
VACLVGNRMNNPLDAALLLRLAEKYRLPVATTLSGKGAFPEGHELALGLYGFAGHTRAVETINGDEVDVLLVLGCDLSQR
DSLNWNSRLHGTKTLVVIDEDFDKVSSHYQPDMQIFSSLTDTLRHLLDAVSDEDQHLVAIKSLRNGWLEQVRQLPLEQQQ
PDLQARLALGEANMYPGDAIKRIRSRMGPDTNVVVDSGAHRIFMAHHWLASGRGDYHTSSSLAPMGWAICAGIGIKLAAP
HRDCVVVTGDGCMLMHGMEIQTAARYQVKMTFVVMNNCAHGAMHIDTLQNRGVSADYTALPNHDWKAFANSLGVSSARAA
TLEELDEALVAAAEHEGPFLIEVLVGNHVAPNRYYAESIVEYEQRIKGL

Sequences:

>Translated_609_residues
MESHAEKLYTPVALATADGVRVIATPGTKAAEQTNADVIIQGLEARGIQHIFLVPGKLVYPLIKSIEHSAISGIVGAHET
ACGFMADGYARASRKFGVCLGISGPGTMNFLPAMAAAQADKIPVLYLAGGIATYHEAQGAFQDGSNSGIDELTIVKPLLS
AAIEVKNNLTLQHELRRSLSCLNTQRKGRAYLSVPVDMQKKTVAGNYEASPQRTPQCREAAIDQQALEQVLDDYLLRGLN
VACLVGNRMNNPLDAALLLRLAEKYRLPVATTLSGKGAFPEGHELALGLYGFAGHTRAVETINGDEVDVLLVLGCDLSQR
DSLNWNSRLHGTKTLVVIDEDFDKVSSHYQPDMQIFSSLTDTLRHLLDAVSDEDQHLVAIKSLRNGWLEQVRQLPLEQQQ
PDLQARLALGEANMYPGDAIKRIRSRMGPDTNVVVDSGAHRIFMAHHWLASGRGDYHTSSSLAPMGWAICAGIGIKLAAP
HRDCVVVTGDGCMLMHGMEIQTAARYQVKMTFVVMNNCAHGAMHIDTLQNRGVSADYTALPNHDWKAFANSLGVSSARAA
TLEELDEALVAAAEHEGPFLIEVLVGNHVAPNRYYAESIVEYEQRIKGL
>Mature_609_residues
MESHAEKLYTPVALATADGVRVIATPGTKAAEQTNADVIIQGLEARGIQHIFLVPGKLVYPLIKSIEHSAISGIVGAHET
ACGFMADGYARASRKFGVCLGISGPGTMNFLPAMAAAQADKIPVLYLAGGIATYHEAQGAFQDGSNSGIDELTIVKPLLS
AAIEVKNNLTLQHELRRSLSCLNTQRKGRAYLSVPVDMQKKTVAGNYEASPQRTPQCREAAIDQQALEQVLDDYLLRGLN
VACLVGNRMNNPLDAALLLRLAEKYRLPVATTLSGKGAFPEGHELALGLYGFAGHTRAVETINGDEVDVLLVLGCDLSQR
DSLNWNSRLHGTKTLVVIDEDFDKVSSHYQPDMQIFSSLTDTLRHLLDAVSDEDQHLVAIKSLRNGWLEQVRQLPLEQQQ
PDLQARLALGEANMYPGDAIKRIRSRMGPDTNVVVDSGAHRIFMAHHWLASGRGDYHTSSSLAPMGWAICAGIGIKLAAP
HRDCVVVTGDGCMLMHGMEIQTAARYQVKMTFVVMNNCAHGAMHIDTLQNRGVSADYTALPNHDWKAFANSLGVSSARAA
TLEELDEALVAAAEHEGPFLIEVLVGNHVAPNRYYAESIVEYEQRIKGL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=589, Percent_Identity=22.0713073005093, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI21361361, Length=604, Percent_Identity=21.6887417218543, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1790104, Length=505, Percent_Identity=26.7326732673267, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI87081685, Length=488, Percent_Identity=26.0245901639344, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1786717, Length=586, Percent_Identity=24.7440273037543, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1787096, Length=564, Percent_Identity=22.6950354609929, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788716, Length=569, Percent_Identity=20.2108963093146, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17542570, Length=598, Percent_Identity=19.8996655518395, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=21.4411247803163, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=21.4411247803163, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=23.8500851788756, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320816, Length=501, Percent_Identity=21.3572854291417, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=576, Percent_Identity=22.0486111111111, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 66051; Mature: 66051

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESHAEKLYTPVALATADGVRVIATPGTKAAEQTNADVIIQGLEARGIQHIFLVPGKLVY
CCCCHHHHHCCEEEEECCCEEEEECCCCCHHHHCCCEEEEEEHHHCCCEEEEECCCHHHH
PLIKSIEHSAISGIVGAHETACGFMADGYARASRKFGVCLGISGPGTMNFLPAMAAAQAD
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCC
KIPVLYLAGGIATYHEAQGAFQDGSNSGIDELTIVKPLLSAAIEVKNNLTLQHELRRSLS
CCCEEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
CLNTQRKGRAYLSVPVDMQKKTVAGNYEASPQRTPQCREAAIDQQALEQVLDDYLLRGLN
HHCHHCCCCEEEEECCCCCCCEECCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCC
VACLVGNRMNNPLDAALLLRLAEKYRLPVATTLSGKGAFPEGHELALGLYGFAGHTRAVE
EEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCEEE
TINGDEVDVLLVLGCDLSQRDSLNWNSRLHGTKTLVVIDEDFDKVSSHYQPDMQIFSSLT
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHHHHH
DTLRHLLDAVSDEDQHLVAIKSLRNGWLEQVRQLPLEQQQPDLQARLALGEANMYPGDAI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCCHHH
KRIRSRMGPDTNVVVDSGAHRIFMAHHWLASGRGDYHTSSSLAPMGWAICAGIGIKLAAP
HHHHHHCCCCCEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECC
HRDCVVVTGDGCMLMHGMEIQTAARYQVKMTFVVMNNCAHGAMHIDTLQNRGVSADYTAL
CCCEEEEECCCEEEEECCEEECCCEEEEEEEEEEEECCCCCCEEEHHHHCCCCCCCCCCC
PNHDWKAFANSLGVSSARAATLEELDEALVAAAEHEGPFLIEVLVGNHVAPNRYYAESIV
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
EYEQRIKGL
HHHHHHCCC
>Mature Secondary Structure
MESHAEKLYTPVALATADGVRVIATPGTKAAEQTNADVIIQGLEARGIQHIFLVPGKLVY
CCCCHHHHHCCEEEEECCCEEEEECCCCCHHHHCCCEEEEEEHHHCCCEEEEECCCHHHH
PLIKSIEHSAISGIVGAHETACGFMADGYARASRKFGVCLGISGPGTMNFLPAMAAAQAD
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCC
KIPVLYLAGGIATYHEAQGAFQDGSNSGIDELTIVKPLLSAAIEVKNNLTLQHELRRSLS
CCCEEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
CLNTQRKGRAYLSVPVDMQKKTVAGNYEASPQRTPQCREAAIDQQALEQVLDDYLLRGLN
HHCHHCCCCEEEEECCCCCCCEECCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCC
VACLVGNRMNNPLDAALLLRLAEKYRLPVATTLSGKGAFPEGHELALGLYGFAGHTRAVE
EEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCEEE
TINGDEVDVLLVLGCDLSQRDSLNWNSRLHGTKTLVVIDEDFDKVSSHYQPDMQIFSSLT
ECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHHHHH
DTLRHLLDAVSDEDQHLVAIKSLRNGWLEQVRQLPLEQQQPDLQARLALGEANMYPGDAI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECCCCCCCCHHH
KRIRSRMGPDTNVVVDSGAHRIFMAHHWLASGRGDYHTSSSLAPMGWAICAGIGIKLAAP
HHHHHHCCCCCEEEEECCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEECC
HRDCVVVTGDGCMLMHGMEIQTAARYQVKMTFVVMNNCAHGAMHIDTLQNRGVSADYTAL
CCCEEEEECCCEEEEECCEEECCCEEEEEEEEEEEECCCCCCEEEHHHHCCCCCCCCCCC
PNHDWKAFANSLGVSSARAATLEELDEALVAAAEHEGPFLIEVLVGNHVAPNRYYAESIV
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHH
EYEQRIKGL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]