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The map label for this gene is ynfL [H]

Identifier: 157371428

GI number: 157371428

Start: 3512105

End: 3512998

Strand: Reverse

Name: ynfL [H]

Synonym: Spro_3191

Alternate gene names: 157371428

Gene position: 3512998-3512105 (Counterclockwise)

Preceding gene: 157371430

Following gene: 157371427

Centisome position: 64.47

GC content: 45.75

Gene sequence:

>894_bases
ATGCTTGGTAATATTCATAACGTTGATATCCGATTGTTACAGATATTTTTGACCATAGTGCAGTGTAATGGATTCTCGGC
CGCTCAGGCACAGCTGAATATGAGCCAGTCCGCCATCAGTACATGTATGTCGACTCTGGAGACGCGGCTGGGCTTTCGCC
TCTGTGAACGGGGTAAAAAAGGATTCAGTCTGACAGCGGAAGGGGAACAGGTGCTGTGTTATACACAACAACTGTTCGCC
AAATTAGATGCTTTTGTTTTACAGGTTCATAACCTTTCAGGGCGGCTACGCGGCGAGTTAATGATCGGGCTGTTAGACAG
CACATTGACGTTGCCCGAAGCAAAAGTAGTTGAGGCAATAAGACGTTTTTATCAGCGTGATCATGAGGTAAGTCTGCAGG
TTTTTATTAAATCACCAACGGAACTTGAACAAGAAATTATCAGCGGCGAACTGCATGCGGCGATATCTTATATTGGACAC
AGATTAGAAAACCTTGAGTATATCGATCTCTTCAGTGAAAAAATTTCAATCTATTGCGGAAAGCACCATCCGCTGTATTT
ATCATCAAAGGTAACCTCCGAGGACTTACTGCGCTTCAGTTGGGTGAAGCGTGGCTATCTGATGCCGTCGGATTTGGTGC
CTGTAATGCCGCCACTAATCACTGCCACGGCCCACCAGATGGAGGCTGTTGCCTTACTGGTTTTGGCAGGGACACACATT
GGTTATCTGCCGCAACATTATGCTCAGCAGTGGGTTGATAAACAGGAAATGTACTTGCTGAAATCTGAAGAATTAAGCTA
TGAGGTGACTCACTGCCTTATTCTTAACCGAAGTCGCCCGCATAATGAAGCACTCGAAGCCCTAGTGTCTGACATTCTTC
TGGAACATGGATGA

Upstream 100 bases:

>100_bases
AAACATCGAAACCAGGGATTTTGCTTTCAGCCAATTCTGGAACCCAATAAAGAGGAGTTTGAATATTGAGTTAATTATAC
TCATTATATAAGGAGGGGTC

Downstream 100 bases:

>100_bases
CCCTACTAAAAATAATGCATACTTATATTACAACGCTTTCCGGTTAAACATTCATAAATTATAAAGTCAGCTTTGTTTTA
TATCTATTGTTAGCAATGTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLGNIHNVDIRLLQIFLTIVQCNGFSAAQAQLNMSQSAISTCMSTLETRLGFRLCERGKKGFSLTAEGEQVLCYTQQLFA
KLDAFVLQVHNLSGRLRGELMIGLLDSTLTLPEAKVVEAIRRFYQRDHEVSLQVFIKSPTELEQEIISGELHAAISYIGH
RLENLEYIDLFSEKISIYCGKHHPLYLSSKVTSEDLLRFSWVKRGYLMPSDLVPVMPPLITATAHQMEAVALLVLAGTHI
GYLPQHYAQQWVDKQEMYLLKSEELSYEVTHCLILNRSRPHNEALEALVSDILLEHG

Sequences:

>Translated_297_residues
MLGNIHNVDIRLLQIFLTIVQCNGFSAAQAQLNMSQSAISTCMSTLETRLGFRLCERGKKGFSLTAEGEQVLCYTQQLFA
KLDAFVLQVHNLSGRLRGELMIGLLDSTLTLPEAKVVEAIRRFYQRDHEVSLQVFIKSPTELEQEIISGELHAAISYIGH
RLENLEYIDLFSEKISIYCGKHHPLYLSSKVTSEDLLRFSWVKRGYLMPSDLVPVMPPLITATAHQMEAVALLVLAGTHI
GYLPQHYAQQWVDKQEMYLLKSEELSYEVTHCLILNRSRPHNEALEALVSDILLEHG
>Mature_297_residues
MLGNIHNVDIRLLQIFLTIVQCNGFSAAQAQLNMSQSAISTCMSTLETRLGFRLCERGKKGFSLTAEGEQVLCYTQQLFA
KLDAFVLQVHNLSGRLRGELMIGLLDSTLTLPEAKVVEAIRRFYQRDHEVSLQVFIKSPTELEQEIISGELHAAISYIGH
RLENLEYIDLFSEKISIYCGKHHPLYLSSKVTSEDLLRFSWVKRGYLMPSDLVPVMPPLITATAHQMEAVALLVLAGTHI
GYLPQHYAQQWVDKQEMYLLKSEELSYEVTHCLILNRSRPHNEALEALVSDILLEHG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=273, Percent_Identity=25.2747252747253, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787949, Length=279, Percent_Identity=25.4480286738351, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI157672245, Length=182, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=27.8606965174129, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=300, Percent_Identity=23, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788887, Length=217, Percent_Identity=27.1889400921659, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33626; Mature: 33626

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGNIHNVDIRLLQIFLTIVQCNGFSAAQAQLNMSQSAISTCMSTLETRLGFRLCERGKK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFSLTAEGEQVLCYTQQLFAKLDAFVLQVHNLSGRLRGELMIGLLDSTLTLPEAKVVEAI
CCEEECCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEHHHEEEECCCCCCCCHHHHHHHH
RRFYQRDHEVSLQVFIKSPTELEQEIISGELHAAISYIGHRLENLEYIDLFSEKISIYCG
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEC
KHHPLYLSSKVTSEDLLRFSWVKRGYLMPSDLVPVMPPLITATAHQMEAVALLVLAGTHI
CCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCHH
GYLPQHYAQQWVDKQEMYLLKSEELSYEVTHCLILNRSRPHNEALEALVSDILLEHG
CCCHHHHHHHHCCHHHHEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLGNIHNVDIRLLQIFLTIVQCNGFSAAQAQLNMSQSAISTCMSTLETRLGFRLCERGKK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFSLTAEGEQVLCYTQQLFAKLDAFVLQVHNLSGRLRGELMIGLLDSTLTLPEAKVVEAI
CCEEECCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEHHHEEEECCCCCCCCHHHHHHHH
RRFYQRDHEVSLQVFIKSPTELEQEIISGELHAAISYIGHRLENLEYIDLFSEKISIYCG
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEC
KHHPLYLSSKVTSEDLLRFSWVKRGYLMPSDLVPVMPPLITATAHQMEAVALLVLAGTHI
CCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCHH
GYLPQHYAQQWVDKQEMYLLKSEELSYEVTHCLILNRSRPHNEALEALVSDILLEHG
CCCHHHHHHHHCCHHHHEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]