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The map label for this gene is entD [H]

Identifier: 157371402

GI number: 157371402

Start: 3481223

End: 3481918

Strand: Reverse

Name: entD [H]

Synonym: Spro_3163

Alternate gene names: 157371402

Gene position: 3481918-3481223 (Counterclockwise)

Preceding gene: 157371403

Following gene: 157371400

Centisome position: 63.9

GC content: 57.04

Gene sequence:

>696_bases
GTGTTCTCTTCTTTTATCCCCAGCATTGAATGGCTGAACCTGGATGATTACCCAGGACGGGTTGCCCGTTGCCGCTTTTC
TTTGGCGAACTATCAGGATGGCTGTTTCGACGCCATTGGCATGAGCATCCCCGAACATCTGGCCCGTGCCGTGCCGAAGC
GGCGGGCGGAGTATCTGGCCGGGCGTTACCTGGCGCGGGAGTTGCTGGCTACGCTGGGGCTTGCTGATTTCACGTTGTTG
CGCGGGGAAGACCGTGCGCCGCAGTGGCCGCAGGGCATCGCAGGGGCAATCAGCCATAATGCGGATACCGCGCTCTGCGC
CGTACACCGGGAGAGCGGGCTGGGTGGCGTGGGGCTGGACGTTGAAACGTTAATCCCCACGGCACGGGCGGAGGAGCTAT
GGGGGGCTATCATCAGCGAGACGGAATATGAATACCTGCGCGCCCAACCGCAGGCATTCAACTGGCTGCTGACGCTAGTG
TTCTCCGCCAAAGAAAGCCTGTTCAAAGCCTTGTACACACAGGTACGTAGCTATTTTGATTTTCTCGATGCACGCCTGGT
GGCGGTTGATATACAGCAACAAACCTTTGCATTCGAACTGCTAAAACATCTCACGCCGCATTGCCACGCTGGCCGCCGTT
TTGGCGGTCGTTATTGGCAACAAGGTGACGATGTCACAACTTTTATCTGTTTATAA

Upstream 100 bases:

>100_bases
CGCCATTCCTCTTCCCCGGTTGCGGGGGAGAGGAATAATCATTGTTGCCCATGGGTTCGGCGTCTAAAATTACCGTTTAC
ACACCTAACATGGATGACAA

Downstream 100 bases:

>100_bases
TCAATGAAATGAAAAAGTTGCGCTGTCACATTAATCACAAGGCCCGTCGCCGTCGAACCAGGTGACGGGCGCCCTCCGAT
ACCAGTAACAGCACCGCCAG

Product: 4'-phosphopantetheinyl transferase

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MFSSFIPSIEWLNLDDYPGRVARCRFSLANYQDGCFDAIGMSIPEHLARAVPKRRAEYLAGRYLARELLATLGLADFTLL
RGEDRAPQWPQGIAGAISHNADTALCAVHRESGLGGVGLDVETLIPTARAEELWGAIISETEYEYLRAQPQAFNWLLTLV
FSAKESLFKALYTQVRSYFDFLDARLVAVDIQQQTFAFELLKHLTPHCHAGRRFGGRYWQQGDDVTTFICL

Sequences:

>Translated_231_residues
MFSSFIPSIEWLNLDDYPGRVARCRFSLANYQDGCFDAIGMSIPEHLARAVPKRRAEYLAGRYLARELLATLGLADFTLL
RGEDRAPQWPQGIAGAISHNADTALCAVHRESGLGGVGLDVETLIPTARAEELWGAIISETEYEYLRAQPQAFNWLLTLV
FSAKESLFKALYTQVRSYFDFLDARLVAVDIQQQTFAFELLKHLTPHCHAGRRFGGRYWQQGDDVTTFICL
>Mature_231_residues
MFSSFIPSIEWLNLDDYPGRVARCRFSLANYQDGCFDAIGMSIPEHLARAVPKRRAEYLAGRYLARELLATLGLADFTLL
RGEDRAPQWPQGIAGAISHNADTALCAVHRESGLGGVGLDVETLIPTARAEELWGAIISETEYEYLRAQPQAFNWLLTLV
FSAKESLFKALYTQVRSYFDFLDARLVAVDIQQQTFAFELLKHLTPHCHAGRRFGGRYWQQGDDVTTFICL

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=184, Percent_Identity=33.1521739130435, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 26025; Mature: 26025

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSFIPSIEWLNLDDYPGRVARCRFSLANYQDGCFDAIGMSIPEHLARAVPKRRAEYLA
CCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
GRYLARELLATLGLADFTLLRGEDRAPQWPQGIAGAISHNADTALCAVHRESGLGGVGLD
HHHHHHHHHHHHCHHHHHEECCCCCCCCCCCHHHHHHCCCCCHHHEEEEHHCCCCCCCCC
VETLIPTARAEELWGAIISETEYEYLRAQPQAFNWLLTLVFSAKESLFKALYTQVRSYFD
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLDARLVAVDIQQQTFAFELLKHLTPHCHAGRRFGGRYWQQGDDVTTFICL
HHHHHHHEEEHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCCCCCEEEEEEC
>Mature Secondary Structure
MFSSFIPSIEWLNLDDYPGRVARCRFSLANYQDGCFDAIGMSIPEHLARAVPKRRAEYLA
CCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
GRYLARELLATLGLADFTLLRGEDRAPQWPQGIAGAISHNADTALCAVHRESGLGGVGLD
HHHHHHHHHHHHCHHHHHEECCCCCCCCCCCHHHHHHCCCCCHHHEEEEHHCCCCCCCCC
VETLIPTARAEELWGAIISETEYEYLRAQPQAFNWLLTLVFSAKESLFKALYTQVRSYFD
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLDARLVAVDIQQQTFAFELLKHLTPHCHAGRRFGGRYWQQGDDVTTFICL
HHHHHHHEEEHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]