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The map label for this gene is alkB [H]

Identifier: 157371400

GI number: 157371400

Start: 3479516

End: 3480163

Strand: Reverse

Name: alkB [H]

Synonym: Spro_3161

Alternate gene names: 157371400

Gene position: 3480163-3479516 (Counterclockwise)

Preceding gene: 157371402

Following gene: 157371398

Centisome position: 63.87

GC content: 62.96

Gene sequence:

>648_bases
ATGACCCTCGATCTTTTTGAAGACTCCGTACCGCCGCCGTGGCGGGAACAGATTGCTCCCGGCGCGGTAGTGATGCACGG
TTTTGTGCGCGATCACGGTCCGGAGTTGCTGGCGGCAGTGAATGGGGTGATCGCTCAGGTGCCGTGGCGGCATATGACCA
CGCCGGGCGGTCACGTGATGTCGGTGGCGATGAGCTGGTGCGGCAACGGCTGGAGCAGCGACAGCCGCGGCTACCGTTAC
TCAGAGCGCGACTCGCGCAGCGGCAAACGCTGGCCGCCGATCCCGGCTATCCTGATGGCGCTGGCAGATGAGGCGGCGCA
GCAGGCGGGTTTTGCCCCTTTCGTGCCGGATTCCTGTCTGATGAACCGCTACGATCCGGGCAGCAAGCTCTCGTTGCATC
AGGATAAGGACGAGCATGATTTCGGCTCGCCGATCGTTTCCGTCTCACTGGGTCTGCCGGCGGTGTTCCAGTTCGGCGGC
ATGCAACGCAGCGATCGCGCCCAGCGCATACCCTTGGCGCACGGCGATGTGGTGGTGTGGGGCGGCCCGTCGCGTCTGTG
CTTCCACGGGATTATGCCAATTAAAGAGGGTTACCATTCGCTGGTGGGGCCACACCGCATTAACATCACGCTACGTAAGG
CGCTTTAG

Upstream 100 bases:

>100_bases
CTAGTGAAGTTTGGTGCTGACAACAATGCCGCGTATCACACTGGACAATCGGCAGTGGCGAGTTAAAATAAATACTGTGT
ATTAATACAGGTGTTATGCG

Downstream 100 bases:

>100_bases
TTGCGGCTCTTTTGCCAAACAGGCAACCAGATAGTCGATAAACACCCGCAGCTTGGGCGGCAAGTGCTGGTTGGGGCCAT
ACAGTAGCCACAGGCCGCCG

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTLDLFEDSVPPPWREQIAPGAVVMHGFVRDHGPELLAAVNGVIAQVPWRHMTTPGGHVMSVAMSWCGNGWSSDSRGYRY
SERDSRSGKRWPPIPAILMALADEAAQQAGFAPFVPDSCLMNRYDPGSKLSLHQDKDEHDFGSPIVSVSLGLPAVFQFGG
MQRSDRAQRIPLAHGDVVVWGGPSRLCFHGIMPIKEGYHSLVGPHRINITLRKAL

Sequences:

>Translated_215_residues
MTLDLFEDSVPPPWREQIAPGAVVMHGFVRDHGPELLAAVNGVIAQVPWRHMTTPGGHVMSVAMSWCGNGWSSDSRGYRY
SERDSRSGKRWPPIPAILMALADEAAQQAGFAPFVPDSCLMNRYDPGSKLSLHQDKDEHDFGSPIVSVSLGLPAVFQFGG
MQRSDRAQRIPLAHGDVVVWGGPSRLCFHGIMPIKEGYHSLVGPHRINITLRKAL
>Mature_214_residues
TLDLFEDSVPPPWREQIAPGAVVMHGFVRDHGPELLAAVNGVIAQVPWRHMTTPGGHVMSVAMSWCGNGWSSDSRGYRYS
ERDSRSGKRWPPIPAILMALADEAAQQAGFAPFVPDSCLMNRYDPGSKLSLHQDKDEHDFGSPIVSVSLGLPAVFQFGGM
QRSDRAQRIPLAHGDVVVWGGPSRLCFHGIMPIKEGYHSLVGPHRINITLRKAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=135, Percent_Identity=31.8518518518519, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1788541, Length=213, Percent_Identity=53.0516431924883, Blast_Score=230, Evalue=5e-62,
Organism=Drosophila melanogaster, GI45555401, Length=129, Percent_Identity=34.1085271317829, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23600; Mature: 23469

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDLFEDSVPPPWREQIAPGAVVMHGFVRDHGPELLAAVNGVIAQVPWRHMTTPGGHVM
CCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHCCCCCHHHH
SVAMSWCGNGWSSDSRGYRYSERDSRSGKRWPPIPAILMALADEAAQQAGFAPFVPDSCL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
MNRYDPGSKLSLHQDKDEHDFGSPIVSVSLGLPAVFQFGGMQRSDRAQRIPLAHGDVVVW
HCCCCCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCHHHCCCCCCCCEEEE
GGPSRLCFHGIMPIKEGYHSLVGPHRINITLRKAL
CCCCHHHHHCCCCHHHHHHHHCCCEEEEEEEEECC
>Mature Secondary Structure 
TLDLFEDSVPPPWREQIAPGAVVMHGFVRDHGPELLAAVNGVIAQVPWRHMTTPGGHVM
CCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHCCCCCHHHH
SVAMSWCGNGWSSDSRGYRYSERDSRSGKRWPPIPAILMALADEAAQQAGFAPFVPDSCL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHH
MNRYDPGSKLSLHQDKDEHDFGSPIVSVSLGLPAVFQFGGMQRSDRAQRIPLAHGDVVVW
HCCCCCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCHHHCCCCCCCCEEEE
GGPSRLCFHGIMPIKEGYHSLVGPHRINITLRKAL
CCCCHHHHHCCCCHHHHHHHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855 [H]