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The map label for this gene is yafC [C]

Identifier: 157371376

GI number: 157371376

Start: 3452338

End: 3453309

Strand: Reverse

Name: yafC [C]

Synonym: Spro_3137

Alternate gene names: 157371376

Gene position: 3453309-3452338 (Counterclockwise)

Preceding gene: 157371378

Following gene: 157371375

Centisome position: 63.38

GC content: 52.98

Gene sequence:

>972_bases
ATGGAAAGCCTTGGTTCCCTCGATGTCTTCGTTCGTGTCGGTGAGTCGCGCAGCTTCACGACTACCGGGCAACAACTAGG
TATCTCAGCATCAGCAGTTAGCAAGGCTATCGCTCGTTTGGAGGAGCGGTTAGCTACGCGACTGTTCCATCGGTCGACTC
GAAACGTCACTTTGACTCCAGAAGGAGCTCTCTTTCTGGAGCGTTGCAGACGCATTCTGGGTGAGGTGGAGGCGGCAGAA
AATGAGCTTGCACAATCCAACCAGATGCCTCGCGGCAAGCTTCGCGTTAGCCTGCCTTCCGTCGGTCTACTGTTCATGCC
GCAATTCGCCGAATTTAAACGTCAGTACCCCGACATAGAACTGGATATCGACTGCTCTGATCGTCTGGTGGATGTAATCG
AGGAAGGATTTGATGCGGTGATCCGTACGGGAGAGCCAAGTGATTCCCGGTTGATGGCACGTAAGCTCGGAAGCTATCGT
CGTATCATGGTGGGTGCACCGAGGTACTTCGAACAATGTGGGGTGCCGAATACCCCCGAGGAACTAATGCAGCACCGCTG
TCTACGATACCGATTCCCCAGTAGTGGCAAGGTCGACCAGTGGCCCCTCAGCCGCGGAGGCCAAATACTCACTTTTGATC
TAGCCAGTTCAATGACGGCCAATACGCTTGAACCGCTGGTTTGCTTCGCAGAGTCAGGTCTTGGCATCGCGTGTATGCCA
GACATTGCCATCCGTCAGCAACTGGAAGTCGGCAGCTTGGTATCCGTACTGGATGGATTCAATCAAGACCACACCATCTT
ACGGGTGCTGTGGCCTTCAAGCCGGCATCTTTCACCCAAGCTGAGAGTCTTCGTTGATTACGTCACCACCGCTCTATTTC
CAGATACAAGTAAGAAAACTGTTATCAACGGTGCAAACGTGATCCCCCCAACGGTTGAACTCACAGCTCATAGCAGACTT
CATGTTCCATAA

Upstream 100 bases:

>100_bases
TTGCAGAGCGCTACCCGACAGTGTGCTCGTTGGCGAAAAGAAGAAAAATAGGCAATAATTCACAACATATATGACAATTG
AGTCATTAATAAGGAAGCTC

Downstream 100 bases:

>100_bases
TCCTTATGCTGCAGATCCCTTAAGTGTATTTATGTGCAAAGAAATATAAATCATATAAATTAAATCTTTCTGCGCCCTGC
TAAAGAGTTCTACATGTTGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MESLGSLDVFVRVGESRSFTTTGQQLGISASAVSKAIARLEERLATRLFHRSTRNVTLTPEGALFLERCRRILGEVEAAE
NELAQSNQMPRGKLRVSLPSVGLLFMPQFAEFKRQYPDIELDIDCSDRLVDVIEEGFDAVIRTGEPSDSRLMARKLGSYR
RIMVGAPRYFEQCGVPNTPEELMQHRCLRYRFPSSGKVDQWPLSRGGQILTFDLASSMTANTLEPLVCFAESGLGIACMP
DIAIRQQLEVGSLVSVLDGFNQDHTILRVLWPSSRHLSPKLRVFVDYVTTALFPDTSKKTVINGANVIPPTVELTAHSRL
HVP

Sequences:

>Translated_323_residues
MESLGSLDVFVRVGESRSFTTTGQQLGISASAVSKAIARLEERLATRLFHRSTRNVTLTPEGALFLERCRRILGEVEAAE
NELAQSNQMPRGKLRVSLPSVGLLFMPQFAEFKRQYPDIELDIDCSDRLVDVIEEGFDAVIRTGEPSDSRLMARKLGSYR
RIMVGAPRYFEQCGVPNTPEELMQHRCLRYRFPSSGKVDQWPLSRGGQILTFDLASSMTANTLEPLVCFAESGLGIACMP
DIAIRQQLEVGSLVSVLDGFNQDHTILRVLWPSSRHLSPKLRVFVDYVTTALFPDTSKKTVINGANVIPPTVELTAHSRL
HVP
>Mature_323_residues
MESLGSLDVFVRVGESRSFTTTGQQLGISASAVSKAIARLEERLATRLFHRSTRNVTLTPEGALFLERCRRILGEVEAAE
NELAQSNQMPRGKLRVSLPSVGLLFMPQFAEFKRQYPDIELDIDCSDRLVDVIEEGFDAVIRTGEPSDSRLMARKLGSYR
RIMVGAPRYFEQCGVPNTPEELMQHRCLRYRFPSSGKVDQWPLSRGGQILTFDLASSMTANTLEPLVCFAESGLGIACMP
DIAIRQQLEVGSLVSVLDGFNQDHTILRVLWPSSRHLSPKLRVFVDYVTTALFPDTSKKTVINGANVIPPTVELTAHSRL
HVP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=35.7638888888889, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=29.9319727891156, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787128, Length=298, Percent_Identity=30.5369127516779, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=29.5138888888889, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=28.8659793814433, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=31.9444444444444, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=29.0657439446367, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790011, Length=257, Percent_Identity=22.9571984435798, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=25.6849315068493, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789173, Length=253, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788706, Length=261, Percent_Identity=25.6704980842912, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788481, Length=301, Percent_Identity=26.9102990033223, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788748, Length=121, Percent_Identity=37.1900826446281, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=122, Percent_Identity=31.1475409836066, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=33.6134453781513, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=139, Percent_Identity=32.3741007194245, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35963; Mature: 35963

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLGSLDVFVRVGESRSFTTTGQQLGISASAVSKAIARLEERLATRLFHRSTRNVTLTP
CCCCCCEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
EGALFLERCRRILGEVEAAENELAQSNQMPRGKLRVSLPSVGLLFMPQFAEFKRQYPDIE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCCCHHHHHHHCCCEE
LDIDCSDRLVDVIEEGFDAVIRTGEPSDSRLMARKLGSYRRIMVGAPRYFEQCGVPNTPE
EECCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCHH
ELMQHRCLRYRFPSSGKVDQWPLSRGGQILTFDLASSMTANTLEPLVCFAESGLGIACMP
HHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEECC
DIAIRQQLEVGSLVSVLDGFNQDHTILRVLWPSSRHLSPKLRVFVDYVTTALFPDTSKKT
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCE
VINGANVIPPTVELTAHSRLHVP
EECCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MESLGSLDVFVRVGESRSFTTTGQQLGISASAVSKAIARLEERLATRLFHRSTRNVTLTP
CCCCCCEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
EGALFLERCRRILGEVEAAENELAQSNQMPRGKLRVSLPSVGLLFMPQFAEFKRQYPDIE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHCCCCHHHHHHHCCCEE
LDIDCSDRLVDVIEEGFDAVIRTGEPSDSRLMARKLGSYRRIMVGAPRYFEQCGVPNTPE
EECCCHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCHH
ELMQHRCLRYRFPSSGKVDQWPLSRGGQILTFDLASSMTANTLEPLVCFAESGLGIACMP
HHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEECC
DIAIRQQLEVGSLVSVLDGFNQDHTILRVLWPSSRHLSPKLRVFVDYVTTALFPDTSKKT
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCE
VINGANVIPPTVELTAHSRLHVP
EECCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]