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The map label for this gene is fosA [H]

Identifier: 157371375

GI number: 157371375

Start: 3451903

End: 3452244

Strand: Reverse

Name: fosA [H]

Synonym: Spro_3136

Alternate gene names: 157371375

Gene position: 3452244-3451903 (Counterclockwise)

Preceding gene: 157371376

Following gene: 157371369

Centisome position: 63.36

GC content: 44.15

Gene sequence:

>342_bases
ATGTTGAGCGGACTAAATCACATCACCCTTGCAGTGAGTAACGTAGCAAACAGTCTGAATTTTTATGTTGAGACGCTTGG
GTTCACACTTAAAGCCAAATGGGCTCATGGAGCTTACCTTTCGTTGGGTGAACTCTGGCTGTGTCTTTCAGGGGATAAAG
TATCTGTTGGTGATGACTACACGCATTATGCATTCAGTATTCCTACTGAAGAATTCGATGTGTTCGTTGAACGGTTGAGG
TCTTCTGGTGTTACAGAATGGAAAACTAACAAGAGCGAGGGGAAGTCGGTCTATTTTTTAGACCCCGATGGTCACAGGCT
TGAGATCCATGATGGTGAATAG

Upstream 100 bases:

>100_bases
TCCATAATCCTTATGCTGCAGATCCCTTAAGTGTATTTATGTGCAAAGAAATATAAATCATATAAATTAAATCTTTCTGC
GCCCTGCTAAAGAGTTCTAC

Downstream 100 bases:

>100_bases
ACCCGGATTTCCTAGACGGTTCCGTGCCTCATGAACCAGGCACTTTCATAAACTGCAGGGGGTTGATCCTCACAGGCACT
GTGGCGGCGTTTAGCATTAT

Product: glutathione transferase

Products: NA

Alternate protein names: Fosfomycin resistance protein [H]

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MLSGLNHITLAVSNVANSLNFYVETLGFTLKAKWAHGAYLSLGELWLCLSGDKVSVGDDYTHYAFSIPTEEFDVFVERLR
SSGVTEWKTNKSEGKSVYFLDPDGHRLEIHDGE

Sequences:

>Translated_113_residues
MLSGLNHITLAVSNVANSLNFYVETLGFTLKAKWAHGAYLSLGELWLCLSGDKVSVGDDYTHYAFSIPTEEFDVFVERLR
SSGVTEWKTNKSEGKSVYFLDPDGHRLEIHDGE
>Mature_113_residues
MLSGLNHITLAVSNVANSLNFYVETLGFTLKAKWAHGAYLSLGELWLCLSGDKVSVGDDYTHYAFSIPTEEFDVFVERLR
SSGVTEWKTNKSEGKSVYFLDPDGHRLEIHDGE

Specific function: Metalloglutathione transferase which confers resistance to fosfomycin by catalyzing the addition of glutathione to fosfomycin [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =2.5.1.18 [H]

Molecular weight: Translated: 12644; Mature: 12644

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGLNHITLAVSNVANSLNFYVETLGFTLKAKWAHGAYLSLGELWLCLSGDKVSVGDDY
CCCCCCCEEEEEHHHHHHHHHEEEECCEEEEEEECCCCEEEECEEEEEECCCEEECCCCC
THYAFSIPTEEFDVFVERLRSSGVTEWKTNKSEGKSVYFLDPDGHRLEIHDGE
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEECCC
>Mature Secondary Structure
MLSGLNHITLAVSNVANSLNFYVETLGFTLKAKWAHGAYLSLGELWLCLSGDKVSVGDDY
CCCCCCCEEEEEHHHHHHHHHEEEECCEEEEEEECCCCEEEECEEEEEECCCEEECCCCC
THYAFSIPTEEFDVFVERLRSSGVTEWKTNKSEGKSVYFLDPDGHRLEIHDGE
CEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1963292 [H]