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The map label for this gene is cytR [H]

Identifier: 157371353

GI number: 157371353

Start: 3427199

End: 3428251

Strand: Reverse

Name: cytR [H]

Synonym: Spro_3114

Alternate gene names: 157371353

Gene position: 3428251-3427199 (Counterclockwise)

Preceding gene: 157371358

Following gene: 157371350

Centisome position: 62.92

GC content: 59.92

Gene sequence:

>1053_bases
ATGGATATGAAGATGGCGGTGGACAAGGCGATGATCAGTGCGAAAGACGTGGCGGAATTGGCTGGAGTTTCCCGATCGGC
GGTCTCGCGGACTTATACGTCTGGTGCCAGCGTGTCGGCGGCGACCCGCGAGAAGGTGATGCGGGCGGCTGAAGAACTGG
GATATCACGTTAACCATTTGGCTCGGGGATTGGTACGTAACCGCAGCGGCATTGTTTGCCTGATCGTTTCAGAGTTGGCG
ACCCCTTACCGGGCCAGCCTGGTGCGCTTGCTGACCCAGCATTTGCAGGACGCGGGCAAAATAGCCATGTTGATTAACAC
CGATCGCTCCGATGACAGCGTCTCCCAGGCGCTGCAACAGGCAATAAACTTTCGGGCGGATGCCTCCTTTGTACTATCCG
GCATGCCCGACAGCGCCATTGCCCGCCTGTGTCACAGGCACGGCCAGCGGCTGGTGCTGATTAACCGTGATGACACCTTG
CCCGGCTCACTCAGCATCCGGCTGGATAACGAAGAAGCGGCGGGCAGAGTGGTGATGGCGTTCGCCCGTGCGGGCTGCCA
ACGCATCGCTTTTGCCAACTCTTTGGCCGGTACGCCCAGCCTGATGGCGCGTGAACGGGGTTTTCTGCAGGCAGCGGAAG
CGGCGGGGTTACCGGTGAGCGTCGAACGCTTCGGTACCACCTCCTATGAGAGCGGTCAGATCCTGGCGCACCGCCTGTTG
ACCCGGCGCGAGCGGCCTGATGCGGTGTTCTGCGTCACGGATTTATTGGCCTGCGGCTTTATGGACGAGGCGCGCCATCG
TTTTGGGCTGCGTATTCCTGAAGAACTTTGTGTTGCGGGCTACGACGATATTCCGCAGGCGGCCTGGTCTTCTTACCATC
TCACCACTTTTACCCAGCCGGTGGCGATATTTGCTCAGGATTCGGTTGCGTGGCTGGTGGCCGAAGAGAAGAAAGAAGAG
AGCATGATGCCGGCACTGGGGCAGGAGATGCCGGGTGAAACCCGGCTGTACCATGCTGATTTTGTCTGGCGTGGCACCAT
TCGCGGGGCCTGA

Upstream 100 bases:

>100_bases
AACAATTCAACCTTCTCGTCACAAAAATGAAACATTTTTACCGATAAAAATTTGCACAGCTTTGCAAAGTGCGGCAAACT
TTTCGCAGAGCAGAAAATGG

Downstream 100 bases:

>100_bases
GTTTCCTTCATTTCAGATTGTGCATGGCGCGTTGGCAGATCCGGCGGAACTTGCTAAGTTACATGGCATCTCAGGTCAAC
ACCATTTTCTATTAGCCGCG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDMKMAVDKAMISAKDVAELAGVSRSAVSRTYTSGASVSAATREKVMRAAEELGYHVNHLARGLVRNRSGIVCLIVSELA
TPYRASLVRLLTQHLQDAGKIAMLINTDRSDDSVSQALQQAINFRADASFVLSGMPDSAIARLCHRHGQRLVLINRDDTL
PGSLSIRLDNEEAAGRVVMAFARAGCQRIAFANSLAGTPSLMARERGFLQAAEAAGLPVSVERFGTTSYESGQILAHRLL
TRRERPDAVFCVTDLLACGFMDEARHRFGLRIPEELCVAGYDDIPQAAWSSYHLTTFTQPVAIFAQDSVAWLVAEEKKEE
SMMPALGQEMPGETRLYHADFVWRGTIRGA

Sequences:

>Translated_350_residues
MDMKMAVDKAMISAKDVAELAGVSRSAVSRTYTSGASVSAATREKVMRAAEELGYHVNHLARGLVRNRSGIVCLIVSELA
TPYRASLVRLLTQHLQDAGKIAMLINTDRSDDSVSQALQQAINFRADASFVLSGMPDSAIARLCHRHGQRLVLINRDDTL
PGSLSIRLDNEEAAGRVVMAFARAGCQRIAFANSLAGTPSLMARERGFLQAAEAAGLPVSVERFGTTSYESGQILAHRLL
TRRERPDAVFCVTDLLACGFMDEARHRFGLRIPEELCVAGYDDIPQAAWSSYHLTTFTQPVAIFAQDSVAWLVAEEKKEE
SMMPALGQEMPGETRLYHADFVWRGTIRGA
>Mature_350_residues
MDMKMAVDKAMISAKDVAELAGVSRSAVSRTYTSGASVSAATREKVMRAAEELGYHVNHLARGLVRNRSGIVCLIVSELA
TPYRASLVRLLTQHLQDAGKIAMLINTDRSDDSVSQALQQAINFRADASFVLSGMPDSAIARLCHRHGQRLVLINRDDTL
PGSLSIRLDNEEAAGRVVMAFARAGCQRIAFANSLAGTPSLMARERGFLQAAEAAGLPVSVERFGTTSYESGQILAHRLL
TRRERPDAVFCVTDLLACGFMDEARHRFGLRIPEELCVAGYDDIPQAAWSSYHLTTFTQPVAIFAQDSVAWLVAEEKKEE
SMMPALGQEMPGETRLYHADFVWRGTIRGA

Specific function: This protein negatively controls the transcription initiation of genes such as deoCABD, udp, and cdd encoding catabolizing enzymes and nupC, nupG, and tsx encoding transporting and pore-forming proteins. Binds cytidine and adenosine as effectors [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=353, Percent_Identity=26.9121813031161, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1789202, Length=300, Percent_Identity=30, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=30.5647840531561, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789068, Length=310, Percent_Identity=27.741935483871, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790194, Length=294, Percent_Identity=29.2517006802721, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787906, Length=252, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787948, Length=294, Percent_Identity=26.530612244898, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994940, Length=270, Percent_Identity=27.037037037037, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787580, Length=278, Percent_Identity=25.5395683453237, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786540, Length=303, Percent_Identity=28.7128712871287, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38258; Mature: 38258

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMKMAVDKAMISAKDVAELAGVSRSAVSRTYTSGASVSAATREKVMRAAEELGYHVNHL
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHH
ARGLVRNRSGIVCLIVSELATPYRASLVRLLTQHLQDAGKIAMLINTDRSDDSVSQALQQ
HHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
AINFRADASFVLSGMPDSAIARLCHRHGQRLVLINRDDTLPGSLSIRLDNEEAAGRVVMA
HHCCCCCCHHEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHH
FARAGCQRIAFANSLAGTPSLMARERGFLQAAEAAGLPVSVERFGTTSYESGQILAHRLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHHHH
TRRERPDAVFCVTDLLACGFMDEARHRFGLRIPEELCVAGYDDIPQAAWSSYHLTTFTQP
HHCCCCCEEHHHHHHHHHCCHHHHHHHCCCCCCHHHHHCCCCCCCHHHHCCEEEEECCCC
VAIFAQDSVAWLVAEEKKEESMMPALGQEMPGETRLYHADFVWRGTIRGA
EEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECEEEECCCCCC
>Mature Secondary Structure
MDMKMAVDKAMISAKDVAELAGVSRSAVSRTYTSGASVSAATREKVMRAAEELGYHVNHL
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHH
ARGLVRNRSGIVCLIVSELATPYRASLVRLLTQHLQDAGKIAMLINTDRSDDSVSQALQQ
HHHHHCCCCCEEEEEHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
AINFRADASFVLSGMPDSAIARLCHRHGQRLVLINRDDTLPGSLSIRLDNEEAAGRVVMA
HHCCCCCCHHEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHH
FARAGCQRIAFANSLAGTPSLMARERGFLQAAEAAGLPVSVERFGTTSYESGQILAHRLL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHHHH
TRRERPDAVFCVTDLLACGFMDEARHRFGLRIPEELCVAGYDDIPQAAWSSYHLTTFTQP
HHCCCCCEEHHHHHHHHHCCHHHHHHHCCCCCCHHHHHCCCCCCCHHHHCCEEEEECCCC
VAIFAQDSVAWLVAEEKKEESMMPALGQEMPGETRLYHADFVWRGTIRGA
EEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEECEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]