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The map label for this gene is mcl2 [H]

Identifier: 157371267

GI number: 157371267

Start: 3330475

End: 3331338

Strand: Reverse

Name: mcl2 [H]

Synonym: Spro_3027

Alternate gene names: 157371267

Gene position: 3331338-3330475 (Counterclockwise)

Preceding gene: 157371268

Following gene: 157371266

Centisome position: 61.14

GC content: 59.26

Gene sequence:

>864_bases
ATGACGGATAACACACGCGCCATTCCGCGCAGCATTCTGTATACGCCCGCCTTGTCGCTCAACCGGGTAGTCAAGGCGCT
GACTTATGACGCCGATGTCCACCTGATCGATCTGGAAGACTCGGTTCCCGGCCCGTCGAAGGCAGAAGGGCGGATTATCT
GCCGCACCGCTCTTGATAATGCCCCTCCGTCGGCGAATATTGCCGTGCGAATCAACGCGCTGGGTACGGTGGATGCGCTG
CATGATCTGGTGATGCTCTGTGGTGGCGGCACCGCGCCGGGGTTTATTGTCATGTCGATGGTCAGGTCACCTGCCGAGGT
GGTTTTTCTGCGTGAGACTTTTGCCTCTGCGGGCAAGCACCCGGAGATTTACCTGACGATAGAGACCGTTGAAGCCATTA
CCGATCTCGATGACGTCGCGGCCGTAGCCGATGGCCTGATTTTTGGCTCTGCGGATCTGGCGGCCACGCTGGGCGTACCC
ATCACCTGGTCGGGAATGCTGGCGGCACGTCAGGCGATGGTTCTGGCCAGTGCCAGACACGGCATTGGTTGCATTGATAC
CGCCAACTATCGTCTTTCTGAACCCGAGGTGCTGGCTCACGAAATCCAGAACATCAGGGATCTGGGCTTCCACGGTAAAG
CTACCGTGCACCCCAGTGAACTTGAGCCGATCAATTCAGCGTTACGGCCTAACGAAGGCGCGTTATTGGCGGCGCGCCGC
ATCACCGAGGCGGTCAAGGCTGCGCAAGGGGGCATTGCCTTGCTCGATGGTCATATGGTGGGGCCACCCTTCGCCCGCAT
GGCCCATACCACCCTGGGATTAGGCCAGGCCTGGGCTGCTCGTTTTAACAGGAGCAACTCATGA

Upstream 100 bases:

>100_bases
CCAAAGGCACTGCCGGTCTGCGGGTCATGATGCGTGCCGGACAAACAGAAGATCAAATTCGCCGTTTGTGCAAAATACTG
GCTGAAGTAAGGGCTCCCCA

Downstream 100 bases:

>100_bases
CGCTCTCCGTTGCCGTGATAGGGACCGGCGCCATAGGGATGGATCTGGTCAATAAAATATATCGCTCATCCTGGCTGCAT
TGTGGGCTGGTGGCAGGGCG

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTDNTRAIPRSILYTPALSLNRVVKALTYDADVHLIDLEDSVPGPSKAEGRIICRTALDNAPPSANIAVRINALGTVDAL
HDLVMLCGGGTAPGFIVMSMVRSPAEVVFLRETFASAGKHPEIYLTIETVEAITDLDDVAAVADGLIFGSADLAATLGVP
ITWSGMLAARQAMVLASARHGIGCIDTANYRLSEPEVLAHEIQNIRDLGFHGKATVHPSELEPINSALRPNEGALLAARR
ITEAVKAAQGGIALLDGHMVGPPFARMAHTTLGLGQAWAARFNRSNS

Sequences:

>Translated_287_residues
MTDNTRAIPRSILYTPALSLNRVVKALTYDADVHLIDLEDSVPGPSKAEGRIICRTALDNAPPSANIAVRINALGTVDAL
HDLVMLCGGGTAPGFIVMSMVRSPAEVVFLRETFASAGKHPEIYLTIETVEAITDLDDVAAVADGLIFGSADLAATLGVP
ITWSGMLAARQAMVLASARHGIGCIDTANYRLSEPEVLAHEIQNIRDLGFHGKATVHPSELEPINSALRPNEGALLAARR
ITEAVKAAQGGIALLDGHMVGPPFARMAHTTLGLGQAWAARFNRSNS
>Mature_286_residues
TDNTRAIPRSILYTPALSLNRVVKALTYDADVHLIDLEDSVPGPSKAEGRIICRTALDNAPPSANIAVRINALGTVDALH
DLVMLCGGGTAPGFIVMSMVRSPAEVVFLRETFASAGKHPEIYLTIETVEAITDLDDVAAVADGLIFGSADLAATLGVPI
TWSGMLAARQAMVLASARHGIGCIDTANYRLSEPEVLAHEIQNIRDLGFHGKATVHPSELEPINSALRPNEGALLAARRI
TEAVKAAQGGIALLDGHMVGPPFARMAHTTLGLGQAWAARFNRSNS

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=293, Percent_Identity=24.5733788395904, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30312; Mature: 30181

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNTRAIPRSILYTPALSLNRVVKALTYDADVHLIDLEDSVPGPSKAEGRIICRTALDN
CCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCC
APPSANIAVRINALGTVDALHDLVMLCGGGTAPGFIVMSMVRSPAEVVFLRETFASAGKH
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHEEEEHHHHHHCCCC
PEIYLTIETVEAITDLDDVAAVADGLIFGSADLAATLGVPITWSGMLAARQAMVLASARH
CEEEEEEEHHHHHHCHHHHHHHHHHHEECCCHHHHHCCCCEEEHHHHHHHHHHHHHHHCC
GIGCIDTANYRLSEPEVLAHEIQNIRDLGFHGKATVHPSELEPINSALRPNEGALLAARR
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHCHHHHHCCCCCCCCHHHHH
ITEAVKAAQGGIALLDGHMVGPPFARMAHTTLGLGQAWAARFNRSNS
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCCC
>Mature Secondary Structure 
TDNTRAIPRSILYTPALSLNRVVKALTYDADVHLIDLEDSVPGPSKAEGRIICRTALDN
CCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEEEECCC
APPSANIAVRINALGTVDALHDLVMLCGGGTAPGFIVMSMVRSPAEVVFLRETFASAGKH
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHEEEEHHHHHHCCCC
PEIYLTIETVEAITDLDDVAAVADGLIFGSADLAATLGVPITWSGMLAARQAMVLASARH
CEEEEEEEHHHHHHCHHHHHHHHHHHEECCCHHHHHCCCCEEEHHHHHHHHHHHHHHHCC
GIGCIDTANYRLSEPEVLAHEIQNIRDLGFHGKATVHPSELEPINSALRPNEGALLAARR
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHCHHHHHCCCCCCCCHHHHH
ITEAVKAAQGGIALLDGHMVGPPFARMAHTTLGLGQAWAARFNRSNS
HHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA