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The map label for this gene is nhaB

Identifier: 157370989

GI number: 157370989

Start: 3029847

End: 3031427

Strand: Direct

Name: nhaB

Synonym: Spro_2749

Alternate gene names: 157370989

Gene position: 3029847-3031427 (Clockwise)

Preceding gene: 157370987

Following gene: 157370990

Centisome position: 55.61

GC content: 54.4

Gene sequence:

>1581_bases
ATGGAAACGACCTTTCGCAGTGCCCTGGTGAAAAACTTCCTCGGGCAATCACCAGACTGGTACAAACTGGCCATTCTGGT
TTTCCTGGTGGTCAACCCACTGGTTTTCTTCCTGGTCGATCCGTTCATTGCGGGTTGGCTGTTAGTAGTCGAATTTATCT
TTACCCTGGCAATGGCGCTGAAGTGCTACCCACTGCTGCCGGGTGGTCTGCTGGCCATTGAGTCAGTGCTGATCGGCATG
ACCAGCCCCGATCGGGTGGGTGAGGAAATTGCCCATAATCTCGAAGTGCTGCTGCTGCTGATCTTTATGGTAGCCGGCAT
CTACTTTATGAAGCAGTTGCTGCTGTTCGTTTTCACCAAGCTACTGCTCAACATCCGCTCAAAAATGCTGCTTTCTTTGG
CATTCTGTTTTGCGGCGGCGCTGCTGTCAGCCTTCCTCGATGCATTGACGGTCGTTGCGGTGGTTATCAGCGTCGCTACC
GGATTTTATTCGATCTACCACAACGTGGCTTCAAACCGTTCTGACGGTAATGACGGTAATATTGATGTCGGTGACGATAG
CACCCTGGTCAGTGACGATCACAAACAGACGCTCGAGCAGTTCCGCGCCTTCTTGCGCAGCCTGCTGATGCACGCAGGCG
TCGGTACTGCGCTGGGCGGCGTGATGACCATGGTGGGTGAACCGCAGAACCTGATTATTGCCAAAAGCGCCGGCTGGGGA
TTTGTTGACTTCTTCCTGCGCATGGCGCCGGTTACCCTGCCGGTGTTTGCGTGCGGCCTGCTGGTTTGCTTACTGCTCGA
ACGCTTCGGCGTCTTCGGCTACGGAGCAAAACTGCCGGAACGCGTACGTGAGGTACTGACCGAGTTTGACCGCCAGGCTA
CCGCCGGACGCAGTAAACAAGAACAGGTCAGATTGGTCGTTCAGGCGCTAATCGGCATTTGGTTGGTGGTGGCGCTGGCC
TTTCATCTGGCGGAAGTAGGCCTGATTGGTCTTTCGGTCATTATTCTCGCCACTTCACTGTGCGGCGTCACCGATGAACA
CGCCATCGGTAAGGCCTTCCAGGAGGCGCTGCCTTTTACCGCGCTGCTGACGGTATTTTTCACCGTGGTGGCAGTAATTA
TTGAGCAACACCTGTTTACACCGATTATTCATTTTGTTCTGCAGGCGCAACCCTCTTCACAACTGGCGCTGTTTTATCTG
TTTAATGGCCTGCTGTCTTCGGTATCTGACAACGTGTTCGTCGGTACGGTATACATCAATGAAGCCCGTGCTGCATTTGA
AAACGGTGTGATTTCCCTGAAGCAGTTCGAAATGCTGGCGGTCGCCATTAATACCGGCACCAATCTGCCATCGGTCGCCA
CGCCGAATGGTCAGGCGGCTTTCCTGTTCCTGCTGACCTCTGCGCTGGCACCGCTGGTGCGTTTGTCTTACGGGCGGATG
GTATGGATGGCTTTACCCTATACTGTGGTATTAACCCTGGTGGGTCTGCTGTGCGTCCAGTTTACACTGGCACCTGCGAC
TGAGCTGCTGACCCAGTGGCATTGGCTGACGCTGCCATCGATTGAAGCCGCTACACACTGA

Upstream 100 bases:

>100_bases
AGAGAAATTTCTCATCGTAAAGCAAGCCCTTATTGCATCTGCCCCGGCGGCATTGTTAGGCTAGTATGAGACAACTTATT
TTCAGGACGTGTAACGCATA

Downstream 100 bases:

>100_bases
CACTGTTAACCCACTCCGCTGTCAGCGACTATTTTTGTGCTGAAACAGGCTCAGGTAGCTGGCAGCAGAGTTGAATTGGT
TTACACTGCCAGTTCAATTG

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaB

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
METTFRSALVKNFLGQSPDWYKLAILVFLVVNPLVFFLVDPFIAGWLLVVEFIFTLAMALKCYPLLPGGLLAIESVLIGM
TSPDRVGEEIAHNLEVLLLLIFMVAGIYFMKQLLLFVFTKLLLNIRSKMLLSLAFCFAAALLSAFLDALTVVAVVISVAT
GFYSIYHNVASNRSDGNDGNIDVGDDSTLVSDDHKQTLEQFRAFLRSLLMHAGVGTALGGVMTMVGEPQNLIIAKSAGWG
FVDFFLRMAPVTLPVFACGLLVCLLLERFGVFGYGAKLPERVREVLTEFDRQATAGRSKQEQVRLVVQALIGIWLVVALA
FHLAEVGLIGLSVIILATSLCGVTDEHAIGKAFQEALPFTALLTVFFTVVAVIIEQHLFTPIIHFVLQAQPSSQLALFYL
FNGLLSSVSDNVFVGTVYINEARAAFENGVISLKQFEMLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLVRLSYGRM
VWMALPYTVVLTLVGLLCVQFTLAPATELLTQWHWLTLPSIEAATH

Sequences:

>Translated_526_residues
METTFRSALVKNFLGQSPDWYKLAILVFLVVNPLVFFLVDPFIAGWLLVVEFIFTLAMALKCYPLLPGGLLAIESVLIGM
TSPDRVGEEIAHNLEVLLLLIFMVAGIYFMKQLLLFVFTKLLLNIRSKMLLSLAFCFAAALLSAFLDALTVVAVVISVAT
GFYSIYHNVASNRSDGNDGNIDVGDDSTLVSDDHKQTLEQFRAFLRSLLMHAGVGTALGGVMTMVGEPQNLIIAKSAGWG
FVDFFLRMAPVTLPVFACGLLVCLLLERFGVFGYGAKLPERVREVLTEFDRQATAGRSKQEQVRLVVQALIGIWLVVALA
FHLAEVGLIGLSVIILATSLCGVTDEHAIGKAFQEALPFTALLTVFFTVVAVIIEQHLFTPIIHFVLQAQPSSQLALFYL
FNGLLSSVSDNVFVGTVYINEARAAFENGVISLKQFEMLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLVRLSYGRM
VWMALPYTVVLTLVGLLCVQFTLAPATELLTQWHWLTLPSIEAATH
>Mature_526_residues
METTFRSALVKNFLGQSPDWYKLAILVFLVVNPLVFFLVDPFIAGWLLVVEFIFTLAMALKCYPLLPGGLLAIESVLIGM
TSPDRVGEEIAHNLEVLLLLIFMVAGIYFMKQLLLFVFTKLLLNIRSKMLLSLAFCFAAALLSAFLDALTVVAVVISVAT
GFYSIYHNVASNRSDGNDGNIDVGDDSTLVSDDHKQTLEQFRAFLRSLLMHAGVGTALGGVMTMVGEPQNLIIAKSAGWG
FVDFFLRMAPVTLPVFACGLLVCLLLERFGVFGYGAKLPERVREVLTEFDRQATAGRSKQEQVRLVVQALIGIWLVVALA
FHLAEVGLIGLSVIILATSLCGVTDEHAIGKAFQEALPFTALLTVFFTVVAVIIEQHLFTPIIHFVLQAQPSSQLALFYL
FNGLLSSVSDNVFVGTVYINEARAAFENGVISLKQFEMLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLVRLSYGRM
VWMALPYTVVLTLVGLLCVQFTLAPATELLTQWHWLTLPSIEAATH

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3067

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaB Na(+)/H(+) (TC 2.A.34) antiporter family

Homologues:

Organism=Escherichia coli, GI1787435, Length=516, Percent_Identity=76.1627906976744, Blast_Score=759, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAB_SERP5 (A8GFG0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478978.1
- STRING:   A8GFG0
- GeneID:   5607058
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2749
- eggNOG:   COG3067
- HOGENOM:   HBG296776
- OMA:   CFLVEKF
- ProtClustDB:   PRK09547
- BioCyc:   SPRO399741:SPRO_2749-MONOMER
- HAMAP:   MF_01599
- InterPro:   IPR004671
- InterPro:   IPR001991
- PRINTS:   PR00173
- TIGRFAMs:   TIGR00774

Pfam domain/function: PF06450 NhaB

EC number: NA

Molecular weight: Translated: 57404; Mature: 57404

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2643a1e4)-; HASH(0x263ac304)-; HASH(0x25ed6990)-; HASH(0x2639fb84)-; HASH(0x23808acc)-; HASH(0x2205f23c)-; HASH(0x25c3e064)-; HASH(0x261f4f84)-; HASH(0x25f2bf7c)-; HASH(0x261c1e08)-; HASH(0x263b2a08)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3 Proton [Periplasm]; Na (I) [Cytoplasm] [C]

Specific reaction: 3 Proton [Periplasm] + 2 Na (I) [Cytoplasm] = 2 Na (I) [Periplasm] + 3 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA