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The map label for this gene is pspF [H]

Identifier: 157370865

GI number: 157370865

Start: 2888558

End: 2889565

Strand: Direct

Name: pspF [H]

Synonym: Spro_2625

Alternate gene names: 157370865

Gene position: 2888558-2889565 (Clockwise)

Preceding gene: 157370860

Following gene: 157370869

Centisome position: 53.01

GC content: 58.43

Gene sequence:

>1008_bases
ATGAGCGAACAGTTAGAAAACCTGTTGGGCGAAGCGAATGCCTTTGTCGAAGTATTGGAGCAAGTCTCGCAACTGGCAAA
GTTGAACAAACCGGTGTTGGTGATCGGTGAGCGCGGCACCGGTAAAGAACTGATTGCCCACCGGTTGCATTACCTGTCCA
ACCGTTGGCAGGGGCCGTTTATCTCGCTTAACTGCGCGGCCCTGAATGAAAACCTGCTGGACTCCGAGCTGTTCGGTCAC
GAGGCCGGGGCCTTTACCGGCGCGCAAAAGCGCCATCTGGGCCGCTTTGAACGTGCCGACGGCGGCACGCTGTTTCTCGA
CGAACTGGCCACCGCCCCGATGCTGGTGCAGGAAAAATTGCTGCGGGTGATTGAATACGGCCATCTGGAGCGCGTCGGCG
GCAGCCAGCCGTTGCAGGTCGATGTGCGGTTGGTGTGTGCCACCAACGACGATCTTCCGGCACTGGCGGCGGCGGGCAAA
TTCCGCGCCGACCTGCTGGATCGGTTGGCATTCGACGTAGTGCAGTTACCGCCGCTGCGCCAGCGGCAGCAAGACATCAT
GTTGCTGGCCGAGCACTTTGCGATTCAGATGTGCCGTGAGCTGTCGCTGACACTGTTCCCCGGCTTTACCGAACGTGCCA
GAGACACCCTGCTCGGCTATCACTGGCCGGGCAACGTGCGTGAGCTTAAAAACGTGGTGGAACGTTCGATGTATCGGCAC
GGTACCAGCGACAGCGCGTTGGATCAGATAGTCATCAACCCCTTCGCCCAACGGGAAACTATGCCGCTGCCCATGGCGCG
CGAAACGCCAACTGAGCGGCCAGTCCTGCCGCTGGATTTGAAAGCCTGGCAGTTGGCGCAGGAAAAATCGCTAATCGAAG
CCGCACTTCAACAGGGAAGATTTAATCAGCGTAAGGCGGCGGAGCTGCTGGGGCTGACCTATCACCAATTACGTGGCATG
TTGAAAAAGCACGCGCTGTTGCAAAATGGCGAGGCAGAAGAGGAATAA

Upstream 100 bases:

>100_bases
CCAACTTTTATTTCATTGAATAATAACGATTATATATTTTTGACTCTCTGTGAGCATACTGTAGAGTGATTATTTTCACC
AAACATTGGCGAATTTCATC

Downstream 100 bases:

>100_bases
CAGGCAAAAAAAAAGCCAGCACCCGAGCTGGCTTAAAAAACACTGGAAGCAATGTGAGCAATGTCGTGCCTTTCACAGCA
GAGCCACAAACCGTGGCCTT

Product: phage shock protein operon transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSEQLENLLGEANAFVEVLEQVSQLAKLNKPVLVIGERGTGKELIAHRLHYLSNRWQGPFISLNCAALNENLLDSELFGH
EAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGHLERVGGSQPLQVDVRLVCATNDDLPALAAAGK
FRADLLDRLAFDVVQLPPLRQRQQDIMLLAEHFAIQMCRELSLTLFPGFTERARDTLLGYHWPGNVRELKNVVERSMYRH
GTSDSALDQIVINPFAQRETMPLPMARETPTERPVLPLDLKAWQLAQEKSLIEAALQQGRFNQRKAAELLGLTYHQLRGM
LKKHALLQNGEAEEE

Sequences:

>Translated_335_residues
MSEQLENLLGEANAFVEVLEQVSQLAKLNKPVLVIGERGTGKELIAHRLHYLSNRWQGPFISLNCAALNENLLDSELFGH
EAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGHLERVGGSQPLQVDVRLVCATNDDLPALAAAGK
FRADLLDRLAFDVVQLPPLRQRQQDIMLLAEHFAIQMCRELSLTLFPGFTERARDTLLGYHWPGNVRELKNVVERSMYRH
GTSDSALDQIVINPFAQRETMPLPMARETPTERPVLPLDLKAWQLAQEKSLIEAALQQGRFNQRKAAELLGLTYHQLRGM
LKKHALLQNGEAEEE
>Mature_334_residues
SEQLENLLGEANAFVEVLEQVSQLAKLNKPVLVIGERGTGKELIAHRLHYLSNRWQGPFISLNCAALNENLLDSELFGHE
AGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKLLRVIEYGHLERVGGSQPLQVDVRLVCATNDDLPALAAAGKF
RADLLDRLAFDVVQLPPLRQRQQDIMLLAEHFAIQMCRELSLTLFPGFTERARDTLLGYHWPGNVRELKNVVERSMYRHG
TSDSALDQIVINPFAQRETMPLPMARETPTERPVLPLDLKAWQLAQEKSLIEAALQQGRFNQRKAAELLGLTYHQLRGML
KKHALLQNGEAEEE

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=77.3006134969325, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1790437, Length=320, Percent_Identity=42.5, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1788550, Length=331, Percent_Identity=39.8791540785499, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI87082117, Length=243, Percent_Identity=48.559670781893, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789087, Length=246, Percent_Identity=46.3414634146341, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1790299, Length=228, Percent_Identity=50, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1789233, Length=330, Percent_Identity=38.4848484848485, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI87082152, Length=322, Percent_Identity=38.8198757763975, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1786524, Length=243, Percent_Identity=44.0329218106996, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788905, Length=324, Percent_Identity=34.8765432098765, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1787583, Length=344, Percent_Identity=33.7209302325581, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=36.6379310344828, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=28.6184210526316, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 37680; Mature: 37549

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLENLLGEANAFVEVLEQVSQLAKLNKPVLVIGERGTGKELIAHRLHYLSNRWQGPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCE
ISLNCAALNENLLDSELFGHEAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKL
EEEEHHHHCCHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCEEEHHHHCCCHHHHHHHH
LRVIEYGHLERVGGSQPLQVDVRLVCATNDDLPALAAAGKFRADLLDRLAFDVVQLPPLR
HHHHHHCCHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QRQQDIMLLAEHFAIQMCRELSLTLFPGFTERARDTLLGYHWPGNVRELKNVVERSMYRH
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHC
GTSDSALDQIVINPFAQRETMPLPMARETPTERPVLPLDLKAWQLAQEKSLIEAALQQGR
CCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
FNQRKAAELLGLTYHQLRGMLKKHALLQNGEAEEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEQLENLLGEANAFVEVLEQVSQLAKLNKPVLVIGERGTGKELIAHRLHYLSNRWQGPF
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCE
ISLNCAALNENLLDSELFGHEAGAFTGAQKRHLGRFERADGGTLFLDELATAPMLVQEKL
EEEEHHHHCCHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCEEEHHHHCCCHHHHHHHH
LRVIEYGHLERVGGSQPLQVDVRLVCATNDDLPALAAAGKFRADLLDRLAFDVVQLPPLR
HHHHHHCCHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QRQQDIMLLAEHFAIQMCRELSLTLFPGFTERARDTLLGYHWPGNVRELKNVVERSMYRH
HHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHC
GTSDSALDQIVINPFAQRETMPLPMARETPTERPVLPLDLKAWQLAQEKSLIEAALQQGR
CCCHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
FNQRKAAELLGLTYHQLRGMLKKHALLQNGEAEEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]