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The map label for this gene is fhlA [H]

Identifier: 157370680

GI number: 157370680

Start: 2672407

End: 2674473

Strand: Direct

Name: fhlA [H]

Synonym: Spro_2440

Alternate gene names: 157370680

Gene position: 2672407-2674473 (Clockwise)

Preceding gene: 157370679

Following gene: 157370683

Centisome position: 49.05

GC content: 56.12

Gene sequence:

>2067_bases
ATGTCGAATGCTCAATTAGAAAAACAAGAATTACTTGAAACGACCCGCACGCTGCTCAGTCAGCACAGCTTCGCCGATTT
GTTACGGCAGCTGCGCCAGGTGCTGCAACGTCAGCAACTGGCAGACCATGTGACGCTGGTGCTGTTTGATTTGCACAGTG
AGCGGGTCAGCTTTCATGGGCTGGATGCGCAACGGCAGCCGGTCATCTATCAGGATGAGACTTTGCTGGCCAATGGGCCG
GTGAGCCGGCTGCGACAAAGCCCGTTGCCGCAGCGCTGGCAGAGTGAGGATCTGCATGCACGTTACCCGCAAATCAACGC
CCTGGCGCTGTATCTGCCGTTTAACCAATATTGCCTGATGCCGCTGCACGCAGGCGGTAGTCTTATCGGCGGTTGTGAAC
TGTTACGCAGTAGCCCGCAACCCTTTAGTGATGCCAGTCTGGTGCAGTTGCAGACCCTGATGGAATTGGTTGCGCTGGCG
GTGGAACAGATCAGGTTGCGTGAGCAGGCGGAAAACCGCCAACGTCAGTTATGCCATGAGCGTGACGATTACCGCGTTTT
GGTCGACGTCACCAACGCAGTGTTATCGAAACTCGATCTGGATGATTTGATTGGCGAAATATCTACCGAGATCCACCGTT
TTTTCAAGATCGATGCCATCAGCATGGTGCTATGCGTCGATAAAACCGATCGGGTGACCATCTATTCCACCCACTATTTG
CAAGACGACGTGGTTGAACGTCAGCAGTACAGCGTGGCACTGGCCGGTACCTTATCCGAACAGGTGATGCAGAGCGGGGA
AATGCTGCTGCTCAATCTGAAGCATTCCGATCGTCTGGCGGCTTATGAACGCCAACTGTTCGATCTCTGGCAGGATCAAA
TCCAAACGCTGTGCCTCTTGCCGCTGGTGTTTGGCAACAAGACCCTGGGCGTATTGAAGCTGGCGCAATGCCAGCCGGAC
AACTTCAACGCCGCCAATCTGCGGGTGTTGCAGCAGATTGCCGAGCGTATCGCCATTGCGGTGGACAACGCGTTGGCCTA
CCAGGAAATCAGCCGCCTCAAAGAGAGCCTGGTGCATGAAAACCTCTATCTGACCGAGCAAATCAACGGCAACAACCCGG
ACTTTGGTGAGATTGTCGGGCGCAGCGTGGTGATGTCTGCGGTGCTTAAACAGGTTGAAATGGTGGCGAAAAGCGACTGT
TCGGTGCTGATCCTCGGCGAAACCGGCACCGGTAAAGAGTTGATTGCCCGCGCTATCCACAACCTGAGCGAGCGACGTGA
CCAGCGCATGGTGAAAATGAACTGTGCGGTGATGCCGACCGGGCTGTTGGAAAGCGATCTGTTTGGTCATGAAAAAGGCT
CATTCACCGGCGCAACCAACCAGCGGATGGGGCGCTTTGAGCTGGCGGATAAAGGAACGCTGTTTCTGGATGAAGTGGGT
GAAATCCCGGTGGAACTGCAGCCTAAACTGCTACGAGTGCTGCAGGAAAGGGAGTTTGAACGGGTAGGCGGCAACAAGGT
GATTTCGGTGGATGTGCGCCTGATTGCCGCCACCAACCGTGACCTGCAGCAAATGGTGGCCGACCGGGAGTTTCGCAGTG
ACCTTTTTTACCGGCTGAACGTGTTCCCGATCGTCATCCCGCCATTGCGTGAACGGCCGGAAGACATCCCGCAATTGGTG
AAGTTTCTCACCTACAAAATCGCGCGTCGGATGAAGCGCACCATCGACAGCATTCCGGCGGAGACGCTGCGCCTGCTGAG
TCAAATGCCCTGGCCGGGCAATGTGCGCGAGCTGGAGAATGTCATCGAACGGGCAGTATTGCTGACGCGTGGCACGGTAC
TTAACCTGGAGTTGCCGGAGCTGCGCTATCCGGTGCCGTCGATGGCCTTACCCTTGCCCTCGGCAGAGCCTGTCCAGGCG
GGTGAGGACGATCGCGAGCGGATTATCCGCGTATTGAAAGAAACCAACGGTGTGGTTGCCGGCCCACGTGGCGCCGCGCA
GCGCCTGGGTATCAAGCGCACTACACTGCTTTCACGCATGAAAAAATGGGGGATTGTGGTCAGGTAA

Upstream 100 bases:

>100_bases
CATTTGTTGATCCTGTCGGGGGAGAACCTTGTCGCCCAGCGTTACACTTTGTGACGTGACTCACTAAAATCATCATCGCG
ACTCAGGCAAGTGATAAACA

Downstream 100 bases:

>100_bases
TCGGCATCAGCGAATTAAAAGGCCCGAGTCCGGTGGCGTATCCGGCTCGGGCCTGGCGGGTCACTTAATTTGAATTAAGC
CGTCGCGTTATCGATCTCCA

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSNAQLEKQELLETTRTLLSQHSFADLLRQLRQVLQRQQLADHVTLVLFDLHSERVSFHGLDAQRQPVIYQDETLLANGP
VSRLRQSPLPQRWQSEDLHARYPQINALALYLPFNQYCLMPLHAGGSLIGGCELLRSSPQPFSDASLVQLQTLMELVALA
VEQIRLREQAENRQRQLCHERDDYRVLVDVTNAVLSKLDLDDLIGEISTEIHRFFKIDAISMVLCVDKTDRVTIYSTHYL
QDDVVERQQYSVALAGTLSEQVMQSGEMLLLNLKHSDRLAAYERQLFDLWQDQIQTLCLLPLVFGNKTLGVLKLAQCQPD
NFNAANLRVLQQIAERIAIAVDNALAYQEISRLKESLVHENLYLTEQINGNNPDFGEIVGRSVVMSAVLKQVEMVAKSDC
SVLILGETGTGKELIARAIHNLSERRDQRMVKMNCAVMPTGLLESDLFGHEKGSFTGATNQRMGRFELADKGTLFLDEVG
EIPVELQPKLLRVLQEREFERVGGNKVISVDVRLIAATNRDLQQMVADREFRSDLFYRLNVFPIVIPPLRERPEDIPQLV
KFLTYKIARRMKRTIDSIPAETLRLLSQMPWPGNVRELENVIERAVLLTRGTVLNLELPELRYPVPSMALPLPSAEPVQA
GEDDRERIIRVLKETNGVVAGPRGAAQRLGIKRTTLLSRMKKWGIVVR

Sequences:

>Translated_688_residues
MSNAQLEKQELLETTRTLLSQHSFADLLRQLRQVLQRQQLADHVTLVLFDLHSERVSFHGLDAQRQPVIYQDETLLANGP
VSRLRQSPLPQRWQSEDLHARYPQINALALYLPFNQYCLMPLHAGGSLIGGCELLRSSPQPFSDASLVQLQTLMELVALA
VEQIRLREQAENRQRQLCHERDDYRVLVDVTNAVLSKLDLDDLIGEISTEIHRFFKIDAISMVLCVDKTDRVTIYSTHYL
QDDVVERQQYSVALAGTLSEQVMQSGEMLLLNLKHSDRLAAYERQLFDLWQDQIQTLCLLPLVFGNKTLGVLKLAQCQPD
NFNAANLRVLQQIAERIAIAVDNALAYQEISRLKESLVHENLYLTEQINGNNPDFGEIVGRSVVMSAVLKQVEMVAKSDC
SVLILGETGTGKELIARAIHNLSERRDQRMVKMNCAVMPTGLLESDLFGHEKGSFTGATNQRMGRFELADKGTLFLDEVG
EIPVELQPKLLRVLQEREFERVGGNKVISVDVRLIAATNRDLQQMVADREFRSDLFYRLNVFPIVIPPLRERPEDIPQLV
KFLTYKIARRMKRTIDSIPAETLRLLSQMPWPGNVRELENVIERAVLLTRGTVLNLELPELRYPVPSMALPLPSAEPVQA
GEDDRERIIRVLKETNGVVAGPRGAAQRLGIKRTTLLSRMKKWGIVVR
>Mature_687_residues
SNAQLEKQELLETTRTLLSQHSFADLLRQLRQVLQRQQLADHVTLVLFDLHSERVSFHGLDAQRQPVIYQDETLLANGPV
SRLRQSPLPQRWQSEDLHARYPQINALALYLPFNQYCLMPLHAGGSLIGGCELLRSSPQPFSDASLVQLQTLMELVALAV
EQIRLREQAENRQRQLCHERDDYRVLVDVTNAVLSKLDLDDLIGEISTEIHRFFKIDAISMVLCVDKTDRVTIYSTHYLQ
DDVVERQQYSVALAGTLSEQVMQSGEMLLLNLKHSDRLAAYERQLFDLWQDQIQTLCLLPLVFGNKTLGVLKLAQCQPDN
FNAANLRVLQQIAERIAIAVDNALAYQEISRLKESLVHENLYLTEQINGNNPDFGEIVGRSVVMSAVLKQVEMVAKSDCS
VLILGETGTGKELIARAIHNLSERRDQRMVKMNCAVMPTGLLESDLFGHEKGSFTGATNQRMGRFELADKGTLFLDEVGE
IPVELQPKLLRVLQEREFERVGGNKVISVDVRLIAATNRDLQQMVADREFRSDLFYRLNVFPIVIPPLRERPEDIPQLVK
FLTYKIARRMKRTIDSIPAETLRLLSQMPWPGNVRELENVIERAVLLTRGTVLNLELPELRYPVPSMALPLPSAEPVQAG
EDDRERIIRVLKETNGVVAGPRGAAQRLGIKRTTLLSRMKKWGIVVR

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=684, Percent_Identity=60.9649122807018, Blast_Score=828, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=578, Percent_Identity=50.8650519031142, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI1788550, Length=319, Percent_Identity=48.58934169279, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790437, Length=333, Percent_Identity=45.3453453453453, Blast_Score=276, Evalue=4e-75,
Organism=Escherichia coli, GI87082152, Length=350, Percent_Identity=41.7142857142857, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788905, Length=254, Percent_Identity=49.2125984251969, Blast_Score=241, Evalue=9e-65,
Organism=Escherichia coli, GI1789233, Length=331, Percent_Identity=39.8791540785499, Blast_Score=235, Evalue=7e-63,
Organism=Escherichia coli, GI1790299, Length=282, Percent_Identity=45.3900709219858, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=46.9827586206897, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786524, Length=327, Percent_Identity=39.7553516819572, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787583, Length=320, Percent_Identity=35.625, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1789828, Length=346, Percent_Identity=34.971098265896, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=35.1351351351351, Blast_Score=149, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78116; Mature: 77984

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAQLEKQELLETTRTLLSQHSFADLLRQLRQVLQRQQLADHVTLVLFDLHSERVSFHG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHEECC
LDAQRQPVIYQDETLLANGPVSRLRQSPLPQRWQSEDLHARYPQINALALYLPFNQYCLM
CCCCCCCEEECCCEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEE
PLHAGGSLIGGCELLRSSPQPFSDASLVQLQTLMELVALAVEQIRLREQAENRQRQLCHE
EECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDDYRVLVDVTNAVLSKLDLDDLIGEISTEIHRFFKIDAISMVLCVDKTDRVTIYSTHYL
CCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEHHH
QDDVVERQQYSVALAGTLSEQVMQSGEMLLLNLKHSDRLAAYERQLFDLWQDQIQTLCLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PLVFGNKTLGVLKLAQCQPDNFNAANLRVLQQIAERIAIAVDNALAYQEISRLKESLVHE
HHHHCCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLYLTEQINGNNPDFGEIVGRSVVMSAVLKQVEMVAKSDCSVLILGETGTGKELIARAIH
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
NLSERRDQRMVKMNCAVMPTGLLESDLFGHEKGSFTGATNQRMGRFELADKGTLFLDEVG
HHHHHHHHHHHHHHCEECCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEHHHC
EIPVELQPKLLRVLQEREFERVGGNKVISVDVRLIAATNRDLQQMVADREFRSDLFYRLN
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHEEE
VFPIVIPPLRERPEDIPQLVKFLTYKIARRMKRTIDSIPAETLRLLSQMPWPGNVRELEN
EEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
VIERAVLLTRGTVLNLELPELRYPVPSMALPLPSAEPVQAGEDDRERIIRVLKETNGVVA
HHHHHHHHHCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
GPRGAAQRLGIKRTTLLSRMKKWGIVVR
CCCCHHHHCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNAQLEKQELLETTRTLLSQHSFADLLRQLRQVLQRQQLADHVTLVLFDLHSERVSFHG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHEECC
LDAQRQPVIYQDETLLANGPVSRLRQSPLPQRWQSEDLHARYPQINALALYLPFNQYCLM
CCCCCCCEEECCCEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEE
PLHAGGSLIGGCELLRSSPQPFSDASLVQLQTLMELVALAVEQIRLREQAENRQRQLCHE
EECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDDYRVLVDVTNAVLSKLDLDDLIGEISTEIHRFFKIDAISMVLCVDKTDRVTIYSTHYL
CCCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEHHH
QDDVVERQQYSVALAGTLSEQVMQSGEMLLLNLKHSDRLAAYERQLFDLWQDQIQTLCLL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
PLVFGNKTLGVLKLAQCQPDNFNAANLRVLQQIAERIAIAVDNALAYQEISRLKESLVHE
HHHHCCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLYLTEQINGNNPDFGEIVGRSVVMSAVLKQVEMVAKSDCSVLILGETGTGKELIARAIH
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHH
NLSERRDQRMVKMNCAVMPTGLLESDLFGHEKGSFTGATNQRMGRFELADKGTLFLDEVG
HHHHHHHHHHHHHHCEECCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEHHHC
EIPVELQPKLLRVLQEREFERVGGNKVISVDVRLIAATNRDLQQMVADREFRSDLFYRLN
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHEEE
VFPIVIPPLRERPEDIPQLVKFLTYKIARRMKRTIDSIPAETLRLLSQMPWPGNVRELEN
EEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
VIERAVLLTRGTVLNLELPELRYPVPSMALPLPSAEPVQAGEDDRERIIRVLKETNGVVA
HHHHHHHHHCCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEE
GPRGAAQRLGIKRTTLLSRMKKWGIVVR
CCCCHHHHCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]