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The map label for this gene is sseA [H]

Identifier: 157369675

GI number: 157369675

Start: 1562528

End: 1563373

Strand: Reverse

Name: sseA [H]

Synonym: Spro_1432

Alternate gene names: 157369675

Gene position: 1563373-1562528 (Counterclockwise)

Preceding gene: 157369676

Following gene: 157369673

Centisome position: 28.69

GC content: 58.39

Gene sequence:

>846_bases
ATGAACTCTCCGTTTCTGGTTACTCCGCAATGGCTTGCGCAGCATATCAACGATGAAAACCTGGTAGTGGTTGATGTCAG
GATGTCCCCGGTCGGCCTGGTGCCGAAAAAAGACATGCTCGCCGAATTTGAACGCGGCCATATCCCCGGCGCGGTGTATT
TTGACATCGATGACGTCGCGGATAAAAACACCGCATTGCCGCATATGCTGCCGACGGCAGAGGAATTCAGTGCGGCGGTG
GGTAAATTGGGCATCAGCGAACAACAGACCATCGTGTTTTATGACGAAGGCAACCAGTTCTCGGCCCCGCGCGGTTGGTG
GACCTTCCGCAACTTTGGCGCGCAGCAGGTTTATGTGTTGGACGAAGGCCTGAACGGCTGGACAGCTCTGGGCCAACCGC
TGGCTACCGGCGCGGCTCAGCGCACGCCGCAAACCTTCAACGCCCGCTTTAATGCGGACGCGGTAGTCAATATGCAGCAG
GTGGAACAGGCGCTGAACAGCGAAGTGCAAATCCTTGATGCGCGCGCCGCACCGCGTTTTTACGCCGAAGCACCAGAACC
GCGCCCCGGTCTGCACCGCGGCCATATTCCCGGCAGTATCAATATTCCTTACGGCGAACTGCTGGAAAACGGCCGTTTCA
AATCGCTGGAAGCACTGAAGCAAACCTTCAGCGATAAGGGCGTGAGTCTCGACGGACCCATCATCACCAGCTGCGGCTCC
GGCGTCACGGCAGCGGTGCTGGCTTTCGGCCTGCTGTCACTCGGTGCACCGCAGGTGAAACTGTATGACGGTGCCTGGAC
CGAATGGGGTGCGTTAAGCGGTAAGCAGCCGATCGAGCAGGACTAG

Upstream 100 bases:

>100_bases
TCGGGCTTTGGCCGCCATTGTCGCCCCCCTGAACGCCGAGGCAACGCCAATTATTTAAGGTATAGTGAAAAACCGTTTTC
ACCACAGGCGAATAATAACG

Downstream 100 bases:

>100_bases
CTGTTGATCCGATCGGCGACGATCTGCGCCAGACGCTGGATCGCCGCCGAGGGGTTTTCTTCGGCACGGTGCAGCACCAC
AGCTAACGGGTTTAGCGCCG

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNSPFLVTPQWLAQHINDENLVVVDVRMSPVGLVPKKDMLAEFERGHIPGAVYFDIDDVADKNTALPHMLPTAEEFSAAV
GKLGISEQQTIVFYDEGNQFSAPRGWWTFRNFGAQQVYVLDEGLNGWTALGQPLATGAAQRTPQTFNARFNADAVVNMQQ
VEQALNSEVQILDARAAPRFYAEAPEPRPGLHRGHIPGSINIPYGELLENGRFKSLEALKQTFSDKGVSLDGPIITSCGS
GVTAAVLAFGLLSLGAPQVKLYDGAWTEWGALSGKQPIEQD

Sequences:

>Translated_281_residues
MNSPFLVTPQWLAQHINDENLVVVDVRMSPVGLVPKKDMLAEFERGHIPGAVYFDIDDVADKNTALPHMLPTAEEFSAAV
GKLGISEQQTIVFYDEGNQFSAPRGWWTFRNFGAQQVYVLDEGLNGWTALGQPLATGAAQRTPQTFNARFNADAVVNMQQ
VEQALNSEVQILDARAAPRFYAEAPEPRPGLHRGHIPGSINIPYGELLENGRFKSLEALKQTFSDKGVSLDGPIITSCGS
GVTAAVLAFGLLSLGAPQVKLYDGAWTEWGALSGKQPIEQD
>Mature_281_residues
MNSPFLVTPQWLAQHINDENLVVVDVRMSPVGLVPKKDMLAEFERGHIPGAVYFDIDDVADKNTALPHMLPTAEEFSAAV
GKLGISEQQTIVFYDEGNQFSAPRGWWTFRNFGAQQVYVLDEGLNGWTALGQPLATGAAQRTPQTFNARFNADAVVNMQQ
VEQALNSEVQILDARAAPRFYAEAPEPRPGLHRGHIPGSINIPYGELLENGRFKSLEALKQTFSDKGVSLDGPIITSCGS
GVTAAVLAFGLLSLGAPQVKLYDGAWTEWGALSGKQPIEQD

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=274, Percent_Identity=39.7810218978102, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI61835204, Length=274, Percent_Identity=39.7810218978102, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI194473681, Length=274, Percent_Identity=39.7810218978102, Blast_Score=209, Evalue=2e-54,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=36.2637362637363, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=47.5, Blast_Score=266, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI71997283, Length=299, Percent_Identity=26.4214046822742, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17561888, Length=298, Percent_Identity=26.8456375838926, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17543836, Length=293, Percent_Identity=24.2320819112628, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17543838, Length=293, Percent_Identity=24.2320819112628, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=242, Percent_Identity=26.8595041322314, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI115534702, Length=198, Percent_Identity=30.3030303030303, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324825, Length=257, Percent_Identity=33.4630350194552, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30579; Mature: 30579

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPFLVTPQWLAQHINDENLVVVDVRMSPVGLVPKKDMLAEFERGHIPGAVYFDIDDVA
CCCCEEECHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECHHHC
DKNTALPHMLPTAEEFSAAVGKLGISEQQTIVFYDEGNQFSAPRGWWTFRNFGAQQVYVL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCHHHCCCCCEEEEE
DEGLNGWTALGQPLATGAAQRTPQTFNARFNADAVVNMQQVEQALNSEVQILDARAAPRF
ECCCCCHHHHCCHHHCCCHHCCCHHHCCEECCHHEECHHHHHHHHCCCEEEEECCCCCCH
YAEAPEPRPGLHRGHIPGSINIPYGELLENGRFKSLEALKQTFSDKGVSLDGPIITSCGS
HCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCC
GVTAAVLAFGLLSLGAPQVKLYDGAWTEWGALSGKQPIEQD
CHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSPFLVTPQWLAQHINDENLVVVDVRMSPVGLVPKKDMLAEFERGHIPGAVYFDIDDVA
CCCCEEECHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECHHHC
DKNTALPHMLPTAEEFSAAVGKLGISEQQTIVFYDEGNQFSAPRGWWTFRNFGAQQVYVL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCHHHCCCCCEEEEE
DEGLNGWTALGQPLATGAAQRTPQTFNARFNADAVVNMQQVEQALNSEVQILDARAAPRF
ECCCCCHHHHCCHHHCCCHHCCCHHHCCEECCHHEECHHHHHHHHCCCEEEEECCCCCCH
YAEAPEPRPGLHRGHIPGSINIPYGELLENGRFKSLEALKQTFSDKGVSLDGPIITSCGS
HCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCC
GVTAAVLAFGLLSLGAPQVKLYDGAWTEWGALSGKQPIEQD
CHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]