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The map label for this gene is yafC [C]

Identifier: 157369642

GI number: 157369642

Start: 1525181

End: 1526071

Strand: Reverse

Name: yafC [C]

Synonym: Spro_1399

Alternate gene names: 157369642

Gene position: 1526071-1525181 (Counterclockwise)

Preceding gene: 157369647

Following gene: 157369641

Centisome position: 28.01

GC content: 63.86

Gene sequence:

>891_bases
ATGGACCGATTAACCAGCATGGCGGTGTTTGTGCGGGTGGTGGAAAAAGGCAGCTTCGTTGCCGCCGCCGAGGAAATGGC
AATCTCCCCGACCATGGTCGGCAAACACATCCGCTACCTGGAGCAACGGCTGAATGCGCCGCTGCTGAACCGTACCACCC
GCAGACAGGGGCTGACCGAGACCGGCCGGGTATTCTACGAACGTTGCCGCCGCCTGCTGGCCGACGCCGATGCCGCCGAA
GCCAGTGCCCGGGCGCTATTGAGCTCCCCCAGCGGCCTGCTGCGCATCAGTGCCCCCGTCACTTTCGGCAACCGCGTACT
GACGCCGATCCTCACCGAGTTCCTGCAGCGGCACCCGGAGGTAGAAGCGGAAGTGGTGCTGTCGGACCGCAAGGTAGACT
TGATTGAAGAAGGCTACGAGGCGGCGTTTCGCATCGGGCCGGTGGCAGACGATGGCCTGGTGGCCCGCGCCCTGCCCGAT
TACCGCATGACGCTGGCCGCCTCACCGGACTATCTGGCCCAGCAGGGTGTGCCAACCCAGCCGGAGCAACTCAGCGCACA
CAGCTGCTTTGGTTTTAGTCAGTGGGACGGCAACCATTTCTGGCGCTTGCAGGGACCAACGGGGGAAATCAGCGTGCCGG
TCAGGCCGCGGCTGCGGATGGACTCTGGCGAAGGGGTACGTCGGGCGGCGCTGGCCGGTTTCGGTATCGCGCTGCATGCC
TGCATGCTGCTGGATGAAGATATTGCCAGCGGCCGATTGGTCCGGGTGCTGCCGGATTATTCACCGCTATCGCGGCCAAT
GCATCTGGTCTATCTGCCCGAACGACGGCAGTCACCCAAGCTGGAAGCTTTTATCGCCCTGACCGTCGCCAGACTCTGGG
GCCGCGTTTAA

Upstream 100 bases:

>100_bases
CCGCCAATAACACACTGACGGTCGATAGAATGATTATCGCAAAGCGCTATAATCAGGAGAAGTGGCCCACAGAGCATTCA
GAAAACACCAGGGGTTTATA

Downstream 100 bases:

>100_bases
AATAAGTAAATCAACACAGGAGTTTCCATGATCACTATCCGAGCACGCGCAGCACAAGACAATGCGCAACTGGCGGAGAT
TTGGCAGCGTTCGGTACGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDRLTSMAVFVRVVEKGSFVAAAEEMAISPTMVGKHIRYLEQRLNAPLLNRTTRRQGLTETGRVFYERCRRLLADADAAE
ASARALLSSPSGLLRISAPVTFGNRVLTPILTEFLQRHPEVEAEVVLSDRKVDLIEEGYEAAFRIGPVADDGLVARALPD
YRMTLAASPDYLAQQGVPTQPEQLSAHSCFGFSQWDGNHFWRLQGPTGEISVPVRPRLRMDSGEGVRRAALAGFGIALHA
CMLLDEDIASGRLVRVLPDYSPLSRPMHLVYLPERRQSPKLEAFIALTVARLWGRV

Sequences:

>Translated_296_residues
MDRLTSMAVFVRVVEKGSFVAAAEEMAISPTMVGKHIRYLEQRLNAPLLNRTTRRQGLTETGRVFYERCRRLLADADAAE
ASARALLSSPSGLLRISAPVTFGNRVLTPILTEFLQRHPEVEAEVVLSDRKVDLIEEGYEAAFRIGPVADDGLVARALPD
YRMTLAASPDYLAQQGVPTQPEQLSAHSCFGFSQWDGNHFWRLQGPTGEISVPVRPRLRMDSGEGVRRAALAGFGIALHA
CMLLDEDIASGRLVRVLPDYSPLSRPMHLVYLPERRQSPKLEAFIALTVARLWGRV
>Mature_296_residues
MDRLTSMAVFVRVVEKGSFVAAAEEMAISPTMVGKHIRYLEQRLNAPLLNRTTRRQGLTETGRVFYERCRRLLADADAAE
ASARALLSSPSGLLRISAPVTFGNRVLTPILTEFLQRHPEVEAEVVLSDRKVDLIEEGYEAAFRIGPVADDGLVARALPD
YRMTLAASPDYLAQQGVPTQPEQLSAHSCFGFSQWDGNHFWRLQGPTGEISVPVRPRLRMDSGEGVRRAALAGFGIALHA
CMLLDEDIASGRLVRVLPDYSPLSRPMHLVYLPERRQSPKLEAFIALTVARLWGRV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=280, Percent_Identity=35.3571428571429, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=34.1463414634146, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI87081978, Length=258, Percent_Identity=37.984496124031, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI145693193, Length=279, Percent_Identity=34.0501792114695, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1789440, Length=275, Percent_Identity=32.7272727272727, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1787128, Length=286, Percent_Identity=30.4195804195804, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=29.8932384341637, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788706, Length=287, Percent_Identity=24.390243902439, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786448, Length=239, Percent_Identity=25.9414225941423, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=25.6055363321799, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI157672245, Length=125, Percent_Identity=29.6, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=283, Percent_Identity=22.9681978798587, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32847; Mature: 32847

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLTSMAVFVRVVEKGSFVAAAEEMAISPTMVGKHIRYLEQRLNAPLLNRTTRRQGLTE
CCHHHHHHHHHHHHHCCCEEEHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
TGRVFYERCRRLLADADAAEASARALLSSPSGLLRISAPVTFGNRVLTPILTEFLQRHPE
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
VEAEVVLSDRKVDLIEEGYEAAFRIGPVADDGLVARALPDYRMTLAASPDYLAQQGVPTQ
CCEEEEECCCCHHHHHHCCHHHEEECCCCCCCCHHHCCCCCEEEEECCCCHHHHCCCCCC
PEQLSAHSCFGFSQWDGNHFWRLQGPTGEISVPVRPRLRMDSGEGVRRAALAGFGIALHA
HHHCCHHHCCCCCCCCCCEEEEEECCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHH
CMLLDEDIASGRLVRVLPDYSPLSRPMHLVYLPERRQSPKLEAFIALTVARLWGRV
HHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRLTSMAVFVRVVEKGSFVAAAEEMAISPTMVGKHIRYLEQRLNAPLLNRTTRRQGLTE
CCHHHHHHHHHHHHHCCCEEEHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
TGRVFYERCRRLLADADAAEASARALLSSPSGLLRISAPVTFGNRVLTPILTEFLQRHPE
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCC
VEAEVVLSDRKVDLIEEGYEAAFRIGPVADDGLVARALPDYRMTLAASPDYLAQQGVPTQ
CCEEEEECCCCHHHHHHCCHHHEEECCCCCCCCHHHCCCCCEEEEECCCCHHHHCCCCCC
PEQLSAHSCFGFSQWDGNHFWRLQGPTGEISVPVRPRLRMDSGEGVRRAALAGFGIALHA
HHHCCHHHCCCCCCCCCCEEEEEECCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHH
CMLLDEDIASGRLVRVLPDYSPLSRPMHLVYLPERRQSPKLEAFIALTVARLWGRV
HHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]