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The map label for this gene is nagB

Identifier: 157369470

GI number: 157369470

Start: 1346956

End: 1347756

Strand: Reverse

Name: nagB

Synonym: Spro_1227

Alternate gene names: 157369470

Gene position: 1347756-1346956 (Counterclockwise)

Preceding gene: 157369479

Following gene: 157369469

Centisome position: 24.73

GC content: 52.56

Gene sequence:

>801_bases
ATGAGACTTATCCCACTGAAAGATACTGCACAAGTCGGCAAATGGGCCGCCCGCCATATCGTCCAGCGCATCAACGCATT
CAAGCCTACTGCGGAGCGTCCGTTTGTGCTCGGCCTGCCAACCGGCGGTACCCCGCTGGAAGCCTACAAACATCTGATTG
CGATGCACAAAGCAGGTGAAGTAAGCTTTAAGCATGTGGTGACTTTCAACATGGACGAATACGTTGGCCTGCCGCAGGAA
CACCCGGAAAGCTATCATACCTTCATGTACCGTAATTTCTTTGATCACGTTGATATCCCAAGTGAAAATATCAACCTGCT
GAATGGTAATGCGCCGGACGTTGACGCCGAGTGCCGCCAGTACGAAGCGAAAATCAAATCCTACGGCAAGATCAACCTGT
TCATGGGTGGCGTTGGCATCGACGGTCATATCGCGTTTAACGAACCGGCTTCATCGCTGGCATCCCGTACTCGCATCAAA
ACCCTGACTGAAGATACCCGTATCGCCAACTCCCGCTTCTTTGGCGGTGATGTCAGCCTGGTGCCTAAATTTGCCCTGAC
CGTGGGCGTGGGCACGCTGCTTGATGCAGAAGAAGTGATGATTCTGGTGACCGGCCATGCCAAGGCACAGGCGCTGGAAG
CCGCAGTGGAAGGCAATATCAACCACATGTGGACCATCAGTTGCCTGCAGTTGCATGCCAAGGCCGTTGTGGTGTGCGAC
GAGCCATCCACCATGGAACTGAAAGTCAAAACCGTTAAATATTTCCGCGAGTTAGAAGCGGAAAGCGTTAAGAGTCTTTA
A

Upstream 100 bases:

>100_bases
TGAGACATCACGTGAGAATCATAAAGCTGGGTTAGGCTTAAGACTCTCGTTCAGGCTCGAACCAGTATAGCTTTAGTTTA
CTTACCCCAAGAGGTGTTAG

Downstream 100 bases:

>100_bases
TTCTTGCAGGGGGCTACGATGTTCGCTTTAACCCACGGCCGTATTTATACCGGCCACGACGTACTTGACGACCACGCAGT
TATTATCGCTGATGGGCTGA

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRLIPLKDTAQVGKWAARHIVQRINAFKPTAERPFVLGLPTGGTPLEAYKHLIAMHKAGEVSFKHVVTFNMDEYVGLPQE
HPESYHTFMYRNFFDHVDIPSENINLLNGNAPDVDAECRQYEAKIKSYGKINLFMGGVGIDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFGGDVSLVPKFALTVGVGTLLDAEEVMILVTGHAKAQALEAAVEGNINHMWTISCLQLHAKAVVVCD
EPSTMELKVKTVKYFRELEAESVKSL

Sequences:

>Translated_266_residues
MRLIPLKDTAQVGKWAARHIVQRINAFKPTAERPFVLGLPTGGTPLEAYKHLIAMHKAGEVSFKHVVTFNMDEYVGLPQE
HPESYHTFMYRNFFDHVDIPSENINLLNGNAPDVDAECRQYEAKIKSYGKINLFMGGVGIDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFGGDVSLVPKFALTVGVGTLLDAEEVMILVTGHAKAQALEAAVEGNINHMWTISCLQLHAKAVVVCD
EPSTMELKVKTVKYFRELEAESVKSL
>Mature_266_residues
MRLIPLKDTAQVGKWAARHIVQRINAFKPTAERPFVLGLPTGGTPLEAYKHLIAMHKAGEVSFKHVVTFNMDEYVGLPQE
HPESYHTFMYRNFFDHVDIPSENINLLNGNAPDVDAECRQYEAKIKSYGKINLFMGGVGIDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFGGDVSLVPKFALTVGVGTLLDAEEVMILVTGHAKAQALEAAVEGNINHMWTISCLQLHAKAVVVCD
EPSTMELKVKTVKYFRELEAESVKSL

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=258, Percent_Identity=60.8527131782946, Blast_Score=333, Evalue=7e-92,
Organism=Homo sapiens, GI19923881, Length=258, Percent_Identity=59.6899224806201, Blast_Score=330, Evalue=9e-91,
Organism=Escherichia coli, GI1786893, Length=266, Percent_Identity=82.7067669172932, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI48994958, Length=240, Percent_Identity=27.9166666666667, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789530, Length=212, Percent_Identity=26.8867924528302, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554876, Length=258, Percent_Identity=58.1395348837209, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24581960, Length=256, Percent_Identity=60.15625, Blast_Score=339, Evalue=9e-94,
Organism=Drosophila melanogaster, GI19920764, Length=256, Percent_Identity=60.15625, Blast_Score=339, Evalue=9e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_SERP5 (A8GB41)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477459.1
- ProteinModelPortal:   A8GB41
- SMR:   A8GB41
- STRING:   A8GB41
- GeneID:   5603588
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1227
- eggNOG:   COG0363
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- BioCyc:   SPRO399741:SPRO_1227-MONOMER
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 29496; Mature: 29496

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 141-141 ACT_SITE 143-143 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIPLKDTAQVGKWAARHIVQRINAFKPTAERPFVLGLPTGGTPLEAYKHLIAMHKAGE
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
VSFKHVVTFNMDEYVGLPQEHPESYHTFMYRNFFDHVDIPSENINLLNGNAPDVDAECRQ
CEEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHH
YEAKIKSYGKINLFMGGVGIDGHIAFNEPASSLASRTRIKTLTEDTRIANSRFFGGDVSL
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCCHHH
VPKFALTVGVGTLLDAEEVMILVTGHAKAQALEAAVEGNINHMWTISCLQLHAKAVVVCD
HHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEHHEEEEECEEEEEEC
EPSTMELKVKTVKYFRELEAESVKSL
CCCCEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLIPLKDTAQVGKWAARHIVQRINAFKPTAERPFVLGLPTGGTPLEAYKHLIAMHKAGE
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
VSFKHVVTFNMDEYVGLPQEHPESYHTFMYRNFFDHVDIPSENINLLNGNAPDVDAECRQ
CEEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHH
YEAKIKSYGKINLFMGGVGIDGHIAFNEPASSLASRTRIKTLTEDTRIANSRFFGGDVSL
HHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCCHHH
VPKFALTVGVGTLLDAEEVMILVTGHAKAQALEAAVEGNINHMWTISCLQLHAKAVVVCD
HHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEHHEEEEECEEEEEEC
EPSTMELKVKTVKYFRELEAESVKSL
CCCCEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA