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The map label for this gene is opuAB [H]

Identifier: 157369046

GI number: 157369046

Start: 894976

End: 895821

Strand: Reverse

Name: opuAB [H]

Synonym: Spro_0801

Alternate gene names: 157369046

Gene position: 895821-894976 (Counterclockwise)

Preceding gene: 157369047

Following gene: 157369045

Centisome position: 16.44

GC content: 60.28

Gene sequence:

>846_bases
ATGTTTCCAGATCGTTTTACTTTCTCCATCGCCGATTGGATCAATCGCTGGGTCGACGTGCTGGTCAACAACTATGGCGA
TATGTTCCGCAAGATCTCCGACACCTTGCTGTGGGCGGTGATCCACCTCGAAAGCCTGCTGCGCGCCACCCCATGGTGGG
TGATGCTGGCCGTGGTCGGGCTACTGGCCTGGCACGCCACCCGCCGTTGGCTGCCGACGGTGGTGATCGTCGGCCTGTTG
CTGCTGGTCGGTACCGCAGGGATGTGGGACAAATTGATGCAAACCCTGGCGCTGGTGCTGGTCGCCACGCTGCTGGCGGT
GATTATCGGCATTCCGCAAGGCATATTGGCGGCGCGTAGCGATCGCGTACGGGCCGTAATGATGCCGCTGATGGACGTGA
TGCAAACCATGCCGAGCTTCGTCTACCTGATCCCGGTGTTGATGCTGTTCGGGCTGGGCAAGGTGCCGGCAATTCTGGCG
ACGGTGATCTACGCCACCCCACCGCTGATCCGCCTGACCGATTTGGGTATCCGCCAGGTGGACAAAGAAGTGATGGAATC
CGTCACTGCCTTTGGCGCCAACCGCTGGCAGAAACTGTTCGGCGTGCAGTTGCCGCTGGCGCTGCCAAGCATCATGGCCG
GTATCAACCAGACCACCATGATGTCGCTGTCAATGGTGGTTGTGGCCTCGATGATCGGTGCACGCGGGCTGGGCGAAGAC
GTGCTGGTGGGCATTCAGACACTGAACGTCGGTCTGGGCCTGGAGGCAGGTCTGGCGATCGTTATTCTCGCCGTAGTCAT
TGACCGTATCACCCAGGCTTATGGCCGCACCGCCATTGCGAGGTAA

Upstream 100 bases:

>100_bases
ACGCAGCGAAAAAAATTCAGGCCTCCTTGTAATCGCTTTGGCATTCAGTAAACACCAACCCGGAGCCTGTGCGCTTCGGG
CCTGACAGGGGTAAATACTT

Downstream 100 bases:

>100_bases
CCCCGATGAACAAAATCGAAATCAAGAACGTCTACAAGATCTTCGGTCACAAAACGGCGGCGGCGCTGGCAATGAGCCAG
CAGGGTAAAACCAAGCAGGA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MFPDRFTFSIADWINRWVDVLVNNYGDMFRKISDTLLWAVIHLESLLRATPWWVMLAVVGLLAWHATRRWLPTVVIVGLL
LLVGTAGMWDKLMQTLALVLVATLLAVIIGIPQGILAARSDRVRAVMMPLMDVMQTMPSFVYLIPVLMLFGLGKVPAILA
TVIYATPPLIRLTDLGIRQVDKEVMESVTAFGANRWQKLFGVQLPLALPSIMAGINQTTMMSLSMVVVASMIGARGLGED
VLVGIQTLNVGLGLEAGLAIVILAVVIDRITQAYGRTAIAR

Sequences:

>Translated_281_residues
MFPDRFTFSIADWINRWVDVLVNNYGDMFRKISDTLLWAVIHLESLLRATPWWVMLAVVGLLAWHATRRWLPTVVIVGLL
LLVGTAGMWDKLMQTLALVLVATLLAVIIGIPQGILAARSDRVRAVMMPLMDVMQTMPSFVYLIPVLMLFGLGKVPAILA
TVIYATPPLIRLTDLGIRQVDKEVMESVTAFGANRWQKLFGVQLPLALPSIMAGINQTTMMSLSMVVVASMIGARGLGED
VLVGIQTLNVGLGLEAGLAIVILAVVIDRITQAYGRTAIAR
>Mature_281_residues
MFPDRFTFSIADWINRWVDVLVNNYGDMFRKISDTLLWAVIHLESLLRATPWWVMLAVVGLLAWHATRRWLPTVVIVGLL
LLVGTAGMWDKLMQTLALVLVATLLAVIIGIPQGILAARSDRVRAVMMPLMDVMQTMPSFVYLIPVLMLFGLGKVPAILA
TVIYATPPLIRLTDLGIRQVDKEVMESVTAFGANRWQKLFGVQLPLALPSIMAGINQTTMMSLSMVVVASMIGARGLGED
VLVGIQTLNVGLGLEAGLAIVILAVVIDRITQAYGRTAIAR

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=270, Percent_Identity=45.5555555555556, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1788451, Length=195, Percent_Identity=34.3589743589744, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788449, Length=177, Percent_Identity=30.5084745762712, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786564, Length=173, Percent_Identity=31.2138728323699, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30804; Mature: 30804

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]