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The map label for this gene is puuA [H]

Identifier: 157368829

GI number: 157368829

Start: 649019

End: 650416

Strand: Reverse

Name: puuA [H]

Synonym: Spro_0584

Alternate gene names: 157368829

Gene position: 650416-649019 (Counterclockwise)

Preceding gene: 157368831

Following gene: 157368828

Centisome position: 11.94

GC content: 60.66

Gene sequence:

>1398_bases
ATGGCCTCACGAGACGGATGTTTCATGTATTCTAATATTATTGGCTTCAACCCAATGCTGGCGGATATTACCGCCGCGCA
GCCACGTCCCTTTGGCAAGGAAGTTGAACAGTATTTACAGCGTTATCCGGATACCGAACACGTCGATATTTATCTCAACG
ATTTAAACGGTCAATTCCGTGGCAAACGGCTGCCGGTCGCCGAGTTGTTCGGGCTGGAAAAGGGCTGCTATTTCCCGCTG
TCGATCTACGCCATGGATCTCGCAGGCCAGGTCATTGAAGAAAGCGGGCTGGGTCAGCAGACCGGGGAGCCGGACAGGTT
GTGCCTGCCGGTACCGGGCACATTGCGTCCCTGCGCCCGTGACCCACAGCACCACGCTCAGTTGCTGCTGACGATGAAAA
ATGCCGAAGGTGAGGCCTGCGAACTGGAACCGCGCGTGGTGCTGCAACAGATTCTCAAACGCCTGCAGGCCAAAGGCTAC
TACCCGGTGATCGCGGCAGAACTGGAGTTTTATCTGCATGATCCGCAGCAACAGCCCGCCGTTGCCGGCCAGTGCCCGAC
GCAAAGTTTTTCTGTCGATGCCCCGGAACGCCACCACGCCCTGCTCAACGACATCGAGCATCAGGCCAGACTGCAGGCGC
TGCCGCTAACCGGCGTGGTGGCCGAGGCGGCCTCCGGTCAGTACGAACTGAACCTGCACCACAGTGCCCGGGTGCTGGAA
GCCTGCGACCAGGTGCTGGCCCTGAAACGGCTAACGCGCCAGATAGCGGAGAAACACCACCAGCACGCCTGCTTTATGGC
CAAACCCTGCGCGCAATCCGCCGGCAGCGGTTTGCACTTTCATATCAGCCTGCAGGATGAACAGGGCAACAACCTGCTGA
CCGGCGCTCCGGGCGAACTGAGCGACAACATGCAACAGGCGATGGCCGGAATGCTGGCATTGATGCCGGCCTCGATGGCC
ATCCTGGCACCGAACGTCAACGCCTTCCGCCGTTTTCGCCCGGGTATGCATGTGCCACTGCGTGCCTCCTGGGGGCACAA
CAACCGCACCGTCGCACTACGCCTGCCCTGTGCCGACAGCGCCAACCAACGTATTGAATACCGGTTGGCCGGGGCCGACG
CCAATCCTTATCTGGCGGTGGCGATAATACTCGGCGGCCTATTGCACGGGCTGGAGCAGCCCCTGCCGTTGCCCCCGAGC
ACCAACGGCTATGAAAACGACAGCGCCGCACCACTGCCACTCAACCAGCGGGAGGCACTTACGCTGTTTCGCCGCTGTCA
GCCGCTGCGCGAGCTGCTTGGCCCGGCCTTTTGCACCCTGTGGTACACCTGCAAAACCGCCGAACTGCGCCGCTTTGAAG
GGCAGGTCACCGCCGCGGAAGTCAGTTGGATGCTTTAA

Upstream 100 bases:

>100_bases
GACGCATTATTTACATTTTTTTTATTCCGGGCGGAAATAAAGTTACTGCGTTTTTACAGACAGTTTTTAGGGCCACGGAT
AACATCATTACATCGCCAAT

Downstream 100 bases:

>100_bases
CCCGCCGGGTTCTGCATTCATCGGGCGAATATATTCGCCCCATAATTGAACAAGGAACAAAACATGTCTCGTTTGGTTTT
GATCTCGAATAAACAGTGTG

Product: glutamine synthetase catalytic region

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MASRDGCFMYSNIIGFNPMLADITAAQPRPFGKEVEQYLQRYPDTEHVDIYLNDLNGQFRGKRLPVAELFGLEKGCYFPL
SIYAMDLAGQVIEESGLGQQTGEPDRLCLPVPGTLRPCARDPQHHAQLLLTMKNAEGEACELEPRVVLQQILKRLQAKGY
YPVIAAELEFYLHDPQQQPAVAGQCPTQSFSVDAPERHHALLNDIEHQARLQALPLTGVVAEAASGQYELNLHHSARVLE
ACDQVLALKRLTRQIAEKHHQHACFMAKPCAQSAGSGLHFHISLQDEQGNNLLTGAPGELSDNMQQAMAGMLALMPASMA
ILAPNVNAFRRFRPGMHVPLRASWGHNNRTVALRLPCADSANQRIEYRLAGADANPYLAVAIILGGLLHGLEQPLPLPPS
TNGYENDSAAPLPLNQREALTLFRRCQPLRELLGPAFCTLWYTCKTAELRRFEGQVTAAEVSWML

Sequences:

>Translated_465_residues
MASRDGCFMYSNIIGFNPMLADITAAQPRPFGKEVEQYLQRYPDTEHVDIYLNDLNGQFRGKRLPVAELFGLEKGCYFPL
SIYAMDLAGQVIEESGLGQQTGEPDRLCLPVPGTLRPCARDPQHHAQLLLTMKNAEGEACELEPRVVLQQILKRLQAKGY
YPVIAAELEFYLHDPQQQPAVAGQCPTQSFSVDAPERHHALLNDIEHQARLQALPLTGVVAEAASGQYELNLHHSARVLE
ACDQVLALKRLTRQIAEKHHQHACFMAKPCAQSAGSGLHFHISLQDEQGNNLLTGAPGELSDNMQQAMAGMLALMPASMA
ILAPNVNAFRRFRPGMHVPLRASWGHNNRTVALRLPCADSANQRIEYRLAGADANPYLAVAIILGGLLHGLEQPLPLPPS
TNGYENDSAAPLPLNQREALTLFRRCQPLRELLGPAFCTLWYTCKTAELRRFEGQVTAAEVSWML
>Mature_464_residues
ASRDGCFMYSNIIGFNPMLADITAAQPRPFGKEVEQYLQRYPDTEHVDIYLNDLNGQFRGKRLPVAELFGLEKGCYFPLS
IYAMDLAGQVIEESGLGQQTGEPDRLCLPVPGTLRPCARDPQHHAQLLLTMKNAEGEACELEPRVVLQQILKRLQAKGYY
PVIAAELEFYLHDPQQQPAVAGQCPTQSFSVDAPERHHALLNDIEHQARLQALPLTGVVAEAASGQYELNLHHSARVLEA
CDQVLALKRLTRQIAEKHHQHACFMAKPCAQSAGSGLHFHISLQDEQGNNLLTGAPGELSDNMQQAMAGMLALMPASMAI
LAPNVNAFRRFRPGMHVPLRASWGHNNRTVALRLPCADSANQRIEYRLAGADANPYLAVAIILGGLLHGLEQPLPLPPST
NGYENDSAAPLPLNQREALTLFRRCQPLRELLGPAFCTLWYTCKTAELRRFEGQVTAAEVSWML

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=433, Percent_Identity=24.0184757505774, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI87081870, Length=470, Percent_Identity=51.063829787234, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1790301, Length=175, Percent_Identity=30.2857142857143, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 51403; Mature: 51271

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRDGCFMYSNIIGFNPMLADITAAQPRPFGKEVEQYLQRYPDTEHVDIYLNDLNGQFR
CCCCCCCEEEHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GKRLPVAELFGLEKGCYFPLSIYAMDLAGQVIEESGLGQQTGEPDRLCLPVPGTLRPCAR
CCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCC
DPQHHAQLLLTMKNAEGEACELEPRVVLQQILKRLQAKGYYPVIAAELEFYLHDPQQQPA
CHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCC
VAGQCPTQSFSVDAPERHHALLNDIEHQARLQALPLTGVVAEAASGQYELNLHHSARVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECHHHHHHH
ACDQVLALKRLTRQIAEKHHQHACFMAKPCAQSAGSGLHFHISLQDEQGNNLLTGAPGEL
HHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHCCCCEEEEEEEECCCCCEEEECCCCHH
SDNMQQAMAGMLALMPASMAILAPNVNAFRRFRPGMHVPLRASWGHNNRTVALRLPCADS
HHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCC
ANQRIEYRLAGADANPYLAVAIILGGLLHGLEQPLPLPPSTNGYENDSAAPLPLNQREAL
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
TLFRRCQPLRELLGPAFCTLWYTCKTAELRRFEGQVTAAEVSWML
HHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCEEEEEEEECC
>Mature Secondary Structure 
ASRDGCFMYSNIIGFNPMLADITAAQPRPFGKEVEQYLQRYPDTEHVDIYLNDLNGQFR
CCCCCCEEEHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCC
GKRLPVAELFGLEKGCYFPLSIYAMDLAGQVIEESGLGQQTGEPDRLCLPVPGTLRPCAR
CCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCC
DPQHHAQLLLTMKNAEGEACELEPRVVLQQILKRLQAKGYYPVIAAELEFYLHDPQQQPA
CHHHHEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCC
VAGQCPTQSFSVDAPERHHALLNDIEHQARLQALPLTGVVAEAASGQYELNLHHSARVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECHHHHHHH
ACDQVLALKRLTRQIAEKHHQHACFMAKPCAQSAGSGLHFHISLQDEQGNNLLTGAPGEL
HHHHHHHHHHHHHHHHHHHHHCEEEEECHHHHHCCCCEEEEEEEECCCCCEEEECCCCHH
SDNMQQAMAGMLALMPASMAILAPNVNAFRRFRPGMHVPLRASWGHNNRTVALRLPCADS
HHHHHHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCCCEEEECCCCCCCEEEEEECCCCC
ANQRIEYRLAGADANPYLAVAIILGGLLHGLEQPLPLPPSTNGYENDSAAPLPLNQREAL
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
TLFRRCQPLRELLGPAFCTLWYTCKTAELRRFEGQVTAAEVSWML
HHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]